• Proceedings of the Korean Information Science Society Conference
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• 2004.04b
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• pp.367-369
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• 2004

기존의 연구비 관리업무는 예산계획, 예산편성 및 예산정산 부분으로 관리됨으로써 연구비의 안정적인 확보와 효율적인 운영 및 투명한 집행을 위하여 통합관리가 절실하게 요구되고 있는 실정이다. 에러한 추세를 반영하고자 기존의 연구비 관리 프로그램을 재사용하기 위해 모바일 협업 비즈니스 모듈을 개발하여 모바일 기기의 특성에 적용할 수 있도록 특화된 협업 프로세스를 지원하는 새로운 형태의 모바일 협업 컴포넌트를 개발하였다. 이러한 결과 사용자의 요구사항을 최대한 반영하여 연구비 관리정책의 수립에 필요한 다양한 형태의 의사결정 지원정보를 제공할 수 있도록 하였다. 또한, 서로 다른 목적으로 운영되던 시스템을 통합하여 시스템의 총 운영비용을 줄이고 실시간으로 의사결정에 참여할 수 있도록 하였다.

• Proceedings of the Korean Ceranic Society Conference
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• 2003.04a
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• pp.59.2-59
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• 2003

• Proceedings of the Korean Ceranic Society Conference
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• 2003.10a
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• pp.222.2-222
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• 2003

• Reproductive and Developmental Biology
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• v.35 no.1
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• pp.55-60
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• 2011

The objective of this study was carried out to evaluate the efficiency of sperm collection methods on the post-thaw viability of cat semen. The cat semen was collected by artificial virginal (AV) and electronic ejaculate (EE) methods. The composition of semen extender was consisted of Tris-buffer supplemented with 20% egg yolk and 1% P/S antibiotics in Ext I, and more added 8% glycerol, 1.0% Equex STM paste of total volume in Ext II. The collected semen was adjusted the concentration and then diluted in Ext I for optimal concentration. The diluted semen was cooling to $5^{\circ}C$ temperature in refrigerator for at least 2 hrs and then diluted stepwise with Ext II for at least 1 hrs. After an equilibration for 1 hrs, the cooled semen was packaged in 0.5 ml straw and then freezing on the $LN_2$ vapor over 5 cm above from $LN_2$ and then immersed directly in $LN_2$ for cryopreservation. The frozen semen was thawed in $38^{\circ}C$ water for 15 sec and then evaluated the motility, viability, and morphology. Post-thaw semen were calculated the motility by SMI (sperm motility index). The live-dead sperm was evaluated by Eosin-B and morphological evaluation was by Diff-quik kit staining. The post-thaw concentration ($89{\times}10^6$ /ml vs. $128{\times}10^6$ /ml), viability ($22.6{\pm}10.6%$ vs. $37.1{\pm}26.1%$), morphological normality ($27.0{\pm}50.2%$ vs. $45.6{\pm}123.0%$) of EE and AV groups were not significant different, but the post-thaw motility was significant lower in EE than that in AV group ($53.1{\pm}3.6$ vs. $73.6{\pm}5.7$) (p<0.05). In conclusion, semen collection methods did not significant different between EE and AV groups except of post-thaw motility and so both semen collection methods could be applied in feline semen collection methods.

• Asian-Australasian Journal of Animal Sciences
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• v.18 no.2
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• pp.158-164
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• 2005

A practical approach was proposed to produce transgenic chimeric chickens using blastodermal cells (BCs). The chicken BCs were mechanically dissociated and transferred into the recipient eggs that had been exposed to 500 rads irradiation of$^{60}Co$ and windowed on the equatorial plane. Chimeric chickens were generated using two models: the crosses (MXL) from Black Minors (ii,EE,b/b) ♂${\times}$Barred Leghorns (ii,ee,B/-) ♀ as donors and White Leghorns (WL, II) as acceptors (Model 1), or the Black Heifengs (BH, ii,EE,bb) as donors and Hua-xing white (HW, II) as recipients (Model 2). The treated eggs were incubated in their original shells in normal conditions until hatching. Green fluorescent protein (GFP) gene was transferred into the BCs derived from MXL and BH via lipofectamine and the pEGFP-C1, and transfection efficiency into the BCs was examined under a fluorescent microscope. Potential transgenic chimeras were selected based on the proposed methods in this study. Using the fresh BCs, the best rate of phenotypic chimeras was 6.7% and 26.0% in model-1 groups, and model-2 groups, respectively. We also described the optimized conditions for transfection. Although 30% of the BCs transfected in vitro emitted green light under an inverted fluorescent microscope, no embryos injected with the transfected BCs expressed foreign GFP gene at 3-4 days.

• KIEE International Transactions on Electrophysics and Applications
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• v.12C no.4
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• pp.201-207
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• 2002

A Pin-Cylinder discharge type ozonizer was designed and manufactured. The increase or decrease of temperature greatly influences on the characteristics of ozone generation of a discharge type ozonizer. The characteristics of ozone concentration ( $O_{3con}$), ozone generation ($_{3g}$) and ozone yield .ate ($_{3Y}$) of the ozonizer were investigated by varying the gas flow rate (Q), the discharge power ($_{Wd}$ ) and the temperature (T). At T = 20($^{\circ}C$), the values of ( $O_{3con}$) were found as 7800,5300,3000 and 2300(ppm) at Q=1,2,4 and 6(1/min) respectively. The corresponding values of ( $O_{3g}$) were found as 917, l247,1411 and 1623(mg/h) and those of ( $O_{3Y}$) were 93,126,143 and 164(g/kWh) respectively. When the temperature is decreased to -50($^{\circ}C$), the values of ( $O_{3con}$) became 12000,8000,5200 and 3600(ppm) at Q=1,2,4 and 6(1/min) respectively. The corresponding values of 0,9 were obtained as 1411,1882,2446 and 2600(mg/h) and those of ($O_{3Y}$) were 143,190,247 and 2631g/kWh] respectively. Hence as the temperature was decreased from 20 to -50[。C], the efficiencies of ozone generation were increased by 54,51,73 and 60[%] at Q=1,2,4 and 6(1/min) respectively.ctively.

• Journal of Korean Biological Nursing Science
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• v.10 no.2
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• pp.176-183
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• 2008

Purpose: The purpose of this study was to identify the effect of Environmental Enrichment (EE) on improvement of motor function in animal models of Parkinson's Disease. Methods: Male C57BL6 mice weighing 25-30 g, at the age of 12 wks were used in this study. The animals were injected MPTP (1-methyl-4-phenyl-1,2,3,6-tetrahydropyridin, 20 mg/kg in saline, i.p.) 4 times a day at every 2 hr, and raised in EE cage for 14 days. On day 14, after behavior test, all mice were sacrificed for immunohistochemistry. All values were expressed as means$\pm$S.E.M. Statistical significance was evaluated using a one way ANOVA followed by Sheffe test. Results: There was a significant difference between the experimental group and the control group in the behavior test. Also EE significantly reduced of TH positive cell loss in Substantia nigra pars compacta as compared to the result of MPTP treatment. Conclusion: Based on these findings, it is reasonable to assume that the environmental enrichment prevents dopaminergic neuronal loss and improves disarrangement of motor function and behavioral disability induced by MPTP.

• Journal of Life Science
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• v.13 no.6
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• pp.794-798
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• 2003

Batch production of (S)-phenyl oxirane was investigated using epoxide hydrolase activity of Rhodosporidium toruloides SJ-4. Effect of reaction condition of asymmetric biohydrolysis of racemic phenyl oxirane was analyzed and optimized by response surface methodology. The optimal conditions of pH, temperature and DMSO cosolvent ratio were 7.4, $34^P\circ}C$, and 2.3%(v/v), respectively. The final yield was enhanced up to 67%, and reaction times required to reach 99% ee (enatiomeric excess) decreased down to 50% by response surface methodology Enantiopure (S)-phenyl oxirane with 100% enantiopurity and 24% yield (theoretical yield = 50%) was obtained from racemic substrate.

• Journal of IKEEE
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• v.7 no.2 s.13
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• pp.236-244
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• 2003

In this paper, RF and IF circuits for mobile terminals which have usually been implemented using expensive BiCMOS processes are designed using CMOS circuits, and a Tx CMOS RF/IF single chip for PCS applications is designed. The designed circuit consists of an IF block including an IF PLL frequency synthesizer, an IF mixer, and a VGA and an RF block including a SSB RF mixer and a driver amplifier, and performs all transmit signal processing functions required between digital baseband and the power amplifier. The phase noise level of the designed IF PLL frequency synthesizer is -114dBc/Hz@100kHz and the lock time is less than $300{\mu}s$. It consumes 5.3mA from a 3V power supply. The conversion gain and OIP3 of the IF mixer block are 3.6dB and -11.3dBm. It consumes 5.3mA. The 3dB frequencies of the VGA are greater than 250MHz for all gain settings. The designed VGA consumes 10mA. The designed RF block exhibits a gain of 14.93dB and an OIP3 of 6.97dBm. The image and carrier suppressions are 35dBc and 31dBc, respectively. It consumes 63.4mA. The designed circuits are under fabrication using a $0.35{\mu}m$ CMOS process. The designed entire chip consumes 84mA from a 3V supply, and its area is $1.6㎜{\times}3.5㎜$.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.40 no.3
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• pp.602-612
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• 2015

Current IEEE 802.11p which is suggested for vehicle to vehicle communication supports one hop communication. Thus, it has a limitation to carry out efficient data dissemination. In this thesis, we suggest LTE-D2D based vehicle network to provide efficient data dissemination in the vehicle environment. In this network architecture, we use name based message with IP packet options and we put the intermediate vehicle node called 'super vehicle node' and each normal vehicle node in the cell requests data to the super vehicle node. The super vehicle node responses data to the normal vehicle node. Performance analysis is based mathematical modeling. We compare LTE cellular network to LTE-D2D based vehicle network about throughput according to packet delivery time.