• Title/Summary/Keyword: E-64

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Mechanism of Glycogen Biosynthesis by Glycogen Synthetase from Escherichia coil B (Escherichia coil B의 Glycogen Synthetase반응에 의한 글리코겐 생합성기작)

  • 양지영
    • Journal of the Korean Society of Food Science and Nutrition
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    • v.27 no.6
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    • pp.1173-1176
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    • 1998
  • Glycogen synthetase[EC 2.4.1.21] in E. coli B was isolated and purified by sonication, ultracentri fugation, DEAE cellulose chromatography and gel chromatography. In the case of using glycogen or maltotriose as a primer in the enzyme reaction, 64% and 23.7% of labelled ADP glucose were incorporated into primer, respectively. 8.1% of labelled ADP glucose was polymerized into glycogen in enzymatic reaction without a primer.

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CPQRA를 이용한 위험물질의 누출에 따른 독성인자평가

  • 이수길;이내우;최재욱;설수덕
    • Proceedings of the Korean Institute of Industrial Safety Conference
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    • 1997.11a
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    • pp.59-64
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    • 1997
  • 화학관련분야의 공정안전에 관하여 오랜 역사를 가진 AIChE는 관련산업분야의 안전규정을 예방적인 측면과 사고원인을 규명하기 위한 측면에서 근원적인 자료를 제시하였고 1955년 AIChE에 의해 설립된 CCPS(center for chemical process safety)는 이에 관한 기술적인 면에 더욱 발달된 정보를 제공하게 되었다. 이런 정보들 가운데 사업장의 위험물질이 지니고 있는 위험성을 어떻게 평가할 것인가에 관하여 CPQRA(chemical process quantitative risk analysis)방법이 제시되어 있다. 1) CPQRA는 양적인 의미에서 위험성의 정의, 분석, 평가, 통제 및 관리방법 등에 대비해 잠재적인 방법을 제시한 것이다. (중략)

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A DESIGN AND DEVELOPMENT OF MULTI-PURPOSE CCD CAMERA SYSTEM WITH THERMOELECTRIC COOLING II. SOFTWARE (열전냉각방식의 범용 CCD 카메라 시스템 개발 II. 소프트웨어)

  • Oh, S.H.;Kang, Y.W.;Byun, Y.I.
    • Journal of Astronomy and Space Sciences
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    • v.24 no.4
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    • pp.367-378
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    • 2007
  • We present a software which we developed for the multi-purpose CCD camera. This software can be used on the all 3 types of CCD - KAF-0401E ($768{\times}512$), KAF-1602E ($1536{\times}1024$), KAF-3200E ($2184{\times}1472$) made in KODAK Co.. For the efficient CCD camera control, the software is operated with two independent processes of the CCD control program and the temperature/shutter operation program. This software is designed to fully automatic operation as well as manually operation under LINUX system, and is controled by LINUX user signal procedure. We plan to use this software for all sky survey system and also night sky monitoring and sky observation. As our results, the read-out time of each CCD are about 15sec, 64sec, 134sec for KAF-0401E, KAF-1602E, KAF-3200E., because these time are limited by the data transmission speed of parallel port. For larger format CCD, the data transmission is required more high speed. we are considering this control software to one using USB port for high speed data transmission.

Effects of Jasmonic Acid on Root Growth and Eleutheroside Accumulation in Adventitious Root Culture of Eleutherococcus koreanum (섬오갈피나무 부정근(不定根) 배양(培養)에서 부정근(不定根)의 생장(生長)과 Eleutheroside 류(類)의 생산(生産)에 미치는 Jasmonic acid 처리(處理)의 영향)

  • An, Jin-Kwon;Park, So-Young;Lee, Wi-Young;Park, Yeongki
    • Journal of Korean Society of Forest Science
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    • v.95 no.1
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    • pp.32-37
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    • 2006
  • The influence of different doses of jasmonic acid on both biomass of adventitious roots and accumulation of secondary metabolite in bioreactor cultures of Eleutherococcus koreanum was studied. The maximum growth (2.9 g Dry Weight/L) of adventitious roots was observed in the absence of jasmonic acid. The biomass of adventitious roots decreased with the increase in jasmonic acid dosage. High level of jasmonic acid efficiently stimulated the production of both eleutheroside B and E. The highest eleutheroside B ($49.0{\mu}g/g\;DW$) was observed at 0.4 mg/L jasmonic acid. Jasmonic acid at 0.2 mg/L led to the maximum accumulation of eleutheroside E ($64.7{\mu}g/g\;DW$). Whereas eleutheroside $E_1$ was maximally $19.0{\mu}g/g\;DW$ accumulated in the absence of jasmonic acid, the total eleutheroside ($259.9{\mu}g/L$) was the highest when 0.01 mg/L jasmonic acid was treated. In this study, the level of eleutheroside B decreased whereas the maximum levels of eleutheroside E and eleutheroside $E_1$ were observed at 10th and 4th day after jasmonic acid treatment.

Distributional Characteristics of Escherichia coli at Nakdong River Mouth and Busan Coastal Area (낙동강 하구와 부산연안해역에서 대장균의 해역별 분포특성)

  • Baek, Seung Ho;Lee, Min Ji;Yoon, Dongyoung
    • Journal of Marine Life Science
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    • v.1 no.1
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    • pp.1-7
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    • 2016
  • In order to assess seasonal and geographical characteristics of pollutant Escherichia coli, we investigated its distribution in Nakdong River mouth and Busan coastal water from February 2013 to November 2015. The coastal area was divided into five different zones (I-V) based on the pollutant level and geographical characteristics. During the study periods, water temperature and salinity varied from 7.50 to 27.64℃ and 16.82 to 34.82 psu, respectively. The annual water temperature variation was characterized in temperate zone. The salinity was significantly (p<0.05) decreased in zone IV and zone III after heavy rain during summer season in 2014, resulting led to elevated E. coli biomass. The highest colony formation of E. coli was recorded at 6,000 cfu l-1 during autumn at station 1 (zone I). On the other hands, during all seasons of 2015, E. coli abundances were kept to be low level in zone III. The E. coli was not significantly (p>0.05) correlated with water temperature. However, the salinity was significantly (r=-0.53, p<0.05) correlated with the E. coli, implying that salinity plays a crucial role in the proliferation of E. coli. Consequently, E. coli in western Busan coastal water might have been significantly promoted by pollutant sources from Nakdong Rive discharge during the spring and summer rainy seasons depending on annual rainfall variations. On the other hands, E. coli in station 1 (i.e., Suyeong Bay) was obviously high due to influences of discharge water from municipal wastewater treatment plant. However, there was no clear seasonality of E. coli.

Identification of the Transmissive Fractures in the Vicinity of waterway Tunnel (도수로터널 주변 지역의 지하수 유동성 단열 규명)

  • 이병대;이인호;추창오;함세영;성익환;황세호
    • Journal of Soil and Groundwater Environment
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    • v.7 no.3
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    • pp.33-44
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    • 2002
  • A field technique for assessing the transmissive fractures in an aquifer was applied to a fractured rock formation in Youngchun area Korea. Geological mapping and detailed acoustic borehole teleview(BHTV) logging were performed to obtain information about the fractures. The study area consists predominantly of two types of fractures. The fracture sets of low angle partings such as bedding and sheeting plains have strike N70-80$^{\circ}$W, 25$^{\circ}$-30$^{\circ}$SW and N3S$^{\circ}$W, 12$^{\circ}$NE, respectively. In areas of high fractures, on the other hand, the major fracture sets show strike N80$^{\circ}$W and dip 70$^{\circ}$-85$^{\circ}$SW, N10$^{\circ}$E.85$^{\circ}$SE in sedimentry rocks, N40-50$^{\circ}$E.85$^{\circ}$SE/85$^{\circ}$NE, N70$^{\circ}$E.80$^{\circ}$SE, and N7$^{\circ}$-75$^{\circ}$W.80$^{\circ}$SW in granites and volcanic rocks. Injection tests have been performed to identify discrete production zones and quantify the vertical distribution of hydraulic conductivity. The calculated hydraulic conductivities range from 3.363E-10 to 2.731E-6, showing that the difference between maximum and minimum value is four order of magnitude. Dominant section in hydraulic conductivity is extensively fractured. Geophysical logging was carried out to clarify characterization of the distribution of fracture zones. Transmissive fractures were evaluated through the comparison of the results obtained by each method. The temperature logs appeared to be a good indicator that can distinguish a high transmissive fractures from a common fractures in hydraulic conductivity. In numerous cases, evidence of fluid movement was amplified in the temperature gradient log. The fracture sets of N70-80$^{\circ}$W.60-85$^{\circ}$NE/SW N75-80$^{\circ}$W.25-30$^{\circ}$SW, N50-64$^{\circ}$W.60-85$^{\circ}$NE, N35-45$^{\circ}$E.65-75$^{\circ}$SE, and N65-72$^{\circ}$E.80$^{\circ}$SE/60$^{\circ}$NW were idenfied as a distinct transmissive fractures through the results of each tests.

Hemispherical Grained Silicon formation Condition on In-Situ Phosphorous Doped Amorphous-Si Using The Seeding Method (Seeding Method를 이용한 인이 도우핑된 Amorphous-Si에서의 HSG형성 조건)

  • 정양희;강성준
    • Journal of the Korea Institute of Information and Communication Engineering
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    • v.5 no.6
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    • pp.1128-1135
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    • 2001
  • In this paper, a new HSG-Si formation technology, "seeding method', which employs Si$_2$H$_{6}$-molecule irradiation and annealing, was applied for realizing 64Mbit DRAMs. By using this technique, grain size controlled HSG-Si can be fabricated on in-situ phosphorous-doped amorphous-Si electrode. The new HSG-Si fabrication technology achieves twice the storage capacitance with high reliability for the stacked capacitors. In this technique, optimum process conditions of the phosphorous concentration, storage polysilicon deposition temperature and thickness of hemispherical grain silicon are in the range of 3.0-4.0E19atoms/㎤, 53$0^{\circ}C$ and 400$\AA$, respectively. In the 64M bit DRAM capacitor using optimum process conditions, limit thickness of dielectric nitride is about 65$\AA$.

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Evaluation of Usefulness of an m-DIXON Technique during an Abdomen MRI Examination : A Comparison with an e-THRIVE Technique (복부 MRI검사에서 m-DIXON기법의 유용성 평가: e-THRIVE기법과 비교 분석)

  • Lee, Bo-Woo;Park, Myung-Cheol;Lee, Jin-Hoi;Kim, Ki-Jin;Bae, Seok-Hwan
    • Journal of Digital Convergence
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    • v.12 no.10
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    • pp.385-390
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    • 2014
  • This research is conducted to identify whether an m-DIXON technique which is useful for an abdomen MRI examination compared with an existing e-THRIVE technique is a clinically useful or not. There was evaluated quantitative and qualitative to 84 subjects who had abdomen MRI exam due to their liver disease were conducted during a period from September in 2013 to February in 2014. First of all the quantitative evaluation, the m-DIXON technique's SNR was $90.42{\pm}16.90$ and the e-THRIVE technique was $60.42{\pm}11.54$ and the m-DIXON technique's CNR was $52.38{\pm}22.58$ and the e-THRIVE technique was $46.31{\pm}20.25$. Secondly in the qualitative evaluation, the m-DIXON technique's image quality was $4.06{\pm}0.34$, a artifact was $3.64{\pm}0.22$, and fat suppression was $4.16{\pm}0.15$, the e-THRIVE technique's image quality was $3.14{\pm}0.35$, a artifact was $3.06{\pm}0.38$, fat suppression was $3.14{\pm}0.30$. In conclusion, m-DIXON technique for abdomen MRI examination showed superiority over both SNR as a quantitative anaylsis, CNR and a qualitative analysis.

Quality Characteristics and Distribution of Early Harvesting Mandarin Oranges (조생 감귤의 품질 특성 분포 연구)

  • 홍석인;이주원;김선희;정문철;박형우;김동만
    • Food Science and Preservation
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    • v.10 no.1
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    • pp.54-59
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    • 2003
  • To get basic data for quality grading of Mandarin oranges, the oranges were collected from five major areas distributed in Cheju Island, Korea for 3 years and the quality characteristics such as greenness, size, weight, uniformity, sweetness, pH and titratable acidity and its distributions were analyzed. The mean value of greenness (100- yellowness) of peel color in Mandarin oranges was 2.58% and its variation was very large, 9.04%. The average height and diameter of the oranges were 47.51 mm and 56.05 mm, respectively. The average weight was 82.24 g and its variation was 29.92g. The sweetness varied between 5.60$^{\circ}$Brix and 17.2$^{\circ}$Brix and its average value was 10.53$^{\circ}$Brix. The average values of the pH and total acidity of the oranges were 3.90 and 0.71 %, individually. In quality characteristics of Mandarin oranges according to the cultivated areas, area D had the lowest value in the strength of greenness, 1.14% and area D had the highest value, 3.53%. The highest values of the height and diameter in Mandarin oranges were 49.35mm and 57.48mm for area A and the lowest values of them were 46.35mm and 54.87mm for area E, respectively. The highest value of the weight in Mandarin oranges was 87.61 g for area A and the lowest value was 76.77g fer area E. Although the sweetness ranged from 10.2$^{\circ}$Brix for area E to 10.75$^{\circ}$Brix for area D, the difference by the areas was insignificant. For pH values, area B had the highest value, 3.96, while area E showed the lowest value, 3.78. Area A and E in titratable acidity were the areas shown the highest value, 0.75%, whereas area B marked the lowest value, 0.64%.

Relationship between Moxifloxacin Resistance Pheno- and Genotype of Moxifloxacin-Resistant Mycoplasma hominis Obtained in vitro (인위적으로 유도된 목시플로사신 내성 Mycoplasma hominis의 표현형과 유전자형의 연관성)

  • Park, In-Dal;Choi, Myung-Won
    • Journal of Life Science
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    • v.20 no.10
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    • pp.1544-1548
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    • 2010
  • Moxifloxacin (MF) - resistant mutants of Mycoplasma hominis (M. hominis) were generated by stepwise selection in increasing concentrations of MF, and six strains of MF resistant M. hominis mutants - M1, M4, M8, M16, M32, and M64 - in which MICs of MF were 0.5, 4, 8, 16, 32, 64 ${\mu}g$/ml, respectively, were generated. Compared to the sequence of M. hominis PG21, all mutants harbored amino acid substitutions of Arg-163 Thr in GyrA, and Pro-445 Gln in ParE. While the concentrations were getting higher, an additional amino acid substitution was found at Ser-153 Lys in GyrA (${\geq}4{\mu}g/ml$), Ser-91 Ile in ParC (${\geq}16{\mu}g/ml$), and Val-450 Phe (${\geq}64{\mu}g/ml$) in GyrB. These substitutions seem to have an impact on resistance to MF, and GyrB change was found only in the highest concentration and seems to be associated with high-level resistance to MF. This, as far as we know, is the first description of a relationship between MF resistance phenotype and genotype.