• Journal of Marine Bioscience and Biotechnology
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• v.12 no.1
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• pp.50-58
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• 2020

Sphacelaria is a filamentous brown algal genus that can be epibiotic on macroalgae, marine plants, and sea turtles. Its important role in benthic ecosystems, exposure to different stressors (e.g., grazing), and use as a model organism make Sphacelaria ideal for assessing physiological responses of organisms to environmental inputs. Single-cell RNA sequencing is a powerful new probe for understanding environmental responses of organisms at the molecular (transcriptome) level, capable of delineating gene regulation in different cell types. In the case of plants, this technique requires protoplasts ("naked" plant cells). The existing protoplast isolation protocols for Sphacelaria use non-commercial enzymes and are low-yielding. This study is the first to report the production of protoplasts from Sphacelaria fusca (Hudson) S.F. Gray, using a combination of commercial enzymes, chelation, and osmolarity treatment. A simple combination of commercial enzymes (cellulase Onozuka RS, alginate lyase, and driselase) with chelation pretreatment and an increased osmolarity (2512 mOsm/L H₂O) gave a protoplast yield of 15.08 ± 5.31 × 10⁴ protoplasts/g fresh weight, with all the Sphacelaria cell types represented. Driselase had no crucial effect on the protoplast isolation. However, the increased osmolarity had a highly significant and positive effect on the protoplast isolation, and chelation pretreatment was essential for optimal protoplast yield. The protocol represents a significant step forward for studies on Sphacelaria by efficiently generating protoplasts suitable for cellular studies, including single-cell RNA sequencing and expression profiling.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2007.05a
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• pp.97-102
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• 2007

As environmental vibration requirements on precision equipments get more stringent, use of pneumatic vibration isolators becomes more crucial and, hence, their dynamic performance needs to be further improved. Dynamic behavior of those pneumatic vibration isolation tables is very important to both manufacturer and customer as performance specifications. Together with conventional transmissibility, transient response characteristics are another critical performance index especially when movements of components, e.g., x-y tables, of the precision equipments are very dynamic. In this paper, analysis on transient response of a pneumatic vibration isolation table loaded by a mass moving on it is presented. This is a conventional dynamics problem on a rigid body with 6 degree of freedom and a mass with another degree of freedom. How to obtain transient responses of the isolation table is described when the movements of the mass are prescribed relative to the table.

• Journal of the Korean Society of Environmental Restoration Technology
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• v.13 no.5
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• pp.146-153
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• 2010

Urban development typically results in many species being confined to small, isolated and degraded habitat fragments. Fragment size and isolation underpin many studies of modified landscape to prevent biodiversity loss. However, habitat characteristics such as vegetation structure and edge effects are less frequently incorporated in planning. The relative influence of biogeographic (e.g. size, isolation) and vegetation parameters on assemblages is poorly understood, but critical for conservation management. In this study, the relative importance of biogeographic and vegetation parameters in explaining the diversity of forest-interior dwelling birds in forest fragments in Chungcheong Province Area. Fragment size and vegetation characteristics were consistently important predictors of bird diversity. Forestinterior bird richness was influenced by fragment size (0.437), wood age (0.332), wood diameter (0.068), and patch shape (-0.079). To preserve bird diversity of Chungcheong Province Area, it is important to consider differing responses of bird diversity to landscape change, move beyond a focus primarily on spatial attributes (size, isolation) to recognize that landscape change also has profound effects on habitat composition and quality. The result is very useful for long-term aspect of biodiversity conservation plan in regional scale.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.17 no.6 s.123
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• pp.515-523
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• 2007

As environmental vibration requirements on precision equipments get more stringent, use of pneumatic vibration isolators becomes more crucial and, hence, their dynamic performance needs to be further improved. Dynamic behavior of those pneumatic vibration Isolation tables is very important to both manufacturer and customer as performance specifications. Together with conventional transmissibility, transient response characteristics are another critical performance index especially when movements of components, e.g., x-y tables, of the precision equipments are very dynamic. In this paper, analysis on transient response of a pneumatic vibration isolation table loaded by a mass moving on it is presented. This is a conventional dynamics problem on a rigid body with 6 degree of freedom and a mass with another degree of freedom. How to obtain transient responses of the isolation table is described when the movements of the mass are prescribed relative to the table.

• Korean Journal of Microbiology
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• v.49 no.2
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• pp.179-183
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• 2013

Marine-derived microbes have yielded a variety of metabolites so far. In the course of the project to find metabolites from marine microbes, an isolate of Aspergillus protuberus (SF 5767) was selected for chemical investigation. A large scale culture of this strain in PDA media was extracted with an organic solvent and the extract was fractionated by silica gel column chromatography. Repeated reverse phase HPLC of the fractions led to the isolation of three metabolites. Their chemical structures were elucidated as deoxybrevianamide E (1), brevianamide V (2), and ergosterol peroxide (3) on the basis of spectroscopic data including MS, NMR, and UV. To the best of our knowledge, chemical investigation of A. protuberus was conducted for the first time in this study.

• Korean Journal of Veterinary Research
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• v.36 no.2
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• pp.371-378
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• 1996

Three and 2 strains of E coli O157 were isolated from fecal materials of cattle (390) and pigs (420) in Korea, respectively. One strain of O157:H7 isolated from cattle and 2 strains of O157:H7 isolated from pigs were identified as verotoxin-1 (VT-1) produing strains and 2 strains (O157:H7 and O157:H-) isolated from cattle were identified as verotoxin-2 (VT-2) producing strains by neutralization test on HeLa and Vero cells. Culture supernatants of the isolates were cytotoxic to HeLa and Vero cells. The levels of cytotoxin produced by isolates were $10^2{\sim}10^4$ cytotoxic dose($CD_{50}$)/ml. Also, VT-2-converting bacteriophage was isolated from KSC109 strain which had been isolated from cattle. Molecular weight of the phage DNA was determined as approximately 45 Kb in 0.8% agarose gel electrophoresis, and morphology of the phage stained with phosphotungstic acid was observed by transmissible electron microscopy.

• Journal of Life Science
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• v.8 no.6
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• pp.711-722
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• 1998

This study was undertaken to determine the antimicrobial susceptibilities of 716 bacterial strains among 1,104 microbes which were isolated in blood culture from 13,595 in-patients and out-patients in K hospital during the pe-riod of 1993 to 1996. The results obtained were as follows : The isolation rate of microbes from the blood culture for 3 years was 8.1% (1,104/13,595). Among 716 bacte-rial strains, Escherichia coli and Staphylococcus epidermidis were 29.8% (213) and 19.5% (140) respectively. The bacterial isolation frequency according to ages was high at fifties, sixties and two or less years old groups, especially the isolation rate of S. epidermidis was 47% at two or less years old group and that of Salmonella typhi was 36% at thirties years old group. The seasonal isolation rate of the bacterial species except E. coli was high during May to September. Gram positive cocci were resistant to penicillin-G except Enterococcus faecalis, but susceptible to vanco-mycin and teicoplanin. Gram negative bacilli were susceptible to amikacin, ciprofloxacin, and imipenem, but resistant to ampicillin except S. typhi. These results indicate that bacterial strains isolated from bacteremia patients were resis-tant to penicillins, but susceptible to vancomycin, teicoplanin, amikacin, ciprofloxacin and imipenem.

• Smart Structures and Systems
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• v.25 no.3
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• pp.323-335
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• 2020

Magnetorheological elastomer (MRE), a smart material, is an innovative material for base isolation system. It has magnetorheological (MR) effect that can control the stiffness in real-time. In this paper, a new hybrid base isolation system combining two electromagnetic closed circuits and the roller bearing is proposed. In the proposed system, the roller part can support the vertical load. Thus, the MRE part is free from the vertical load and can exhibit the maximum MR effect. The MRE magnetic loop is constructed in the free space of the roller bearing and forms a strong magnetic field. To demonstrate the performance of the proposed hybrid base isolation system, dynamic characteristic tests and performance evaluation were carried out. Dynamic characteristic tests were performed under the extensive range of strain of the MRE and the change of the applied current. Performance evaluation was carried out using the hybrid simulation under five earthquakes (i.e., El Centro, Kobe, Hachinohe, Northridge, and Loma Prieta). Especially, semi-active fuzzy control algorithm was applied and compared with passive type. From the performance evaluation, the comparison shows that the new hybrid base isolation system using fuzzy control algorithm is superior to passive type in reducing the acceleration and displacement responses of a target structure.

• Earthquakes and Structures
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• v.4 no.6
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• pp.587-606
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• 2013

The given paper presents a new approach in design of seismic isolation systems of base isolated buildings. The idea is to install not one big size rubber bearing under the columns and/or shear walls, or one by one with certain spacing under the load-bearing walls, but to install a group/cluster of small size bearings, in order to increase the overall effectiveness of the isolation system. The advantages of this approach are listed and illustrated by the examples. Also the results of analyses of some buildings where the approach on installation of clusters of rubber bearings was used in their isolation systems are given for two cases: i) when the analyses are carried out based on the provisions of the Armenian Seismic Code, and ii) when the time history analyses are carried out. Obtained results are compared and discussed. Paper also presents, as an example, detailed analysis and design of the 18-story unique building in one of the residential complexes in Yerevan. Earthquake response analyses of this building were carried out in two versions, i.e. when the building is base isolated and when it is fixed base. Several time histories were used in the analyses. Comparison of the obtained results indicates the high effectiveness of the proposed structural concepts of isolation systems and the need for further improvement of the Seismic Code provisions regarding the values of the reduction factors. A separate section in the paper dedicated to the design of high damping laminated rubber-steel bearings and to results of their tests.

• Korean Journal of Food Science and Technology
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• v.51 no.6
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• pp.604-609
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• 2019

To control pathogenic E. coli in biofilm, bacteriophages were isolated from environmental samples. Seventeen isolates had depolymerase activities by translucent zones at the rims of plaques. To determine biofilm-forming ability, an abiotic plastic surface of polystyrene was used; E. coli O103 showed the highest biofilm formation at 30℃ after 24 h. Moreover, biofilm by E. coli O103 on the biotic surface of lettuce was observed by a scanning electron microscope. The bacteriophage cocktail of ΦNOECP40 and ΦNOECP44 showing depolymerase activities was prepared to eliminate the E. coli inbiofilm. By organic acids, reduction of E. coli in biofilm was insignificant and almost undetectable. However, the abundance of E. coli in biofilm was reduced by 3 log CFU/mL from 7.3 log CFU/mL after 60 min with the bacteriophage cocktail. Therefore, we suggest that bacteriophages with depolymerase could be utilized to effectively control pathogenic E. coli in biofilm.