Characteristics of Electroantennogram Parameters for the Detection of Odorants

  • Yun, Eung-Sik (School of Chemical Engineering, Seoul National University) ;
  • Ko, Hwi-Jin (School of Chemical Engineering, Seoul National University) ;
  • Sun, Jong-Hwan (School of Chemical Engineering, Seoul National University) ;
  • Park, Tai-Hyun (School of Chemical Engineering, Seoul National University)
  • 발행 : 2000.12.01

초록

Electroantennogram(EAG) can be applied to detect odorants since insects have highly specialized olfactory receptors inside their antennae. The characteristics of EAG parameters were investigated for the quantitative measurement of a general odorant using ammonia as a model odorant. The antennae of male silkworm moth, Bombyx mori, were used for the EAG. The electical signal curves generated from a pair of antennae originating from the same silkworm moth were never identical; however, they exhibited a typical type I or type II characteristic curve pattern for every pair of antennae. The correlation between the EAG parameters and the ammonia concentration was analyzed for the type I and type II antennae. The stability of each parameter was also investigated for each type of antenna. The results show the possibility of the quantitative measurement of general odorants using the EAG technique.

키워드

참고문헌

  1. J. Insect Physiol v.39 Detection and deactivation of pheromone and plant odor components by the beet armyworm, Spodoptera exigua(Hubner) (Lepidoptera: Noctuidae) Dickens, J. C.;J. H. Visser;J. N. C. van der Pers
  2. Trends Biotechnol v.16 Current trends in 'artificial-nose' technology Dickinson, T. A.;J. White;J. S. Kauser;D. R. Walt
  3. Cell biology of olfaction Farbman, A. I.
  4. Annu. Rev. Physiol. v.44 Chemotaxis in Dictyostelium Gerisch, G.
  5. J. Microbiol. Biotechnol. v.8 Expression and characterization of recombinant E2 protein of hepatitis C virus by insect cell/baculovirus expression system Han, B. K.;B. Lee;M. K. Mil;K.-H. Jung
  6. J. Microbiol. Biotechnol. v.8 cDNA cloning and expression of human rotavirus outer capsid protein VP7 in insect cell Kang, D. K.;K. W. Kim;P.-H. Kim;S. Y. Seoung;Y. H. Kim;I. C. Kwon;s. Y. Jeong;E.-Y. Choi;K. M. Lee;H. S. Kim;E. C. Kim;S. I. Joo;J. M. Yang
  7. J. Microbiol. Biotechnol. v.9 Reduction of FBS concentration through adaptation process mammalian cell culture and addition of silkworm hemolymph in insect cell culture Kim, E. J.;T. H. Park
  8. J. Microbiol. Biotechnol. v.8 Construction of a baculovirus expression system using Hyphantria cunea nuclear polyhedrosis virus for eukaryotic cells Lee, H. H.;B. J. Kang;K. J. Park;S. C. Cha
  9. J. Microbiol. Biotechnol. v.9 Construction of a transfer vector containing the gX signal sequence of pseudorabies virus and a recombinant baculovirus Lee, H. H.;H. Kang;J. W. Kim;S. K. Hong;B. J. Kang;J. Y. Song
  10. J. Microbiol. Biotechnol. v.8 Construction of a baculovirus Hyphantria cunea NPV insecticide containing the insecticidal protein gene of Bacillus thuringiensis subsp. kurstaki HD1 Lee, H. H.;E. S. Moon;S. T. Lee;S. H. Hwang;S. C. Cha;K. H. Yoo
  11. J. Chem. Ecol. v.16 Introduction to bacterial motility and chemotaxis Manson, M. D.
  12. Science v.286 Seven-transmembrane proteins as odorant and chemosensory receptors Mombaerts, P.
  13. J. Microbiol. Biotechnol. v.9 Biochemical analysis of Anagrapha falcifera NPV atachment to Spodoptera frugiperda 21 cells Park, J. O.;J. M. Yang;I. S. Chung
  14. Kor. J. Biotechnol. Bioeng v.13 Smell perception process and olfactory sensor Park, T. H.;E. S. Yun
  15. J. Chem. Ecol. v.16 Pheromone interactions and ionic communication on gametes of aquatic fungus Allomyces macrogynus Pommerville G. C.;J. B. Strickland;K. E. Garding
  16. Chem. Senses v.17 A portable EAG system for measurement of pheromones concentrations in the field Sauer, A. E.;G. Karg;U. T. Koch;J. J. Kramer;R. Milli
  17. Ann. Rev. Microbiol. v.37 Cell interactions and regulation of cell type in the yeast Sacchsaromyces cerevisiae Sprague, G. F.;L. C. Blair;J. Thorner
  18. Bombyx mori. Biotechnol. Bioprocess Eng. v.5 Quantitative measurement of general odorant using electroantennogram of male silkworm moth Yun, E. S.;T. H. Park