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http://dx.doi.org/10.4014/jmb.2006.06022

Apoptosis in Leukemic Cells Induced by Anti-Proliferative Coumarin Isolated from the Stem Bark of Fraxinus rhynchophylla  

Lee, Beom Zoo (Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University)
Lee, Ik Soo (Herbal Medicine Research Division, Korea Institute of Oriental Medicine)
Pham, Chau Ha (Group for Biometrology, Korea Research Institute of Standards and Science (KRISS))
Jeong, Soon-Kyu (Chemland Co., Ltd.)
Lee, Sulhae (Chemland Co., Ltd.)
Hong, KwangWon (Department of Food Science and Biotechnology, College of Life Science and Biotechnology, Dongguk University)
Yoo, Hee Min (Group for Biometrology, Korea Research Institute of Standards and Science (KRISS))
Publication Information
Journal of Microbiology and Biotechnology / v.30, no.8, 2020 , pp. 1214-1221 More about this Journal
Abstract
Esculetin 6-O-β-D-arabinofuranosyl-(1 → 6)-β-D-glucopyranoside (EAG) is a coumarin glycoside isolated from the stem bark of Fraxinus rhynchophylla. This study scrutinized the anti-proliferative activity of EAG on blood cancer-derived Jurkat leukemic cells. Cell viability assays in leukemic cancer cells determined that EAG possesses potent anti-proliferative effects. Moreover, treatment with EAG increased the proportion of apoptotic cells, resulted in cell cycle arrest being induced at the subG0/G1 phase, and reduced the proportion of cells present in the S phase. In addition, mitochondrial membrane potential was reduced by EAG in Jurkat cells. Additionally, EAG triggered apoptosis that was mediated by the downregulation of BCL-XL, p-IκBα, and p-p65 expressions in addition to the upregulation of cleaved Caspase 3 and BAX expressions. These findings revealed that the toxic effect of EAG was mediated by intracellular signal transduction pathways that involved a mechanism in which reactive oxygen species (ROS) were upregulated. Thus, this study concludes that EAG could potentially serve as a therapeutic agent for leukemia.
Keywords
Fraxinus rhynchophylla; coumarin; leukemia; apoptosis; ROS; cytotoxicity;
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