• Journal of Life Science
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• v.28 no.6
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• pp.648-655
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• 2018

Enhancement mechanistic actions of testosterone (TS) productions in mouse Leydig TM3 cells by the eritadenine (EA) and/or the Agaricus blazei mycelial liquid culture extract (ABMLCE). Productions of TS in TM3 cells were investigated in normal and oxidative-stressed culture conditions. In the normal culture condition, TM3 cells grown in a Dulbecco's Modified Eagle's Medium (DMEM) were treated with EA (0~100 ppm) and ABMLCE (10 ppm) + EA (0~50 ppm) for 24 hr, and in the oxidative-stressed culture condition, the cells grown in DMEM containing $50{\mu}M$ $H_2O_2$ to induce oxidative stress for 4 h were treated with the same as those in the normal culture condition. TS content, $3{\beta}$-hydroxysteroid dehydrogenase 2 (HSD3B2) enzyme activity, $5{\alpha}$-reductase 2 ($5{\alpha}-R2$) enzyme activity, and free-radical nitric oxide (NO) content in the culture media were measured using their corresponding assay kits. EA, ABMLCE, and ABMLCE + EA significantly, p<0.05, enhanced TS productions in both cultural conditions, relative to control treatment. The activity of the HSD3B2 enzyme, which is involved in the production of precursors for TS production, was elevated by EA, ABMLCE, and ABMLCE + EA treatments in both culture conditions. The activity of the $5{\alpha}-R2$ enzyme, which converts TS to dihydroxytestosterone (DHT), was not significantly affected in either culture condition by EA, ABMLCE, or ABMLCE + EA treatments. The treatments included reduced NO content. These results indicate that EA, ABMLCE, and EA + ABMLCE treatments elevated TS in TM3 cells via the enhancements of HSD3B2 activity and the reduction of NO production, and also imply that EA and ABMLCE or EA + ABMLCE could be useful materials for the production of TS in humans.

• Journal of the Korean Institute of Landscape Architecture
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• v.48 no.5
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• pp.59-66
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• 2020

Recently, cultivation and management technologies have been needed to adapt due to climate change, which is causing abnormal weather conditions. One technique is to increase the utilization of evergreen broad-leaved species with high ornamental value. A total of five treatments were installed (1m×22.5m), including 60g/㎡ and 80g/㎡ using two types mulching material with an overlapping and hole-drilling mulching method and these were compared to un-mulching treatment a total of planted 92㎡ attheWol-aTestSiteForestattheForestforBiomaterialsResearchCenterinJinju-si, Gyeongsangnam-dofor 10monthsusing3-years-oldQuercusglaucaThunb. In comparison with the control site, the 60g/㎡ overlapping method was about 1.9 times higher than the root collar diameter, but there was no statistical significance between the treatments. Healthy seedlings were found to meet these conditions due to high biomass values and below and T/R ratios of 3.0 or lower and H/D ratios of 7.0 or lower. Comparing the values of LWR, SWR, and RWR, which can be evaluated for seedling due to the mulching treatments, as compared to the control, the growth of the ground areas including leaves and stems was enhanced, but the growth of the underground areas containing roots tended to have high control values. Based on this, the SQI value, which can be evaluated for the comprehensive quality of seedlings, was found to be significantly different between the control site and the mulching treatment sites, confirming that the growth and growth improvement effects were achieved with mulching treatments. The chlorophyll content analysis showed that there was a significant difference from the control site, and it was judged that weed generation in the control acted as an environmental stress, causing a decrease in chlorophyll content. It was found that the overlapping 80g/㎡ of polypropylene mulching material generated about 4 times fewer weeds than the control, and the manpower required for the mulching test field and weeding were equal at 3.3 people/100㎡/1 day. Mulching treatments have demonstrated a significant difference in the promotion of growth and quality of the seedlings and are judged as an alternative that can reduce the economic burden incurred by the purchase of the supplies and the manpower required to weed forestry plantations.

• Journal of Bio-Environment Control
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• v.20 no.2
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• pp.93-100
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• 2011

Maintenance of adequate soil water potential during the period of crop growth is necessary to support optimum plant growth and yields. A better understanding of soil water movement within and below the rooting zone can facilitate optimal irrigation scheduling aimed at minimizing the adverse effects of water stress on crop growth and development and the leaching of water below the root zone which can have adverse environmental effects. The objective of this study was to evaluate the feasibility of using a portable irrigation controller with an Watermark sensor for the cultivation of drip-irrigated vegetable crops in a greenhouse. The control capability of the irrigation controller for a soil water potential of -20 kPa was evaluated under summer conditions by cultivating 45-day-old tomato plants grown in three differently textured soils (sandy loam, loam, and loamy sands). Water contents through each soil profile were continuously monitored using three Sentek probes, each consisting of three capacitance sensors at 10, 20, and 30 cm depths. Even though a repeatable cycling of soil water potential occurred for the potential treatment, the lower limit of the Watermark (about 0 kPa) obtained in this study presented a limitation of using the Watermark sensor for optimal irrigation of tomato plants where -20 kPa was used as a point for triggering irrigations. This problem might be related to the slow response time and inadequate soil-sensor interface of the Watermark sensor as compared to a porous and ceramic cup-based tensiometer with a sensitive pressure transducer. In addition, the irrigation time of 50 to 60 min at each of the irrigation operation gave a rapid drop of the potential to zero, resulting in over irrigation of tomatoes. There were differences in water content among the three different soil types under the variable rate irrigation, showing a range of water contents of 16 to 24%, 17 to 28%, and 24 to 32% for loamy sand, sandy loam, and loam soils, respectively. The greatest rate increase in water content was observed in the top of 10 cm depth of sandy loam soil within almost 60 min from the start of irrigation.

• Horticultural Science & Technology
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• v.28 no.4
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• pp.529-534
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• 2010

Occurrence of wilted symptom in watermelon plant ($Citrullus$ $lanatus$ L.) is known to be caused by physiological disorder. The symptom results in the loss of fruit production and thus the economical loss of watermelon growers. The incidence of symptom is often found from the middle of March to the end of May in the major watermelon crop production areas of Korea (i.e. Uiryeong, Gyeongnam (lat $37^{\circ}$56'64"N, long $126^{\circ}$99'97"E)). Despite of extensive information about the physiological disorder, little study has been conducted to understand a relationship between the wilted symptom and accompanying environment factors (e.g. temperature). This study aimed to investigate effects of environmental conditions amended by a forced-ventilation system on physiological characteristics of watermelon and incidence of the wilted symptom. Watermelon plants were grown from January to May, 2009 with either the forced-or natural-ventilation treatment in a greenhouse located in the Uiryeong. In the result, the forced-ventilation treatment decreased the air, leaf and root-zone temperature approximately $4.5^{\circ}C$, $5^{\circ}C$ and $3^{\circ}C$, respectively, compared to the natural-ventilation. The fruit growth rate was maximized twice during the entire growing period. The higher rate of fruit growth was observed under the natural-ventilation than the forced one. Maximization of the fruit growth rate (approximately 430 g per day) was first observed by 12 days after fruiting under the natural-ventilation treatment, while the second one (approximately 350 g per day) was observed by 24 days after fruiting. The wilted symptom started occurring by 22 days after fruiting under the natural-ventilation, whereas no incidence of the symptom was found under the forced-ventilation treatment. Interestingly, the forced-ventilation lowered the fruit growth rate (approximately 320 g per day) compared to the natural one. Maximization of the fruit growth rate under the forced-ventilation was found at 4 days later than that under the natural one. This result coincided with a slower plant growth under the forced-ventilation treatment. These results suggest that the forced-ventilation slows down extension growth of fruit and plant, which may be associated with lowering leaf temperature and saturation deficit. We suggest the hypothesis that the forced-ventilation may alleviate stress of the wilted symptom by avoiding extreme water evaporation from leaves due to high temperature and thus by reducing competition between leaves and fruits for water. More direct and detailed investigations are needed to confirm the effect of the forced ventilation.

• Journal of Korea Technical Association of The Pulp and Paper Industry
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• v.48 no.2
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• pp.34-45
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• 2016

Global warming and climate change have been caused by combustion of fossil fuels. The greenhouse gases contributed to the rise of temperature between $0.6^{\circ}C$ and $0.9^{\circ}C$ over the past century. Presently, fossil fuels account for about 88% of the commercial energy sources used. In developing countries, fossil fuels are a very attractive energy source because they are available and relatively inexpensive. The environmental problems with fossil fuels have been aggravating stress from already existing factors including acid deposition, urban air pollution, and climate change. In order to control greenhouse gas emissions, particularly CO2, fossil fuels must be replaced by eco-friendly fuels such as biomass. The use of renewable energy sources is becoming increasingly necessary. The biomass resources are the most common form of renewable energy. The conversion of biomass into energy can be achieved in a number of ways. The most common form of converted biomass is pellet fuels as biofuels made from compressed organic matter or biomass. Pellets from lignocellulosic biomass has compared to conventional fuels with a relatively low bulk and energy density and a low degree of homogeneity. Thermal pretreatment technology like torrefaction is applied to improve fuel efficiency of lignocellulosic biomass, i.e., less moisture and oxygen in the product, preferrable grinding properties, storage properties, etc.. During torrefacton, lignocelluosic biomass such as palm kernell shell (PKS) and empty fruit bunch (EFB) was roasted under an oxygen-depleted enviroment at temperature between 200 and $300^{\circ}C$. Low degree of thermal treatment led to the removal of moisture and low molecular volatile matters with low O/C and H/C elemental ratios. The mechanical characteristics of torrefied biomass have also been altered to a brittle and partly hydrophobic materials. Unfortunately, it was much harder to form pellets from torrefied PKS and EFB due to thermal degradation of lignin as a natural binder during torrefaction compared to non-torrefied ones. For easy pelletization of biomass with torrefaction, pellets from PKS and EFB were manufactured before torrefaction, and thereafter they were torrefied at different temperature. Even after torrefaction of pellets from PKS and EFB, their appearance was well preserved with better fuel efficiency than non-torrefied ones. The physical properties of the torrefied pellets largely depended on the torrefaction condition such as reaction time and reaction temperature. Temperature over $250^{\circ}C$ during torrefaction gave a significant impact on the fuel properties of the pellets. In particular, torrefied EFB pellets displayed much faster development of the fuel properties than did torrefied PKS pellets. During torrefaction, extensive carbonization with the increase of fixed carbons, the behavior of thermal degradation of torrefied biomass became significantly different according to the increase of torrefaction temperature. In conclusion, pelletization of PKS and EFB before torrefaction made it much easier to proceed with torrefaction of pellets from PKS and EFB, leading to excellent eco-friendly fuels.

• Journal of Animal Science and Technology
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• v.45 no.4
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• pp.649-658
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• 2003

Early introducing the ducks into the paddy field involves a variety of environmental changes such as new surroundings and contact with water, so that some free-ranged ducks show behavioral and physiological changes indicative of stress or die from not adapted for new circumstances. Moreover, the free-ranged ducks was tread on the rice plant, and required a great deal of labor and time for captured the ducks after finishing the introducing. The aim of the present study was to examine the effect of accustoming and imprinting of duckling on behavior patterns, body weight gain and yield of the rice plant in paddy fields. Three paddy plots were used as control (no imprinting and no contact with people), taming (imprinting and regular handling) and roughness (handling roughly and strike terror to ducks) plots. Right after introducing the ducks into the paddy field, eating and moving behaviour of taming plot ducks tended to be longer time spend than that of other treatments. However, eating and moving time tended to be longer in the control than that of other two treatments on the 2 weeks after. Flighting distance was not showed in the free-ranging period taming plot, but the control plot was significantly (P<0.05) longer than the roughness plot. The captured time of free-ranged ducks tended to be shorter in the order of taming, control and roughness plots. Body weight gains was not significant. The number of rice plants damaged by free-ranging ducks in the taming plot were significantly (P<0.05) less than that in the control plot, but the yield and yield components of the rice plant were not differ among 3 treatments. These results indicated that the imprinting or regular handling and related treatments of duckling was reducing badly damage of rice plants, captured time and labor of free-ranged ducks in paddy field, although the working behavior of ducks and yield ability of the rice plants were not affected.

• Korean Journal of Soil Science and Fertilizer
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• v.9 no.3
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• pp.165-181
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• 1976

Fertilizer(N,P,K) demand for crop production in 1980, 1990 and 2000 was estimated according to the two proposed models, one of which is fertilizer use efficiency model expressed in $Fn=(Y/E){\cdot}(1-Cs)Eu$, where Fn:fertilizer demand, Y:Crop production estimated, E:nutrient efficiency, Cs:fraction of natural resource nutrient in plant, Eu:fertilizer use efficiency and the other fertilization efficiency model expressed in Fn=Y(1-Cys)/Fe, where Cys:fraction of yield without fertilizer, Fe:fertilization efficiency. Total crop uptake of nutrient and its noncycling portion were estimated as criteria for fertilizer demand and nutrient maintenance. Total crop uptake of N,P,K was 600,000 M/T in 1965 700,000 M/T in 1974 and estimated to 880,000 M/T in 1980, 1,170,000 M/T in 1990 and 1,410,000 M/T in 2000. Fertilizer demand appeares to be about 90% of total crop uptake according to fertilizer use efficiency model and about 87% according to fertilization efficiency model. The noncycling nutrient was about 29% of total crop uptake. Fertilizer demand was almost same to the uptake amount in nitrogen, 1.5 times of uptake in phosphorus and half of uptake in potassium. Varietal development, improvement of soil fertility and cultivation method and development of fertilizer forms appears to decrease fertilizer demand by increasing efficiency term in two models while environmental stress such as low temperature appears to give reverse effect resulting in higher fertilizer demand. Fertilizer consumption in 1974 seemed to be unreasonably high especially in nitrogen and phosphorus and thus the effective use of fertilizer appeared as an urgent problem considering that large fields are still remained in lower fertility.

• Radiation Oncology Journal
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• v.17 no.1
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• pp.70-77
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• 1999

Purpose : The expression of p53, P211WAF/CIP, Bcl-2, and Bax underlying the radiation-induced apoptosis in different pH environments using SCK mammary adenocarcinoma cell line was investigated. Materials and Methods Mammary adenocarcinoma cells of hi) mice (SCK cells) in exponential growth phase were irradiated with a linear accelerator at room temperature. The cells were irradiated with 12 Gy and one hour later, the media was replaced with fresh media at a different pHs. After Incubation at 37Microbioiogy, College of Medicine Dong A University for 0$\~$48 h, the extort of apoptosis was determined using agarose gel electrophoresis and flow cytometry. The progression of cells through the cell cycle after irradiation in different pHs was also determined with flow cytometry. Western blot analysis was used to monitor p53, p211WAFfCIP, Bcl-2, and Bu protein levels. Results : The induction of apoptosis by irradiation in pH 6.6 medium was markedly less than that in pH 7.5 medium. The radiation-induced G2IM arrest in pH 6.6 medium lasted markedly longer than that in pH 7.5 medium. Considerable amounts of p53 and p21 proteins already existed at pH 7.5 and increased the level of p53 and p21 significantly after 12 Gy X-irradiation. An incubation at pH 6.6 after 12 Gy X-irradiation did not change the level of p53 and p21 protein levels significantly. Bcl-2 proteins were not significantly affected by radiation and showed no correlation with cell susceptibility to radiation-induced apoptosis in different pHs. An exposure to 12 Gy of X-rays increased the level of Bax protein at pH 7.5 but at pH 6.6, it was slight. Conclusions : The molecular mechanism underlying radiation-induced apoptosis in dinerent pH environments using SCK mammary adenocarcinoma cell line was dependent of the expression p53 and P211YVAF/CIP proteins. We may propose following hypothesis that an acidic stress augments the radiation-induced G2iM arrest, which inhibiting the irradiated cells undergo post-mitotic apoptosis. The effects of environmental acidity on anti-apoptotic and pro-apoptotic function of Bcl-2 family was unclear in SCK mammary adenocarcinoma cell line.

• Journal of Korean Academy of Nursing
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• v.4 no.1
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• pp.1-21
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• 1974

A considerable change of the Korean nursing system has been made during the last decade not only in its philosophy but also in its function and structure to meet political and scientific need of the modern society. The main purpose of this study is to develope the new concept of comprehensive nursing care, both its Philosophy and ethics, as the basic of modern clinical nursing theory and practice. Comprehensive nursing care is the concept of human centered nursing care, and it helps a man to meet the basic physical, psychological, environmental, socioeconomic and teaching needs. It also helps him to help himself to meet these needs. This concept starts from the individualized nursing care and its ultimate goal is to improve a man to have a better position in his own community so that he may able to have a meaningful life. To accomplish this goal, an individualized nursing care plan as a nursing diagnosis and problem solving method should be set up for different patients with similar diagnosis to meet their needs, because each patient has a different social background. from this viewpoint, nursing is a science as well as abstruse humanity. The performance of comprehensive nursing care is a goal and issue of modern clinical nursing care. If nursing is a science and a profession for man, it should have ethics which recognize the dignity of man and offers infinite service voluntarily, and should be able to show leadership in carrying out the nursing responsibility. This leadership finds a person's potential and encourages him to utilize it. Such concepts should develop into a nursing ideology and this ideology should become a priority in comprehensive nursing care. The following statements are the conclusion of this study. 1) Modern nursing has been developed from disease centered nursing care to comprehensive nursing care based on humanity. The primary principle of nursing was to assist in the treatment of disease, but it has been changed to the professional nursing system independently. 2) The concept of nursing is one of continuous or endless scope of dispersion. It proves that nursing is grasping the professional responsibility to be able to coordinate scientific principles Patient health problems are according to scientific principles rather than adhering to nursing technical discipline as a daily work. 3) In chapter I and Ⅱ, the philosophy and ideology of nursing have been discussed and the flow of concept of clinical nursing and the rate of progress which emerges from naturalizing performance of the concept of comprehensive nursing in clinical nursing studied. The discussion developed the theory that a nurse should be to embody nursing ideas and objectives by establishing definite conviction of professions and study. 4) In chapter lil, nursing planning based on nursing diagnosis as a method to attain ideal nursing care for humanity with a definite idea of establishing philosophy of nursing was presented. 5) From the result of survey on patient needs about treatment and nursing, it was observed that all patient had emotional stress from unknown factors. Therefore it was concluded that nurses should not only educate the patient but also give them the opportunity to communicate freely their needs and anxieties. Furthermore complaints and doubts of the patient should be carefully noted and must be considered to meet these needs. 6) Patient teaching is the most important part of comprehensive nursing care. In chapter, Ⅲ, the important of patient teaching was emphasized by demonstrating the effect of patient teaching for diabetic patient. 7) In Chapter Ⅳ, from the result of the study on nurses attitudes to comprehensive nursing care, it was pointed that the evolution of nursing education and the establishment of a complete concept and value of comprehensive nursing was necessary.

• KSCE Journal of Civil and Environmental Engineering Research
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• v.4 no.2
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• pp.133-142
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• 1984

With the weld-joined compact tension specimens compared with each other, that is, transverse and lengthwise about the crack propagation direction, high and low in the input heat level, same as and lower than the base metal in the strength of weld material, the fatigue test were performed. With these data, the log-log curves between the fatigue crack propagation rate ${\frac{da}{dN}}$ and the transition range of the stress intensity factor ${\Delta}K$ ahead the crack tip were drawed. These curves were compared and estimated among each compared specimens, among each zones, that is, the base metal, the heat-affected metal and the weld-mixed metal, and between this study and the past studies. Basically, Little difference in the slope of the $da/dN-{\Delta}K$ relation was showed in all the welded directions, all the input heat levels and all the zones. But, First, to comparison with in the past studies about the base metals, it was showed that da/dN started in the much later rate, increased faster and stoped in the little faster rate. Second, it was showed that, near the time the crack's going from the heat-affected zone to the weld-mixed metal da/dN decreased a little for a while. Third, in the lengthwise weld compared with the transverse weld, in the high input heat weld compared with the low input heat weld in the case used the weld material of the same strength as the base metal, in the opposite case in the case used the one of the lower strength than the base metal, in the case used the weld material of the same strength as compared with the lower strength than the base metal beside the high input heat and the lengthwise weld, it was showed that the crack occured earlier in lower ${\Delta}K$ and later da/dN, the curves went with the same slope. Forth, in the lengthwise weld compared with the transverse weld in the low input heat weld, in the low input heat weld compared with the high input heat weld, it was showed that da/dN went with the lower level.