• Journal of Gastric Cancer
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• v.23 no.2
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• pp.355-364
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• 2023

Background: There are no clear guidelines to determine whether to perform D1 or D1+ lymph node dissection in early gastric cancer (EGC). This study aimed to develop a nomogram for estimating the risk of extraperigastric lymph node metastasis (LNM). Materials and Methods: Between 2009 and 2019, a total of 4,482 patients with pathologically confirmed T1 disease at 6 affiliated hospitals were included in this study. The basic clinicopathological characteristics of the positive and negative extraperigastric LNM groups were compared. The possible risk factors were evaluated using univariate and multivariate analyses. Based on these results, a risk prediction model was developed. A nomogram predicting extraperigastric LNM was used for internal validation. Results: Multivariate analyses showed that tumor size (cut-off value 3.0 cm, odds ratio [OR]=1.886, P=0.030), tumor depth (OR=1.853 for tumors with sm2 and sm3 invasion, P=0.010), cross-sectional location (OR=0.490 for tumors located on the greater curvature, P=0.0303), differentiation (OR=0.584 for differentiated tumors, P=0.0070), and lymphovascular invasion (OR=11.125, P<0.001) are possible risk factors for extraperigastric LNM. An equation for estimating the risk of extraperigastric LNM was derived from these risk factors. The equation was internally validated by comparing the actual metastatic rate with the predicted rate, which showed good agreement. Conclusions: A nomogram for estimating the risk of extraperigastric LNM in EGC was successfully developed. Although there are some limitations to applying this model because it was developed based on pathological data, it can be optimally adapted for patients who require curative gastrectomy after endoscopic submucosal dissection.

• Korean Journal of Culture and Social Issue
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• v.20 no.4
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• pp.307-328
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• 2014

The purpose of this study is to investigate the influence of parental overprotection on Korean college students' moral behaviors. To test the prediction that overprotected participants will show more immoral behaviors in moral dilemma situations, we measured perceived parental overprotectiveness and morality. Participants were 113 college freshmen. Two types of modified experimental paradigms were used to assess participants on their levels of justice-oriented and prosocial morality. Based on whether they displayed moral behavior (i.e., honest or helping behavior) or not, participants were included in either moral or immoral group. Second, the levels of perceived maternal overprotectiveness and paternal overprotectiveness were assessed using Korean-Parental Overprotection Scale (K-POS) and were compared between moral and immoral group. For justice-oriented morality, the results showed that the immoral group reported a significantly higher level of perceived maternal overprotection compared to the moral group (t = 2.16, p < .05). On the contrast, paternal overprotection was not related to participants' honesty. The results indicate that participants who experienced overprotective parental care are more likely to act immorally in moral dilemma situations dealing with justice. Meanwhile, for prosocial morality, both maternal and paternal overprotection levels did not result in significant difference between two groups. More Implications and limitations were discussed.

• Proceedings of the Korea Water Resources Association Conference
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• 2023.05a
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• pp.13-13
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• 2023

디지털 기술은 오늘날 플랫폼과 디지털 트윈의 기술도입을 통해 현실 세계를 네트워크와 가상세계와의 연결이 통합되어진 가상 현실 세계의 입문 도약이다. 현실에서 가상현실의 사이의 디지털 전환(digital transformation)에는 디지털 기술과 솔루션을 비즈니스의 모든 영역에 통합하는 것이 포함된다. 이러한 디지털 전환의 핵심은 데이터에 관한 것이며, 데이터를 활용하여 가치를 창출하고 고객경험과 비즈니스 영역을 극대화하는 방식을 제공한다. 최적의 데이터를 제공하기 위한 플랫폼과 가상 현실세계 구현을 위한 디지털 트윈의 상호연계 관한 기본 개념은 데이터 수집, 데이터 분석, 데이터 시각화 및 데이터 보고와 같은 데이터 비즈니스이다. 현장 데이터는 디지털 양식을 통해 수집, 기록, 저장된다. 현장 IoT 기반 데이터(사진 및 비디오 매체 등)는 지속적으로 수집되고 종종 다른 데이터베이스에 저장되지만 지리 공간적 위치에 연결되지 않는다. 모든 디지털 발전을 조화시키고 지하수 데이터에서 더 빠른 이해를 도출하기 위해서는 디지털 트윈이 시작되어야 한다. 단일 지하수플랫폼에서 현장 조건을 시각화하고 실시간 데이터를 스트리밍하며, 과거 3D 데이터와 상호작용하여지질 또는 지화학 데이터를 선택적 사용을 위해 지하수 플랫폼과 디지털 트윈이 연계되어야 한다. 데이터를 디지털 정보모델과 연결하면 디지털 트윈에 생명을 불어넣을 수 있지만 디지털 트윈의 가치를 극대화하려면 여전히 데이터 플랫폼 서비스와 전달 방식을 선택해야 한다. 지하수 플랫폼동시성을 갖는 디지털 트윈은 정적 및 동적 데이터를 저장하는 데이터베이스 또는 크라우드 서비스에서 데이터를 가져오는 API(애플리케이션 프로그래밍 인터레이스), 디지털 트윈을 위한 호스팅 공간, 디지털 대상을 구축하는 소프트웨어, 구성 요소 간 읽기/쓰기를 위한 스크립트, chatGPT 및 API를 활용할 수 있다. 이를 통해 수집된 데이터의 실시간 양방향 통신기술인 지하수 플랫폼 기술을 활용하여 디지털 트윈을 적용하고 완성할 수 있고, 이를 지하수 분야에도 그대로 적용할 수 있다. 지하수 분야의 디지털 트윈 기술의 근간은 지하수 모니터링을 위한 관측장치와 이를 활용한 지하수 플랫폼의 구축 및 양방향 자료전송을 통한 분석 및 예측기술이다. 특히 낙동강과 같이 유역면적이 넓고 유역 내 지자체가 많아 이해관계가 다양하며, 가뭄과 홍수/태풍 등 기후위기에 따른 극한 기상이변가 자주 발생하고, 또한 보 및 하굿둑 개방 등 정부정책 이행에 따른 민원이 다수 발생하는 지역의 경우 하천과 유역에 대한 지하수 플랫폼과 디지털 트윈의 동시성 기술적용 시 지하수 데이터에 대한 고려가 반드시 수반되어야 한다.

• Journal of Radiopharmaceuticals and Molecular Probes
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• v.8 no.2
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• pp.53-61
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• 2022

This study aimed to increase the targeting ability against PSMA in cell therapy using metabolic glycoengineering and biorthogonal chemistry and to visualize cell trafficking using PET imaging. Cellular membranes of THP-1 cells were decorated with azide(-N₃) using Ac₄ManNAz by metabolic glycoengineering. Engineered THP-1 cells were conjugated with DBCO-bearing fluorophore (ADIBO-Cy5.5) for 1 h at different concentrations and analyzed by confocal fluorescence microscopy and flow cytometry. For PSAM ligand conjugation to THP-1 cells, Ac₄ManNAz treated THP-1 cells were incubated with DBCO-PSMA ligand (ADIBO-GUL) at a final concentration with 100 µM for 1 h. To evaluate the effect on cell recognition, PSMA ligand conjugated THP-1 cells(as effectors) were co-cultured with PSMA positive 22RV1 (as target cells) at 3 : 1 a effector-to-target cell (E/T) ratio. The interaction between THP-1 and 22RV1 was monitored by confocal fluorescence microscopy. For preparing the radiolabeled THP-1, the cells were treated at the activity of ~ 740 kBq of [⁸⁹Zr]Zr(oxinate)₄/5 × 10⁶ cells. Radiolabeled cells were analyzed for determination of cell-associated radioactivity by gamma counting and viability using MTS assay. In the cytotoxicity assay, THP-1 cells did not have any cytotoxicity even when the Ac₄ManNAz concentration was 100 µM. In confocal microscopy and flow cytometry, THP-1 cells were efficiently labeled ADIBO-Cy5.5 in a dose-dependent manner, and the dose of 100 µM was the optimal concentration for the following experiments. The clusters of PSMA ligand-conjugated THP-1 cells and 22RV1 cells were identified, indicating cell-cell recognition over the cell surface between two types of cells. Cell radiolabeling efficiency was 54.5 ± 17.8%. THP-1 labeled with 0.09 ± 0.03 Bq/cell showed no significant cytotoxicity compared to unlabeled THP-1 up to 7 days. We successfully demonstrated that Ac₄ManNAz treated cells were efficiently conjugated with ADIBO-GUL for preparing the PSMA-targeting cells, and [⁸⁹Zr]Zr(oxinate)₄ could be used to label cells without toxicity. It suggested that PSMA-ligand conjugated cell therapy could be improved cell targeting and be monitored by PET imaging.

• Journal of Wellness
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• v.14 no.2
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• pp.207-223
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• 2019

The purpose This study aimed at proposing class module elements for creativity and convergence and class models for creativity and convergence by integrating content elements by physical activity field(health, challenge, competition, ) for physical education and STEAM. For this, literature review, focus group interview(FGI) and discussions with experts were conducted, and the following study results have been drawn up: First, concerning the class module elements for creativity and convergence, total 11 class module elements in the health field were suggested including detecting risks by posture analysis and analyzing and designing amount of physical activity. Second, total 7 module elements in the challenge field were deduced such as anticipation of obstacles to target achievement and modeling of effective exercise. There were 17 convergence elements in the competition field including game record analysis and creation of game data storage application. Third, total 9 creativity and convergence module elements in the field include modeling of technology improvement for motion and symbolization for motion records. In addition, class modules related to convergence with engineering in the health field, convergence with technology in the challenge field, convergence with art in the competition field and convergence with art and mathematical symbols were proposed.

• Proceedings of the Korean Society of Medical Physics Conference
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• 2003.09a
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• pp.44-44
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• 2003

As a basic measurement tool in the areas of animal models of human disease, gene expression and therapy, and drug discovery and development, small animal PET imaging is being used increasingly. An ideal small animal PET should have high sensitivity and high and uniform resolution across the field of view to achieve high image quality. However, the combination of long narrow pixellated crystal array and small ring diameter of small animal PET leads to the degradation of spatial resolution for the source located at off center. This degradation of resolution can be improved by determining the depth of interaction (DOI) in the crystal and by taking into account the information in sorting the coincident events. Among a number of 001 identification schemes, dual layer phsowich detector has been widely investigated by many research groups due to its practicability and effectiveness on extracting DOI information. However, the effects of each crystal length composing dual layer phoswich detector on DOI measurements and image qualities were not fully characterized. In order to minimize the DOI effect, the length of each layer of phoswich detector should be optimized. The aim of this study was to perform simulations using a simulation tool, GATE to design the optimum lengths of crystals composing a dual layer phoswich detector. The simulated small PET system employed LSO front layer LuYAP back layer phoswich detector modules and the module consisted of 8${\times}$8 arrays of dual layer crystals with 2 mm ${\times}$ 2 mm sensitive area coupled to a Hamamatsu R7600 00 M64 PSPMT. Sensitivities and variation of radial resolutions were simulated by varying the length of LSO front layer from 0 to 10 mm while the total length (LSO + LuYAP) was fixed to 20 mm for 10 cm diameter ring scanner. The radial resolution uniformity was markedly improved by using DOI information. There existed the optimal lengths of crystal layers to minimize the variation of radial resolutions. In 10 cm ring scanner configuration, the radial resolution was kept below 3.4 mm over 8 cm FOV while the sensitivity was higher than 7.4% for LSO 5 mm : LuYAP 15 mm phoswich detector. In this study, the optimal length of dual layer phoswich detector was derived to achieve high and uniform radial resolution.

• Proceedings of the Korean Society of Applied Pharmacology
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• 1997.04a
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• pp.107-107
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• 1997

Cytochrome P450 enzymes have been intensively investigated in hepatic tissues and several mammalian cell lines. Compared to most studies about cytochrome P450 isozymes in liver in vivo and hepatic, cell lines in vitro, the study of cytochrome P450IA1 in human breast cancer cells could be very important to understand the mechanism of the regulation of CYPIA1 gene expression and cell growth. MCF-7 human breast cancer cells are well characterized to study estrogen and antiestrogen action due to the fact that they contain high level of estrogen receptor and have biological markers characterized. And also MCF-7 cells express high level of arylhydrocarbon hydroxylase activity and human cytochrome P450IA1 cDNA was cloned from MCF-7 cells. Ah receptor was characterized in many breast cancer cell lines and polycyclic aromatic hydrocarbon such as 3-MC induced the expression of CYPIA1 gene and cytochrome P450- dependent monooxygenase activity. We undertook a study to examine the effect of estrogens and other chemicals on the regulation of human CYPIA1 gene expression in MCF-7 cells via RTPCR analysis, that might help us to understand the mechanism of the regulation of CYPIA1 gene expression and MCF-7 cell growth. Expression vector containing the functional 5'-regulatory region of human CYPIA1 fused to the CAT reporter gene was transfected into estrogen receptor positive MCF-T cells or estrogen receptor negative MDA-MB-231 cells. After these cells were treated with various chemicals, RTPCR was carried out to measure both CYPIA1 mRNA and CAT mRNA levels. 1nM 3-MC increased in both P450 and CAT mRNA levels over those of control by two folds in MCF-7 cells but does not in MDA-MB-231 cells. Estrogen or tamoxifen or retinoic acid or chrysin decreased in both P450 and CAT mRNA levels that were induced by 3-MC in MCF-7 when each chemical was administered with 3-MC concomitantly. These results suggested that the level of CYPIA1 gene expression is modulated with estrogen-related molecules and make it possible to speculate that ER is related to CYPIA1 gene expression and cell growth in breast cancer cells. [Supported by grants from the Korean Ministry of Education ]

• The Korea Journal of Herbology
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• v.24 no.4
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• pp.215-224
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• 2009

Objectives : Atopic dermatitis is a chronic inflammatory disease characterized by typically distributed eczematous skin lesion with pruritus, lichenification and dry skin. In this study, we performed to assess the therapeutic effects of co-treatment of Chenilyeomgamibang (CGB) and Chenggihaedok-san (CHS, C&C) on the TNCB(trinitrochlorobenzene)-induced atopic dermatitis in NC/Nga mice, characterized by the onset of atopic dermatitis along with an increase the number of inflammatory cells and dysregulation of Th2 cytokines. Methods : Defined amount of CGB was sprayed on mice skin and CHS was simultaneously orally administrated to TNCB treated NC/Nga mice for 5 weeks. The immune cell types were caracterized by flow cytometry using each specific antibody. The amount of Th2 cytokines in serum and splenocytes culture supernatant were measured by ELISA. Results : Administration of C&C significantly reduced clinical dermatitis severity including pruritus, edema, eczematous and erythema. Histological findings indicated that the thickening of epidermis/dermis and dermal infiltration of inflammatory cells were dramatically reduced. Flow cytometry analysis showed that infiltrated immune cell numbers of CCR3+, B220+/IgE+, Gr-1+/CD11b+, and CD117+ were significantly reduced in C&C-treated dorsal skin lesion. Furthermore, T cell composition rate in PBMC was also dramatically decreased by the treatment. C&C greatly down-regulated production of Th2 cytokines including IL-4, IL-5, IL-13 in the serum. The down- regulatory effects of C&C on these Th2 cytokines production were also detected in CD3/ CD28 activated splenocytes. Conclusions : These results indicated that C&C is a plausible therapeutic agent for treatment of atopic dermatitis through regulating the Th2 skewed immune system.

• Journal of the Korean Geotechnical Society
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• v.22 no.6
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• pp.27-40
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• 2006

Damage of cut-and-cover tunnel lining can be attributed to physical and mechanical factors. Physical factors include material property, reinforcement corrosion, etc. while mechanical factors include underground water pressure, vehicle loads, etc. This study is limited to the modeling of rigid circular cut and cover tunnel constructed at a depth of $1.0{\sim}1.5D$ in loose sandy ground and subjected to a vibration frequency of 100 Hz. In this study, only damages due to mechanical factors in the form of additional loads were considered. Among the different types of additional, excessive earth pressure acting on the cut-and-cover tunnel lining is considered as one of the major factors that induce deformation and damage of tunnels after the construction is completed. Excessive earth pressure may be attributed to insufficient compaction, consolidation due to self-weight of backfill soil, precipitation and vibration caused by traffic. Laboratory tunnel model tests were performed in order to determine the earth pressure acting on the tunnel lining and to investigate the applicability of existing earth pressure formulas. Based on the difference in the monitored and computed earth pressure, a factor of safety was recommended. Soil deformation mechanism around the tunnel was also presented using the picture analysis method.

• Journal of The Korean Society of Grassland and Forage Science
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• v.6 no.3
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• pp.169-173
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• 1986

This experiment was conducted to provide comparative data on the newly imported thirteen sorghum-sudangrass hybrids(Sorghum bicolor(L.) Moench), TE Goldmaker sorghum hybrid (S. Bicolor (L.) Moench), NC+ 88SS sudangrass hybrid (S, bicolor (L.) Moench), Supermill pearlmillet (Pennisetum americanum (L.) Leeke) and teosinte (Euchlaena mexicana Schrad,) in comparison with the check variety Pioneer 988 sorghum-sudangrass hybrid for dry matter yield, protein content, in vitro dry matter digestibility(IVDMD), and other desirable agronomic characteristics, SX-17 and NC+855 gave significantly higher dry matter yield than the check variety. There was no significant difference in total dry matter yield of the remaining 14 varieties with the exception of teosinte and TE Goldmaker which had the lowest yield. The most varieties were moderately resistant to leaf diseases although differences exist among varieties. No diseases were found in pearlmillet and teosinte, but NC+ 88SS was very susceptible to leaf blight. The mean percent crude protein of sorghum-sudangrass hybrids was lower than that of pearlmillet and sorghum hybrid, but was higher than that of sudangrass hybrid, The IVDMD of G-83F, TE Goldmaker, teosinte and HW 5111 was higher than that of the check variety. There was no consistent relationship between the percent of crude protein and IVDMD of the summer annual grasses.