Korean Journal of Veterinary Service (한국동물위생학회지)
- Volume 24 Issue 1
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- Pages.83-88
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- 2001
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- 3022-7372(pISSN)
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- 2287-7630(eISSN)
Detection of equine herpesvirus type-1 in naturally aborted equine fetuses in Jeiu by polymerase chain reaction
제주도내 자연발생한 말유산태아에서 PCR을 이용한 Equine Herpesvirus Tyre-1 검출
- Moon, Hyuk (Jeju Livestock Promotion Institute) ;
- Kang, Wan-Cheul (Jeju Livestock Promotion Institute) ;
- Kim, Eun-Joo (Jeju Livestock Promotion Institute) ;
- Kim, Jin-Hoe (Jeju Livestock Promotion Institute) ;
- Ko, Hyun-Joo (Department of Veterinary Medicine, Jeju National University) ;
- Yang, Jae-Hyuk (Korea Racing Association) ;
-
Son, Won-Geun
(Department of Veterinary Medicine, Jeju National University)
;
-
Lee, Du-Sik
(Department of Veterinary Medicine, Jeju National University)
- 문혁 (제주도축산진흥원) ;
- 강완철 (제주도축산진흥원) ;
- 김은주 (제주도축산진흥원) ;
- 김진희 (제주도축산진흥원) ;
- 고현주 (제주대학교 수의학과) ;
- 양재혁 (한국마사회) ;
-
손원근
(제주대학교 수의학과)
;
-
이두식
(제주대학교 수의학과)
- Published : 2001.04.01
Abstract
It is impotent to identify the causative agents of abortion in equine for minimizing the loss of breeding costs in equine Industry. Recently, the abortion has often occurred in equine herds and thus the purpose of the study was aimed at the identification of equine herpesvirus-1, one of the frequent pathogens to abortion, using polymerase chain reaction. Six fetuses to be aborted at nine to ten months in pregnancy reared in six herds were used in the study. Two primers in the PCR were made from glycoprotein B gene of EHV-1. The primers specific for EHV-1 amplified 1880 bp of PCR products from DNA extracts from thorax fluids, livers, lungs, and spleens of four in six aborted fetuses. Consequently, PCR could be applied to diagnose the abortion of EHV-1 and also confirmed to play a major role of the viral pathogen associated with equine abortion in Jeju island.