• Journal of Acupuncture Research
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• v.21 no.5
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• pp.219-226
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• 2004

Objectives : The purpose of this study is to report that ENoG may be used for concluding prognosis of peripheral facial palsy. Methods : The House-Brackmann facial grading scale of face was taken at onset and 3weeks after oriental medical treatment. The ENoG of face was taken at 1week after or before of onset. I compared the House-Brackmann facial grading scale with ENoG. Results Conclusions : I analyzed a relation of House-Brackmann facial grading scale and ENoG. I guess that ENoG may be used for concluding prognosis of peripheral facial palsy. But further studies are requred for concluding prognosis of peripheral facial palsy by ENoG.

• Journal of Life Science
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• v.9 no.2
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• pp.153-159
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• 1999

To express constitutively the inulinase gene (INUl) of Kluyveromyces marxianus in Saccharomyces cerevisiae, three yeast promoters such as GAPDH, ADH1 and ENO1 were connected upstream of INUl. The resulting plasmids, pYIGP, pADHl-INU, and pENO-INU were introduced to S. cerevisiae SEY2102 host strain, respectively, and then each transformants were selected by staining of colonies on sucrose-agar plate. When the yeast transformants were cultivated on 2$\%$ dextrose media, the total expression levels of inulinase reached to 1.11 unit/mL, 0.88 unit/mL, and 0.69 unit/mL for respective GAPDH, ADH1, and ENO1 promoter systems. On 4% dextrose media, however, the inulinase activities were observed at 2.00 unit/mL for pYIGP, 0.71 unit/mL for pADH1-INU, and 1.40 unit/mL for pENO-INU. This result indicates that the constitutive expression of INUl was significantly affected by the initial concentration of dextrose and the promoter strength was in the order GAPDH, ENO1, and ADH1 promoter at high dextrose concentration. Taking into account the plasmid stability, however, it is suggested that the ENO1 promoter system is more suitable for the INU1 expression on high dextrose medium or in the fed-batch cultivation accumulating ethanol at high level.

• Journal of Korea Water Resources Association
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• v.34 no.1
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• pp.45-55
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• 2001

Transcritical flow is a term intended to denote the existence of both supercritical and subcritical flows within a computational domain. The major problems that need to be addressed while modeling transcritical flows include handling the differing features of signal propagation in subcritical and supercritical flow regions and maintaining conservation. The present study proposes the implicit ENO method as a high-resolution scheme for transcritical flow. This implicit ENO scheme is based on the ENO method, a new class of uniformly high-order-accurate essentially non-oscillatory implicit scheme, which has the advantage of unconditional stability. The implicit ENO scheme has not been used for the transcritical flow in open channel until now. As a result of application to the hypothetical dam-break flow, the implicit ENO scheme was ploved to produce accurate results with good robustness even though in the case of verb strong shock wave.

• Molecules and Cells
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• v.42 no.11
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• pp.804-809
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• 2019

Oncogenic gain-of-function mutations are clinical biomarkers for most targeted therapies, as well as represent direct targets for drug treatment. Although loss-of-function mutations involving the tumor suppressor gene, STK11 (LKB1) are important in lung cancer progression, STK11 is not the direct target for anticancer agents. We attempted to identify cancer transcriptome signatures associated with STK11 loss-of-function mutations. Several new sensitive and specific gene expression markers (ENO3, TTC39C, LGALS3, and MAML2) were identified using two orthogonal measures, i.e., fold change and odds ratio analyses of transcriptome data from cell lines and tissue samples. Among the markers identified, the ENO3 gene over-expression was found to be the direct consequence of STK11 loss-of-function. Furthermore, the knockdown of ENO3 expression exhibited selective anticancer effect in STK11 mutant cells compared with STK11 wild type (or recovered) cells. These findings suggest that ENO3-based targeted therapy might be promising for patients with lung cancer harboring STK11 mutations.

• Bulletin of the Korean Chemical Society
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• v.21 no.9
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• pp.849-854
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• 2000

The protonation and divalent cation complexation equilibria of several quinolone antibiotics such as nalidixic acid (NAL),flumequine (FLU), oxolinic acid (OXO), ofloxacin (OFL), norfloxacin (NOR) and enoxacin (ENO) have been examined by potentiome tric titration and spectrophotometric method. The antibacterial activity of these drugs depends upon the pH and the concentration of metal cations such as Mg2+ , Ca2+ in solu-tion. The apparent ionization constants of NAL, FLU, OXO, OFL, NOR and ENO in aqueous solution were found to be 6.33, 6.51, 6.72, 7.18, 7.26, and 7.53, respectively. In aqueous solution, NAL, FLU and OXO were found to be present mainly as two chemical species : molecularand anionic; but OFL, NOR and ENO were present mainly as three chemical species : anionic, neutral zwitterionic and cationic form, in equilibrium. The pKa1 and pKa2are found to be 6.10 and 8.28 for OFL; 6.23 and 8.55 for NOR; 6.32 and8.62 for ENO, respec-tively. The complex formation constants between OFL, NOR or FLU and some divalent cations are measured at pH 7.5. The 1 : 1 complexes are formed mainly by ion-dipole interaction. FLU has somewhat larger Kf values than OFL and NOR because its molecular size is small and the FLU is present asanionic form at pH 7.5.

• Journal of Korea Water Resources Association
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• v.36 no.1
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• pp.1-11
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• 2003

This paper describes a finite volume, two-dimensional model. It adopts a recently developed essentially non-oscillatory(ENO) schemes based on the Lax-Friedrichs solver, which was modified for a finite volume grid, and employs a modified MUSCL(Monotonic Upstream centered Scheme for Conservation Law) for second-order accuracy in space. To demonstrate the applications of the model, it is applied to solve the 1-D and 2-D dam-break problems. The model in conjunction with the modified MUSCL showed a better agreement with analytical solutions than the minmod function in 1-D dam-break problems and is satisfactorily validated with documented published data in 2-D dam-break problems. The model was applied to tidal wane entering channel at one end, and the results showed a good agreement with analytical solutions. In the channel with reflective boundary conditions specified at the extremities, the model was capable of accurately simulating the wave propagation.

• IMMUNE NETWORK
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• v.11 no.3
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• pp.175-181
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• 2011

Background: CM1 (centrocyte/-blast marker 1) was defined by a mAb against concavabalin-A (ConA) activated PBMC. It is expressed in germinal center of human tonsil and on the surface of activated PBMC as well as cancer cells. Recently, increased productions of pro-inflammatory mediators were detected from activated PBMC by CM1 ligation. Methods: However, there is a limitation to explain the exact role of CM1 on inflammation and its related mechanisms, since the identity of CM1 is still not clarified. In our previous study, we have already confirmed that soluble form of CM1 was produced by Raji. Therefore, we performed Q-TOF analysis after immunoprecipitation of concentrated Raji culture supernatant using anti-CM1 mAbs. Results: As a result, we found that CM1 is identical to enolase-1(ENO1), a glycolytic enzyme, and we confirmed that results by silencing ENO1 using siRNA. It was also confirmed through competition assay between anti-CM1 and anti-ENO1 mAbs. Finally, we investigated the possible role of CM1 in inflammatory response and cancer. The ligation of CM1 on Raji cells with anti-CM1 mAbs induces the extensive production of prostaglandin $E_2(PGE_2)$. In addition, the increased activity of matrix metalloproteinase (MMP)-2/9 was shown in NCI-N87, stomach cancer cell line by CM1 stimulation. Conclusion: CM1 is identical to ENO1 and it might be an important role in the regulation of inflammatory responses.

• Communications of the Korean Mathematical Society
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• v.22 no.4
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• pp.609-621
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• 2007

Comparison of high Mach number jets using positive schemes and Weighted ENO methods is considered in this paper. The positive scheme introduced by [11, 14] and Weighted ENO [9, 10] have allowed us to simulate very high Mach numbers more than Mach 80. Simulations at high Mach numbers and with radiative cooling are essential for achieving detailed agreement with astrophysical images.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.35 no.2
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• pp.94-100
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• 2007

An axisymmetric supersonic jet screech in the Mach number range from 1.07 to 1.2 is numerically simulated. The axisymmetric mode is the dominant screech mode for an axisymmetric jet. The Reynolds-averaged Navier-Stokes equations in the conjunction with a modified Spalart-Allmaras turbulence model are employed. A high resolution finite volume essentially non-oscillatory(ENO) schemes are used along with nonreflecting characteristic boundary conditions that are crucial to screech tone computations to accurately capture the sound waves, shock-cell structures and large-scale instability waves.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2007.04a
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• pp.329-334
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• 2007

An axisymmetric supersonic jet screech in the Mach number range from 1.07 to 1.2 is numerically simulated. The axisymmetric mode is the dominant screech mode for an axisymmetric jet. The Reynolds-averaged Navier-Stokes equations in the conjunction with modified Spalart-Allmaras turbulence model are employed. A high resolution finite volume essentially non-oscillatory(ENO) schemes are used along with nonreflecting characteristic boundary conditions that are crucial to screech tone computations to accurately capture the sound waves, shock-cell structures, unsteady shock motions and large-scale instability waves.