• Title/Summary/Keyword: ER sensing

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Endoplasmic Reticulum Ca2+ Store: Regulation of Ca2+ Release and Reuptake by Intracellular and Extracellular Ca2+ in Pancreatic Acinar Cells

  • Kang, Yun Kyung;Park, Myoung Kyu
    • Molecules and Cells
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    • v.19 no.2
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    • pp.268-278
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    • 2005
  • We investigated the effect of cytosolic and extracellular $Ca^{2+}$ on $Ca^{2+}$ signals in pancreatic acinar cells by measuring $Ca^{2+}$ concentration in the cytosol($[Ca^{2+}]_c$) and in the lumen of the ER($[Ca^{2+}]_{Lu}$). To control buffers and dye in the cytosol, a patch-clamp microelectrode was employed. Acetylcholine released $Ca^{2+}$ mainly from the basolateral ER-rich part of the cell. The rate of $Ca^{2+}$ release from the ER was highly sensitive to the buffering of $[Ca^{2+}]_c$ whereas ER $Ca^{2+}$ refilling was enhanced by supplying free $Ca^{2+}$ to the cytosol with $[Ca^{2+}]_c$ clamped at resting levels with a patch pipette containing 10 mM BAPTA and 2 mM $Ca^{2+}$. Elevation of extracellular $Ca^{2+}$ to 10 mM from 1 mM raised resting $[Ca^{2+}]_c$ slightly and often generated $[Ca^{2+}]_c$ oscillations in single or clustered cells. Although pancreatic acinar cells are reported to have extracellular $Ca^{2+}$-sensing receptors linked to phospholipase C that mobilize $Ca^{2+}$ from the ER, exposure of cells to 10 mM $Ca^{2+}$ did not decrease $[Ca^{2+}]_{Lu}$ but rather raised it. From these findings we conclude that 1) ER $Ca^{2+}$ release is strictly regulated by feedback inhibition of $[Ca^{2+}]_c$, 2) ER $Ca^{2+}$ refilling is determined by the rate of $Ca^{2+}$ influx and occurs mainly in the tiny subplasmalemmal spaces, 3) extracellular $Ca^{2+}$-induced $[Ca^{2+}]_c$ oscillations appear to be triggered not by activation of extracellular $Ca^{2+}$-sensing receptors but by the ER sensitised by elevated $[Ca^{2+}]_c$ and $[Ca^{2+}]_{Lu}$.

Position estimation and control of SMA actuators based on electrical resistance measurement

  • Song, Gangbing;Ma, Ning;Lee, Ho-Jun
    • Smart Structures and Systems
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    • v.3 no.2
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    • pp.189-200
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    • 2007
  • As a functional material, shape memory alloy (SMA) has attracted much attention and research effort to explore its unique properties and its applications in the past few decades. Some of its properties, in particular the electrical resistance (ER) based self-sensing property of SMA, have not been fully studied. Electrical resistance of an SMA wire varies during its phase transformation. This variation is an inherent property of the SMA wire, although it is highly nonlinear with hysteresis. The relationship between the displacement and the electrical resistance of an SMA wire is deterministic and repeatable to some degree, therefore enabling the self-sensing ability of the SMA. The potential of this self-sensing ability has not received sufficient exploration so far, and even the previous studies in literature lack generality. This paper concerns the utilization of the self-sensing property of a spring-biased Nickel-Titanium (Nitinol) SMA actuator for two applications: ER feedback position control of an SMA actuator without a position sensor, and estimation of the opening of a SMA actuated valve. The use of the self-sensing property eliminates the need for a position sensor, therefore reducing the cost and size of an SMA actuator assembly. Two experimental apparatuses are fabricated to facilitate the two proposed applications, respectively. Based on open-loop testing results, the curve fitting technique is used to represent the nonlinear relationships between the displacement and the electrical resistance of the two SMA wire actuators. Using the mathematical models of the two SMA actuators, respectively, a proportional plus derivative controller is designed for control of the SMA wire actuator using only electrical resistance feedback. Consequently, the opening of the SMA actuated valve can be estimated without using an extra sensor.

Nondestructive Damage Sensing and Cure Monitoring of Carbon Fiber/Epoxyacrylate Composite with UV and Thermal Curing using Electro-Micromechanical Technique (Electro-Micromechanical 시험법을 이용한 탄소섬유 강화 Epoxyacrylate 복합재료의 UV 및 열경화에 따른 비파괴적 손상 감지능 및 경화 Monitoring)

  • Kong, Jin-Woo;Kim, Dae-Sik;Park, Joung-Man;Lee, Jae-Rock
    • Proceedings of the Korean Society For Composite Materials Conference
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    • 2002.10a
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    • pp.261-264
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    • 2002
  • Interfacial evaluation, damage sensing and cure monitoring of single carbon fiber/thermosetting composite with different curing processes was investigated using electro-micromechanical test. After curing, residual stress was monitored by measurement of electrical resistance (ER) and then it was compared to correlate with various curing processes. In thermal curing, curing shrinkage appeared significantly by matrix shrinkage and residual stress due to the difference in thermal expansion coefficient (TEC). The change in electrical resistance (ΔR) on thermal curing was higher than that on ultraviolet (UV) curing. For thermal curing, apparent modulus was the highest and reaching time until same strain was faster. So far thermal curing shows strong durability on the IFSS after boiling test.

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The Study of MAC protocol for efficient Wireless Sensor Network (Wireless 센서 네트워크를 위한 MAC 프로토콜에 관한 연구)

  • Lee, Woo-Chul;Kim, Dong-Il
    • Proceedings of the Korean Institute of Information and Commucation Sciences Conference
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    • v.9 no.2
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    • pp.517-520
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    • 2005
  • Wireless sensor network combines sensing and computing technology which can sense light, temperature, vibration, magnetic field and wind etc, as each purpose of using those. Wireless nodes operate signal processing skill which has proceeded sensed information from the sensor, transmission which makes information reached to observer and limited energy managing skill which is needed on account of using battery to operate wireless. To make responsible measuring and sensing out of them, efficient energy management is so important to maintain life time of network. In this paper, after explaining CSMA/CA(Carrier Sense Multiple Access/Collision Avoidance) traditional wireless MAC protocol, and ER-MAC(Energy Rate Medium Access Control) which are not managing resource of hardware but MAN(Medium Access Control), data-link layer out of OSI 7 layer. We would like to analyze those efficiency of power saving comparing with each protocol.

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Thermomechanical and electrical resistance characteristics of superfine NiTi shape memory alloy wires

  • Qian, Hui;Yang, Boheng;Ren, Yonglin;Wang, Rende
    • Smart Structures and Systems
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    • v.30 no.2
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    • pp.183-193
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    • 2022
  • Structural health monitoring and structural vibration control are multidisciplinary and frontier research directions of civil engineering. As intelligent materials that integrate sensing and actuation capabilities, shape memory alloys (SMAs) exhibit multiple excellent characteristics, such as shape memory effect, superelasticity, corrosion resistance, fatigue resistance, and high energy density. Moreover, SMAs possess excellent resistance sensing properties and large deformation ability. Superfine NiTi SMA wires have potential applications in structural health monitoring and micro-drive system. In this study, the mechanical properties and electrical resistance sensing characteristics of superfine NiTi SMA wires were experimentally investigated. The mechanical parameters such as residual strain, hysteretic energy, secant stiffness, and equivalent damping ratio were analyzed at different training strain amplitudes and numbers of loading-unloading cycles. The results demonstrate that the detwinning process shortened with increasing training amplitude, while austenitic mechanical properties were not affected. In addition, superfine SMA wires showed good strain-resistance linear correlation, and the loading rate had little effect on their mechanical properties and electrical resistance sensing characteristics. This study aims to provide an experimental basis for the application of superfine SMA wires in engineering.

Design and Implementation of Body-fat sensing Node(BSN) (체지방 센서 노드(BSN) 설계 및 구현)

  • Kim, Youn-Seo;Hong, Sung-Woong;Kim, Pyeong-Jung
    • Proceedings of the Korea Information Processing Society Conference
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    • 2010.11a
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    • pp.1564-1566
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    • 2010
  • 본 논문에서는 유헬스 시스템에 가장 기본이 되는 생체정보 측정에 대하여 하나의 휴대용 인터페이스만을 가지고 다양한 생체정보 측정기와 연계하여 다양한 생체정보를 취득할 수 있도록 하는 이식성 있는 임베디드 미들웨어로써 특히 체지방측정을 위한 체지방 센서 노드(BSN)의 설계 및 구현에 관한 것이다. BSN은 U-health 서비스를 제공하기 위해 측정기 자동인식, 데이터 취득, 위험요소 표시 및 데이터 수집장치(DAS)와의 통신을 담당하는 기능을 갖는다.

ASIC2a-dependent increase of ASIC3 surface expression enhances the sustained component of the currents

  • Kweon, Hae-Jin;Cho, Jin-Hwa;Jang, Il-Sung;Suh, Byung-Chang
    • BMB Reports
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    • v.49 no.10
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    • pp.542-547
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    • 2016
  • Acid-sensing ion channels (ASICs) are proton-gated cation channels widely expressed in the nervous system. Proton sensing by ASICs has been known to mediate pain, mechanosensation, taste transduction, learning and memory, and fear. In this study, we investigated the differential subcellular localization of ASIC2a and ASIC3 in heterologous expression systems. While ASIC2a targeted the cell surface itself, ASIC3 was mostly accumulated in the ER with partial expression in the plasma membrane. However, when ASIC3 was co-expressed with ASIC2a, its surface expression was markedly increased. By using bimolecular fluorescence complementation (BiFC) assay, we confirmed the heteromeric association between ASIC2a and ASIC3 subunits. In addition, we observed that the ASIC2a-dependent surface trafficking of ASIC3 remarkably enhanced the sustained component of the currents. Our study demonstrates that ASIC2a can increase the membrane conductance sensitivity to protons by facilitating the surface expression of ASIC3 through herteromeric assembly.

Development of Human-machine Interface based on EMG and EOG (근전도와 안전도 기반의 인간-기계 인터페이스기술)

  • Gang, Gyeong Woo;Kim, Tae Seon
    • Journal of the Institute of Electronics and Information Engineers
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    • v.50 no.12
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    • pp.129-137
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    • 2013
  • As the usage of computer based systems continues to increase in our normal life, there are constant efforts to enhance the accessibility of information for handicapped people. For this, it is essential to develop new interface ways for physical disabled peoples by means of human-computer interface (HCI) or human-machine interface (HMI). In this paper, we developed HMI using electromyogram (EMG) and electrooculogram (EOG) for people with physical disabilities. Developed system is composed of two modules, hardware module for signal sensing and software module for feature extraction and pattern classification. To maximize ease of use, only two skin contact electrodes are attached on both ends of brow, and EOG and EMG are measured simultaneously through these two electrodes. From measured signal, nine kinds of command patterns are extracted and defined using signal processing and pattern classification method. Through Java based real-time monitoring program, developed system showed 92.52% of command recognition rate. In addition, to show the capability of the developed system on real applications, five different types of commands are used to control ER1 robot. The results show that developed system can be applied to disabled person with quadriplegia as a novel interface way.

Remote Measurement of a Distant Temperature and Current using Fiber Bragg Grating Sensors and Erbium-doped Fiber Ring Laser (어븀 첨가 광섬유형 링 레이저와 광섬유 격자 기반 센서를 이용한 원거리의 온도 및 전류 측정)

  • Sohn, Kyung-Rak;Shim, June-Hwan;Yang, Gyu-Sik
    • Journal of Advanced Marine Engineering and Technology
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    • v.32 no.8
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    • pp.1257-1262
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    • 2008
  • A long-distance remote sensing of temperature and current based on a fiber Bragg grating (FBG) is proposed and demonstrated. The thermal expanding effect of the epoxy and the Er-doped fiber ring laser (EFRL) are applied to the sensor system to enhance the temperature and current sensitivity. An EFRL with a 5 km-single-mode fiber and a FBG shows a high extinction ratio of more than 60 dB and a low power fluctuation of less than 1 dB. The metal wires are used to supply the current to the sensors. When the NOA65 puts on the FBG as a thermal expanding material, the temperature and current sensitivity of the lasing wavelength shift are about $30\;pm/^{\circ}C$ and 3pm/mA, respectively. The proposed sensing scheme is useful for measurement of current or temperature at a distant object of more than several km.

Strain Sensing of Single Lap Shear using Pencil Lead Drawn Paper Sensor (PLDPS) (연필심을 이용한 종이센서에 의한 단일 랩 전단변형률 감지능)

  • Yoo, Ji-Hoon;Shin, Pyeong-Su;Kim, Jong-Hyun;Lee, Sang-Il;Park, Joung-Man
    • Composites Research
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    • v.33 no.4
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    • pp.228-233
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    • 2020
  • In this paper, a single lap shear test was performed using a glass fiber reinforced composite material (GFRC). Pencil lead drawn paper sensor (PLDPS) was applied for single lap shear test being performed. Bisphenol-A epoxy and amine hardener were used as adhesives combining with composite materials. To make a difference in adhesive properties, the adhesive was cured under different conditions. PLDPS was made of a 4B pencil on A4 paper. Because graphite in a pencil was an electrically conductive substance, electric resistance (ER) could be measured. A change in ER was observed by a position where a PLDPS was attached to single lap shear specimens. It was confirmed that the change in ER was different depending on two attached positions and was observed by lap shear strain as well. In case the lap shear strain was large, the change in ER of PLDPS was high. This was because the larger the extension of the adhesive part, the larger the degree of bending of the specimen and thus the larger the distance change between two electrodes.