• Clinical and Experimental Reproductive Medicine
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• v.47 no.2
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• pp.94-100
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• 2020

The inverse correlation between maternal age and pregnancy rate represents a major challenge for reproductive endocrinology. The high embryo ploidy error rate in failed in vitro fertilization (IVF) cycles reflects genetic misfires accumulated by older oocytes over time. Despite the application of different follicular recruitment protocols during IVF, gonadotropin modifications are generally futile in addressing such damage. Even when additional oocytes are retrieved, quality is frequently poor. Older oocytes with serious cytoplasmic and/or chromosomal errors are often harvested from poorly perfused follicles, and ovarian vascularity and follicular oxygenation impact embryonic chromosomal competency. Because stimulation regimens exert their effects briefly and immediately before ovulation, gonadotropins alone are an ineffective antidote to long-term hypoxic pathology. In contrast, the tissue repair properties (and particularly the angiogenic effects) of platelet-rich plasma (PRP) are well known, with applications in other clinical contexts. Injection of conventional PRP and/or its components (e.g., isolated platelet-derived growth factors as a cell-free substrate) into ovarian tissue prior to IVF has been reported to improve reproductive outcomes. Any derivative neovascularity may modulate oocyte competence by increasing cellular oxygenation and/or lowering concentrations of intraovarian reactive oxygen species. We propose a mechanism to support intrastromal angiogenesis, improved follicular perfusion, and, crucially, embryo ploidy rescue. This last effect may be explained by mRNA upregulation coordinated by PRP-associated molecular signaling, as in other tissue systems. Additionally, we outline an intraovarian injection technique for platelet-derived growth factors and present this method to help minimize reliance on donor oocytes and conventional hormone replacement therapy.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.20 no.1 s.32
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• pp.177-194
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• 2007

Fructus of Rubus coreanus $M_{IQ.}$. (FRCM) has been used for stuttering urination, prostate gland disease, and impotence in Korean traditional medicine. Water extract of FRCM (WFRCM) treatment significantly attenuated airway hyperreactivity (AHR). Airway recruitment of leukocytes and eosinophils was also markedly reduced by WFRCM administration, suggesting that WFRCM can alleviate the airway inflammation. However, the level of cytokine (IL-4, IL-5, and IL-13) in bronchoalveolar lavage fluid (BALF) and lung was not different compared with positive control. WFRCM reduced the number of draining lymph node cells during OVA-induced allergic asthma. I further examined transcription level of cytokine in lung. WFRCM treatment reduced IL-4 and IL-13 mRNA in lung and inhibited IgE and IgG1 but not IgG2a. My data suggest that WFRCM attenuates OVA-induced allergic asthma through inhibition of Th2 cell response.

• IMMUNE NETWORK
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• v.23 no.3
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• pp.25.1-25.15
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• 2023

Mucosal environments harbour lymphocytes, which express several adhesion molecules, including intestinal homing receptors and integrin αE/β7 (CD103). CD103 binds E-cadherin, an integrin receptor expressed in intestinal endothelial cells. Its expression not only enables homing or retention of T lymphocytes at these sites but is also associated with increased T lymphocyte activation. However, it is not yet clear how CD103 expression is related to the clinical staging of breast cancer, which is determined by factors such as the size of the tumor (T), the involvement of nearby lymph nodes (N), and presence of metastasis (M). We examined the prognostic significance of CD103 by FACS in 53 breast cancer patients and 46 healthy controls enrolled, and investigated its expression, which contributes to lymphocyte recruitment in tumor tissue. Patients with breast cancer showed increased frequencies of CD103⁺, CD4⁺CD103⁺, and CD8⁺CD103⁺ cells compared to controls. CD103 was expressed at a high level on the surfaces of tumor-infiltrating lymphocytes in patients with breast cancer. Its expression in peripheral blood was not correlated with clinical TNM stage. To determine the localisation of CD103⁺ cells in breast tissue, tissue sections of breast tumors were stained for CD103. In tissue sections of breast tumors stained for CD103, its expression in T lymphocytes was higher compared to normal breast tissue. In addition, CD103⁺ cells expressed higher levels of receptors for inflammatory chemokines, compared to CD103^- cells. CD103⁺ cells in peripheral blood and tumor tissue might be an important source of tumor-infiltrating lymphocyte trafficking, homing, and retention in cancer patients.

• Journal of Life Science
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• v.26 no.1
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• pp.140-145
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• 2016

Genes in multicellular organisms are transcribed in development, differentiation, or tissue-specific manners. The transcription of genes is activated by enhancers, which are transcription regulatory elements located at long distances from the genes. Recent studies have reported that noncoding RNAs are transcribed from active enhancers by RNA polymerase II (RNA Pol II); these are called enhancer RNAs (eRNAs). eRNAs are transcribed bi-directionally from the enhancer core, and are capped on the 5’ end but not spliced or polyadenylated on the 3’ end. The transcription of eRNAs requires the binding of transcription activators on the enhancer and associates positively with the transcription of the target gene. The transcriptional inhibition of eRNAs or the removal of eRNA transcripts results in the transcriptional repression of the coding gene. The transcriptional procedure of eRNAs causes enhancer- specific histone modifications, such as histone H3K4me1/2. eRNA transcripts directly interact with Mediator and Rad21, a cohesin subunit, generating a chromatin loop structure between the enhancer and the promoter of the target gene. The recruitment of RNA Pol II into the promoter and its elongation through the coding region are facilitated by eRNAs. Here, we will review the features of eRNAs, and discuss the mechanism of eRNA transcription and the roles of eRNAs in the transcriptional activation of target genes.

• The Journal of the Convergence on Culture Technology
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• v.9 no.4
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• pp.283-290
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• 2023

This study investigates the influential factors on online word-of-mouth (eWOM) intentions towards educational tourist destinations, primarily focusing on Korean universities and Chinese tourists. It reveals a positive correlation between eWOM intentions and the desire to enroll in these institutions. The significance of eWOM for attracting Chinese students elucidates the importance of establishing a strong brand image and ensuring high levels of tourist satisfaction. Moreover, social media users who travel for education purposes were found to emphasize self-image consistency and conspicuous self-expression. This research underscores the utility of leveraging social media to enhance the perception of educational tourist attractions, promoting these destinations, and stimulating eWOM, thereby enriching the understanding of the strategic use of social media in international student recruitment.

• Korean Journal of Ichthyology
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• v.20 no.3
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• pp.190-197
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• 2008

Spatio-temporal distribution of eggs and larvae of Japanese anchovy Engraulis japonica in the southern sea of Korea was determined using data obtained from icthyoplanktonic surveys and oceanographic observations between Goheung Peninsula and Goeje Island from May to August 2007. Commercial anchovy catch and size composition from four local fishery cooperative associations were also analyzed in relation to the geographic distribution of eggs and larvae. The abundance of anchovy eggs increased from May to July and was lowest in August. Eggs were mainly distributed between Namhae Island and Goeje Island, where water temperatures were $15{\sim}24^{\circ}C$ and salinities were 32~35‰. In 2007 anchovy landings between Namhae Island and Goeje Island were 11,409 tons at Tongyeong association, 4,137 tons at Masan association, and 2,487 tons at Sacheon association. However, landings between Goheung and Yeosu Peninsula were only 4,411 tons (at Yeosu association). The catch by anchovy tow net was high in the area where eggs were abundantly distributed. This indicates that the distribution of egg density was directly correlated with recruitment of E. japonica. All growth stages were abundant in the study area, indicating that this area is a major spawning and growing ground of E. japonica in Korean waters.

• Journal of KIISE
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• v.43 no.3
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• pp.336-345
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• 2016

Since no course is offered in the area of professional ICT(Information & Communication Technology) in the high school curriculum, high school students do not have the opportunity to learn about the ICT engineering area and the possible career paths in the field. Due to this problem, high school students are not motivated to choose ICT majors at university level, and in turn, the ICT departments are struggling to recruit qualified students. In this paper, we present an R&E (research and education) model to mitigate this problem. We also present a case study on the program following the model offered by "H" university in collaboration with a local high school. Through the program we provided high school students with design and development experiences to solve engineering problems related to the ICT area and tried to attract their attention to ICT majors. The participants of the R&E program were able to experience the systematic engineering design process, ICT tools, teamwork, and communication skills through problem solving procedures. Based on three years of observation and the survey, it was found that more than 76% of students were motivated highly by ICT and engineering majors via the program. The main contribution of the paper is that we have proposed and proved the R&E program model and applied the ICT R&E model to a program to attract qualified students to ICT majors.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.31 no.5
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• pp.654-664
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• 1998

Seasonal variation in species composition and abundance of pelagic fish in Chonsu Bay were determined by analyzing monthly samples collected using a set net from March, 1992 to January, 1993. The data in this study were compared with those of 1981$\~$82. Of 63 species identified, Engraulis japonicus, Ammodytes penonatus, Enedrias fangi and Sardinella zunasi predominated in abundance. Especially E.japonicus occupied $87\%$ of total number of individuals. In spling, fish abundance increased by recruitment of lariat E. fanti as well as adults of E. japonicus, A. personatus and S. zunasi. These adults moved towards the open sea after spawning in early summer. A large number of juveniles was then collected from August until December. The seasonal change in species composition from the present study showed a similar trend to that occurred in 1981$\~$82. However, larger number of pelagic fish was caught in 1992$\~$93 than in 1981$\~$82. This result seems to be related to the higher water temperature (>$2^{\circ}C$) than the average mean temperature in spring and autumn, implying that higher temperature provides favorable conditions for spawning and growth of the fish.

• IMMUNE NETWORK
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• v.15 no.4
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• pp.206-211
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• 2015

Pulmonary edema is a major cause of mortality due to acute lung injury (ALI). The involvement of protein kinase C-${\delta}$ (PKC-${\delta}$) in ALI has been a controversial topic. Here we investigated PKC-${\delta}$ function in ALI using PKC-${\delta}$ knockout (KO) mice and PKC inhibitors. Our results indicated that although the ability to produce proinflammatory mediators in response to LPS injury in PKC-${\delta}$ KO mice was similar to that of control mice, they showed enhanced recruitment of neutrophils to the lung and more severe pulmonary edema. PKC-${\delta}$ inhibition promoted barrier dysfunction in an endothelial cell layer in vitro, and administration of a PKC-${\delta}$-specific inhibitor significantly increased steady state vascular permeability. A neutrophil transmigration assay indicated that the PKC-${\delta}$ inhibition increased neutrophil transmigration through an endothelial monolayer. This suggests that PKC-${\delta}$ inhibition induces structural changes in endothelial cells, allowing extravasation of proteins and neutrophils.

• Korean Journal of Oriental Medicine
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• v.13 no.2 s.20
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• pp.127-133
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• 2007

Objectives : This study were aimed to estimate the needs for clinical research educational program development focused on oriental medicine. Methods : Department Medical Research of Korea Institute of Oriental Medicine surveyed 204 certified subjects in total by using web-based questionnaires through e-mail from Jan. 17th to Jan. 31th, 2007. Reply from 62 on-line correspondents were collected and statistically analyzed. Results : The number of clinical trial involving oriental medicine continues to increase. According to the survey, many Issues were raised as problems such as difficulty of recruitment, lack of fund and lack of stepped program. Emphasized issues were clinical research methodologies, development of protocols and case report form (CRF), and Regulations including Institutional Review Boards (IRBs) in these three consecutive education training program. Conclusion : The results of this study may contribute to the development of an educational program for oriental medicine, a program that should be taken into consideration for developing practical items, such as, problem-based learnings which reflects participants' actual needs in their works. Also this report be used for future strategy plans and execution of training program for oriental medical clinical trial.