• Journal of Physiology & Pathology in Korean Medicine
• /
• v.26 no.3
• /
• pp.287-292
• /
• 2012

The results of this study intended to clarify the apoptotic effects of curcumin on Epstein-Barr virus transformed human B lymphoma (EBV-B) cells are summarized as follows: It was found that curcumin induced endoplasmic reticulum(ER) stress as well as apoptotic cell death in EBV-B cells, although the magnitude of action was insignificant. When EBV-B cells activated by pokeweed mitogen (PWM) were treated with the same concentrations of curcumin, it was found that higher ER stress (GRP78, P-PERK, XBP-1, ATF6, and CHOP expressed) increased unfold protein response (UPR) and thus, apoptosis attributed to ER stress, compared to non-activated EBV-B cells In conclusion, it is expected that curcumin will play an important role for leukemia treatment.

• Proceedings of the Korean Vacuum Society Conference
• /
• 2002.06a
• /
• pp.93-93
• /
• 2002

• Journal of Life Science
• /
• v.28 no.10
• /
• pp.1186-1192
• /
• 2018

The purpose of this study was to compare the effects of either aerobic exercise or polyphenols supplementation on mRNA expression of endoplasmic reticulum stress in skeletal muscle of high fat diet-induced obese mice. In the study, mice were divided into five groups: (1) NC (normal diet for 16 weeks as a control, n=10), (2) HC (high fat diet for 16 weeks as a control, n=10), (3) H-Re (high fat diet with resveratrol 25 mg/kg supplementation for 16 weeks, n=10), (4) H-Ch (high fat diet with chrysin 50 mg/kg supplementation for 16 weeks, n=10), and (5) HE (high fat diet with aerobic exercise for 16 weeks, n=10). Aerobic exercise was performed on a treadmill for 40~60 min/day at 10~14 m/min, 0% grade, four days/week for 16 weeks. Endoplasmic reticulum stress related genes were measured by real-time polymerase chain reaction. ATF6, PERK, $IRE1{\alpha}$, and BIP/GRP78 mRNA were significantly decreased in HE compared with those in HC (p<0.05). Also, ATF6, $IRE1{\alpha}$, and BIP/GRP78 mRNA were significantly decreased in H-Re compared with those in HC (p<0.05). ATF6 mRNA was significantly decreased in H-Ch compared with that in HC (p<0.05). These findings suggest that aerobic exercise, resveratrol, and chrysin supplementation changed ER stress markers. However, aerobic exercise was most effective on ameliorating the high fat diet induced ER stress markers. Thus, it seems that aerobic exercise might have a more positive effect on skeletal muscle endoplasmic reticulum stress compared with polyphenol supplementation in high fat diet-induced obese mice.

• Environmental Analysis Health and Toxicology
• /
• v.18 no.4
• /
• pp.249-254
• /
• 2003

본 연구는 환경호르몬(endocrine disruptors)으로 분류되었으며, 에스트로젠 화합물의 특성을 지닌 4-Nonylphenol (NP)이 설치류 Pituitary 세포 중 성장호르몬을 분비하는 GH3 세포의 Nitric oxide(NO)을 증가시키는 작용기전을 규명코자 수행되었다 먼저 GH3세포에 NP처리 농도에 따른 NO의 생성을 측정한 결과 NP처리농도 의존적으로 증가시켰다. 이러한 NO의 증가가 genomic action인지를 확인하기 위해 GH3세포의 NO를 증가시키는 효소인 neuronal oxide synthase의 단백질량을 측정한 결과 GH3세포에서 NP에 의한 nNOS의 단백질의 변화는 없었다. 에스트로젠 화합물인 NP가 에스트로젠 리셉터 (ER)와의 관계를 조사하기 위해 ER억제제(ICI 168,780)클 처리한 경우 NP에 의해 증가한 NO가 감소하였다. 또한 유전자 전사억제제인 actinomycin D 및 단백질 발현 억제제인 cycloheximide을 처리한 경우는 NP에 의한 NO 증가억제효과가 없었다. 이러한 결과를 종합해 볼 때 GH3 세포에서 NP는 ER을 매개한 non-genomic action에 의해 NO를 증가키는 것으로 사료된다.

• Proceedings of the KIEE Conference
• /
• 1998.11c
• /
• pp.726-728
• /
• 1998

Optical and electrical properties of GaSe:$Er^{3+}$ single crystals grown by the Bridgeman technique was been investigated by using optical absorption and Hall-effect measurements. The Hall coefficients were measured by using a high impedance electrometer in the temperature range from 360K to 150K. The temperature dependence of hole concentration shows the characteristic of a partially compensated p-type semiconductor. carrier density($N_H$) of GaSe doped with Erbium was measured about $3.25{\times}10^{16}\;[cm^{-3}}$ at temperature 300K, which was high than undoped specimen. Photon energy gap ($E_{gd}$) was measured about 1.7geV.

• Korean Journal of Optics and Photonics
• /
• v.8 no.2
• /
• pp.128-133
• /
• 1997

The effects of twist induced birefringence on the polarization properties of Er and Nd fiber laser with a linear type cavity were experimentally observed and theoretically analyzed. As the twist rate increases, the polarization states of the polarization eigenmodes are found to be always linear, and their frequencies gradually coincide with each other, and their polarization directions rotate at about half of twist rate at large twist rates.

• The Korean Journal of Rheology
• /
• v.6 no.1
• /
• pp.49-59
• /
• 1994

높은 전기장 하에서 다분산, 비구형 실리카/실리콘 오일 현탁액의 전기유변 (electrorheological, ER)현상을 살펴 보았다. 전기유변유체는 전기장 방향으로 사슬구조를 형 성하는 특성을 보이며 이것이 유변물성의 향상에 기여하는 것으로 알려졌다. 동적(dynamic) 상태 실험에서 전기장 하의 실리카 현탁액은 매우낮은 임계변형율(${\gamma}$c =0.1%)이상에서 비선 형 점탄성을 보였다. 저장탄성계수(G＇)는 변형율 변화에 손실탄성계수(G＂)는 매질의 점도 에 더 민감한 의존성을 보였다. 또한 겉보기 항복응력은 입자의 부피분율과 전기장에$\Phi$1.9E1.4 의 의존성을 보였는데 부피분율에 대한 의존성이 큰 이유는 0.1 이상의 부피분율에서 복합 사슬 구조 내의 입자들 간의 상호 정전효과가 지배적으로 나타나기 때문이라고 생각된다. 정상상태 실험에서는 부피분율이 크거나 높은 전기장 하에서 전단속도가 0.1sec-1 정도 이하 로 감소함에 따라 전단응력이 급겨히 증가하는 현상을 보였다. 그러므로 본질적인 동적 항 복응력을 얻기 위해서는 매우 낮은 전단속도 영역의 특이한 응력거동을 고려해야한다. 큰 전단속도 하에서는 hydrodynamic interaction의 영향으로 전단속도의 증가에 따라 전단응력 이 증가하였다. 이같은 전단응력의 거동을 계단전단실험으로 확인하였다.

• Culinary science and hospitality research
• /
• v.22 no.3
• /
• pp.212-223
• /
• 2016

The purpose of this study is to verify information spillover effects using returns of macroeconomic variables and hotel leisure stock index daily data from January 4, 2000 to December 30, 2015. The findings and implications of the research can be summarized as follows. First, based on time-varying AR(1)-GARCH(1,1) models no evidence of statistically significant conditional mean and volatility spillover effects from returns of macroeconomic variables on the hotel leisure stock index was observed. In addition, no evidence of price volatility spillover from macroeconomic variables on the hotel leisure market was observed. Second, it was discovered that there exists a significantly negative relationship between the return of ER and hotel leisure stock prices, but a positive relationship between the KOSPI and hotel leisure stock prices. Finally, the study also found that was a significantly positive relationship between the volatility of DUB and hotel leisure market, and an adversely negative relationship between the volatility of ER and hotel leisure market. The results of this study are expected to contribute by providing useful information for investment strategies, as well as for risk management for investors and managers.

• Applied Microscopy
• /
• v.36 no.2
• /
• pp.57-72
• /
• 2006

A protective effect of activated charcoal against the acute lead poisoning of kidney was studied in mice. Mice approximately 30 gm in weight were grouped into the control, lead acetate-treated. and the activated charcoal-treated after lead acetate groups. Lead acetate (60mg/kg) and activated charcoal (40mg/kg) were delivered orally. Serum BUN and creatine were measured and ultrastructural alteration of renal tissues were examined by electron microscopy. Activated charcoal were decreased the increase of serum BUN and Creatinine level induced by lead. Lead acetate-treated renal tissues were characterized by the loss of microvilli in the renal tubule tells, irregular nucleus, enlarged and reduced number of mitochodria, enlarged rough endoplasmic reticulum, loss of ribosomes. Cells treated with activated charcoal were similar to those of the control group. In conclusion, activated charcoal may protect the lead-induced toxicity on kidney.

• Journal of the Korean Society of Food Science and Nutrition
• /
• v.44 no.7
• /
• pp.993-999
• /
• 2015

For the purpose of investigating the in vitro antidiabetic activity of a medicinal herb and herb mixture extracts prepared through traditional antidiabetic prescription, this study examined ${\alpha}$-glucosidase inhibitory activity. Tyrosinase, a type I membrane glycoprotein, is synthesized and glycosylated in the endoplasmic reticulum (ER) and Golgi. The enzyme is subsequently transported to melanosomes, where it participates in melanogenesis. Previous studies showed that disruption of early ER N-glycan processing by an ${\alpha}$-glucosidase inhibitor suppresses tyrosinase enzymatic activity and melanogenesis. According to the results, most oriental medicinal herbal extracts were stronger than acarbose and N-butyldeoxynojirimycin, known as an ${\alpha}$-glucosidase inhibitor. Interestingly, ethyl acetate layer of enzyme hydrolyzed Cheongsimyeonjaeum had an inhibitory effect on melanin synthesis in B16F1 cells, although it did not inhibit tyrosinase activity directly. Together, ${\alpha}$-glucosidase inhibition activity could be used to evaluate anti-melanogenesis, although cross-checking with melanin inhibitory assay is recommended.