• 제목/요약/키워드: E-O Sampling

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도체표면의 분변오염과 Verotoxin 생성 Escherichia coli O157:H7 분리에 관한 연구 (Isolation of Verocytotoxin Producing Escherichia coli O157:H7 Due to Fcal Contamination on Carcass Surfaces)

  • 홍종해;고주언
    • 한국식품위생안전성학회지
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    • 제12권1호
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    • pp.78-82
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    • 1997
  • Surface swab samples from beef (188), pork (240) and chicken (95) carcasses were collected from slaughterhouse in Kangwon and Kyunggi areas from March through July 1996. The samples were examined on the level of E. coli biotype I relevant to fecal contamination due to unsanitary processing control and the existence of verocytotoxin-producing E. coli (VTEC). E. coli biotype I were confirmed from 38.8% of beef, 40.0% of pork, and 69.5% of chicken carcasses. Little variation was noted among three sampling points; rump, flank and neck of beef, ham, belly and jowls of pork. coli O157:H7 was only confirmed from 2 of 188 beef carcasses. E. coli biotype I. All the isolated E. coli O157 showed positive for vero cell cytotoxicity test. Isolation rate of E. coli O157 in summer was higher than in spring. In case of pork and chicken carcasses, E. coli O157 was isolated in summer only.

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THE AVERAGING VALUE OF A SAMPLING OF THE RIEMANN ZETA FUNCTION ON THE CRITICAL LINE USING POISSON DISTRIBUTION

  • Jo, Sihun
    • East Asian mathematical journal
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    • 제34권3호
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    • pp.287-293
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    • 2018
  • We investigate the averaging value of a random sampling ${\zeta}(1/2+iX_t)$ of the Riemann zeta function on the critical line. Our result is that if $X_t$ is an increasing random sampling with Poisson distribution, then $${\mathbb{E}}{\zeta}(1/2+iX_t)=O({\sqrt{\;log\;t}}$$, for all sufficiently large t in ${\mathbb{R}}$.

Intersymbol interference due to sampling-time jitter and its approximations in a raised cosing filtered system

  • 박영미;목진담;나상신
    • 한국통신학회논문지
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    • 제21권11호
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    • pp.2942-2953
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    • 1996
  • This paper studies the effect of intersymbol interference due to sampling-time jitter on the worst-case bit error probability in a digital modultation over an additive white Gaussian noise channel, with the squared-root raised-cosine filters in the transmitter and the receiver. It derives approximation formulas using the Taylor series approximations. the principal results of this paper is the relationship between the worst-casse bit error probability, the degree of jitter, the roll factor of the raised cosine filter, and other quantities. Numerical results show, as expected, that the intersymbol interference decreases as the roll-off factor increases and the jitter decreases. They also show that the approximation formulas are accurate for smally intersymbol interference, i.e., for large roll-noise ratio $E_{b/}$ $N_{0}$.leq.7 dB and begin to lose accuracy for larger signal-to-noise ratio.o.o.

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여과식 채취방법에 의한 대기오염 총침착물의 특성 -석탄화력발전소 주변지역을 중심으로- (Characteristics of Total Atmospheric Deposition by the Filtration-Sampling Method at Coal-Fired Power Plant Area)

  • 박정호;조인철;최금찬
    • 한국대기환경학회지
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    • 제18권2호
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    • pp.161-170
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    • 2002
  • Total(=wet+dry) atmospheric depositions were collected by filtration-sampling method at 17 sampling sites of the coal-fired power plant area from September 1999 to January 2000. The soluble and insoluble fractions of deposition were also measured to investigate a suitability of simplified collection method for a long-term monitoring of total deposition. In the study, the 50% of sampled soluble fractions showed the electric conductivity (E.C.) of below 50 $\mu$S/cm and the 42% of them showed the lower pH than 5.0. The monthly mean fluxes of water soluble ionic components; S $O_4$$^{2-}$, C $l^{[-10]}$ , N $O_3$$^{[-10]}$ , N $a^{+}$, N $H_4$$^{+}$, $K^{+}$, $Mg^{2+}$, $Ca^{2+}$ were 168.4 kg/k $m^2$.month, 100.5 kg/k $m^2$.month, 88.6kg/k $m^2$.month, 31.3kg/k $m^2$.month, 25.6 kg/k $m^2$.month, 13.3 kg/k $m^2$.month, 8.7 kg/k $m^2$.month, 43.1kg/k $m^2$.month, respectively. The mean ionic concentration of all sample(n=79) was 314 $\mu$eq/ι, with contributions of 24.2% and 23.0% by [nss-C $a^{2+}$] and [nss-S $O_4$$^{2-}$]. The ratio of [N $O_3$$^{[-10]}$ ]/[nss-S $O_4$$^{2-}$] and [N $H_4$-C $a^{2+}$] were found to be 0.52 and 0.68, respectively.espectively.

양산점토의 비배수 특성에 대한 시료교란의 효과 (The Effects of Sample Disturbance on Undrained Properties of Yangsan Clay)

  • 김길수;임형덕;이우진
    • 한국지반공학회:학술대회논문집
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    • 한국지반공학회 2000년도 봄 학술발표회 논문집
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    • pp.639-646
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    • 2000
  • It is important to estimate the mechanical properties of clay since it is directly related to the design and the construction of geotechnical structures. Site exploration, which is composed of boring, sampling, in-situ, or laboratory tests, is preformed to estimate the mechanical properties. However, mechanical properties of clay measured from laboratory test may be different from in-situ properties due to disturbances occurred during sampling, transportation, storage, and trimming. In this study, the degree of disturbance according to sampling method was estimated with the test results of CK/sub o/U triaxial compression test on Yangsan clay. The soil samples were obtained by three types of sampling method, j.e., 76mm-tube sampler, 76mm-piston sampler, and block sampler. In order to evaluate the quality of samples, volumetric strain, undrained shear strength, secant Young's modulus, and pore pressure coefficient at peak measured from each sample were compared with one another. From the test results, it was observed that mechanical properties of the block and piston samples were more reliable than those of tube samples. But it was observed that the water content of piston was similar to that of tube samples at given depths while the water content of block samples was 14.3∼15.8% smaller than that of piston and tube samples. In addition to the evaluation of the quality of samples, relationship between c/sub u// σ/sub vc/'and OCR was established from the results of the CK/sub o/U triaxial compression tests, which were carried out using SHANSEP method. And also undrained shear strength was analyzed using the in-situ test data such as Cone Penetration Test(CPT), Dilatometer Test(DMT), and Field Vane Test(FVT) and was compared with that evaluated from CK/sub o/U triaxial compression test.

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물놀이형 수경시설의 수질과 항생제 내성 대장균 분포에 관한 연구 (Distribution of Antibiotic Resistant Escherichia coli and Water Quality in Waterscape Facilities)

  • 윤상훈;김선경;김난희;위환;이세행;조광운;김동수;조영관
    • 한국환경보건학회지
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    • 제40권3호
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    • pp.225-233
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    • 2014
  • Objective: This study was performed in order to investigate the distribution of antibiotic resistant E. coli and water quality in waterscape facilities. Methods: Nine waterscape facilities were selected and classified into three types: ground, wall and general fountain. The items analyzed in this study were pH, turbidity, $KMnO_4$ consumption, total colony, total coliforms, fecal coliforms and E. coli. Additionally, an antibiotic resistance test on E. coli was performed. Results: There were no sampling sites that exceeded the permitted limits of water quality, but the concentration of pollutants was high at the beginning operation in June. Moreover, concentration of turbidity, $KMnO_4$ consumption and total colony were higher in ground fountains than in wall and general fountains. Five species of antibiotic resistant E. coli were detected from three sampling sites. Conclusions: The waterscape facilities were significantly contaminated with water microorganisms, especially total colony, total coliforms and fecal coliforms. Disinfection and filtration systems to remove pollutants should be installed for safe waterscape facilities.

산업단지지역 하천 미생물에 의한 퍼클로레이트 제거 (Perchlorate Removal by River Microorganisms in Industrial Complexes)

  • 조강익;안영희
    • Korean Chemical Engineering Research
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    • 제52권1호
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    • pp.92-97
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    • 2014
  • 퍼클로레이트($ClO_4^-$)는 지표수는 물론이고 토양지하수의 신규 오염물이다. $ClO_4^-$는 요오드가 갑상선에 흡수되는 것을 방해하므로 갑상선 호르몬 생성을 저하시킨다. $ClO_4^-$는 물에서 매우 용해도가 높고 안정적이라는 특징으로 인해 $ClO_4^-$를 환원하는 세균(PRB)에 의한 생분해가 자연저감의 가장 중요한 요인으로 여겨지고 있다. 산업단지 내 하천은 점 또는 비점오염원으로부터 배출된 $ClO_4^-$에 오염될 잠재성이 있다. 그래서 본 연구에서는 구미지역 산업단지 내 하천에서 물시료를 채취하여 하천미생물의 $ClO_4^-$ 분해 잠재능을 회분배양으로 조사하였다. 외부 전자공여체를 첨가하지 않고 83시간 동안 배양한 결과 모든 시료는 $ClO_4^-$ 제거효율이 0.77% 이하로 매우 낮은 것으로 나타났다. 그러나 외부 전자공여체(acetate, thiosulfate, $S^0$, 또는 $F^0$)를 첨가한 경우는 제거효율이 최고 100%로 나타났고, 첨가된 전자공여체의 종류와 시료채취지점에 따라 제거효율은 다양한 것으로 나타났다. 본 연구에서 사용한 전자공여체 중에서는 acetate를 사용했을 때 $ClO_4^-$분해효율이 가장 우수한 것으로 나타나 종속영양방식 PRB의 활성이 우세함을 알 수 있었다. 본 연구의 결과는 산업단지 내 하천 미생물에 의한 $ClO_4^-$ 자연저감에 대한 기초정보를 제공하여 원위치 생물복원처리에서 $ClO_4^-$ 생분해를 증진하기 위한 전략마련에 유용하게 사용될 것이다.

Generating global warming scenarios with probability weighted resampling and its implication in precipitation with nonparametric weather generator

  • Lee, Taesam;Park, Taewoong
    • 한국수자원학회:학술대회논문집
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    • 한국수자원학회 2015년도 학술발표회
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    • pp.226-226
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    • 2015
  • The complex climate system regarding human actions is well represented through global climate models (GCMs). The output from GCMs provides useful information about the rate and magnitude of future climate change. Especially, the temperature variable is most reliable among other GCM outputs. However, hydrological variables (e.g. precipitation) from GCM outputs for future climate change contain too high uncertainty to use in practice. Therefore, we propose a method that simulates temperature variable with increasing in a certain level (e.g. 0.5oC or 1.0oC increase) as a global warming scenario from observed data. In addition, a hydrometeorological variable can be simulated employing block-wise sampling technique associated with the temperature simulation. The proposed method was tested for assessing the future change of the seasonal precipitation in South Korea under global warming scenario. The results illustrate that the proposed method is a good alternative to levy the variation of hydrological variables under global warming condition.

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원료돈육의 오염원 추적 및 위생기준 설정에 관한 연구 (Study on Pursuit of Contamination Sources and Establishment of Sanitary Standard from Raw Pork Meat)

  • 임대석;강희곤;김용곤;김창한
    • 한국축산식품학회지
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    • 제21권2호
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    • pp.149-155
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    • 2001
  • The aims of this study were to examine contamination sources and provide the basic data in establishment of sanitary standard for raw pork meat. From \"Random sampling(I)\", initial total plate counts of post-slaughter samples for the group A, B and C were 1.5${\times}10^4$cfu/$textrm{cm}^2$, 5.5${\times}10^5$cfu/$textrm{cm}^2$ and 1.8${\times}10^4$cfu/$textrm{cm}^2$, respectively, and of post-prechilling samples for the group A, B and C were 1.0${\times}10^4$cfu/$textrm{cm}^2$, 4.6${\times}10^5$cfu/$textrm{cm}^2$ and 2.5${\times}10^4$cfu/$textrm{cm}^2$, respectively. Initial total plate counts of post-transportation samples for the group D, E and F did not increased, as did the group A, B and C. From \"Normal sampling(II)\", initial total plate counts of post-slaughter, post-prechilling, post-transportation and post-2 days preservation samples were 7.3${\times}10^4$cfu/$textrm{cm}^2$, 9.6${\times}10^4$cfu/$textrm{cm}^2$, 2.0${\times}10^5$cfu/$textrm{cm}^2$ and 2.5${\times}10^5$cfu/$textrm{cm}^2$, respectively. From \"Clean sampling(III)\", initial total plate counts of post-slaughter, post-prechilling, post-transportation and post-2 days preservation samples were decreased to 7.0${\times}10^2$cfu/$textrm{cm}^2$, 7.5${\times}10^2$cfu/$textrm{cm}^2$, 8.5${\times}10^2$cfu/$textrm{cm}^2$ and 5.5${\times}10^3$cfu/$textrm{cm}^2$, respectively, compared with "Normal sampling(II)". No E. coli O157:H7, Staphylococcus aureus and Salmonella were detected at each sampling step. Consequently, a slaughter method like "Clean sampling(III)" showed a better sanitary effect to low total plate counts of 10$^2$∼10$^3$times, compared with "Normal sampling(II)". The one of contamination sources for raw pork meat was at a slaughtering step, and "Clean sampling" method may be considered as the one of sanitary standards.

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Resistance Patterns of Frequently Applied Antimicrobials and Occurrence of Antibiotic Resistance Genes in Edwardsiella tarda Detected in Edwardsiellosis-Infected Tilapia Species of Fish Farms of Punjab in Pakistan

  • Kashif Manzoor;Fayyaz Rasool;Noor Khan;Khalid Mahmood Anjum;Shakeela Parveen
    • Journal of Microbiology and Biotechnology
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    • 제33권5호
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    • pp.668-679
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    • 2023
  • Edwardsiella tarda is one of the most significant fish pathogens, causes edwardsiellosis in a variety of freshwater fish species, and its antibiotic resistance against multiple drugs has made it a health risk worldwide. In this study, we aimed to investigate the antibiotic resistance (ABR) genes of E. tarda and establish its antibiotic susceptibility. Thus, 540 fish (299 Oreochromis niloticus, 138 O. mossambicus, and 103 O. aureus) were collected randomly from twelve fish farms in three districts of Punjab in Pakistan. E. tarda was recovered from 147 fish showing symptoms of exophthalmia, hemorrhages, skin depigmentation, ascites, and bacteria-filled nodules in enlarged liver and kidney. Antimicrobial susceptibility testing proved chloramphenicol, ciprofloxacin, and streptomycin effective, but amoxicillin, erythromycin, and flumequine ineffective in controlling edwardsiellosis. Maximum occurrence of qnrA, blaTEM, and sul3 genes of E. tarda was detected in 45% in the liver, 58%, and 42% respectively in the intestine; 46.5%, 67.2%, and 55.9% respectively in O. niloticus; 24%, 36%, and 23% respectively in summer with respect to fish organs, species, and season, respectively. Motility, H2S, indole, methyl red, and glucose tests gave positive results. Overall, E. tarda infected 27.2% of fish, which ultimately caused 7.69% mortality. The Chi-squared test of independence showed a significant difference in the occurrence of ABR genes of E. tarda with respect to sampling sites. In conclusion, the misuse of antibacterial agents has led to the emergence of ABR genes in E. tarda, which in association with high temperatures cause multiple abnormalities in infected fish and ultimately resulting in massive mortality.