• 한국보건간호학회지
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• 제10권2호
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• pp.51-60
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• 1996

This study was conducted to provide basic knowledges of effective environmental control of patients with allergic rhinitis. From July to October 1995, 58 dust samples Were drawn from the bedrooms of patients with allergic rhinitis who were registerd at an allergy clinic of a hospital. Those samples were examined for the amount of house mite allergens & dusts. The data were analysed by using ANOVA and Pearson correiation coefficients. The results were as follows : 1. As for the amount of house mite allergens in terms of environmental factors, the amount of house mite allergens of using washing water temperature of bedding above than $55^{\circ}C$ was significantly lower than that of below $54^{\circ}C$. Other environmental factors such as type of house, area of bed room floor, bed in bedroom, bedroom cleaning by vacuum cleaner, days after bedding washing, relative humidity of bedroom were relate to the amount of house mite allergens. 2. As for the amount of dusts in terms of environmental factors, the amount of dusts of days of days after bedding washing more than 15 days was significantly lower than thant of less than 15days. Other environmental factors such as type of house, area of bedroom floor, bed in bedroom, bedroom cleaning by vacuum cleaner, was hing water taemperature of bedding, relative humidity of bedroom were not relate to the amount of dusts. 3. There was a significant positive correlation between the amount of house mite allergens and the amount of dusts. It may be conclusively said. the amount of dusts and house mite allergens were closely associated with the washing temperature and days after washing of bedding. Hence. intensive instruction for the methods of bedding washing was needs of the patients with allergic rhinitis.

• Parasites, Hosts and Diseases
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• 제48권2호
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• pp.151-155
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• 2010

Allergen extracts from dust mites and cockroaches commonly found in Korean homes were used to evaluate their enzymatic activity as they are believed to influence allergenicity. Allergen extracts were prepared from 3 dust mite species (Dermatophagoides farinae, D. pteronyssinus, and Tyrophagus putrescentiae) and 3 cockroach species (Blattella germanica, Periplaneta americana, and P. fuliginosa) maintained in the Korea National Arthropods of Medical Importance Resource Bank. Proteins were extracted in PBS after homogenization using liquid nitrogen. The activities of various enzymes were investigated using the API Zym system. No significant difference in phosphatase, lipase, or glycosidase activity was observed among the 6 allergen extracts, but much difference was observed in protease activity. Protease activity was assessed in more detail by gelatin zymography and the EnzChek assay. Extract from T. putrescentiae showed the highest protease activity, followed by those of the cockroach extracts. Extracts from D. farinae and D. pteronyssinus showed only weak protease activity. Gelatinolytic activity was detected mainly in a 30-kDa protein in D. farinae, a 28-kDa protein in D. pteronyssinus, a > 26-kDa protein in T. putrescentiae, a > 20-kDa protein in B. germanica, and a > 23-kDa protein in P. americana and P. fuliginosa. The information on various enzymatic activities obtained in this study may be useful for future studies. In particular, the strong protease activity found in cockroach extracts could contribute to sensitization to cockroach allergens, which is known to be associated with the development of asthma.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1998년도 춘계 학술대회지
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• pp.63-64
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• 1998

• 한국작물학회지
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• 제46권3호
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• pp.180-183
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• 2001

벼먼지응애는 벼 잎집 속에서 가해하기 때문에 발생 초기에는 육안으로 잎집의 변색을 판단하기 쉽지 않고, 밀도가 높아져 잎집이 변색 한 후에 발생을 알 수 있어, 수량 감소의 원인이 되고 있으나, 우리나라에서는 아직 벼먼지응애 방제방법 확립되지 않았을 뿐만 아니라, 피해정도도 알려지지 않았다. 따라서 1997년 10월 22일 파종하여 1997년 12월 2일 이앙 후 작물시험장 인공기상실에서 주간 26$^{\circ}C$, 야간 22$^{\circ}C$의 조건에서 생육한 벼를 피해정도에 따라 조사한 결과는 다음과 같다. 1. 벼먼지응애 발생 정도에 따라 초장 및 수수는 차이가 없었으나 영화수는 감소되었다. 2.정조 변색비율은 입집의 변색정도(1-9)가 7.3인 수원 441호는 81%, 2.8인 일품벼는 18% 이었다. 3. 등숙비율은 수원 441호에서는 피해정도가 심한 IV에서는 20.4% III은 30.8%, II는 37.6% 였으며, 일품벼는 IV는 38.9%, III은 59.7%, II는 69.5% 이었다. 4. 현미 완전미율은 무피해와 대비하여 피해정도가 IV일 때 수원 441호와 일품벼의 완전미율은 비슷하였으나, III과 II에서는 수원 441호가 일품벼보다 21.0%, 15.9%가 적었다. 5. 잎집의 변색 정도와 정조의 변색 정도는 정의 상관(r=0.892), 정조의 변색 비율에 대한 등숙율과 현미수량은 부의 상관(r=-0.929, r=-0.858)이 있었다.

• Journal of Microbiology and Biotechnology
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• 제18권2호
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• pp.314-321
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• 2008

Acaricidal effects of materials derived from Diospyros kaki roots against Dermatophagoides farinae and D. pteronyssinus were assessed using impregnated fabric disk bioassay and compared with that of the commercial benzyl benzoate. The observed responses varied according to dosage and mite species. The $LD_{50}$ values of the chloroform extract of Diospyros kaki roots were 1.66 and $0.96{\mu}g/cm^2$ against D. farinae and D. pteronyssinus. The chloroform extract of Diospyros kaki roots was approximately 15.2 more toxic than benzyl benzoate against D. farinae, and 7.6 times more toxic against D. pteronyssinus. Purification of the biologically active constituent from D. kaki roots was done by using silica gel chromatography and high-performance liquid chromatography. The structure of the acaricidal component was analyzed by GC-MS, $^1H-NMR,\;^{13}C-NMR,\;^1H-^{13}C$ COSY-NMR, and DEPT-NMR spectra, and identified as plumbagin. The acaricidal activity of plumbagin and its derivatives (naphthazarin, dichlon, 2,3-dibromo-1,4-naphthoquinone, and 2-bromo-1,4-naphthoquinone) was examined. On the basis of $LD_{50}$ values, the most toxic compound against D. farinae was naphthazarin $(0.011{\mu}g/cm^2)$ followed by plumbagin $(0.019{\mu}g/cm^2),$ 2-bromo-1,4-naphthoquinone $(0.079{\mu}g/cm^2)$, dichlon $(0.422{\mu}g/cm^2)$, and benzyl benzoate $(9.14{\mu}g/cm^2)$. Additionally, the skin color of the dust mites was changed from colorless-transparent to dark brown-black by the treatment of plumbagin. Similar results have been exhibited in its derivatives (naphthazarin, dichlon, and 2-bromo-1,4-naphthoquinone). In contrast, little or no discoloration was observed for benzyl benzoate. From this point of view, plumbagin and its derivatives can be very useful for the potential control agents, lead compounds, and indicator of house dust mites.

• Parasites, Hosts and Diseases
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• 제37권3호
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• pp.163-169
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• 1999

House dust mite allergens have been well established as sensitizing agents that are important in the induction of allergic diseases. In order to analyze epitopes of the allergen and to develop a quantitative method of the allergen exposure, monoclonal antibodies against a recombinant Der p 2 (rDer p 2), one of the major allergens of Dermatophogoides pteronyssinus, were produce. Four monoclonal antibodies produced wee species-specific and did not cross-react to the D. farinae crude extract. Two of the monoclonal antibodies were found to be IgG1 and the others were IgM. For the analysis of epitopes, a Der p 2 cDNA encoding 126 amino acids (aa) was dissected into three fragments with several overlapping peptides, A (aa residues 1-49), B (44-93), and C fragment (84-126). Three monoclonal antibodies showed reactivities to the recombinant B fragment and to the full-length rDer p 2, but one monoclonal antibody reacted only with the full-length rDer p 2. Two-site capture ELISA was developed using two different monoclonal antibodies for quantitating Der p2 in house dust. The sensitivity limit was 4ng/ml with rDer p2 and $8{\;}\mu\textrm{g}/ml$ with the d. pteronyssinus crude extract. The result suggested that the assay using monoclonal antibodies against rDer p2 could be useful for the environmental studies and for the standardization of mite allergen extracts.

• 지역사회간호학회지
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• 제13권1호
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• pp.79-88
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• 2002

Purpose: The purpose of this study was to identify the indoor environment i.e. house type and age, ventilation method, use of bed & sofa, cockroach, ants, etc. on HDM (House dust mites), especially Der fI allergen. Method: Samples of dust from mattresses, pillows and the floor were collected by using a vacuum cleaner from April. 2000. The amount of Group I allergen(Der. fI) of HDM (house dust mites) were measured by two-site ELISA. Indoor Environmental characteristics were accessed by using questionnaires on 178 house wives living in the Seoul area. Results: The amount of HDM allergen (Der fI) was higher in sofa using house. There were higher concentrations of HDM allergen (Der fI) in sofas made from quilt material than for those that were made from leather. Homes that had pets like dogs living in them had higher concentrations of HDM allergen (Der fI) than without dogs. Conclusion: The photophobic mites thrive in dark. warm and humid environments; Items such as pillows. mattresses, box springs, blankets. carpets. and upholstered furniture should be considered ideal environments for the mite. Therefore, an indoor environmental control program should be carried out to avoid the HDM allergen.

• 한국보건간호학회지
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• 제12권2호
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• pp.136-148
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• 1998

This study was conducted to provide basic knowledge of effective environmental control of patients with allergic rhinitis during the period of July to December 1995. The subjects were consisted of 29 patients with allergic rhinitis. Each patient of nasal symptom scores assessed and dust samples drowned from their bedrooms twice for 1 month interval. The data. 58 samples were analyzed by using ANOVA. t-test and stepwise multiple regression. The results were as follows 1. Out of a total 52 dust samples $(89.7\%)$ were isolated house mites. Total 815mites/0.5gm dust were collected and identified the most prevalent house mites was Pyroglypidae mite with $81.1\%$ 2. The amount of house dust mites in the group of bedding laundry with hot water was significantly lower than that those of with cold water. 3. The nasal symptoms of the patients in the group of bedding laundry with hot water was significantly less than that those of with cold water. and also those of using large size bedroom was significantly lower than that those of using small size one. The nasal symptoms of the patients was closely associated with the washing water temperature. bedroom size. bedroom temperature. type of bedclothes, and frequency of cleanig room.

• Parasites, Hosts and Diseases
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• 제35권3호
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• pp.149-154
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• 1997

저장 곡류 해충으로 알려져 있는 긴털가루진드기(rophnwpkescentios)가 우리 나라 가옥 내 집먼지 서식 진드기류 중 제 3의 우점종으로 밝혀짐에 따라 알레르기성 질환을 야기하는 원인 항원으로 작용할 가능성이 높아져 연구의 대상이 되고 있다. 본 연구에서는 진단, 치료 및 면역학 적 연구에 필요한 다량의 항원을 공급하기 위하여 대량 사육방법의 개발을 시도하여 다음과 같은 결과를 얻었다. 긴털가루진드기를 대량 사육하는데 가장 적합한 사육배지로는 실험동물사료 분말 로 사육 10주 후 1,251.5배의 개체 증가를 보였고 효소분말과 실험동물 사료분말의 혼합배지 (1 : 1)에서도 사육 10주 후 1.203.1배의 개체 증가를 보여 거의 같은 성적을 보였다. 사육조건으 로 가장 적합한 온도와 상대습도는 $25^{\circ}C$. 64% RH로서 9주 후 960배로 가장 높은 증식결과를 보였고, $^25{\circ}C$와 75% RH에서도 935.4배의 개체 증가로 거의 동일한 결과를 나타냈다 사육용기 표면적을 $79{\;}cm^2,{\;}154{\;}cm^2{\;}및{\;}227{\;}cm^2$로 사육 비교한 결과 각각 860.8배. 924.6배 및 1,250.8 배의 개체 증가를 보여 현저한 차이는 보이지 않았다.

• 대한의생명과학회지
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• 제15권1호
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• pp.93-96
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• 2009

House dust mites (HDMs) play an important role in the occurrence of allergic diseases such as asthma and atopic dermatitis. Dermatophagoides pteronyssinus (Der p) is one of the most prevalent HDMs. It mediates the activation of T cells and monocytes, and induces the elevation of immunoglobulin E levels in allergic diseases. However, the effects of Der p on human monocytes have not been fully understood. In the present study, we investigated whether or not Der p has a great effect on the chemotactic activity of the human monocytic cell line, THP-1 cells, as induced by CC chemokines. We also show that the Der p extract (DpE) increased the chemotactic activity of THP-1 cells in response to MCP-1, RANTES, MIP-1${\alpha}$, and TARC, but had no effect on the expressions of CC chemokine receptors (CCRs) binding to CC chemokines in THP-1 cells. Protease inhibitors, such as aprotinin and E64, blocked the increased chemotaxis, while cytoplasmic $Ca^{2+}$ influx mediated by these chemokines was inhibited by DpE. These results indicate that DpE increases the chemotactic activity of THP-1 cells in response to CC chemokines by regulating the cells' protease-dependent mechanism. This finding may be useful in identifying the pathogenesis of allergic diseases induced by Der p.