• 한국식품영양학회지
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• 제10권1호
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• pp.122-126
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• 1997

Bifidobacteria have been known as beneficial inhabitant of human intestine. Therefore, bifidobacteria began to be noticed as a starter in the manufacture of fermented dairy products. Perhaps the key for effective use of bifidobacteria in commercial dairy products is the maintenance of viability of bifidobacteria during large scale preparation of starter culture and distribution of products. So we tried to obtain the bifidobacteria having suitable characteristics for using as a starter in the manufacture of fermented dairy products. Among bifidobacteria isolated from Korean, E-4 strain showed the highest resistance to oxygen. To know whether the selected strain will be fit for manufacture of fermented dairy products, we also investigated resistance of the selected strain to HCI. The selected strain, E-4, was more resistant to environmental stresses such as oxygen, H2O2 and HCI than Bifidobacterium longum known as resistant strain to environmental stresses. According to carbohydrate fermentation patterns and morphological characteristics, E-4 strain was identified as B. bifidum. In conclusion, the selected strain, E-4, was thought to be fit for manufacture of fermented dairy products.

• Journal of Dairy Science and Biotechnology
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• 제39권3호
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• pp.113-120
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• 2021

Although the number of incidences of illness caused by ingestion of the bacterial pathogen Enterobacter sakazakii (Cronobacter spp.) has dramatically declined, there remains a need for a robust isolation method to recover this microbe from powdered infant formula (PIF). The current method described in the FDA's Bacteriological Analytical Manual requires multiple steps, and 3-4+ days for complete analysis of PIF isolated E. sakazakii (Cronobacter spp.). We describe a bacteriological method including a one-step enrichment followed by plating on chromogenic agar for presumptive identification of E. sakazakii (Cronobacter spp.). Suspected colonies are confirmed by either biochemical analyses, or a Real-Time PCR-based assay. Using this method, E. sakazakii (Cronobacter spp.) in PIF can be isolated and identified within one day (24 hours).

• Journal of Applied Biological Chemistry
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• 제54권3호
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• pp.159-165
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• 2011

로즈마리 추출물의 phenol 함량은 25.7 mg/g으로 매우 높게 나타났다. Helicobacter pylori 에 대한 항균효과를 측정한 결과, clear zone이 $100{sim}200{\mu}/disc$의 phenol 농도에서 각각 11, 12, 14 mm의 clear zone을 형성하여 추출물의 농도가 증가할수록 H. pylori 에 대한 저해효과가 농도 의존적으로 높아지는 것을 확인 할 수 있었다. Sephadex LH-20과 MCI-gel CHP-20 column을 이용하여 normal phase type인 $ethanol{\rightarrow}H_2O$와 reverse phase type인 $H_2O{\rightarrow}methanol$로 용액의 농도를 증가시키면서 용출한 결과 H. pylori에 대한 탁월한 저해활성을 나타낸 5종류(compound A~E)의 단일 물질로 분리하였다. 5가지 각각의 물질은 H. pylori에 대한 항균력이 미약하였으나, 2종류 이상의 다중 물질의 항균 시너지 효과에 의해 각각 10~16 mm의 clear zone을 형성하여 우수한 항균력을 나타내었으며, fast atom bombardment (FAB)-Mass, $^1H$-NMR, $^{13}C$-NMR과 IR spectrum을 사용하여 구조 동정한 결과 protocatechuic acid, coumaric acid, caffeic acid, chlorogenic acid 및 rosmarinic acid 인 것으로 확인되었다.

• 한국전자파학회논문지
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• 제13권7호
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• pp.613-624
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• 2002

본 논문에서는 Push-Push FET유전체 공진기 발진기를 20 GHz에서 설계, 제작하고 출력단에 각각 Wilkinson 전력결합기와 T 접합 전력결합기를 사용하였을 때의 각 결합기의 반사손실과 격리도 특성에 따른 발진기의 출력특성을 조사하여 이들 특성이 출력과 위상잡음 특성에 각각 영향을 주는 것을 설명한다. 기본 주파수 10 GHz를 억제하고 제 2고조파 주파수를 이용하는 20 GHz Push-Push발진기는 T $E_{01}$$\delta$/모드의 유전체 공진기와 GaAs MESFET를 높이 H = 20 mil($\varepsilon_{{\gamma}}$/=2.52) 테프론 기판 위에 장착하는 구조로 설계하고 제작하였다. 기본주파수에서 T-접합 전력결합기는 반사손실 -12 dB, 격리도 -3.7 dB 이었고, Wilkinson 전력결합기는 반사손실 -14 dB, 격리도 -11 dB 이었다. 그리고 제 2 고조파 주파수에서 T-접합 전력결합기는 반사손실 -10 dB, 격리도 -7.5 dB 이었고, Wilkinson 전력 결합기 는 반사손실 -23 dB, 격리도 -22 dB를 보였다. 결과적으로 반사손실과 격리도 특성이 좋은 Wilkinson 전력 결합기를 출력 단으로 이용한 Push-Push 발진기 가 출력전력레벨면이나 위상잡음특성면에서 T-접합 전력결합기를 이용한 발진기보다 우수한 특성을 보이는 것을 확인하였다.발진기보다 우수한 특성을 보이는 것을 확인하였다.

• 한국소음진동공학회논문집
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• 제23권10호
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• pp.902-908
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• 2013

The input torque ripple induced by combustion engines is a significant source of NVH(noise, vibration and harshness) problem in automotive transmissions. Because this torque fluctuation is primarily transmitted to the input shaft of automotive powertrains(e.g., automatic transmissions) when the lock-up clutches are closed, a torsional damper with helical springs is generally inserted between engine and transmissions to isolate the input vibratory energy, which is essential for the passenger comfort. The torsional vibration isolator exhibits frequency ranges in which there is low vibration transmissibility. However, the isolation performance is currently evaluated through the static torsional spring characteristics. In this study, the transmissibility of torsional spring dampers, essential dynamic performance index for vibration isolator, is first experimentally evaluated.

• Natural Product Sciences
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• 제16권4호
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• pp.211-216
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• 2010

A new 3-indolyl propionate alkaloid; (-) abrine (1), was isolated from the alcohol extract of Erythrina caffra stem bark together with the alkaloid (-) hypaphorine (2). Phytochemical investigation of the chloroform extract led to the isolation of one fatty alcohol; 1-octacosanol, isolated for the first time from genus Erythrina, two dienoid alkaloids; erythraline and erysodine, and three isoflavonoids; 3S (+) 2'-O-methylphaseollidinisoflavan (3), (6aR, 11aR)- 3-hydroxy-10-dimethylallyl-9-methoxypterocarpan, known as sandwicensin (4) and 3R (-) rythbidin A (5). It is worth mentioning that this is the first report for the isolation of these isoflavonoids from E. caffra. The absolute configuration of 3S (+) 2'-O-methylphaseollidinisoflavan (3) was not previously reported. The isolated isoflavonoids showed promising antibacterial activity against Staphylococcus aureus.

• 한국정보통신학회논문지
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• 제6권3호
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• pp.440-444
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• 2002

Sierpinski 프랙탈 삼각형을 이용한 원형 편파를 가지는 마이크로스트립 패치 안테나를 제안한다. Sierpinski 프랙탈 삼각형은 3개의 정삼각형을 가지는 구조로 이를 이용하여 sequential 회전 기법에 의해 광대역의 원형 편파를 발생시킬 수 있다. Sierpinski 구조의 1x3 안테나 배열에 대해포트간 분리도(isolation)와 AR(Axial Ratio)의 특성을 살펴보았다.

• Structural Engineering and Mechanics
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• 제8권4호
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• pp.411-419
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• 1999

A nonlinear model for time-step analysis of bridges subjected to two orthogonal horizontal components of earthquake motions was developed. The focus of the study was on elastomeric isolators with or without lead cores. The hysteretic behavior of the isolators, the columns, abutments, and shear keys was taken into account. The nonlinear analysis showed that, contrary to linear theory prediction, the use of isolators does not necessarily increase the displacement of the superstructure. Furthermore, it was shown that properly designed isolators can reduce the ductility demand in RC bridge columns substantially.

• 전자공학회논문지D
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• 제35D권5호
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• pp.40-47
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• 1998

This paper report sthe design, fabrication and characterization of an AlGaAs/GaAs HBT (heterojunction bipolar transistor) with an air-bridge isolation structure which is made to improve high frequency characteristics for the application to the mobile communication system in the next genration. We found that the size, shape and structure of HBT have an effect on the high frequency operation. The measured dc and ac characteristics of the four type HBTs were compared and analyzed. An E-type HBT with an air-bridge structure by undercut etching exhibited .beta.=56, $V_{off-set}$ = 0.3 V, B $V_{CEO}$=7.0V with $f_{T}$=40 GHz and $f_{max}$=45GHz at a collector current density of 7.1*10$^{4}$ A/c $m^{2}$.>.

• 한국토양비료학회지
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• 제48권2호
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• pp.119-124
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• 2015

Pyoverdines (PVDs) are organic compounds produced by the fluorescent Pseudomonads under iron starvation conditions. Among the isolated rhizosphere pseudomonads strains, P. putida KNUK9 showed the highest production of PVDs and its production reached to 62.81% siderophores units. DNA isolation, ligation, PCR amplification, and transformation using E. coli $DH5{\alpha}$ cells were carried out for preparing the strong pyoverdine producer strains. We detected seven genes playing the fundamental roles in the pyoverdine metabolism in Pseudomonads. According to data and analysis obtained from the study, we deduced that the strain P. putida KNUK9 contains the essential genes required for pyoverdine biosynthesis.