• Title/Summary/Keyword: EHV-1

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Protective Immune Reponses Induced by Non-infectious L-particles of Equine Herpesvirus Type-1: Implication of Cellular Immunity

  • Mohd Lila Mohd Azmi;Field, Hugh-John;Frazer Rixon;Lauchlan, John-Mc
    • Journal of Microbiology
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    • v.40 no.1
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    • pp.11-19
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    • 2002
  • Mice immunized with equine herpesvirus type-1(EHV-1) L-particles skewed a significant increase (p<7.75) in serum antibody titers. Upon a booster dose four weeks lateral antibody titers increased significantly. Interestingly, immunization via intravenous or intramuscular route induced significantly higher (p<0.75) antibody titers. However, mice iummunized with UV-treated L-particles, visions or immunization via intranasal route induced lower antibody titers. Upon challenge inoculation with wildtype EHV-1, our data showed there was a poor correlation between antibody titers and protection against virus replication. Therefore, the role of cell-mediated immunity Inwards protection was investigated. As predicted, the strongest cell-mediated immunity, as measured by delayed-hypersensitivity test, was detected in mice immunized with live virus particles. The magnitude of cell-mediated immune response correlated with the efficacy of L-particles as immunizing agent. The highest efficacy, as indicated in mice immunized via intranasal routed was highly correlated with cell-mediated immunity. A similar phenomenon was also demonstrated in mice immunized intranasally with UV-treated L-particles. However, the degree of protection was reduced when mice immunized intravenously or intramuscularly with UV-treated L-particles. In conclusion, protection conferred in these animals was highly implicated by immune cells and the least by antibodies. The route of immunization and the nature of the antigen also contributed to the efficacy of L-particles as immunizing agent. In contrast to that of herpes simplex virus type 1, our data showed EHV-1 non-infectious L-particles are highly suitable for immunization of the host against EHV-1 disease.

Detection of equine herpesvirus type-1 in naturally aborted equine fetuses in Jeiu by polymerase chain reaction (제주도내 자연발생한 말유산태아에서 PCR을 이용한 Equine Herpesvirus Tyre-1 검출)

  • Moon, Hyuk;Kang, Wan-Cheul;Kim, Eun-Joo;Kim, Jin-Hoe;Ko, Hyun-Joo;Yang, Jae-Hyuk;Son, Won-Geun;Lee, Du-Sik
    • Korean Journal of Veterinary Service
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    • v.24 no.1
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    • pp.83-88
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    • 2001
  • It is impotent to identify the causative agents of abortion in equine for minimizing the loss of breeding costs in equine Industry. Recently, the abortion has often occurred in equine herds and thus the purpose of the study was aimed at the identification of equine herpesvirus-1, one of the frequent pathogens to abortion, using polymerase chain reaction. Six fetuses to be aborted at nine to ten months in pregnancy reared in six herds were used in the study. Two primers in the PCR were made from glycoprotein B gene of EHV-1. The primers specific for EHV-1 amplified 1880 bp of PCR products from DNA extracts from thorax fluids, livers, lungs, and spleens of four in six aborted fetuses. Consequently, PCR could be applied to diagnose the abortion of EHV-1 and also confirmed to play a major role of the viral pathogen associated with equine abortion in Jeju island.

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Studies on isolation of rhinopneumonitis virus from Korean horses and its immunogenicity I. Sero-epidemiological studies on equine herpesvirus-1 (국내 말로부터 비폐렴바이러스의 분리 및 면역원성에 관한 연구 I. 말 비폐렴바이러스(equine rhinopneumonitis virus)에 대한 혈청학적 역학조사)

  • Cho, Gil-jae;Kim, Bong-hwan;Lee, Du-sik;Shin, Tae-kyun;Yang, Ki-chun;Lim, Yoon-kyu;Cho, Sung-soo
    • Korean Journal of Veterinary Research
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    • v.35 no.4
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    • pp.735-741
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    • 1995
  • The present study was conducted to investigate the incidence of equine herpesvirus 1(EHV-1) infection in Korean horses by cell EUSA and serum neutralization test A significant level of antibodies to EHV-1 was detected from 539(82.8%) by cell EUSA and from 524 horses(80.5%) by SNT indicating that EHV-1 infection in Korean horses are widely spreaded throughout the country.

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국내소식 학계시보

  • 대한전기학회
    • 전기의세계
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    • v.14 no.2
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    • pp.53-55
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    • 1965
  • 본고의 내용은 다음과 같다. 1. 위상측정에 민감한 교직변환기 2. EHV 개폐시험 및 개폐써어지 3. 선형화에 의한 비선형 귀환계의 설계 4. 초도전재료의 응용에 관한 검토

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Development of Evaluating Technology for the Capability of Carrying Short-Circuit Current at Electrical Contacts in EHV Disconnecting Switches (초고압 단로기 접점의 단락전류 통전성능 평가기술 개발)

  • Oh, Yeon-Ho;Song, Ki-Dong;Chong, Jin-Kyo
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.57 no.1
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    • pp.46-51
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    • 2008
  • Extra-high voltage(EHV) disconnecting switch(DS) consists of the electrical contacts and mechanical parts which actuate the contacts. When the short-circuit condition occurs, a large amount of current flows through the electrical contact in disconnecting switches and this causes considerable temperature rise due to Joule heating. If the temperature rise is higher than the melting point of contact material, the DS contact becomes melting and cannot be usable anymore. For this reason, the analysis for capability of carrying short-circuit current in DS contacts must be performed at a design stage. Here, we proposed a numerical technique for evaluating the capability of carrying short-circuit current at electrical contacts in EHV DS. In this numerical approach, the mechanical and thermal analyses were simulated to check the capability of carrying short-circuit current. First, the applied pressure at contact parts was analyzed considering the mechanical properties, and then contact resistance was calculated by an empirical equation. Finally, thermal analysis was performed with resistance variation at electrical contacts. To verify these numerical results, the distributions of temperature in DS were experimentally measured and compared with each other. The results from experiments were agreed well with those from the proposed numerical simulations.

Protection of Specific-pathogen-free (Spf) Foals from Severe Equine Herpesvirus Type-1 (Ehv-1) Infection Following Immunization with Non-infectious L-particles

  • Mohd Lila Mohd-Azmi;John Gibson;Frazer Rixon;Lauchlan, John-Mc;Field, Hugh-John
    • Journal of Microbiology
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    • v.40 no.3
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    • pp.183-192
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    • 2002
  • Cells infected With equine herpesvirus type-1 (EHV-1) Produced both infectious and non-infectious Virus-related particles. Compared to the whole virion, non-infectious particles termed L-particles were deter-mined to lack 150 kDa protein, commonly known as nucleocapsid protein. The potential of L-particles to induce immune responses was studied in mice and foals. Intranasal immunization with L-particles or whole virions induced poor IgG antibody responses in mice. Interestingly, despite the poor antibody response, the conferred immunity protected the host from challenge infections. This was indicated by a significant reduction in virus titers in line with recovery towards normal body weight. Subsequently, the test on the usefulness of L-particles as immunizing agents was extended to foals. Immunization of specific-pathogen-free (SPF) foals resulted in similar results. As determined by a complement-fixing-antibody test (CFT), foals seroconverted when they were immunized either with inactivated L-particles or whole virions via intramuscular (i.m.) injections. The presence of the antibody correlated with the degree of protection. Beyond day 1 post challenge infection (p.i.), there was no virus shedding in the nasal mucus of foals immunized with whole EHV-1 virions. Virus shedding was observed in foals Immunized with L-particles but limited to days 6 to 8 p.i. only. In contrast, extended vim shedding was observed in non-immunized foals and it was well beyond day 14 p.i. Viremia was not detected for more than four days except in non-immunized foals. Immunization in mice via intranasal (i.n.) conferred good protection. However, compared to the i.n. route, a greater degree of protection was obtained in foals following immunization via i.m. route. Despite variation in the degree of protection due to different routes of immunization in the two animal species, our results have established significant evidence that immunization with L-particles confers protection in the natural host. It is suggested that non-infectious L-particles should be used as immunizing agents for vaccination of horses against EHV-1 infection.

Analysis of Transient Characteristics of Air-Gapped CTs In EHV Transmission Systems (초고압 계통에서의 공극 변류기의 과도 특성 분석)

  • Kang, Y.C.;Yun, J.S.;Lee, B.E.;Kang, S.H.;Park, J.K.
    • Proceedings of the KIEE Conference
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    • 1999.11b
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    • pp.294-296
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    • 1999
  • This paper describes the transient characteristics of air-gapped CTs in EHV transmission systems. An air-gapped CT is modelled using EMTP. Air-gapped CTs with the gap of 0, 0.5, 1.0 [mm] are analysed and compared with and without consideration of auto-reclosure. From the results. even small gap improves the transient characteristics after auto-reclosure.

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Electric Field Distribution and AC Dielectric Breakdown Properties according to Needle Electrode in EHV Insulators of XLPE (초고압 절연체 XLPE의 침전극에 따른 전계분포와 AC 절연파괴특성)

  • An, Byung-Chul;Hong, Jin-Woong
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2006.05a
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    • pp.136-139
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    • 2006
  • 최근 전력수요의 급증과 더불어 기기의 소형화와 경랑화를 위해 고분자절연재료의 사용이 증가하였으며, 근래 들어 환경성의 문제가 거론되면서 반영구적인 절연재료의 요구가 급증하였다. 그로 인하여 초고압 케이블의 절연재료로서 가교폴리에틸렌이 사용되어지고 있다. 이에 본 논문에서는 초고압전력용 케이블에서 절연재료로 사용되고 있는 가교폴리에틸렌 (XLPE) 내부전계분포 및 파괴전압과의 상관관계를 알아보기 위해 케이블에 칩 전극을 삽입하여 두께를 0.5, 1, l.5 [mm] 변화시켜 파괴전계를 검출하였으며, 또한 와이블 해석을 통한 파괴전압의 척도파라미터룰 검출하여, 시뮬레이션 인가전압으로 사용하였다. 시뮬레이션 경계요소법 (BEM)을 이용한 3 차원 전계해석 프로그램으로 조사하였다.

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Studies on isolation of rhinopneumonitis virus from Korean horses and its immunogenicity III. Studies on immunogenicity of equine herpesvirus in experimental animals (국내 말로부터 비폐렴바이러스의 분리 및 면역원성에 관한 연구 III. 말 비폐렴바이러스의 실험동물에 대한 면역원성 조사)

  • Cho, Gil-jae;Kim, Bong-hwan;So, Sin-hee;Lee, Du-sik
    • Korean Journal of Veterinary Research
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    • v.35 no.4
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    • pp.753-758
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    • 1995
  • The study was carried out to investigate the pathogenicity of EHV isolate to hamsters and mice and immunogenicity of experimentally produced. vaccine were evaluated in the horses. Hamsters infected. intranasally with $LC_1$ isolate showed symptoms of nasal discharge, conjunctivitis and body weight loss during the observation period of 12 days after infection, while only slight depression and body weight loss were noticed with mice infected with $LC_1$ indicating that hamsters are more susceptible to the virus. Antibody titer of mice and hamsters were gradually increased to highest level of 1:2560~10240, 1:640~1280, respectively, at 7~12 days post vaccination. Horses immunized against $LC_1$ killed vaccine reached to maximum antibody titer of 1:20480 around 4 weeks after 1st vaccination and declined after 12 weeks post vaccination. No significant antibody increase were detected after 2nd vaccination. Mean body temperature and mean total leukocyte counts remained within normal range and no adverse reaction were noticed after vaccination.

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Assessment and Management Method of Flicker Emission Level Based on IEC 61000-3-7 for Domestic Extra-high Voltage Customers (국내 특고압 고객에 대한 IEC 61000-3-7 기반의 플리커 방출한계 평가 및 관리 방안 연구)

  • Han, Su-Kyoung;Shin, Hoon-Chul;Park, Sang-Ho;Kim, Kern-Joong;Cho, Soo-Hwan
    • The Transactions of The Korean Institute of Electrical Engineers
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    • v.67 no.1
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    • pp.1-7
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    • 2018
  • IEC 61000-3-7 provides guidance for limiting flicker and enabling the connection of fluctuating load installations, that is, producing flicker in MV, HV and EHV power systems. In Korea, the flicker have been restricted by Japanese standard of ${\Delta}V_{10}$ method. ${\Delta}V_{10}$ was developed only for arc furnaces in 1960's. And now it is revealed that it is not suitable for application to other fluctuating load installations through many researches. $P_{st}$ which is a flicker index used in IEC 61000-3-7, indicates visual inconvenience due to voltage fluctuation across large range of frequency and can be applied to fluctuating load installations as well as arc furnaces. In this paper, we introduce how to calculate and assess flicker emission level for the individual fluctuating load installations connected in EHV system and how to manage the emission levels in the power system according to IEC 61000-3-7.