• Title/Summary/Keyword: EAG

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Characterizations of Restriction Endonuclease EagBI from Enterobacter agglomerans CBNU45 (Enterobacter agglomerans CBNU45로부터 분리된 제한효소 EagBI 의 특성)

  • Choe, Yeong-Ju;Kim, Seong-Jae;Hwang, Hye-Yeon;Im, Jeong-Bin;Kim, Yeong-Chang
    • Korean Journal of Microbiology
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    • v.32 no.1
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    • pp.91-95
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    • 1994
  • EagBI is a type II restriction endonuclease from Enterobacter agglomerans strain CBNU45 isolated from soil. EagBI was partially purified by DEAE-cellulose, phosphocellulose P11 and hydroxylapatite column chromatography. EagBI recognizes and cleaves the sequence 5'-CGAT${\downarrow}$CG-3' and generates 2-base 3'-protruding cohesive ends. The optimal reaction conditions of EagBI are 10 mM Tris-HCl (pH 7.8), 6-10 mM $MgCl_2$, at 37 ${\circ}C$. The enzyme is maximally active in the absence of NaCl, able to cleave both $dam^-$ and $dam^+$ DNAs, and sensitive to heat treatment (at 65 ${\circ}C$ for 10 min). Therefore, although EagBI is an isoschizomer of PvuI, it is more useful than PvuI in respect of the NaCl requirement and heat-stability.

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Characteristics of Electroantennogram Parameters for the Detection of Odorants

  • Yun, Eung-Sik;Ko, Hwi-Jin;Sun, Jong-Hwan;Park, Tai-Hyun
    • Journal of Microbiology and Biotechnology
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    • v.10 no.6
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    • pp.885-888
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    • 2000
  • Electroantennogram(EAG) can be applied to detect odorants since insects have highly specialized olfactory receptors inside their antennae. The characteristics of EAG parameters were investigated for the quantitative measurement of a general odorant using ammonia as a model odorant. The antennae of male silkworm moth, Bombyx mori, were used for the EAG. The electical signal curves generated from a pair of antennae originating from the same silkworm moth were never identical; however, they exhibited a typical type I or type II characteristic curve pattern for every pair of antennae. The correlation between the EAG parameters and the ammonia concentration was analyzed for the type I and type II antennae. The stability of each parameter was also investigated for each type of antenna. The results show the possibility of the quantitative measurement of general odorants using the EAG technique.

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Apoptosis in Leukemic Cells Induced by Anti-Proliferative Coumarin Isolated from the Stem Bark of Fraxinus rhynchophylla

  • Lee, Beom Zoo;Lee, Ik Soo;Pham, Chau Ha;Jeong, Soon-Kyu;Lee, Sulhae;Hong, KwangWon;Yoo, Hee Min
    • Journal of Microbiology and Biotechnology
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    • v.30 no.8
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    • pp.1214-1221
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    • 2020
  • Esculetin 6-O-β-D-arabinofuranosyl-(1 → 6)-β-D-glucopyranoside (EAG) is a coumarin glycoside isolated from the stem bark of Fraxinus rhynchophylla. This study scrutinized the anti-proliferative activity of EAG on blood cancer-derived Jurkat leukemic cells. Cell viability assays in leukemic cancer cells determined that EAG possesses potent anti-proliferative effects. Moreover, treatment with EAG increased the proportion of apoptotic cells, resulted in cell cycle arrest being induced at the subG0/G1 phase, and reduced the proportion of cells present in the S phase. In addition, mitochondrial membrane potential was reduced by EAG in Jurkat cells. Additionally, EAG triggered apoptosis that was mediated by the downregulation of BCL-XL, p-IκBα, and p-p65 expressions in addition to the upregulation of cleaved Caspase 3 and BAX expressions. These findings revealed that the toxic effect of EAG was mediated by intracellular signal transduction pathways that involved a mechanism in which reactive oxygen species (ROS) were upregulated. Thus, this study concludes that EAG could potentially serve as a therapeutic agent for leukemia.

Synthesis and Electroantennogram (EAG) Responses of the Sex Phermones of the Diamond Back Moth (배추좀나방 성 페로몬의 합성과 EAG 감응반응 연구)

  • Gang, Seok Gu;Seol, Gwang Yeol;Jeon, Jong Gap;Go, Hyeon Gwan;Kim, Jung Jin
    • Journal of the Korean Chemical Society
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    • v.34 no.2
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    • pp.179-183
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    • 1990
  • New synthesis and electroanthennogram (EAG) responses of the pheromones of the diamond back moth Plutella xylostella L. are described. Lithium dianion of propargyl alcohol was alkylated with 1-bromononane to give 2-dodecyn-1-ol, which was converted to 11-dodecyn-1-ol by acetylene-zipper reaction. Dianion of 11-dodecyn-1-ol was alkylated with 1-bromobutane to provide 11-hexadecyn-1-ol, which was in turn reduced over Pd/BaSO4 to afford (Z)-11-hexadecen-1-ol (3). (Z)-11-Hexadecen-1-ol was oxidized and acetylated to (Z)-11-hexadecen-1-al (1) and (Z)-11-hexadecen-1-yl acetate (2), respectively. The electroantennogram (EAG) responses of the diamond back moth to the pheromone compouds thus synthesized were measured.

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Effect of Acorus Gramineus on the Relaxation of Corpus Cavernosum Smooth Muscle (석창포에 의한 발기부전 개선 효과)

  • Li, Xiang;Kim, Ho-Tae;Lee, Jae-Yun;Lee, Yun-Jung;Shin, Hong-Kyun;Kang, Dae-Gill;Lee, Ho-Sub
    • Journal of Physiology & Pathology in Korean Medicine
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    • v.25 no.5
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    • pp.863-869
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    • 2011
  • The aim of the present study is to investigate whether an ethanol extract of Acorus gramineus Soland (EAG) augments penile erection in vitro and in vivo experiment. Preconstructed with phenylephrine (PE) in isolated endothelium-intact rabbit corpus cavernosum, EAG relaxed penile smooth muscle in a dose-dependent manner, which was inhibited by pretreatment with NG-nitro-L-argininemethylester (L-NAME), a nitricoxide synthase inhibitor, and 1H-[1,2,4]-oxadiazole-[4,3-${\alpha}$]-quinoxalin-1-one (ODQ), a soluble guanylylcyclase (sGC) inhibitor, respectively. EAG-induced relaxation was significantly attenuated by pretreatment with tetraethylammonium (TEA), a nonselective $K^+$ channel blocker. EAG increased cGMP levels of the rabbit corpus cavernosum in a concentration-dependent manner without changes in cAMP levels. In addition, EAG caused increase of peak intracavernous pressure (ICP), ICP/MAP ratio and area under the carve (AUC) in SD rats. Taken together, these results suggest that EAG augments penile erection via NO-cGMP system and $K^+$ channels in corpus cavernosum.

Quantitative Measurement of General Odorant Using Electroantennogram of Male Silkworm Moth, Bombyx mori

  • Yun, Enug-Sik;Park, Tai-Hyun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.5 no.2
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    • pp.150-152
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    • 2000
  • The investigation of electroantennogram (EAG) using insect antennad has been primarily focused on the measurement of insect pheromone. Insect has highly specialized olfactroy receptors inside their antennae. In this paper, EAG was applied to detect general oborants and the feasibility of this system for the olfactroy biosensor was investigated. Electroantennogram measurement was carried out using the antennae of male silkworm moth, bombyx mori, and ammonia gas as the model odorant. EAG parametres including peak ampiltude increased linearly with the ammonia concentration and the reproducible electrical signals were generated at least for 2 hrs after the antenna was cut off from the sulkworm moth.

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Electroantennogram Responses of Spodoptera frugiperda Males (Lepidoptera: Noctuidae) to Sex Pheromone Compounds (열대거세미나방 성페로몬 성분에 대한 수컷의 촉각 반응)

  • Cho, Jum Rae;Kim, Jeong Hwan;Seo, Bo Yoon;Seo, Meeja;Lee, Gwan Seok
    • Korean journal of applied entomology
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    • v.60 no.4
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    • pp.363-367
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    • 2021
  • This study was conducted to investigate the EAG (electroantennogram) response of Spodoptera frugiperda male to sex pheromone compounds and whether or not S. frugiperda male adults would undergo double mating. The EAG response of S. frugiperda male adult to Z9-14:Ac increased in a dose-dependent as the dose increased. Among the 7 sex pheromone components investigated, male EAG recording was the highest to Z9-14:Ac. The EAG response of S. frugiperda male adult to the mixed sex pheromone component was greater than that to the single component. Male adults of S. frugiperda were capable of double mating under laboratory condition, and the secondary mating rate increased to 72.2% compared to the 58.3% of primary mating rate. The EAG response of mated S. frugiperda male adult was not different from that of unmated S. frugiperda male. In the net house test with sex pheromone lure, mated male adults were not captured during the test period. Also, strangely, unmated male adults were not captured even in a trap equipped with virgin female adults, although the antennae of mated male adult were responded to the sex pheromone component in the laboratory. Probably, it is thought that the mated male adults may not have been caught in the trap be due to flight ability which has been decreased after mating. The field attractiveness of S. frugiperda male adults to sex pheromones remains to be further elucidated.

Effects of olfactory self- and cross-adaptation on perceiving odor in a moth

  • Qian, Kai;Chen, Haibin;Wan, Xinlong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.32 no.2
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    • pp.60-68
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    • 2016
  • Pheromone orientation in moths is an exemplar of olfactory sensitivity. To avoid cross mating, the responses of males to pheromone blends must be high specificity and temporal resolution. We tested the effects of olfactory self- and cross-adaptation of pheromone compounds and mixtures in Spodoptera litura moths by electroantennogram (EAG) recordings. The challenge of S. litura antennae to a pulse train of its own pheromone blends of Z9,E11-14:OAc and Z9,E12-14:OAc with 200 ms on/off and 1 s on/off indicated that the repetitive stimulation by 200 ms on/off with high dosages resulted in greater adaptation than that by 1 s on/off with low dosages and the adaptation index of Z9,E11-14:OAc in all treatments is significantly larger than that of Z9,E12-14:OAc, suggesting that high dosages with more frequent stimulation prefer to induce sensory adaptations and a different odor coding exist between the two components in the antennal periphery in this moth. The cross-adaptation EAG test among the two pheromone compounds and Z7-12:OAc and Z9-14:OH from congeneric species of S. litura showed that each of these compounds adapted the antenna more to that specific compound. The significantly higher adaptation to Z7-12:OAc and Z9-14:OH than to the pheromone components of S. litura induced by themselves suggested that both of them are coded by specific odor receptor neurons which are different from those tuned to the pheromone components of S. litura . Thus, we proposed that Z7-12:OAc and Z9-14:OH may play an important role in avoidance of heterospecific mating between S. litura and its sympatric moth species.

Correctum : Effects of olfactory self- and cross-adaptation on perceiving odor in a moth

  • Qian, Kai;Chen, Haibin;Wan, Xinlong
    • International Journal of Industrial Entomology and Biomaterials
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    • v.33 no.2
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    • pp.149-149
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    • 2016
  • Pheromone orientation in moths is an exemplar of olfactory sensitivity. To avoid cross mating, the responses of males to pheromone blends must be high specificity and temporal resolution. We tested the effects of olfactory self-and cross-adaptation of pheromone compounds and mixtures in Spodoptera litura moths by electroantennogram (EAG) recordings. The challenge of S. litura antennae to a pulse train of its own pheromone blends of Z9,E11-14:OAc and Z9,E12-14:OAc with 200 ms on/off and 1 s on/off indicated that the repetitive stimulation by 200 ms on/off with high dosages resulted in greater adaptation than that by 1 s on/off with low dosages and the adaptation index of Z9,E11-14:OAc in all treatments is significantly larger than that of Z9,E12-14:OAc, suggesting that high dosages with more frequent stimulation prefer to induce sensory adaptations and a different odor coding exist between the two components in the antennal periphery in this moth. The cross-adaptation EAG test among the two pheromone compounds and Z7-12:OAc and Z9-14:OH from congeneric species of S. litura showed that each of these compounds adapted the antenna more to that specific compound. The significantly higher adaptation to Z7-12:OAc and Z9-14:OH than to the pheromone components of S. litura induced by themselves suggested that both of them are coded by specific odor receptor neurons which are different from those tuned to the pheromone components of S. litura. Thus, we proposed that Z7-12:OAc and Z9-14:OH may play an important role in avoidance of heterospecific mating between S. litura and its sympatric moth species.

Biological Activity of Female Sex Pheromone of the Oriental Tobacco Budworm, Helicoverpa assulta (Guenee)(Lepidoptera: Noctuidae): Electroantennography, Wind Tunnel Observation and Field Trapping (담배나방 성페르몬의 생물학적 활성 : 촉가전도, 풍동 및 애외 트랩시험)

  • 박계청;알란콕;부경생;데이빗홀
    • Korean journal of applied entomology
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    • v.33 no.1
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    • pp.26-32
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    • 1994
  • Electroantennography, wind tunnel observation, and field trapping experiments were carried out to investigate the biological activity of synthetic sex pheromone in the onental tobacco budworm. Heli-couerpa assulta. Two major sex pheromone components of H. assulta, Z9-16' Ald and Z11-16: Ald. elicited a big EAG response in male, but not in female Their mIXture ratios did not give much influence on EAG size Fema]e H assulta showed a great EAG response only to its host plant e extract. EAG size also increased with the amount of mixture from 001 to 10 [lg but rather decreased w when the amount was 1 00 $\mug$. H. assulta always revealed a series of stereotyped behavior in a wind tunnel. The behavioral response was different when the males were stimulated with the sex pheromone containing some minor components, 16: AId and Z9.16: Ac, or being different in mixing ratios of the two major components. The best ratio of the sex phemmone components for a attracting H assulta male adults was 20-25: 1 between Z9-16: Aid and Z11-16: Ald in net house a and red pepper field experiments in Korea When the lure contamed Z9-16: OH, attracting power rapidly decreased. The synthetic sex pheromone showed a strong attraction when compared to virgin females

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