• Natural Product Sciences
• /
• v.21 no.1
• /
• pp.1-5
• /
• 2015

Activity-guided separation of the methylene chloride-soluble fraction of the leaves of Zanthoxylum schinifolium, resulted in the isolation of four coumarinoids (1 - 4), two triterpenoids (5, 6) and three fatty acid derivatives (7 - 9) as active principles. Their chemical structures were identified as collinin (1), 8-methoxyanisocoumarin (2), 7-(6'R-hydroxy-3',7'-dimethylocta-2',7'-dienyloxy)-coumarin (3), (E)-4-methly-6-(coumarin-7'-yloxy) hex-4-enal (4), lupeol (5), epi-lupeol (6), phytol (7), hexadec-3-enoic acid (8) and palmitic acid (9), on the basis of spectroscopic (1D, 2D and MS) data analyses and comparing with the data published in the literatures. Compounds 1 and 7 showed potent cytotoxicity against Jurkat T cells with $IC_{50}$ values of 45.58 and $47.51{\mu}M$, respectively. The others showed moderate activity with $IC_{50}$ values ranging around 80.58 to $85.83{\mu}M$, while the positive control, auraptene, possessed an $IC_{50}$ value of $55.36{\mu}M$.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.28 no.9
• /
• pp.1429-1434
• /
• 2004

Immunoassay is one of the important analytical methods for clinical diagnoses and biochemical studies, but needs a long time, troublesome procedures and expensive reagents. In this study, therefore, we propose the micro filter chip with microbeads for immunoassay, which has pillar structures. The advantage of the proposed micro filter chip is to use simple fabrication process and cheap materials. The mold was made by the photolithography technique with Si wafer and negative photoresist SU-8. The replica was made of PDMS, bonded on the pyrex glass. The micro filter chip consists of inlet channel, filter chamber and outlet channel. HBV (Hepatitius B virus) monoclonal antibody (Ag1) labeled with biotin were immobilized onto streptavidin coated beads of 30∼50 $\mu$m size. Fluorescein isothiocyanate (FITC)-labeled HBV monoclonal antibody (Ag8) was used to detect HBsAg (Hebatitis B virus surface Antigen), and fluorescence intensity was monitored by epi-fluorescence microscope. In this study, the immune response of less than 30 min was obtained with with the use of 100 $m\ell$ of sample.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
• /
• v.25 no.12
• /
• pp.11-20
• /
• 2011

PSALI is referred to the supplementary lighting for the interior lighting under the daily lighting situation, and pursuant to the pertinent regulations in energy savings design standard and others in recent architecture works, the importance thereof has been increasing gradually coupled with the energy performance index (EPI), energy savings plan and the like as well as expansion of submittal and implementation policies. However, this type of PSALI studies indeed have a number of limitations since it has surrounding environmental conditions in direction, season, region, climate, time, opening rate, window area ratio, actual index, reflection rate of finishing materials and others in the architecture work as well as frequent changes in interior lighting environment for variables in daily light volume flowing into the interior, and others. Therefore, this study has analyzed existing advance research cases to produce the actual-sized model and scaled-down model, and installed the artificial lighting of LED light source possible to reproduce with same capability on both models. As a result of comparison and analysis of the artificial lighting with the key light, it has certain level of error rate from the scaled down lighting device in certain rate and actual model butit was noticeably significant within specific scope.

• Journal of Biomedical Engineering Research
• /
• v.28 no.4
• /
• pp.477-483
• /
• 2007

We have measured EOG and fMRI simultaneously to investigate whether eye movement (blinking mechanism) might influence functional magnetic resonance imaging (fMRI) signal response in the primary visual cortex. $T2^*-weighted$ Echo-Planar Imaging (EPI) with concurrent electrooculogram (EOG) was acquired in four subjects while they viewed a fixation point and a checkerboard with a flickering rate of 8Hz. With the help of EOG information we divided the experimental blocks into two different conditions: fixation and moving eye. We have compared the fMRI data of these two conditions. Our results have shown that there is no difference between these two conditions. This might suggest that eye blinking does not affect BOLD signal changes in the primary visual cortex. This means further that eye blinking can be ignored in data processing.

• Proceedings of the Korea Contents Association Conference
• /
• 2011.05a
• /
• pp.201-202
• /
• 2011

본 연구에는 7.0T 동물용 자기공명영상장치를 이용하여 인간의 췌장암 세포인 CFPAC-1를 이종 이식한 쥐에서 자기공명영상을 획득하여 최적화된 검사 Protocol을 정립하며, 동물 실험에서 밝혀진 종양특성과 확산강조영상과의 비교 분석을 해보고 현성확산계수 영상이 췌장암 이종이식 모형의 종양 세포 내부 구조에 관하여 어떠한 정보를 제공 할 수 있는지 알아보고자 한다. 13마리의 쥐의 26개의 종양을 전형적으로 주입 후에 2~4주 뒤에 직경이 5~10mm가 되었을 때 imaged 하였으며, pathologic specimenm을 위해 sacrificed 하였다. isofluoran gas anesthesia를 이용하여 동물 마취 하였다. 사용된 장비로는 small-animal MR images (7.0-T)를 (Bruker BioSpin GmbH, Rheinstetten, Germany)이용하여 Fast T2-weighted 와 single-shot EPI DW image를 얻었다. 종양은 H&E 염색과 CD31와 VEGF에 대한 면역조직학 염색을 하여 종양의 cellularity와 microvessel density(MVD), 종양 내 괴사 정도를 평가하였다. CFPAC-1의 현성확산계수값은 $0.7327{\pm}0.1075{\pm}10^{-3}mm2/s$이였으며, 현성확산계수는 종양내 괴사 정도와 연관성을 보였다(R = 0.7417, p = 0.0001) 이처럼 현성확산계수는 종양 내 괴사 정도 등의 현미경적구조변화를 반영하는 대리인자로 사용될 수 있음을 확인하였다.

• Proceedings of the Korean Institute of Information and Commucation Sciences Conference
• /
• 1999.05a
• /
• pp.415-419
• /
• 1999

Many bipolar logic circuit of conventional occurred problem of speed delay according to deep saturation state of vertical NPN Transistor. In order to remove minority carries of the base region at changing signal in conventional bipolar logic circuit, we made transistor which is composed of NPN transistor shortened buried layer under the Base region, PNP transistor which is merged in base, epi layer and substrate. Also the Ring-Oscillator for measuring transmission time-delay per gate was designed as well. The structure of Gate consists of the vertical NPN Transistor, substrate and Merged PNP Transistor. In the result, we fount that tarriers which are coming into intrinsic Base from Emitter and the portion of edge are relatively a lot, so those make Base currents a lot and Gain is low with a few of collector currents because of cutting the buried layer of collector of conventional junction area. Merged PNP Transistor's currents are low because Base width is wide and the difference of Emitter's density and Base's density is small. we get amplitude of logic voltage of 200mv, the minimum of transmission delay-time of 211nS, and the minimum of transmission delay-time per gate of 7.26nS in AC characteristic output of Ring-Oscillator connected Gate.

• Proceedings of the KOSOMBE Conference
• /
• v.1985 no.06
• /
• pp.22-25
• /
• 1985

본고에서, "나선형 주사 방법에 의한 고속 NMR 영상화" 방법을 제안하고 그에 따른 실험 결과를 보였다. 이것은 2차원 FID 영역을 나선형 궤적으로 스캐닝하며 데이타를 받을 수 있도록 경사 자계 파형 (gradient pulse)을 가하여 빠른 시간에 (수십 msec - 수초)내에 영상 정보를 얻어낸 후, 재구성 알고리즘을 씀으로써 영상을 얻어내는 방법이다. 이 방법의 장점은 첫째로 $T_2$ 감쇄에 의한 PSF (Point Spread Function)가 윈형 대칭으로 주어지므로 영상화 했을때 물체의 구조 식별이 기존의 EPI (Echo Planar Imaging) 방법에 비해서 선명하며, 둘째로 나선형 궤적을 구현하기 의한 경사 자계 파형에서 불연속 점을 없앰과 동시에 파형의 세기가 점차로 증가하는 형태이므로, 기존의, 파형 왜곡에 의해 영상에 미치는 영향을 최소화 할 수가 있으며, 세째로 나선형 스캔을 사이 사이에 끼워 넣는 방법을 씀으로써 해상도를 향상시킬 수가 있다.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
• /
• v.18 no.10
• /
• pp.877-882
• /
• 2005

In order to fabricate a high breakdown SiC-SBD (Schottky barrier diode), we investigate an effect on metal guard ring (MGR) in breakdown characteristics of the SiC-SBD. The breakdown characteristics of MGR-type SiC-SBD is significantly dependent on both the guard ring metal and the alloying time of guard ring metal. The breakdown characteristics of MGR-type SiC-SBDs are essentially improved as the alloying time of guard ring metal is increased. The SiC-SBD without MGR shows less than 200 V breakdown voltage, while the SiC-SBD with Al MGR shows approximately 700 V breakdown voltage. The improvement in breakdown characteristics is attributed to the field edge termination effect by the MGR, which is similar to an implanted guard ring-type SiC-SBD. There are two breakdown origins in the MGR-type SiC-SBD. One is due to a crystal defects, such as micropipes and stacking faults, in the Epi-layers and the SiC substrate, and occurs at a lower electric field. The other is due to the destruction of guard ring metal, which occurs at a higher electric field. The demolition of guard ring metal is due to the electric field concentration at an edge of Schottky contact metal.

• Journal of Ginseng Research
• /
• v.21 no.2
• /
• pp.69-77
• /
• 1997

In order to explain the connection between ginseng rust spot and soil ecological conditions, the bed soils and ginseng roots were sampled at different microrelief units and the reducing substances of the bed soils and iron forms of the ginseng root epi dermises were determined. The results showed that the occurrence of the rust spot was connected with the ecological conditions of the soils and the metabolism of the plant which was caused by the excessive $Fe^{2+}$ in the soil solution. Ginseng rust spot was the enrichment of iron which was mainly composed of organic complex irons. Including active ferrous active ferric and non active ferric forms and they were transformed into each other following the change of soil moisture and temperature regimes. According to the regularity of growth and decline of reducing substances in soil and rust index of ginseng roots as well as the difference of adaptability to excessive $Fe^{2+}$ in soil among different year-old seeding, a new comprehensive measure based on the connection of ameliorating soil and improving cultivation system was recommended to prevent the occurrence of ginseng rust spot.

• Proceedings of the Materials Research Society of Korea Conference
• /
• 2011.05a
• /
• pp.52.2-52.2
• /
• 2011

Flat form technology for constructing anticancer loaded multi-walled carbon nanotubes (mwCNTs) was introduced in this study. Conventional anticancer drugs, such as MTX (Methotrexate), cisplatin, DOX (Doxorubicin hydrochloride), DAU (Daunorubicin) and EPI (epirubicin) were bio-conjugated with folic acid (FA) for selective targeting tumor cells. Loading efficiencies of the used anticancer drugs on mwCNTs have shown different order of bindings depending on the molecular bind affinity of NH (amine) formation on mwCNTs. MTT assays have shown increased selective target efficiency of FA conjugated mwCNTs on breast cancer cell growth inhibition. All results collectively indicated promising application of mwCNTs as a smart inorganic nanomaterial for selective targeting drug delivery vehicle at tumor tissues.