• 한국어류학회지
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• 제9권2호
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• pp.195-201
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• 1997

수온 및 estradiol-17 ($E_2$) 처리가 나일틸라피아의 성결정에 미치는 효과를 조사하기 위하여 세가지의 유전자형 집단(XX : XY=1 : 1, 1 : 0, 0 : 1)을 대상으로 실험을 실시하였다. 실험군들간 수온 및 $E_2$ 처리에 따른 생존율의 유의한 차이는 없었다. 사육 수온에 따른 성비는 대조군($27^{\circ}C$)에서는 각 유전자형에 따라 예상되는 암수 비율이 관찰되었다. 그러나 성분화 시기에 사육 수온을 33 및 $36^{\circ}C$로 증가시킨 실험군에서는 암컷의 비율이 유의적으로 높았으며, $33^{\circ}C$에 비해 $36^{\circ}C$ 실험군의 암컷률이 높았다. 서로 다른 처리 수온 조건에서 $E_2$ 처리를 병행한 결과 모든 처리 수온에서 호르몬 처리를 하지 않은 실험군들보다 유의적으로 높은 암컷률을 보였다.

• The Korean Journal of Physiology and Pharmacology
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• 제6권5호
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• pp.261-267
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• 2002

The aim of the present study was to investigate the interaction of $estradiol-17{\beta}-bovine$ serum albumin $(E_2-BSA)$ and calcitropic hormones, such as parathyroid hormone, calcitonin, and vitamin D, in regulation of $Ca^{2+}$ uptake in primary cultured renal proximal tubule cells. Statistically significant increase in $Ca^{2+}$ uptake was found from 2 hours after $(E_2-BSA)\;(10^{-9}\;M)$ treatment, while $estradiol-17{\beta}\;(10^{-9}\;M)$ did not affect. Treatment of the cells with $(E_2-BSA)\;(10^{-9}\;M)$ together with parathyroid hormone (PTH) $(10^{-8}\;M),$ vitamin D $(10^{-8}\;M),$ or calcitonin $(10^{-8}\;M)$ significantly stimulated $Ca^{2+}$ uptake by 32.50%, 29.30%, or 27.75%, respectively, compared with the control. However, calcitropic hormones did not exhibit any synergistic effect on the E2-BSA-induced stimulation. $E_2-BSA$ significantly increased cAMP generation and PKC activity. The stimulatory effect of cotreatment of $E_2-BSA$ and PTH or vitamin D was blocked by SQ22536 (an adenylate cyclase inhibitor) and staurosporine (a PKC inhibitor), but the effect of cotreatment of $E_2-BSA$ and calcitonin was not blocked. Furthermore, 8-Br-cAMP and TPA (an artificial PKC promoter) increased $Ca^{2+}$ uptake by 25.51% and 16.47%, respectively, compared with the control. In conclusion, $E_2-BSA$ combined with calcitropic hormones regulated $Ca^{2+}$ uptake partially via cAMP and PKC-dependent mechanisms in renal proximal tubule cells.

• 한국가축번식학회지
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• 제22권1호
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• pp.29-34
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• 1998

본 연구는 한국재래산양 16두를 공시재료로 사용하여 분만전 12일부터 분만후 1일까지 경정맥의 혈장중 luteinizing hormone, prolactin, estradiol-17${\beta}$ 및prostaglandin F$_2$의 농도를 측정한 결과는 다음과 같다. 1, 한국재래산양에서 혈장중 luteinizing hormone외 농도는 분만전 12일부터 분만후 1일까지 0.20${\pm}$0,02~0,38${\pm}$0.04 mIU/ml의 범위로서 비교적 일정한 농도를 보였으나 prolactin의 농도는 분만전 12일부터 약간 증가하는 경향을 보였다. 2, 분만 전, 후의 estradiol-17${\beta}$의 농도는 분만전 1일에 급증하여 분만시 784.8${\pm}$77.5 pg/ml로서 최고치에 도달하였으며 분만후 1일에는 63.8${\pm}$2.8pg/ml로 감소하였다. 3. 분만 전, 후의 prostaglandin F$_2$의 농도는 분만전 12일에 323.2${\pm}$69.6 Pg/ml로 분비가 시작하여 분만 전일에 증가하였으며 분만시 1081,4${\pm}$164,9pg/ml로서 절정에 도달하여 분만후 1일에 PGF$_2$의 농도는 425.3${\pm}$60.4pg/ml로서 감소하였다. 이상의 결과는 재래산양의 분만에 있어서 estradiol-17${\beta}$의 농도에서 증가와 연관하여 prostaglandin F$_2$의 농도는 점차적으로 증가되는 것으로 추정되었다.

• 한국어류학회지
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• 제12권3호
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• pp.173-179
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• 2000

Estradiol-$17{\beta}(E_2)$와 2,4-dichlorophenoxy acetic acid (2, 4- D)가 난황 전구물질의 합성에 미치는 영향을 넙치 Paralichthys olivaceus 간세포의 초대 배양을 통하여 조사하였다. 배양간세포의 생존율은 배양온도 $27^{\circ}C$에 서 가장 높게 나타났으며, $15^{\circ}C$에서는 생존율이 급격히 감소하여 약 50%의 생존율을 나타내었다. $E_2$에 의한 VTG 의 합성은 $10^{-6}M$에서 최대치를 나타내었다. 2, 4-D $10^{-7}\sim10^{-5}M$의 첨가에 의해 VTG의 합성은 이루어지지 않았다. 그러나, 저농도인 $10^{-8}M$에서는 VTG의 합성이 증가하였다. $E_2$ 및 2, 4-D에 의해 합성된 VTG는 $10^{-6}M$ tamoxifen의 첨가에 의해 유의하게 억제되었다 (P<0.01). 본 연구 결과에서 $E_2$와 2, 4-D의 동시 첨가는 VTG의 합성을 억제하지 않았다. 이러한 결과는 2, 4-D의 작용이 $E_2$와 유사한 작용을 가지지만, VTG의 합성에 있어서 $E_2$ 수용체에의 작용 양식은 서로 다른 것으로 추정된다.

• Fisheries and Aquatic Sciences
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• 제4권4호
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• pp.186-191
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• 2001

Vitellogenin (VTG) and VTG mRNA induction by $estradiol-17\beta\;(E_2)$ were examined in the primary cultures of hepatocyte in the rainbow trout. Hepatocytes were precultured for 2 days, then $E_2$ was added and cultured for another 5 days. Media and hepatocytes were then analyzed by electrophoresis and Northern blotting for VTG and VTG mRNA, respectively. The hepatocytes were formed a few aggregates within 5 days without further spreading to a monolayer. Cell viability and high DNA content were maintained during the incubation. The hepatocyte culture with E2 induced a weak VTG band at a molecular weight of 175kDa on Day 2 after $E_2$ addition. The relative amount of VTG was expressed in percentage of total protein concentrations. VTG was gradually increased as $1.9\%$ on Day 2, $6.3\%$ on Day 4 and $7.3\%$ on Day 5. VTG mRNA band was detected at about 6.6 kb in the culture with $E_2$ at day 1 of culture. The level of VTG mRNA expression linearly increased with time until Day 5 (r=0.97).

• 대한의생명과학회지
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• 제23권1호
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• pp.17-24
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• 2017

Estrogens are effective neuroprotectants in vivo and in vitro. To obtain a better insight into the molecular mechanisms of action of neuroprotection by $17{\beta}-estradiol$ (E2), we examined the differential effects of E2 on the fluidity of synaptosomal plasma membrane vesicles (SPMV) isolated from rat cerebral cortex. Intramolecular excimerization of 1,3-di(1-pyrenyl)-propane (Py-3-Py) was used to investigate the effects of E2 on the bulk and annular lateral diffusion of the SPMV. In addition, we examined the effects of E2 on the rotational diffusion of individual leaflet of SPMV exploiting selective quenching of outer monolayer 1,6-diphenyl-1,3,5-hexatriene (DPH) fluorescence by trinitrophenyl groups. The $F{\ddot{o}}rster$ distance $R_0$ value for the tryptophan-Py-3-Py donor-acceptor pair was $26.9{\AA}$. E2 increased the lateral mobility of both bulk and annular lipids in SPMV in a dose-dependent manner, but a larger effect on bulk lipids was observed. Although E2 decreased the anisotropy of DPH in SPMV, E2 had a greater fluidizing effect on the outer leaflet compared to the inner leaflet. These results suggest that E2 selectively fluidizes the more fluid regions within SPMV. It is highly probable that E2 mostly fluidizes the bulk lipids, away from either annular lipids or lipid rafts, in the outer leaflet of SPMV. This selective fluidization may be one of the nongenomic mechanisms of neuroprotection by E2.

• Maxillofacial Plastic and Reconstructive Surgery
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• 제22권2호
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• pp.155-163
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• 2000

Osteoporosis is the consequence of an imbalance between osteoclastic and osteoblastic activity, coupled with an increased rate of bone turnover observed with menopause. Estrogen is generally considered to maintain bone mass through suppression of bone resorption. The purpose of this study was to evaluate the rat femoral trabecular change not only in the deficiency of estrogen but also in the administration of estrogen following ovariectomy(OVX). 30 female Sprague-Dawley rats were subjected to bilateral OVX or sham surgery(control). Groups of OVX were divided into 4 groups. The first group was injected daily with vehicle alone for 20 days after 20 weeks following OVX. The additional groups of OVX was injected daily with low, medium, or high doses of $17{\beta}-estradiol$(10, 25 or $50{\mu}g/kg$ BW, respectively). All rats were sacrified 23 weeks after OVX, and their femur were processed for H&E, MT stain and histomorphometry. The results were as follows; 1. In the histomorphometric analysis, the trabecular bone volume/tissue volume, trabecular thickness and trabecular seperation were respectively $31.2{\pm}8.3%$, $54.3{\pm}4.8{\mu}m$ and $280.7{\pm}16.4{\mu}m$ in vehicle treated OVX group and $48.6{\pm}7.3%$, $90.4{\pm}4.5{\mu}m$ and $126.3{\pm}5{\mu}m$ in sham operation group, and they showed statistical significance compare to control group. 2. The trabecular bone volume/tissue volume, trabecular thickness and trabecular separation were respectively $44.4{\pm}4.3%$, $109.5{\pm}12.3{\mu}m$ and $94.9{\pm}8.5{\mu}m$ in low doses of $17{\beta}-estradiol$ injected group and they showed statistical significance compare to OVX group. 3. The trabecular bone volume/tissue volume, trabecular thickness and trabecular separation were respectively $44.4{\pm}4.3%$, $109.5{\pm}12.3{\mu}m$ and $94.9{\pm}8.5{\mu}m$ in medium doses of $17{\beta}-estradiol$ injected group and they showed statistical significance compare to OVX group, but they didn't show statistical significance compare to low doses of $17{\beta}-estradiol$ injected group. 4. The trabecular bone volume/tissue volume, trabecular thickness and trabecular separation were respectively $46.4{\pm}4.5%$, $154.4{\pm}13.2{\mu}m$ and $113.7{\pm}12.8{\mu}m$ in high doses of $17{\beta}-estradiol$ injected group and they also showed statistical significance compare to OVX group, but they didn't show statistical significance compare to other experimental groups. From the above results, metaphyseal bone formation was markedly reduced in OVX rate but treatment of OVX rats with $17{\beta}-estradiol$ resulted in normalization of femur trabecular bone volume. But they didn't show statistical significance the effect of bone formation according to the dose dependency.

• 한국양식학회지
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• 제9권3호
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• pp.279-285
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• 1996

넙치의 전 암컷 집단의 생산을 위한 연구의 일환으로 실제 양식 어민이 손쉽게 이용할 수 있는 성전환 처리방법을 개발하고자, 부화 후 30일째부터 90일까지 60일간 $17{\beta}-methyltestoste-rone$ (MT)과 $estradiol-17{\beta}\;(E_2)$을 $1\~100$ ppb로 매일 2시간 동안 침지하여 성전환을 유도하였다. 부화 후 200일째의 실험군의 성비는 대조군에서 수컷이 $46.7\%$로 나타나 암수비가 거의 1 : 1이었으나, MT에 의한 수컷 유도율은 1 ppb 처리군이 $72.5\%$, 그리고 10 ppb 처리군이 $87.2\%$로 나타났고, 특히 100 ppb 처리군의 경우 $100\%$로 나타났다. $E_2$에 의한 암컷 유도율은 1 ppb 처리군에서 $75.5\%$ ,10 ppb 처리군에서 $91.9\%$였고, 가장 높은 농도인 100 ppb에서는 $97.2\%$로 나타났다. 호르몬 농도에 따른 각 처리군별 생존율 및 성장은 유의한 차이가 없었다(P>0.05).

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2005년도 생물공학의 동향(XVI)
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• pp.88-92
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• 2005

Aptamer is the single-stranded oligonucleotide which binds to various target molecules such as proteins, peptides, lipids and small organic molecules with high affinity and specificity. DNA aptamers specific for the $17{\beta}-estradiol$ were selected by SELEX (Systematic Evolution of Ligands by EXponential enrichment) process from a random DNA library. These DNA aptamers have a high affinity to $17{\beta}-estradiol$ as an endocrine disrupting chemical. Nanowell and $200{\mu}m$ gold electrode were used as substrate for DNA aptamer immobilization and electrochemical analysis. Especially, nanowell gold electrode was fabricated by e-beam lithography. The size of single nanowell is 130nm and 40,000 nanowells were deposited on one gold electrode. The immobilization method was based on the interaction between the biotinylated aptamer and streptavidin deposited on gold electrode previously. Immobilization procedure was optimized by surface plasma resonance (SPR) and electrochemical analysis. After the immobilization of DNA aptamer on streptavidin modified gold electrode, $17{\beta}-estradiol$ solution was treated on aptamer immobilized gold electrode. The current of gold electrode was decreased by the binding of $17{\beta}-estradiol$ to DNA aptamer immobilized on gold electrode. However, in negative control experiments of 1-aminoanthraquinone and 2-methoxynaphthalene, the current was rarely decreased. And more sensitive data was obtained from nanowell gold electrode comparing with $200{\mu}m$ gold electrode.

• 생명과학회지
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• 제19권12호
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• pp.1851-1854
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• 2009

본 연구는 통영 해역에서 채집되어진 갯장어, Muraenesox cinereus의 생식년주기에 있어서 난소 발달과 생식소중량지수 (gonadosomatic index; GSI) 변화에 따른 혈중 estradiol- $17{\beta}$ ($E_2$) 농도 변화를 서로 연관시켜 조사하였다. 난소 성숙도는 조직학적 관찰을 기초로 주변인기(11월-2월), 유구기(3월-4월), 초기 난황형성기(4월-5월) 및 후기 난황형성기(6월-10월)로 나 눌 수 있었다. GSI의 계절적 변화는 수온과 밀접한 연관성을 가지고 있으며, 난소의 성숙 정도에 영향을 미쳤다. 혈중 $E_2$ 농도는 GSI 변화와 관련이 있어 4월에 증가하여 7월에 년중 최고치에 도달하였고 10월까지 비교적 높은 농도로 유지하였다. 이러한 결과들은 GSI와 혈중 $E_2$ 농도 변화는 갯장어 난소 발달과 밀접한 관련이 있음을 나타내고 있다. 그러나 본 연구에서는 산란기의 암컷 갯장어를 채집하지 못하였다. 이러한 이유로 산란기의 개체는 외해의 심해쪽으로 이동하기 때문이라고 추측된다.