• Title/Summary/Keyword: E171

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Cloning of the rbcL Gene from Maize Chloroplast (옥수수 엽록체 rbcL 유전자의 클로닝)

  • 이재선
    • Journal of Plant Biology
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    • v.35 no.2
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    • pp.165-171
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    • 1992
  • In order to study regulation of rbcL gene expression, rbcL gene of chloroplast DNA (Cp DNA) from maize was cloned. Cp DNA was isolated from intact chloroplast and digested with BamHI. BamHI 9 fragment of Cp DNA containing rbcL gene was ligated to pUC19 and transformed into E. coli DH5a. This recombinant plasmid was named pRLYSl. pRLYSl was hybridized with a part of rbcL gene from rice and digested with restriction enzyme BamHI, HindIIl, and PstI. From these results, it was confirmed that pRLYS1 contains intact rbcL gene and orientation of BamHI 9 fragment of Cp DNA in pRLYS1 was determined.rmined.

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Effects of ChenWhangBosimDan(CWBD) on Inhibition of Impairment of Learning and Memory, and Acetylcholinesterase in Amnesia mice (천왕보심단(天王補心丹)이 치매병태모델에 마치는 영향(影響))

  • Jung, In-Chul;Lee, Sang-Ryong;Lee, Jun-Young
    • Journal of Oriental Neuropsychiatry
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    • v.13 no.2
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    • pp.149-171
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    • 2002
  • Alzheimer's disease(AD) is a progressive neurodegenerative disease, which is pathologically characterized by neuritic plaques and neurofibrillary tangles associated with the acetylcholinesterase, apolipoprotein E and butylcholinesterase, and by mutations in the presenilin genes PS1 and PS2, and amyloid precursor proteins (APPs)'s overexpression. The present research is to examine the inhibitory effect of CWBD on PS1, PS2 and APPs's overexpression detected by Western blotting. To verify further the effects of CWBD on cognitive deficits, we tested it on the scopolamine(1mg/kg)-induced amnesia model of the mice using the Morris water maze tests, and there were ameliorative effects on memory impairment as a protection from scopolamine. CWBD only partially blocked the increase in blood serum level of acetylcholinesterase and Uric acid induced by scopolamine, whereas blood glucose level was shown to attenuate the amnesia induced by scopolamine and increased extracellular serum level. In conclusion, studies of CWBD that has been known as anti-choline and inhibitory ablilities of APPs's overexpression could also be used further as a important research data for a preventive and promising symptomatic treatment for Alzheimer's disease.

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Study of the formation of Pd-silicide with x-ray photoelectron spectroscopy (광전자분광법을 이용한 Pd-실리사이드의 형성 연구)

  • 조은진;최일상;이한길;황찬용
    • Journal of the Korean Vacuum Society
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    • v.6 no.2
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    • pp.165-171
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    • 1997
  • If the thickness of Pd deposited is larger than 9$\AA$, its phase is $Pd_3Si$. This phase is followed by pure Pd phase with further deposition of Pd. Also, when the thickness of Pd deposited on top of Si(111) is larger than 1$\AA$, the phase of Pd-silicide formed is found to be Pd2Si. The full width at half maximum of Pd 3d core-levels increases with decreasing of Pd film thickness at low coverages ($\leq0.5\AA$). This is due to the formation of additional phase of Pd silicide, i.e. PdSi, in addition to $Pd_2Si$.

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Creep Behavior of Press Joined Molding GMT-Sheet (프레스 접합성형 GMT-Sheet의 크리프 특성)

  • Choi, Yu-Seong;Kim, Hyuk;Kang, Myoung-Goo;Lee, Dong-Gi;Han, Gil-Young;Kim, E-Gon
    • Proceedings of the Korea Committee for Ocean Resources and Engineering Conference
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    • 2000.10a
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    • pp.171-177
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    • 2000
  • It is essential to understand the creep behavior, which shows how long the characteristics of material maintains because press joined molding GMT-Sheet for recycle is usually used in the severe environment. In this study, we predict joining strength of GMT-Sheet for recycle, when lap length was changed. and we will investigate how compression ratio have an effect on creep behavior in press joined molding. The result of experiment of forming condition concerned with joining problem of GMT-Sheet is as followings joining efficiency. of GMT-Sheet, increases as lap joint length I, increases. Increase of compression ratio causes decrease o f joining efficiency after of GMT-Sheet joining. As the result of creep test, GMT-Sheet is easily damaged in high temperature range, because it is sensitive to the temperature

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Changes in the Attitudes and Behavior of Relatives of Breast Cancer Patients Concerning Cancer Prevention and Screening

  • Koca, Dogan;Ozdemir, Oguzhan;Akdeniz, Huseyin;Unal, Olcun Umit;Yilmaz, Ugur
    • Asian Pacific Journal of Cancer Prevention
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    • v.14 no.10
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    • pp.5693-5697
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    • 2013
  • Background: Changes in the attitudes and behavior of relatives of breast cancer patients concerning cancer prevention and screening after diagnosis in a loved one were evaluated. Materials and Methods: Forty-three questions were used to collect data from the relatives of the breast cancer patients who had been living with their relatives for at least one year. Results: The study group was composed of 171 female relatives (median age: 43, range: 17-82 yr). After the patients were diagnosed with breast cancer, changes in the attitudes and behavior of their relatives toward the prevention and screening of cancer were evident in 78 (45.6%) of the study participants (e.g. eating habits, quit or reduced smoking, exercise habits). In addition, it was noted that some characteristics of the relatives had different effects on different attitudes and behavior. Conclusions: Awareness on breast cancer among the relatives of breast cancer patients is useful for the management of health and social problems that can be seen in these individuals. At the same time, this information could help countries determine whether their actual level of healthcare for early cancer diagnosis, prevention, and screening are adequate.

Implications in Case Study on the Electronic Bills of Lading (전자선화증권의 기능에 대한 사례분석과 시사점)

  • Choi, Seok-Beom;Kim, Tae-Hwan
    • International Commerce and Information Review
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    • v.7 no.1
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    • pp.171-194
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    • 2005
  • The CMI Rules for Electronic Bills of Lading were drafted by the Comite Maritime International(CMI) and related specifically to EDI transfers of negotiable bills of lading. The CMI Rules are contractual in nature and the application of the CMI Rules is strictly voluntary. The method adopted in the CMI Rules to enable negotiation of the rights to cargo in a controlled method is based on the introduction of the ‘Private Key’. The Bolero service will be governed by a multilateral contract called the Bolero Rule Book which specifies the rights and responsibilities of Bolero and its users. The Title Registry and Bolero Bill of Lading provide a fully functional equivalent to the paper bill of lading. The Bolero Bill of Lading can be created, transferred, amended, and surrendered by way of designating to order party, blank endorsement, refusal by the transferee etc. In Japan, through TEDI Project, the RSP Model is introduced as e-trade solution like Bolero's Solution. The RSP Model Solution will be furnished through TC(Trade Chain) Server and RSP(Repository Service Provider) Server. The purpose of this paper is to analyze comparatively the CMI Rules and Bolero Bill of Lading and RSP Model of TEDI and to find the implications in this case study for quick introduction of electronic Bill of Lading.

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Characterization of nucleotide-induced changes on the quaternary structure of human 70 kDa heat shock protein Hsp70.1 by analytical ultracentrifugation

  • Borges, Julio C.;Ramos, Carlos H.I.
    • BMB Reports
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    • v.42 no.3
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    • pp.166-171
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    • 2009
  • Hsp70s assist in the process of protein folding through nucleotide-controlled cycles of substrate binding and release by alternating from an ATP-bound state in which the affinity for substrate is low to an ADP-bound state in which the affinity for substrate is high. It has been long recognized that the two-domain structure of Hsp70 is critical for these regulated interactions. Therefore, it is important to obtain information about conformational changes in the relative positions of Hsp70 domains caused by nucleotide binding. In this study, analytical ultracentrifugation and dynamic light scattering were used to evaluate the effect of ADP and ATP binding on the conformation of the human stress-induced Hsp70.1 protein. The results of these experiments showed that ATP had a larger effect on the conformation of Hsp70 than ADP. In agreement with previous biochemical experiments, our results suggest that conformational changes caused by nucleotide binding are a consequence of the movement in position of both nucleotide- and substrate-binding domains.

Utilizing Advanced Pad Conditioning and Pad Motion in WCMP

  • Kim, Sang-Yong;Chung, Hun-Sang;Park, Min-Woo;Kim, Chang-Il;Chang, Eui-Goo
    • Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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    • 2001.11b
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    • pp.171-175
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    • 2001
  • Chemical mechanical polishing(CMP) process has been widely used to planarize dielectrics and metal, which can apply to employed in integrated circuits for sub-micron technology. Despite the increased use of CMP process, it is difficult to accomplish the global planarization of free-defects in inter level dielectrics and metal. Especially, defects like (micro-scratch) lead to severe circuit failure, and affects yield. Current conditioning method - bladder type, orbital pad motion- usually provides unsuitable pad profile during ex-situ conditioning near the end of pad life. Since much of the pad wear occurs by the mechanism of bladder type conditioning and its orbital motion without rotation, we need to implement new ex-situ conditioner which can prevent abnormal regional force on pad caused by bladder-type and also need to rotate the pad during conditioning. Another important study of ADPC is related to the orbital scratch of which source is assumed as diamond grit dropped from the strip during ex-situ conditioning. Scratch from diamond grit damaged wafer severely so usually scraped. Figure 1 shows the typical shape of scratch damaged from diamond. e suspected that intensive forces to the edge area of bladder type stripper accelerated the drop of Diamond grit during conditioning. so new designed Flat stripper was introduced.

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A Synoptic and Climatological Comparison of Record-breaking Heat Waves in Korea and Europe (한반도와 유럽에서 발생한 폭염의 종관기후학적 특성 비교)

  • Kim, Jiyoung;Lee, Dae-Geun;Kysely, Jan
    • Atmosphere
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    • v.18 no.4
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    • pp.355-365
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    • 2008
  • Synoptic and climatological characteristics of heat waves over Korea and Europe as well as their biometeorological impacts were compared. In July of 1994, excess deaths of about 2,388 in the population of South Korea are estimated by the modified excess death calculation algorithm ofKysely (2004). The excess deaths correspond to the net mortality increase of 12.5% in July of 1994 if we compare the estimated value to the expected number of deaths in this month (i.e., about 19,171). The comparative study of heat waves in Korea and Europe shows that the record-breaking heat waves in both regions are closely associated with prolonged droughts. In particular, reduction of soil moisture, precipitation and cloud cover and enhancement of insolation during the drought periods are very likely to be related to the increase in the intensity and the duration ofheat waves. Climate models predict that the frequency, intensity, and duration of heat waves in the 21 st century will be greatly enhanced in both areas. In order to reduce the biometeorological and socioeconomic impacts due to heat waves, not only the development of heat-related mortality prediction model that can be widely applied to many climate regimes, but also studies on the climatological association between extreme temperatures and abnormal hydrological cycle are needed.

A Study on Web-based Electronic Reference Services of Academic Libraries (대학도서관의 웹기반 전자참고정보 서비스에 관한 연구)

  • 유재옥
    • Journal of the Korean Society for information Management
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    • v.17 no.4
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    • pp.171-186
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    • 2000
  • The slody examined whethcr academe libraries deliver web~hased electronic relerencc services lo remole users. Amo~~g 178 acadclnlc lihraies. only 25.5% prolide reference services of inlroducing eleclronic reference materials and 20.8% o f h electron~c rdel-coce mquq rcwices. However, the use oi electronic access to reference inquuy was weak. One acarlcmic llbrarr reccived on avn-agc 3.2 queslions pcr monlh ovw elecmnic reference inquiries Irom remo~e users. Among the 1,128 questions i-cccived eleclroo~ci~lly, oldy 19.8% were reyardcd as real rekrencc queslmns.

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