• Asian Pacific Journal of Cancer Prevention
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• 제16권7호
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• pp.2777-2783
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• 2015

Background: Nestin is associated with neoplastic transformation. However, the mechanisms by which nestin contributes regarding invasion and malignancy of gastric adenocarcinoma (GAC) remain unknown. Recent studies have shown that the epithelial-mesenchymal transition (EMT) is important in invasion and migration of cancer cells. In the present study, we aimed to investigate the expression of nestin and its correlation with EMT-related proteins in GAC. Materials and Methods: The expression of nestin and EMT-related proteins was examined in GAC specimens and cell lines by immunohistochemistry and Western blotting. Clinicopathological features and survival outcomes were retrospectively analyzed. Results: Positive nestin immunostaining was most obviously detected in the cytoplasm, nucleus or both cytoplasm and nucleus of tumor cells in 19.2% (24/125) of GAC tissues, which was significantly higher than that in normal gastric mucosa tissues (1.7%, 1/60) (p=0.001). Nestin expression was closely related to several clinicopathological factors and EMT-related proteins (E-cadherin, vimentin and Snail) and displayed a poor prognosis. Interestingly, simultaneous cytoplasmic and nuclear nestin expression correlated with EMT-related proteins (E-cadherin, vimentin and Snail) (p<0.05) and lymph node metastasis (p=0.041) and a shorter survival time (p<0.05), but this was not the case with cytoplasmic or nuclear nestin expression. Conclusions: Nestin, particularly expression in both cytoplasm and nucleus, might be involved in regulating EMT and malignant progression in GAC, with potential as an unfavorable indicator in tumor diagnosis and a target for clinical therapy.

• 한국응급구조학회지
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• 제15권2호
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• pp.43-54
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• 2011

Purpose: The purpose of this study was to explore EMT-paramedic students' experience of simulation education and analyze the confidence before and after education, learning attitude and course evaluation. Method: Research survey was conducted on 38 EMT-paramedic students during November, 2011 and EMT-paramedic students' experience of simulation education was analyzed after applying head, spinal, and chest injury scenario. The confidence before and after education, learning attitude and course evaluation in gender were analyzed by Mann-Whitny U test and the difference of confidence before and after education was analyzed by Wilcoxon signed rank test and learning attitude & course evaluation were analyzed by evaluating frequency, percentage, mean, standard deviation by using SPSS WIN 17.0 program. Results: 1. Students experienced various advantages such as increasing interest and self-reflection on learning, critical thinking ability, and EMT-paramedic-role experience and recognition of importance of teamwork. Students also pointed out disadvantages such as gap between real situation and simulation, limit of time and equipments, and burden of demonstration. 2. The confidence between before and after education, learning attitude and course evaluation in gender were not significant different statistically. 3. Confidence mean score elevated from 5.53(before education) to 5.87(after education), but the difference in their confidence did not show significant difference statistically. 4. Total mean score in learning attitude after simulation education was 3.70 out of 5.00, which is considerably very high. 5. Total mean score in course evaluation was 3.89 with score of 3.83 in evaluation in learning environment and 3.99 in evaluation of debriefing. Conclusion: The finding of this study demonstrate that the simulation education can provide a safe and repetitive practice environment, improve problem-solving ability and critical thinking, and increase the confidence in prehospital emergency care; therefore, simulation may be the new effective EMT-paramedic education strategy.

• 한국응급구조학회지
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• 제10권3호
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• pp.21-32
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• 2006

Purpose: This study was performed to examine the ethical values of EMT students. Method: The results below were based on the revised questionnaires, which can be applicable to EMT from the using questionnaires by Kim(2001), with a sample of 140 students consisting of 50 freshman, 40 sophomore and junior 50, conducted October 9-11, 2006. Data were analyzed using SPSS 13.0 in terms of means and standard deviation, $x^2$-test, one way ANOVA, multiple regression analysis. Results: The ethical value was deontological ethics and statistically significant difference between three groups(F=3.072, p<0.049). Means and standard deviation of freshman is $2.86{\pm}0.28$, sophomore is $2.75{\pm}0.25$, junior is $2.88{\pm}0.21$. In the area of Human life, subjects showed utilitarian disposition. But there were no statistically significant difference between three groups. In the area of patient relationship, all students of three groups took on deontological ethics, but there were no statistically significant difference. In the area of task relationship, all students of three groups took on deontological ethics, but there were no statistically significant difference. In the area of coworker relationship, all students of three groups took on deontological ethics, but there were no statistically significant difference. As a results of multiple regresstion analysis, the associated factors with ethical values were second students, participation of ethical education, a firm belief of ethical values. Conclusion: This results showed that education of ethical values must instruct to EMT students. More and more, It is necessary to make an further application tool of EMT.

• Molecules and Cells
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• 제41권3호
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• pp.188-197
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• 2018

Benzidine, a known carcinogen, is closely associated with the development of bladder cancer (BC). Epithelial-mesenchymal transition (EMT) is a critical pathophysiological process in BC progression. The underlying molecular mechanisms of mitogen-activated protein kinase (MAPK) pathway, especially extracellular regulated protein kinases 5 (ERK5), in regulating benzidine-induced EMT remains unclarified. Hence, two human bladder cell lines, T24 and EJ, were utilized in our study. Briefly, cell migration was assessed by wound healing assay, and cell invasion was determined by Transwell assay. Quantitative PCR and western blot were utilized to determine both gene expressions as well as protein levels of EMT and MAPK, respectively. Small interfering RNA (siRNA) was transfected to further determine ERK5 function. As a result, the migration and invasion abilities were enhanced, epithelial marker expression was decreased while mesenchymal marker expression was increased in human BC cell lines. Meanwhile, benzidine administration led to activation of ERK5 and activator protein 1 (AP-1) proteins, without effective stimulation of the Jun N-terminal kinase (JNK) or p38 pathways. Moreover, Benzidine-induced EMT and ERK5 activation were completely suppressed by XMD8-92 and siRNAs specific to ERK5. Of note, ERK1/2 was activated in benzidine-treated T24 cells, while benzidine-induced EMT could not be reversed by U0126, an ERK1/2 inhibitor, as indicated by further study. Collectively, our findings revealed that ERK5-mediated EMT was critically involved in benzidine-correlated BC progression, indicating the therapeutic significance of ERK5 in benzidine-related BC.

• 한국응급구조학회지
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• 제9권2호
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• pp.183-206
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• 2005

Nowadays, the whole world is putting in the knowledge information society by the internet which is connecting the global network. The internet enable us to see the life of the other people in real time. Most of health care professional obtain and update their new knowledge and skills through continuing education. EMTs who works five or ten shifts per day are not easy to attend continuing education session usually offered during their off-times. One of solutions to this problem would be to develop a education program which does not limit time and place. There has been increasing interest in the use of computers in medical education, but all designated facilities and teachers have no sufficient preparations for the education and training of EMT. There has been less attention to the rationale for the use and selection of applications. This article discusses an approach to the selection of medical informatics applications for the undergraduate medical curriculum. The aim of this study is to develop and evaluate a web-based continuing education program, to help EMT improve their knowledge of transfusion therapy and medical law, and to enhance their ability to contribute to the quality of patient care. The program generally was well received by EMTs. The program is a useful adjunct to the existing continuing education program. It also could be used in EMT student education and other health sciences.

• 한국산학기술학회논문지
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• 제14권6호
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• pp.2908-2914
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• 2013

본 연구는 대전,충남 소재 대학병원에 근무하는 응급의료종사자 122명을 대상으로 병원 내 응급구조사를 조사하여 응급구조사의 역할과 법적 업무규정의 현실적 제고 및 병원 내 응급구조사의 업무에 관한 인식을 보다 명확히 하기 위한 기초자료를 제공 하고자 연구를 실시하였다. 연구 대상자들의 병원 내 응급구조사의 업무인식을 살펴본 결과, 전체 40문항에 대해 $3.23{\pm}0.67$점으로 나타나 병원 내 응급구조사에 대한 업무인식정도는 중간 보다 다소 높은 것으로 나타났다. 영역별로는 외상처치가 가장 높게 나타났으며, 약물투여가 가장 낮았다. 응급의료종사자가 응급구조사의 업무를 바르게 인식하게 하기 위해서는 응급구조사가 고유 업무와 역할을 충실히 해 낼 수 있어야 할 것이다. 그러므로 보다 명확한 교육과정이 이루어져야 하며 보다 명확한 업무가 법적으로 규정되어져야 한다. 응급구조사에 대한 홍보와 지속적인 모니터링이 필요할 것으로 사료된다.

• Biomolecules & Therapeutics
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• 제25권6호
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• pp.625-633
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• 2017

Sphingosylphosphorylcholine (SPC) is one of the bioactive phospholipids that has many cellular functions such as cell migration, adhesion, proliferation, angiogenesis, and $Ca^{2+}$ signaling. Recent studies have reported that SPC induces invasion of breast cancer cells via matrix metalloproteinase-3 (MMP-3) secretion leading to WNT activation. Thrombospondin-1 (TSP-1) is a matricellular and calcium-binding protein that binds to a wide variety of integrin and non-integrin cell surface receptors. It regulates cell proliferation, migration, and apoptosis in inflammation, angiogenesis and neoplasia. TSP-1 promotes aggressive phenotype via epithelial mesenchymal transition (EMT). The relationship between SPC and TSP-1 is unclear. We found SPC induced EMT leading to mesenchymal morphology, decrease of E-cadherin expression and increases of N-cadherin and vimentin. SPC induced secretion of thrombospondin-1 (TSP-1) during SPC-induced EMT of various breast cancer cells. Gene silencing of TSP-1 suppressed SPC-induced EMT as well as migration and invasion of MCF10A cells. An extracellular signal-regulated kinase inhibitor, PD98059, significantly suppressed the secretion of TSP-1, expressions of N-cadherin and vimentin, and decrease of E-cadherin in MCF10A cells. ERK2 siRNA suppressed TSP-1 secretion and EMT. From online PROGgene V2, relapse free survival is low in patients having high TSP-1 expressed breast cancer. Taken together, we found that SPC induced EMT and TSP-1 secretion via ERK2 signaling pathway. These results suggests that SPC-induced TSP-1 might be a new target for suppression of metastasis of breast cancer cells.

• Molecules and Cells
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• 제37권4호
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• pp.330-336
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• 2014

Thymosin beta4 (TB4) has multiple functions in cellular response in processes as diverse as embryonic organ development and the pathogeneses of disease, especially those associated with cardiac coronary vessels. However, the specific roles played by TB4 during heart valve development in vertebrates are largely unknown. Here, we identified a novel function of TB4 in endothelial-mesenchymal transformation (EMT) in cardiac valve endocardial cushions in zebrafish. The expressions of thymosin family members in developing zebrafish embryos were determined by whole mount in situ hybridization. Of the thymosin family members only zTB4 was expressed in the developing heart region. Cardiac valve development at 48 h post fertilization was defected in zebrafish TB4 (zTB4) morpholino-injected embryos (morphants). In zTB4 morphants, abnormal linear heart tube development was observed. The expressions of bone morphogenetic protein (BMP) 4, notch1b, and hyaluronic acid synthase (HAS) 2 genes were also markedly reduced in atrio-ventricular canal (AVC). Endocardial cells in the AVC region were stained with anti-Zn5 antibody reactive against Dm-grasp (an EMT marker) to observe EMT in developing cardiac valves in zTB4 morphants. EMT marker expression in valve endothelial cells was confirmed after transfection with TB4 siRNA in the presence of transforming growth factor ${\beta}$ ($TGF{\beta}$) by RT-PCR and immunofluorescent assay. Zn5-positive endocardial AVC cells were not observed in zTB4 morphants, and knockdown of TB4 suppressed TGF-${\beta}$-induced EMT in ovine valve endothelial cells. Taken together, our results demonstrate that TB4 plays a pivotal role in cardiac valve formation by increasing EMT.

• 융합정보논문지
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• 제11권11호
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• pp.248-255
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• 2021

본 연구는 만성폐쇄성폐질환(COPD)의 동물 모델을 이용하여 Celecoxib의 폐 손상 개선효과를 연구하였다. COPD는 LPS와 담배연기추출물(CSE)로 유도하여 in vitro와 in vivo에서 병행 연구하였다. In vitro는 인간 섬유아세포(MRC5)에서 MTT assay, real-time PCR를 하였고 in vivo는 mRNA 발현, 기관지폐포세척액(BALF), collagen content, 단백질 발현을 확인하였다. 실험을 통해 Celecoxib는 BALF에서 염증세포 수의 감소와 사이토카인, soluble protein의 축적을 감소시켰고 동물모델에서는 체중과 폐 무게를 감소시켰으며, 폐 콜라젠 축적도 개선하였다. 또 웨스턴 블로팅과 real-time PCR을 통해 EMT 표지자의 감소를 확인하였다. 결과적으로 Celecoxib는 EMT를 조절하여 LPS+CSE로 유도된 COPD의 폐 손상에서 개선제로 작용할 수 있을 것으로 사료된다.

• BMB Reports
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• 제56권2호
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• pp.120-125
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• 2023

Karyopherin-α3 (KPNA3), a karyopherin-α isoform, is intimately associated with metastatic progression via epithelial-mesenchymal transition (EMT). However, the molecular mechanism underlying how KPNA3 acts as an EMT inducer remains to be elucidated. In this report, we identified that KPNA3 was significantly upregulated in cancer cells, particularly in triple-negative breast cancer, and its knockdown resulted in the suppression of cell proliferation and metastasis. The comprehensive transcriptome analysis from KPNA3 knockdown cells indicated that KPNA3 is involved in the regulation of numerous EMT-related genes, including the downregulation of GATA3 and E-cadherin and the up-regulation of HAS2. Moreover, it was found that KPNA3 EMT-mediated metastasis can be achieved by TGF-β or AKT signaling pathways; this suggests that the novel independent signaling pathways KPNA3-TGF-β-GATA3-HAS2/E-cadherin and KPNA3-AKT-HAS2/E-cadherin are involved in the EMT-mediated progress of TNBC MDA-MB-231 cells. These findings provide new insights into the divergent EMT inducibility of KPNA3 according to cell and cancer type.