• Title/Summary/Keyword: E-O Sampling

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Isolation of Verocytotoxin Producing Escherichia coli O157:H7 Due to Fcal Contamination on Carcass Surfaces (도체표면의 분변오염과 Verotoxin 생성 Escherichia coli O157:H7 분리에 관한 연구)

  • 홍종해;고주언
    • Journal of Food Hygiene and Safety
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    • v.12 no.1
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    • pp.78-82
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    • 1997
  • Surface swab samples from beef (188), pork (240) and chicken (95) carcasses were collected from slaughterhouse in Kangwon and Kyunggi areas from March through July 1996. The samples were examined on the level of E. coli biotype I relevant to fecal contamination due to unsanitary processing control and the existence of verocytotoxin-producing E. coli (VTEC). E. coli biotype I were confirmed from 38.8% of beef, 40.0% of pork, and 69.5% of chicken carcasses. Little variation was noted among three sampling points; rump, flank and neck of beef, ham, belly and jowls of pork. coli O157:H7 was only confirmed from 2 of 188 beef carcasses. E. coli biotype I. All the isolated E. coli O157 showed positive for vero cell cytotoxicity test. Isolation rate of E. coli O157 in summer was higher than in spring. In case of pork and chicken carcasses, E. coli O157 was isolated in summer only.

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THE AVERAGING VALUE OF A SAMPLING OF THE RIEMANN ZETA FUNCTION ON THE CRITICAL LINE USING POISSON DISTRIBUTION

  • Jo, Sihun
    • East Asian mathematical journal
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    • v.34 no.3
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    • pp.287-293
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    • 2018
  • We investigate the averaging value of a random sampling ${\zeta}(1/2+iX_t)$ of the Riemann zeta function on the critical line. Our result is that if $X_t$ is an increasing random sampling with Poisson distribution, then $${\mathbb{E}}{\zeta}(1/2+iX_t)=O({\sqrt{\;log\;t}}$$, for all sufficiently large t in ${\mathbb{R}}$.

Intersymbol interference due to sampling-time jitter and its approximations in a raised cosing filtered system

  • 박영미;목진담;나상신
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.21 no.11
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    • pp.2942-2953
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    • 1996
  • This paper studies the effect of intersymbol interference due to sampling-time jitter on the worst-case bit error probability in a digital modultation over an additive white Gaussian noise channel, with the squared-root raised-cosine filters in the transmitter and the receiver. It derives approximation formulas using the Taylor series approximations. the principal results of this paper is the relationship between the worst-casse bit error probability, the degree of jitter, the roll factor of the raised cosine filter, and other quantities. Numerical results show, as expected, that the intersymbol interference decreases as the roll-off factor increases and the jitter decreases. They also show that the approximation formulas are accurate for smally intersymbol interference, i.e., for large roll-noise ratio $E_{b/}$ $N_{0}$.leq.7 dB and begin to lose accuracy for larger signal-to-noise ratio.o.o.

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Characteristics of Total Atmospheric Deposition by the Filtration-Sampling Method at Coal-Fired Power Plant Area (여과식 채취방법에 의한 대기오염 총침착물의 특성 -석탄화력발전소 주변지역을 중심으로-)

  • 박정호;조인철;최금찬
    • Journal of Korean Society for Atmospheric Environment
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    • v.18 no.2
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    • pp.161-170
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    • 2002
  • Total(=wet+dry) atmospheric depositions were collected by filtration-sampling method at 17 sampling sites of the coal-fired power plant area from September 1999 to January 2000. The soluble and insoluble fractions of deposition were also measured to investigate a suitability of simplified collection method for a long-term monitoring of total deposition. In the study, the 50% of sampled soluble fractions showed the electric conductivity (E.C.) of below 50 $\mu$S/cm and the 42% of them showed the lower pH than 5.0. The monthly mean fluxes of water soluble ionic components; S $O_4$$^{2-}$, C $l^{[-10]}$ , N $O_3$$^{[-10]}$ , N $a^{+}$, N $H_4$$^{+}$, $K^{+}$, $Mg^{2+}$, $Ca^{2+}$ were 168.4 kg/k $m^2$.month, 100.5 kg/k $m^2$.month, 88.6kg/k $m^2$.month, 31.3kg/k $m^2$.month, 25.6 kg/k $m^2$.month, 13.3 kg/k $m^2$.month, 8.7 kg/k $m^2$.month, 43.1kg/k $m^2$.month, respectively. The mean ionic concentration of all sample(n=79) was 314 $\mu$eq/ι, with contributions of 24.2% and 23.0% by [nss-C $a^{2+}$] and [nss-S $O_4$$^{2-}$]. The ratio of [N $O_3$$^{[-10]}$ ]/[nss-S $O_4$$^{2-}$] and [N $H_4$-C $a^{2+}$] were found to be 0.52 and 0.68, respectively.espectively.

The Effects of Sample Disturbance on Undrained Properties of Yangsan Clay (양산점토의 비배수 특성에 대한 시료교란의 효과)

  • 김길수;임형덕;이우진
    • Proceedings of the Korean Geotechical Society Conference
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    • 2000.03b
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    • pp.639-646
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    • 2000
  • It is important to estimate the mechanical properties of clay since it is directly related to the design and the construction of geotechnical structures. Site exploration, which is composed of boring, sampling, in-situ, or laboratory tests, is preformed to estimate the mechanical properties. However, mechanical properties of clay measured from laboratory test may be different from in-situ properties due to disturbances occurred during sampling, transportation, storage, and trimming. In this study, the degree of disturbance according to sampling method was estimated with the test results of CK/sub o/U triaxial compression test on Yangsan clay. The soil samples were obtained by three types of sampling method, j.e., 76mm-tube sampler, 76mm-piston sampler, and block sampler. In order to evaluate the quality of samples, volumetric strain, undrained shear strength, secant Young's modulus, and pore pressure coefficient at peak measured from each sample were compared with one another. From the test results, it was observed that mechanical properties of the block and piston samples were more reliable than those of tube samples. But it was observed that the water content of piston was similar to that of tube samples at given depths while the water content of block samples was 14.3∼15.8% smaller than that of piston and tube samples. In addition to the evaluation of the quality of samples, relationship between c/sub u// σ/sub vc/'and OCR was established from the results of the CK/sub o/U triaxial compression tests, which were carried out using SHANSEP method. And also undrained shear strength was analyzed using the in-situ test data such as Cone Penetration Test(CPT), Dilatometer Test(DMT), and Field Vane Test(FVT) and was compared with that evaluated from CK/sub o/U triaxial compression test.

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Distribution of Antibiotic Resistant Escherichia coli and Water Quality in Waterscape Facilities (물놀이형 수경시설의 수질과 항생제 내성 대장균 분포에 관한 연구)

  • Yoon, Sang-Hoon;Kim, Seon-Gyeong;Kim, Nan-Hee;Wi, Whan;Lee, Se-Hang;Jo, Gwang-Woon;Kim, Dong-Su;Cho, Young-Gwan
    • Journal of Environmental Health Sciences
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    • v.40 no.3
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    • pp.225-233
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    • 2014
  • Objective: This study was performed in order to investigate the distribution of antibiotic resistant E. coli and water quality in waterscape facilities. Methods: Nine waterscape facilities were selected and classified into three types: ground, wall and general fountain. The items analyzed in this study were pH, turbidity, $KMnO_4$ consumption, total colony, total coliforms, fecal coliforms and E. coli. Additionally, an antibiotic resistance test on E. coli was performed. Results: There were no sampling sites that exceeded the permitted limits of water quality, but the concentration of pollutants was high at the beginning operation in June. Moreover, concentration of turbidity, $KMnO_4$ consumption and total colony were higher in ground fountains than in wall and general fountains. Five species of antibiotic resistant E. coli were detected from three sampling sites. Conclusions: The waterscape facilities were significantly contaminated with water microorganisms, especially total colony, total coliforms and fecal coliforms. Disinfection and filtration systems to remove pollutants should be installed for safe waterscape facilities.

Perchlorate Removal by River Microorganisms in Industrial Complexes (산업단지지역 하천 미생물에 의한 퍼클로레이트 제거)

  • Jo, Kang-Ick;Ahn, Yeonghee
    • Korean Chemical Engineering Research
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    • v.52 no.1
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    • pp.92-97
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    • 2014
  • Perchlorate ($ClO_4^-$) is an emerging contaminant of soil/groundwater and surface water. $ClO_4^-$ has been shown to inhibit iodide uptake into the thyroid gland and cause a reduction in thyroid hormone production. $ClO_4^-$ is highly soluble and very stable in water. Biodegradation by $ClO_4^-$-reducing bacteria (PRB) is considered the most important factor in natural attenuation of $ClO_4^-$. Rivers in an industrial complex have potential to be contaminated with $ClO_4^-$ discharged from point or non-point sources. In this study, water samples were taken from the rivers running through the Gumi industrial complexes and used for batch test to analyze $ClO_4^-$-degradation potential of river microorganisms. The results of 83-h batch culture showed that $ClO_4^-$-removal efficiency of all samples was 0.77% or less without addition of an external electron ($e^-$) donor. However $ClO_4^-$-removal efficiency was higher when an $e^-$ donor (acetate, thiosulfate, $S^0$, or $F^0$) was added into the batch culture, showing up to 100% removal efficiency. The removal efficiency was various depending on type of $e^-$ donor and site of sampling. When acetate was used as an $e^-$ donor, the highest $ClO_4^-$-removal efficiency was observed among the $e^-$ donors used in this study, suggesting that activity of heterotrophic PRB was dominant. The results of this study provide basic information on natural attenuation of $ClO_4^-$ by river microorganisms. The information can be useful to prepare a strategy to enhance efficiency of $ClO_4^-$ biodegradation for in situ bioremediation.

Generating global warming scenarios with probability weighted resampling and its implication in precipitation with nonparametric weather generator

  • Lee, Taesam;Park, Taewoong
    • Proceedings of the Korea Water Resources Association Conference
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    • 2015.05a
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    • pp.226-226
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    • 2015
  • The complex climate system regarding human actions is well represented through global climate models (GCMs). The output from GCMs provides useful information about the rate and magnitude of future climate change. Especially, the temperature variable is most reliable among other GCM outputs. However, hydrological variables (e.g. precipitation) from GCM outputs for future climate change contain too high uncertainty to use in practice. Therefore, we propose a method that simulates temperature variable with increasing in a certain level (e.g. 0.5oC or 1.0oC increase) as a global warming scenario from observed data. In addition, a hydrometeorological variable can be simulated employing block-wise sampling technique associated with the temperature simulation. The proposed method was tested for assessing the future change of the seasonal precipitation in South Korea under global warming scenario. The results illustrate that the proposed method is a good alternative to levy the variation of hydrological variables under global warming condition.

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Study on Pursuit of Contamination Sources and Establishment of Sanitary Standard from Raw Pork Meat (원료돈육의 오염원 추적 및 위생기준 설정에 관한 연구)

  • 임대석;강희곤;김용곤;김창한
    • Food Science of Animal Resources
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    • v.21 no.2
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    • pp.149-155
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    • 2001
  • The aims of this study were to examine contamination sources and provide the basic data in establishment of sanitary standard for raw pork meat. From \"Random sampling(I)\", initial total plate counts of post-slaughter samples for the group A, B and C were 1.5${\times}10^4$cfu/$textrm{cm}^2$, 5.5${\times}10^5$cfu/$textrm{cm}^2$ and 1.8${\times}10^4$cfu/$textrm{cm}^2$, respectively, and of post-prechilling samples for the group A, B and C were 1.0${\times}10^4$cfu/$textrm{cm}^2$, 4.6${\times}10^5$cfu/$textrm{cm}^2$ and 2.5${\times}10^4$cfu/$textrm{cm}^2$, respectively. Initial total plate counts of post-transportation samples for the group D, E and F did not increased, as did the group A, B and C. From \"Normal sampling(II)\", initial total plate counts of post-slaughter, post-prechilling, post-transportation and post-2 days preservation samples were 7.3${\times}10^4$cfu/$textrm{cm}^2$, 9.6${\times}10^4$cfu/$textrm{cm}^2$, 2.0${\times}10^5$cfu/$textrm{cm}^2$ and 2.5${\times}10^5$cfu/$textrm{cm}^2$, respectively. From \"Clean sampling(III)\", initial total plate counts of post-slaughter, post-prechilling, post-transportation and post-2 days preservation samples were decreased to 7.0${\times}10^2$cfu/$textrm{cm}^2$, 7.5${\times}10^2$cfu/$textrm{cm}^2$, 8.5${\times}10^2$cfu/$textrm{cm}^2$ and 5.5${\times}10^3$cfu/$textrm{cm}^2$, respectively, compared with "Normal sampling(II)". No E. coli O157:H7, Staphylococcus aureus and Salmonella were detected at each sampling step. Consequently, a slaughter method like "Clean sampling(III)" showed a better sanitary effect to low total plate counts of 10$^2$∼10$^3$times, compared with "Normal sampling(II)". The one of contamination sources for raw pork meat was at a slaughtering step, and "Clean sampling" method may be considered as the one of sanitary standards.

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Resistance Patterns of Frequently Applied Antimicrobials and Occurrence of Antibiotic Resistance Genes in Edwardsiella tarda Detected in Edwardsiellosis-Infected Tilapia Species of Fish Farms of Punjab in Pakistan

  • Kashif Manzoor;Fayyaz Rasool;Noor Khan;Khalid Mahmood Anjum;Shakeela Parveen
    • Journal of Microbiology and Biotechnology
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    • v.33 no.5
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    • pp.668-679
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    • 2023
  • Edwardsiella tarda is one of the most significant fish pathogens, causes edwardsiellosis in a variety of freshwater fish species, and its antibiotic resistance against multiple drugs has made it a health risk worldwide. In this study, we aimed to investigate the antibiotic resistance (ABR) genes of E. tarda and establish its antibiotic susceptibility. Thus, 540 fish (299 Oreochromis niloticus, 138 O. mossambicus, and 103 O. aureus) were collected randomly from twelve fish farms in three districts of Punjab in Pakistan. E. tarda was recovered from 147 fish showing symptoms of exophthalmia, hemorrhages, skin depigmentation, ascites, and bacteria-filled nodules in enlarged liver and kidney. Antimicrobial susceptibility testing proved chloramphenicol, ciprofloxacin, and streptomycin effective, but amoxicillin, erythromycin, and flumequine ineffective in controlling edwardsiellosis. Maximum occurrence of qnrA, blaTEM, and sul3 genes of E. tarda was detected in 45% in the liver, 58%, and 42% respectively in the intestine; 46.5%, 67.2%, and 55.9% respectively in O. niloticus; 24%, 36%, and 23% respectively in summer with respect to fish organs, species, and season, respectively. Motility, H2S, indole, methyl red, and glucose tests gave positive results. Overall, E. tarda infected 27.2% of fish, which ultimately caused 7.69% mortality. The Chi-squared test of independence showed a significant difference in the occurrence of ABR genes of E. tarda with respect to sampling sites. In conclusion, the misuse of antibacterial agents has led to the emergence of ABR genes in E. tarda, which in association with high temperatures cause multiple abnormalities in infected fish and ultimately resulting in massive mortality.