• Journal of Food Hygiene and Safety
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• v.25 no.4
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• pp.346-353
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• 2010

This study was conducted to determine cleaning effects of environmental-friendly washing detergent on milking installation in dairy farms. Milking machine, cooling machine and milking liner were washed by alkaline detergent and acid detergent for first three weeks and alkaline detergent and environmental~friendly detergent for next three weeks and the results get through microorganism and physico-chemistry analysis at the five district dairy farms different from environment. E-coli, coliform, fecal coliform, staphylococcus aureus, fecal streptococcus, prsudomonas aeruginosa and yersinia were not found in all of the dairy farms, and total colony counts were no difference compared with chemical detergent. The water for washing exceeded the determining acceptable level of nitrate nitrogen in the Anseong, Onyang and Cheonan and the remaining substance of washed water was also high levels. The cause of the result was that the level of nitrate nitrogen of water is basically high. Therefore, this study indicated that washing effect is no differences between environmental-friendly detergent and chemical detergent by washing for milking machine and cooling machine. Above all, producing high quality milk is to manage the water quality as well as using detergent.

• Journal of Food Hygiene and Safety
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• v.33 no.3
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• pp.193-199
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• 2018

In this study, PAT protein of genetically modified maize was prepared from the recombinant E. coli strain BL21 (DE3), and mice were immunized with the recombinant PAT protein. After cell fusion and cloning, two hybridoma cells (PATmAb-7 and PATmAb-12) were chosen since the monoclonal antibodies (Mabs) produced by them were confirmed to be specific to PAT protein in the indirect enzyme-linked immunsorbent assay (ELISA) and western blot tests. There were no cross-reactions of either Mabs to other GM proteins or to the extracts of non-GM maize. The ELISA based on the PATmAb-7 can sensitively detect 0.3 ng/g PAT protein in corn. These results indicate that the developed Mabs can be used as bio-receptors in the development of immunosensors and biosensors for the rapid and simple detection of GM corn adulterated in foods.

• Journal of Food Hygiene and Safety
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• v.33 no.5
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• pp.369-374
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• 2018

In this study, Lactobacillus brevis SM61 from traditional Kimchi was used for fermentation of aqueous extract of P. brevitarsis larva and mountain ginseng. As measured by MTT assay, aqueous extract of P. brevitarsis larva and fermented mixture of aqueous extract and mountain ginseng did not show specific cellular toxicity in RAW264.7 cells until a concentration of $5-1000{\mu}g/mL$. The polyphenol contents was highest in the fermented mixture of aqueous extract and mountain ginseng. DPPH radical scavenging activity was stronger in the fermented mixture of aqueous extract and mountain ginseng than the aqueous extract. Also, antibacterial activity was tested against E. coli, L. monocytogenes and S. aureus. The fermented mixture of aqueous extract and mountain ginseng showed antibacterial activity against the tested bacteria. Therefore, L. brevis SM61 as a starter might be used to improve functionality of P. brevitarsis larva.

• The Journal of the Korean Society for Microbiology
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• v.19 no.1
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• pp.11-24
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• 1984

Shigella remains to be an important enteric pathogen in this country for the present. Moreover, most of the isolates have become multiple resistant to various antibiotics which used to be drugs of choice for shigellosis. This study was made as an attempt to assess the present stage of antibiotic resistance and the incidence and transferability of R factors of Shigella. A total of one hundred and seventeen strains of Shigella isolated from patients in Seoul and provincial area between 1982 and 1983 were tested for their resistant to antimicrobial agents and transmission of R-plasmid. Antibiotic susceptibilities were determined by an agar dilution method. Muller hinton agar were used for the assay of drug resistance and tryptic soy broth were used for propagating medium for conjugation. Shigella isolated found to be one or more antibiotics were considered potential donor of R-plasmid. The following results were obtained. 1. Among 117 strains of Shigella isolated, 111 strains(94.9%) were found to be resistant to one or more drugs tested and 97.3% of these resistant strains were multiply resistant, indicating the multiply resistant strains were more than the single resistant strains. Only six strains were susceptible to all drugs tested. 2. Among 117 strains of Shigella isolated, 107 strains(91.5%) were resistant to Tetracyclin(Tc), 106 strains(90.6%) to Chloramphenicol(Cp) and Streptomycin(Sm), 97 strains(82.9%) to Ampicillin(Ap), 68 strains(58.1%) to Cephaloridine(Cr), 10 strains(8.5%) to Nalidixic acid(Na), 5 strains(4.3%) to Kanamycin(Km) and 2 strains(1.7%) to Rifampicin. No strain was resisfant to Amikacin(Ak) and Gentamicin(Gm). 3. All drug-resistant Shigella strains, except three, were multiply resistant to two or more drugs. Fifty eight strains were resistant to five drugs, followed by 26 strains resistant to dour drugs, 12 strains resistant to three drugs and 11 strains resistant to six drugs. 4. The 73% of multiply drug-resistant Shigella transferred their resistance to E. coli by conjugation and the resistance was considered to be mediated by R-plasmid. Resistance to Nalidixic acid and Rifampicin were not transferred by conjugation to recipient. As for the transferability of resistance to each seperate drug, Ap resistance was transferred with 73.2% frequence and Cm and Tc resistance were transferred with approximately 50-60% frequence whereas Sm and Cr resistance were transferred in 19.1-21.4% The other four drugs resistant failed to transfer their resistance to recipient. 5. As for the incidence and transferability of resistance to each seperate drug, the strains resistant to Tc and Cm were encountered most frequently with the rate of 91-92%, whereas transfer of Tc and Cm were low, 51-52%. The incidence of Sm resistance was very high(90.6%) but transferability of drugs resistance was much lower(25.4%). Though the incidence of Km reristance was much lower(4.3%) transferability of Km resistance was considerably higher(60%). 6. The greater the multiplicity of resistance, the greater was the likelihood that part of all of the resistance markers would be transferable.

• Korean Journal of Ecology and Environment
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• v.43 no.4
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• pp.492-502
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• 2010

The study objects were to analyze long-term and seasonal variations of nutrients (N, P), suspended solids, N:P ratios, algal chlorophyll, and trophic state along with general water quality parameters in four sampling sites including two intake tower sites supplying drinking water in Daechung Reservoir. For the analysis, we used water quality long-term data sampled during 1998~2007 by the Ministry of Environment, Korea. Interannual and seasonal trends in inflow and discharge near the intake tower facilities over the ten years were directly influenced by rainfall pattern. The distinct difference between wet year (2003) and dry year (2001) produced marked differences in water temperature, pH, dissolved oxygen, organic matter contents, nutrients, and these variables influenced algal biomass and trophic state. Values of TP varied depending on the year and locations sampled, but monthly mean TP always peaked during July~August when river inflow and precipitation were maxima. In contrast, TN varied little compared to TP, indicating lower influence by seasonal flow compared to phosphorus. The number of E. coli were highest in Site 2 (Chudong intake tower) and varied largely, whereas at other sites, the numbers were low and low variations. Contents of chlorophyll-${\alpha}$ (CHL), as an estimation of primary productivity, varied largely depending on the year and season. The maximum of CHL occurred at Muneu intake tower (S4) during 2006 when the precipitation and inflow were lowest. In contrast, another CHL peak was observed in Site 2 (Chudong intake tower) in 2006 when one of the largest typoons (Ewinia) occurred and river runoff were maximum. So the CHL maxima were associated with both wet year (high flow, high nutrient supply) and dry year (low flow, nutrient supply by littoral zone). Such conditions influenced trophic states, based on Trophic State Index of nutrients and CHL. Based on all analyses, we can provide some clues for management and protection strategies of two intake tower sites.

• Korean Journal of Food Science and Technology
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• v.31 no.2
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• pp.433-440
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• 1999

To develop Korean-style hot sauce using red pepper powder and/or Kochujang, its physiochemical and microbiological characteristics were investigated during storage at 20 and $30^{\circ}C$ for 56 days. The capsanthin contents and the value of surface color decreased during storage, but pH and titratable acidity were not changed. The sensory quality showed a decreased tendency during storage. Among the microorganisms, viable cell count decreased, but yeast, lactic acid bacteria and E. coli were not detected during storage. From the correlationship between sensory color and capsanthin content, the shelf-lives of hot sauce were predicted to be 221.7 days at $20^{\circ}C$, and 85.3 days at $30^{\circ}C$. While they were 230.0 days at $20^{\circ}C$ and 70.0 days at $30^{\circ}C$ in case of using Kochujang, hot sauce using both materials showed shelf-lives of 204.0 days at $20^{\circ}C$, 67.3 days at $30^{\circ}C$, respectively.

• KSBB Journal
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• v.20 no.2 s.91
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• pp.112-115
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• 2005

E. coli in combination with bacteriophage $\lambda$ was used to overcome the intrinsic plasmid instability that is frequently found in recombinant fermentation especially in long-term operation. In order to enhance the stability and productivity, the bacteriophage ${\lambda}NM1070$ was used in this study. It is a $\lambda$ mutant, which is deficient in the synthesis of protein related to DNA packaging and cell lysis. The ${\lambda}NM1070$ is also a temperature-sensitive mutant. To optimize the production of recombinant protein in this temperature-sensitive system, the temperature effects on growth and cloned gene expression were investigated for stable and efficient recombinant gene expression. The induction to the lytic state was not complete at $36^{\circ}C$ while the temperature above $40^{\circ}C$ induced the lytic state completely. However, the productivity was decreased at $42^{\circ}C$ by temperature inhibition. The L-free cell concentration increased with the increase of temperature until $40^{\circ}C$. In conclusion, ${\lambda}NM1070$ has the optimal temperature at $38^{\circ}C$ for stability and at $40^{\circ}C$ for expression.

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.6
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• pp.691-695
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• 2008

The study was carried out to evaluate the antimutagenic effects in methanol extracts of Korean soybean paste (doenjang) added with various kinds of water (germanium water, painted maple sap) or salt (sun-dried salt, roasted salt, one time bamboo roasted salt, nine times bamboo roasted salt). Methanol extracts of germanium water doenjang (Ge-D) and painted maple sap (Acer mono Max) doenjang (PM-D) exhibited significant inhibitory activity ($56{\sim}62%$) against aflatoxin $B_1$ ($AFB_1$) by adding of 1 mg/plate in Ames test. Also, methanol extracts of Ge-D and PM-D showed stronger antimutagenic activity toward N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in SOS chromotest than traditional doenjang (TD). Methanol extracts of doenjang made with four kinds of salt revealed antimutagenic activity toward MNNG; especially, doenjang extracts using one-time bamboo roasted salt (B1-D) showed 94% inhibition at the concentration of 5 mg/plate. Methanol extracts of B1-D also had the strongest inhibitory effect against MNNG of doenjang made with different salts in SOS chromotest. As the results indicate, the various kinds of water and salt have had separate effects on the antimutagenic activity of doenjang; therefore, further research on various physiological functions of water or salt added traditional doenjang is needed.

• Proceedings of the Korean Society of Applied Entomolohy Conference
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• 2006.09a
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• pp.45-60
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• 2006

최근 국제적으로 생물자원의 중요성이 재인식됨에 따라 이들에 대한 체계적이고 안정적인 정보의 구축 및 활용이 국가경쟁력의 척도로 간주할 정도로 중요성이 커지고 있는 실정이다. 또한, 우리나라는 국토의 64%가 산림으로 되어 있어서 다양한 생물종이 서식하는데 매우 적합한 조건을 갖추고 있다. 이에 따라 산림청과 국립수목원은 국가식물자원정보시스템에 이어 곤충정보에 대한 정보화 사업을 2001년부터 추진해오고 있으며 이 결과로 현재 33만여 점에 곤충표본정보가 구축되기에 이르렀다. 현재까지 국내 유수 곤충표본보유기관 22개 기관이 본 사업에 참여하여 다양하고 내실 있는 정보구축이 이루어져 왔으며 이를 통해 명실 공히 국가적인 곤충자원정보망을 갖추게 되었다. 곤충DB의 국가적인 사업이 추진된 지 6년차에 이르는 2006년도에는 정보통신부의 지원을 받아 14개 표본보유기관이 참여하여 곤충표본DB구축을 중점 추진할 계획이다. 2006년도의 목표량은 곤충표본정보 17,000점으로 기존구축자료 이외에 추가가 필요한 표본정보위주로 구축될 예정이다. 또한, 이미 구축된 곤충표본자원의 활용도와 가치를 높이기 위해서는 더욱 다양하고 많은 자료의 표본을 추가하여 DB화를 추진함은 물론 이들 각각의 정보들을 식물정보와 연계하여 분석 가능토록 하고 GIS시스템을 도입하여 명실공히 국가적인 곤충자원정보의 종합관리가 될 수 있도록 추진하고 있다. 본 사업이 충실히 수행될 경우 국가 주요 생물자원 중 하나인 곤충정보의 DB확대 구축을 통한 전체적인 현황파악 및 체계적인 관리가 가능해 질 것이며, 이와 관련하여 정보화적인 측면, 경제적 측면, 사화 문화적 측면에서 다양한 효과가 기대되며 앞으로도 이에 대한 내실 있는 운영을 위해서는 정부차원의 종합적인 지원 및 관리가 요구되는 시점으로 판단된다.pm3.42$, 저층수 $23.43\pm3.38$이었으며, 전반적으로 해역별 수질기준 I등급 내지는 II등급을 유지하고 있었고, 공간적으로는 외해측으로 갈수록 외해수와 혼합 확산되어 양호한 수질을 나타내었다. 장기적인 변동특성은 세그룹으로 구분되어진다.기 실험결과 용출용매로 증류수와 해수를 이용했을 때, 제강 슬래그에서 용출되는 납, 구리, 카드뮴, 수은의 용출 경향의 차이를 확인할 수 있었고 이에 따라서, 납, 구리, 카드뮴의 용출 유해성은 낮기 때문에 해양구조물로의 제강슬래그 유효이용은 적합할 것으로 판단되었다.im80%$로 계산되었다. 열형광선량계로 측정된 방사선량은 각각 1.8, 1.2, 0.8, 1.2, 0.8 (70 cm 거리) cGy로 측정되었으며, 환자의 복부 표면에서의 서베이메터를 이용한 측정량은 10.9 mR/h였다. 차폐구조물의 사용 시 전체 치료 동안에 태아선량은 약 1 cGy 정도로 평가되었다. 결론 : AAPM Report No.50의 자료에 따르면, 임산부의 방사선 치료 시 태아의 방사선 피폭선량은 5 cGy 이하일 경우에 방사선 피폭에 따른 태아의 위험이 거의 없는 것으로 제시되고 있다. 본원에서 차폐 구조물을 설치하였을 경우에 측정된 태아선량은 약 1 cGy로 측정되었고, 고안된 차폐구조물은 태아에 도달하는 방사선량을 감소시키기에 적합한 설계임이 입증되었다. 아니라 일반종합병원에서도 CTX-M형 ESBL 생성 E. coli와 K. pneumoniae가 존재하며 확산 중임을 시사한다. 앞으로 CTX-M형 ESBL의 만연과 변종 CTX-M형 ESBL의 출연을 감시하기 위한 정기적인 연구와 조사가 필요한 것으로 생각한다., A2-1, B1-1, B2-1의 경우, 강우 일수 감소 이전과 연 유출량 변화는 거의

• Journal of Plant Biology
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• v.38 no.1
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• pp.87-93
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• 1995

For understanding physiological nature of phototaxis in Synechocystis sp. PCC 6803 PTX(S. 6803 PTX), we examined the effects of some metabolic inhibitors and cation ionophore on the phototactic movement. In the presence of DCMU, which blocks the photosynthetic electron transport just after photosystem II acceptor, there was no inhibitory effect on the phototaxis up to $100\;\mu\textrm{M}$. Instead, the respiratory electron chain inhibitor such as sodium azide dramatically impaired the phototaxis in S. 6803 PTX. These observations indicate that the phototaxis is linked not to photo-phosphorylation, but to respiratory phosphorylation. When the cells were treated with un couplers such as CCCP or DNP, which dissipate the electrochemical gradient of proton($\Delta\mu_{H}+$) across the cytoplasmic membrane, these chemicals did not affect phototaxis. In contrast, when cells were treated with DCCD or NBD which deprive cells of A TP but leave $\Delta\mu_{H}+$ intact across the membrane, the phototactic movement was severly reduced. These results imply that ATP production, not proton motive force, is involved in the phototactic movement in this organism as a driving motive force. The application of specific calcium ionophore A23187 strongly impaired positive phototaxis. Calcium fluxes should be engaged in the sensory trans-duction of phototactic orientation. Finally, when ethionine was supplimented to culture media, the photomovement of this organism was inhibited. This implies that methylation/demethylation mechanism controls the process of phototaxis in S. 6803 PTX like chemotaxis in E. coli and Salmonella typhimurium.murium.