• Toxicological Research
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• 제24권1호
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• pp.79-86
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• 2008

This study was conducted to obtain information of the oral dose toxicity of low molecular fucoidan (LMF) in male and female mice. In order to calculate 50% lethal dose ($LD_{50}$) and approximate lethal dose (LD), test material was once orally administered to male and female ICR mice at dose levels of 2000, 1000, 500, 250, 125 and 0 (vehicle control) mg/kg (body wt.). The mortality and the changes on body weight, clinical signs, gross observation and organ weight and histopathology of principle organs were monitored 14 days after LMF treatment. We could not find any mortalities, clinical signs, body weight changes and gross findings. In addition, significant changes in the organ weight and histopathology of principal organs were not observed except for some sporadic findings. The results obtained in this study suggest that LMF may not be toxic in mice and may be therefore safe for clinical use. The $LD_{50}$ and approximate LD in mice after single oral dose of LMF were considered over 2000 mg/kg in both female and male mice.

• 융합신호처리학회논문지
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• 제11권1호
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• pp.32-40
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• 2010

확장된 네이보어-제르니케 방법을 이용하여 광학계의 동공함수(pupil function)를 추산하는 알고리즘을 제안한다. 동공함수로부터 광학계가 가진 파면수차를 복원하고 아울러 동공에 비추어지는 빛의 밝기분포를 분석할 수 있다. 이 방법은 브루-레이 광픽업의 생산현장에 적용하여 실시간으로 광학계의 수차를 복원할 수 있다. 아울러 이 방법이 여러 다른 광학계에 널리 이용될 수 있음을 다양하게 수차를 부여해서 생성한 데이터를 분석하여 검증하였다. 초점영역의 빔 데이터로부터 동공함수를 얻는 역변환의 여러 방법 중에서 수차이론에 근거한 기존의 네이보어-제르니케 방법을 최근에 네덜란드 연구진이 개량한 확장된 네이보어-제르니케(extended Nijboer-Zernike: ENZ) 방법을 제안하였다. ENZ 방법은 초점영역의 3차원 빔 데이터로부터 해석적인 접근을 통하여 동공의 제르니케 계수를 효율적으로 계산해 낸다. 그러나 이 방법에서는 개구수(NA)가 큰 경우에는 근사적인 접근을 하도록 되어 있다. 본 연구에서는 보다 일반적인 상황에서 적용할 수 있는 수치해석 알고리즘을 개발하고, 이를 GUI 환경에서 운용할 수 있는 윈도 기반의 프로그램을 작성하여 생성한 빔 데이터에 적용하여 이 알고리즘의 유효성을 검증하여 13개의 복소 수차인자 $\beta_n^m$이 모든 요소가 0.7까지 분석을 하였고, 아울러 광픽업의 3D 빔 데이터를 실측해서 간섭계로 측정한 데이터와 R=0.98 정도의 상관관계를 가지는 것을 확인하였다.

• 한국동물학회:학술대회논문집
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• 한국동물학회 2001년도 공동 춘계학술대회
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• pp.82.1-82
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• 2001

No Abstract, See Full Text

• Toxicological Research
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• 제23권1호
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• pp.65-72
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• 2007

This study was conducted to obtain information of the oral dose acute toxicity of PGB-2, a novel polyglucosamine polymer produced from Citrobacter sp. BL-4 (a new strain) in male and female mice. Mortality, body weight changes, clinical signs were monitored during 14 days after single oral dose of test article at dose levels of 2000, 1000, 500, 250 and 125 ml/kg. Gross lesions, organ weight and histopathology of principal organs were examined after necropsy. As the results, we could not find any mortalities, clinical signs, changes in the body weight and gross findings except for white foci in the liver. In addition, no PGB-2-treatment related abnormal changes on the organ weight and histopathology of principle organs were detected except for atypical signs of liver. White liver foci were confirmed as focal infiltration of inflammatory cells. The results suggest that the PGB-2 is relatively safe in mice but the possibility of hepatotoxicity could not be excluded. The $LD_{50}$ and approximate LD in mice after single oral dose of PGB-2 were considered over 2000 mg/kg, respectively. In future, the potential hepatotoxicity of PGB-2 should be evaluated through the repeat dose toxicity test prior to develop as a new agent.

• Toxicological Research
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• 제23권4호
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• pp.373-382
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• 2007

This study was conducted to obtain acute information of the oral dose toxicity of PGB-1, a novel polyglucosamine polymer produced from a new strain Enterobacter sp. BL-2 in male and female mice. In order to calculated 50% lethal dose ($LD_{50}$) and approximate lethal dose (LD), test material was once orally administered to male and female ICR mice at dose levels of 2000, 1000, 500, 250, 125 and 0 (vehicle control) ml/kg (body wt.). The mortality and changes on body weight, clinical signs, gross observation and organ weight and histopathology of principle organs were monitored 14 days after dosing with PGB-1. We could not find any mortalities, clinical signs, body weight changes and gross findings. In addition, significant changes in the organ weight and histopathology of principal organs were not observed except for some sporadic findings. The results obtained in this study suggest that PGB-1 may not be toxic in mice and may be therefore safe for clinical use. The $LD_{50}$ and approximate LD in mice after single oral dose of PGB-1 were considered over 2000 mg/kg in both female and male mice.

• Biotechnology and Bioprocess Engineering:BBE
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• 제5권6호
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• pp.465-468
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• 2000

Methyl mercaptan oxidase was successfully induced from Rhodococcus rhodochrous IGTS8 using methyl mercaptan gas and purified to homogeneity for the detection of mercaptans. The purification procedure involved DEAE-Sephacel and Superose 12 column chromatography with recovery yields of 85.8 and 83.3%, and a specific activity of 92.7 and 303.4 units/mg-protein, respectively. The molecular weight of purified methyl mercaptan oxidase was determined to be 64.5 kDa by SDS-PAGE. The extract from gel filtration chromatography oxidizes methyl mercaptan to produce formaldehyde, which can be easily detected by the purpald-coloring method. Optimum temperature for activity was achieved at 60$^{\circ}C$. This enzyme was inhibited by both K$_2$SO$_4$and NaCl at concentration of less than 100mM and recovered to original activity at concentration of 200mM. In the presence of methanol, the activity decreased by 33%.

• Journal of Microbiology and Biotechnology
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• 제13권4호
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• pp.628-631
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• 2003

The recombinant alanine dehydrogenase (ADH) from E. coli containing Thermus caldophilus ADH was purified to homogeneity from a cell-free extract. The enzyme was purified 38-fold with a yield of 68% from the starting cell-free extract. The purified enzyme gave a single band in polyacrylamide gel electrophoresis, and its molecular weight was estimated to be 45 kDa. The pH optimum was 8.0 for reductive amination of pyruvate and 12.0 for oxidative deamination of L-alanine. The enzyme was stable up to $70^{\circ}C$. The activity of the enzyme was inhibited by 1 mM $Zn^{2+}$, 20% hexane, and 20% $CHCl_3$. However, 10 mM $Mg^{2+}$ and 40% propanol had no effect on the enzyme activity. The Michaelis constants ($K_m$) for the substrates were $50\;\mu\textrm{M}$ for NADH, 0.2 mM for pyruvate, 39.4 mM for $NH_4+$, 2.6 mM for L-alanine, and 1.8 mM for $NAD^+$.

• 한국수자원학회논문집
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• 제44권1호
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• pp.9-22
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• 2011

본 연구에서는 에너지 방정식에 기초하여 정수 식생이 존재하는 자연하도에 적용 가능한 1차원 수치모형을 제시하였다. 수위계산을 위한 마찰경사는 Darcy-Weisbach의 마찰식에 의해 계산되었다. 각 단면의 전체 Darcy-Weisbach 마찰계수는 하상조도높이, 식생, 식생구역과 비식생구역 사이의 전단저항, 그리고 홍수터와 주수로의 경계면 전단저항을 고려하여 산정하였다. 경계면 마찰계수는 Mertens방법과 Nuding방법에 의해 계산되었다. 제시된 모형을 가상하도에 적용하고 HEC-RAS모형 모의결과와 비교, 검증하였고, 식생밀도 등에 대하여 마찰계수의 민감도 분석을 실시하였다. 모형의 적용성검토를 위하여 독일 Enz강 일부구간을 대상으로 수치모의를 실시하고 실측수위와 비교하였다. 식생이 존재하는 Enz강의 실측수위와 계산수위를 비교한 결과 식생의 영향을 거의 받지 않고 주수로에 흐름이 형성되는 저유량의 경우와 식생의 영향을 지배적으로 받는 고유량 흐름 모두에서 합리적인 수위를 예측하여 제시된 모형의 적용 가능성을 확인하였다.

• Asian-Australasian Journal of Animal Sciences
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• 제21권10호
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• pp.1425-1434
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• 2008

The study was conducted to evaluate and compare the effects of feed additives on ruminal fermentation, nutrient digestibility and performance of lambs fed diets containing 60% wet brewers grains (WBG). In Experiment 1, two simultaneous trials were conducted. Fifty intact ($20.2{\pm}0.8kg\;BW$) lambs were used in a feedlot trial and 10 (rumen cannulated; $32{\pm}1kg\;BW$) in a digestion trial. The pH, volatile fatty acids (VFA) and ammonia-N in lambs were also estimated. Lambs were randomly assigned to one of five diets: i) without additives (Con), ii) with 1% bicarbonate (Bic), iii) with 1% bentonite (Ben), iv) with 33 mg/kg monensin (Mon) and v) with 200 mg/kg fibrolityc enzymes (Enz). In Experiment 2, 120 RambouilletPelibuey intact male lambs ($19.5{\pm}1.5kg\;BW$) were used in a feedlot trial and randomly assigned to four diets: i) without additives (control), ii) with 1% Bic, iii) with 33 mg/kg Mon and iv) with 1% Bic and 33 mg/kg Mon. In Experiment 1, lambs fed diets containing Bic or Mon had significantly higher final weight, DMI, ADG than other lambs. However, apparent DM, OM, CP, NDF and ADF digestibilities and ruminal individual VFA content were similar (p>0.05) among treatments. Conversely, treatmentcollection period interaction was significant for ruminal pH and NH3. In Experiment 2, lambs fed diets containing a Bic and Mon combination had significantly higher final weight, DMI and ADG. It is concluded lambs fed Bic or Mon or Bic and Mon combination had better performance characteristics than lambs on Ben or Enz.

• Toxicological Research
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• 제21권4호
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• pp.361-365
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• 2005

This study was conducted to obtain the acute information of the oral dose toxicity of Polycan - originated from Aureobasidium pullulans SM-2001 (half of the dry material is -1,3/1,6-glucans), a UV induced mutant of A. pullulans, having various pharmacological effects, in male and female mice. In order to calculate $50\%$ lethal dose $(LD_{50})$, approximate LD and target organs, test article was administered twice by oral gavage to male and female ICR mice at total 1000, 500 and 250mg/kg. The mortality and changes on body weight, clinical signs and gross observation were monitored during 14 days after dosing. As the results, we could not find any mortalities, clinical signs, changes in the body weight and gross findings. The results obtained in this study suggest that the Polycan is non-toxic in mice and is therefore likely to be safe for clinical use. The L050 and approximate $(LD_{50})$ in mice after single oral dose of Polycan were considered over 1000 mg/kg, respectively.