• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2000년도 추계학술대회 논문집
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• pp.850-853
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• 2000

It is well-known that Electro-rheological(ER) fluid is a material(suspension) which shows the dramatic change of rheological properties under an electric field. Using these properties, the concept that variable apparent viscosity of ER fluid could be applicable to the polishing for micro parts was introduced. It was investigated that how it works for polishing and how it affects ER effect when abrasives were mixed with an ER fluid. Therefore a few structures for polishing using ER fluid was suggested and evaluated by means of experiments. In this paper, fundamental mechanism and experimental results are described.

• 한국기계가공학회지
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• 제9권1호
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• pp.1-7
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• 2010

A new flow mode of ER fluid available for controlling the damping force by using DME(Discrete Multi-Electrode) is presented in this study. Various characteristics about the flow of ER fluid through the experiment of ER cluster behavior visualization can be assumed. The pressure in electrode length and voltage division mode is measured. An actuator with a damping effect through DME ER damper will be developed. This damper controls the damping force by using the displacement and velocity of the plant which consists of the various electrode length and voltage modes without a controller in the real system.

• 한국윤활학회:학술대회논문집
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• 한국윤활학회 1994년도 제20회 학술대회
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• pp.19-29
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• 1994

Since Winslow (1) has reported an electro - theological (ER) effect which features remarkable and reversible changes in the properties of the fluid due to an imposed external electric field, numerous applications of ER fluids in mechanical devices, such as clutches, control valves, active dampers, and etc. have been proposed to improye dramatical ly their performances (2,3). When the external electric field is imposed to the ER fluid, it behaves as a Bingham fluid, displaying a field dependent yield shear stress which is widely variable. Without the electric field, the ER fluid has a reversible and constant viscosity so that it flows as a Newtonian fluid. Another salient feature of the ER fluid is that the time required for the variation is very short (< 0.001 sec) (4-6). These attractive.characteristics of the ER fluid provide the possibility of the appearance of new engineering technology , for instance, an active vibration control system. Recently, the application of the ER fluid to rotor-bearing systems has been also initiated.

• 대한의용생체공학회:의공학회지
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• 제20권2호
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• pp.231-236
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• 1999

Q-switch된 Er:YAG 레이저를 이용해서 치아표면에 뿜어준 외부의 물로 인해 유도된 열 및 역학적인 효과를 이해하는 것은 free-running Er:YAG 레이저에 의한 절삭효과를 이해하는데 대단히 중요하다. 이는 (파장 하나의 길이가 250 $\mu\textrm{s}$인) free-running 레이저에 의한 한 거 대효과가 본 실험에서 이용한 (파장 하나의 길이가 1 $\mu\textrm{s}$인) Q-switch된 레이저에 의한 미소효과들의 축적이기 때문이다. 치아표면에 물이 있을 때 Q-Switch된 레이저에 의한 절삭률은 그렇치 않은 때에 비해 증가되었다. 치아표면에 물을 뿜어주는 횟수는 절삭률에 영향을 미쳐서 저에너지에서는 더디게 물을 뿜어줄때 놀은 절삭률을 얻었다. 반동압력의 세기는 치아표면 조건에 의존하는데. 표면이 젖을수록 그리고 뿜어준 물의 양이 증가 할 수록, 반동압력의 세기 또한 증가하였다. 이 연구로부터 우리는 1-$\mu\textrm{s}$-길이의 pulsed 레이저에 의해 유도된 열 및 역학적인 효과가 외부에 물이 있을 때 free-running Er:YAG 레이저에 의한 절삭을 이해하는데 중요한 정보를 제공해 준다는 것을 알았다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권12호
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• pp.1684-1690
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• 2014

Estrogen and its receptors are essential hormones for normal reproductive function in males and females during developmental stage. To better understand the effect of estrogen receptor (ER) gene in yak (Bos grunniens), reverse transcription-polymerase chain reaction (PCR) was carried out to clone $ER{\alpha}$ and $ER{\beta}$ genes. Bioinformatics methods were used to analyze the evolutionary relationship between yaks and other species, and real-time PCR was performed to identify the mRNA expression of $ER{\alpha}$ and $ER{\beta}$. Sequence analysis showed that the ER open reading frames (ORFs) encoded 596 and 527 amino acid proteins. The yak $ER{\alpha}$ and $ER{\beta}$ shared 45.3% to 99.5% and 53.9% to 99.1% protein sequence identities with other species homologs, respectively. Real-time PCR analysis revealed that $ER{\alpha}$ and $ER{\beta}$ were expressed in a variety of tissues, but the expression level of $ER{\alpha}$ was higher than that of $ER{\beta}$ in all tissues, except testis. The mRNA expression of $ER{\alpha}$ was highest in the mammary gland, followed by uterus, oviduct, and ovary, and lowest in the liver, kidney, lung, testis, spleen, and heart. The $ER{\beta}$ mRNA level was highest in the ovary; intermediary in the uterus and oviduct; and lowest in the heart, liver, spleen, lung, kidney, mammary gland, and testis. The identification and tissue distribution of ER genes in yaks provides a foundation for the further study on their biological functions.

• 한국발생생물학회지:발생과생식
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• 제22권3호
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• pp.235-244
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• 2018

We investigate the effect of endoplasmic reticulum (ER) stress inhibitor treatment during parthenogenetic activation of oocytes on the ER stress generation, apoptosis, and in vitro development of parthenogenetic porcine embryos. Porcine in vitro matured oocytes were activated by 1) electric stimulus (E) or 2) $E+10{\mu}M$ Ca-ionophore (A23187) treatment (EC). Oocytes were then treated by ER stress inhibitors such as salubrinal (200 nM) and tauroursodeoxychloic acid (TUDCA, $100{\mu}M$) for 3 h prior to in vitro culture. Parthenogenetic embryos were sampled to analyze ER stress and apoptosis at the 1-cell and blastocyst stages. The x-box binding protein 1 (Xbp1) mRNA and ER stress-associated genes were analyzed by RT-PCR or RT-qPCR. Apoptotic gene expression was analyzed by RT-PCR. At the 1-cell stage, although no difference was observed in Xbp1 splicing among treatments, BiP transcription level in the E group was significantly reduced by salubrinal treatment, and GRP94 and ATF4 transcription levels in EC group were significantly reduced by all treatments (p<0.05) compared to control. In the EC group, both apoptotic genes were reduced by ER stress inhibitor treatments compared to control (p<0.05) except Caspase-3 gene by TUDCA treatment. These results suggest that the treatment of ER stress inhibitor during parthenogenetic activation can reduce ER stress, and thereby reduce apoptosis and promote in vitro development of porcine parthenogenetic embryos.

• Molecules and Cells
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• 제38권2호
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• pp.145-150
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• 2015

Continuous intra- and extracellular stresses induce disorder of $Ca^{2+}$ homeostasis and accumulation of unfolded protein in the endoplasmic reticulum (ER), which results in ER stress. Severe long-term ER stress triggers apoptosis signaling pathways, resulting in cell death. Neural epidermal growth factor-like like protein 2 (NELL2) has been reported to be important in protection of cells from cell death-inducing environments. In this study, we investigated the cytoprotective effect of NELL2 in the context of ER stress induced by thapsigargin, a strong ER stress inducer, in Cos7 cells. Overexpression of NELL2 prevented ER stress-mediated apoptosis by decreasing expression of ER stress-induced C/EBP homologous protein (CHOP) and increasing ER chaperones. In this context, expression of anti-apoptotic Bcl-xL was increased by NELL2, whereas NELL2 decreased expression of pro-apoptotic proteins, such as cleaved caspases 3 and 7. This anti-apoptotic effect of NELL2 is likely mediated by extracellular signal-regulated kinase (ERK) signaling, because its inhibitor, U0126, inhibited effects of NELL2 on the expression of anti- and pro-apoptotic proteins and on the protection from ER stress-induced cell death.

• Biomolecules & Therapeutics
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• 제15권4호
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• pp.240-244
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• 2007

Cigarette smoking causes serious health problems in humans, especially if smoking habits are established during their adolescence. Nicotine is known to mutate DNA and interfere with apoptosis. Apoptosis is considered as a potent defense mechanism against cellular damaging agents. This study aims to investigate the effect of nicotine on the progression of apoptosis induced under ER stress conditions using four different established cell lines: HEK293, 3T3-L1, C2C12, and HepG2. When treated with nicotine, the progression of apoptosis was notably inhibited in the four cell lines according to the assays of caspase-3 activation and DNA fragmentation. In ER-stressed cells, nicotine appears to inhibit the progression of apoptosis in a concentration-dependent manner. When cells were treated with nicotine prior to ER stress, GRP94 level significantly increased compared to other ER stress markers of PDI and GRP78. This observation suggests that the inhibitory effect of nicotine may results from up-regulation of GRP94, an anti-apoptotic chaperone, under nicotine treatment. Taken together, the present study strongly implies that nicotine may inhibit apoptosis, caused by prolonged ER stress, based on promotion of GRP94 expression.

• Journal of Ceramic Processing Research
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• 제20권2호
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• pp.182-186
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• 2019

0.7 mol% Er-TiO2 nanotubes were prepared using a sol-gel derived electrospinning and subsequent calcination at intervals of 50 ℃ from 500 ℃ to 650 ℃ to investigate the effect of calcination temperature on the crystal structure and the photocatalytic activity of methylene blue (MB). X-ray diffraction (XRD) results indicated that Er-TiO2 nanotubes calcined at 500 ℃ were composed of anatase only. However, mixed phases of anatase (51%, 55%, 96%) and rutile (49%, 45%, 4%) were observed for the nanotubes calcined at 550 ℃, 600 ℃ and 650 ℃, respectively. As the calcination temperature rose from 500 ℃ to 600 ℃, the Barrette-Emmett-Teller (BET) surface area and degradation kinetic constant increased from 97.77 ㎡/g to 117.62 ㎡/g and from 1.2 × 10-2min-1 to 1.6 × 10-2 min-1, respectively. The Er-TiO2 nanotubes calcined at 600 ℃ exhibited enhanced MB degradation (87%) compared to that of Er-TiO2 nanofibers (37%) due to the synergic combinations of tailored mixed crystals and larger BET area.

• KSTLE International Journal
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• 제9권1_2호
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• pp.7-9
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• 2008

The electrorheology of the HMDA coupled chitosan succinate suspension in silicone oil was investigated. HMDA coupled chitosan succinate suspension showed a typical ER response upon application of an electric field. The shear stress for the HMDA coupled chitosan succinate suspension exhibited an electric field power of 2.0. The experimental results for the HMDA coupled chitosan succinate suspension was found to be an anhydrous ER fluid.