• Journal of Preventive Medicine and Public Health
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• 제37권1호
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• pp.72-79
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• 2004

Objectives : This cross-sectional study aimed to quantify the relationship between the bone mineral density at the os calcis and the body mass composition in healthy children. Methods : The areal bone mineral density was measured at the os calcis with peripheral dual energy X-ray absorptiometry. The fat free mass, fat mass and percentage fat mass were measured using bioelectric impedance, in 237 Korean children, aged 9 to 12 years. The sexual maturity was determined by self assessment, using standardized series of the 5 Tanner stage drawings, accompanied by explanatory text. Results : From multiple linear regression models, adjusted for age, sexual maturity and height, the fat free mass was found to be the best predictor of the calcaneal bone mineral density in both sexes. About 15 and 20% variabilities were found in the calcaneal bone mineral densities of the boys and girls, respectively, which can be explained by the fat free mass. After weight adjustment, the percentage fat mass was negatively associated with the calcaneal bone mineral density in both sexes. Conclusions : The findings of this study suggest that the fat free mass, among the body compositions, is the major determinant of bone mineral density at the os calcis in Korean children aged 9 to 12 years. Obesity, defined as the percentage fat mass, is assumed to have a negative effect on the calcaneal bone density in children of the same weight.

• International Journal of Industrial Entomology and Biomaterials
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• 제2권1호
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• pp.19-26
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• 2001

Transformed Spodoptera frugiperda (Sf9) cells expressing baculovirus very late factor (VLF-1) were constructed by using Autograha nuclear polyhedrosis virus (AcNPV) immediate earthy gene (ie1). Neomycin-resistance gene as a selectable marker was introduced under the control of AcNPV ie1 promoter, and Bombyx mori nuclear polyhedrosis (BmNPV-K1) vlf-1 gene was introduced under the control of the Drosophila heat shock protein gene (hspr70) promoter to yield dual expression plasmid with two independent transcription units. It was transfected into Sf9 cells and cell clones expressing vlf-1 were selected by G4l8 treatment. Genomic DNA from transformed cells was isolated and integration of AcNPV iel harboring vlf-1 was confirmed by PCR using AcNPV iel-specific primers and Southern blot analysis. The transformed cells expressing VLF-1 in an infection-independent manner expressed foreign gene product of recombinant baculovirus in the earlier stage of infection compared with control Sf9 cells. These results suggest the possible to develop highly efficient transformed insect cells for baculovirus expression vector system.

• 한국정보통신학회논문지
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• 제14권9호
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• pp.2083-2090
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• 2010

본 논문에서는 고속 이동 컨테이너 화물을 효율적으로 검색하기 위한 스테레오 방사선 영상 기반 3차원 형상화 연구에 관하여 기술하였다. 스테레오 방사선 영상은 X-선 발생장치, 선형 방사선 센서 그리고 이동 스테이지로 구성한 일련의 장치로부터 실물을 대상으로 획득하였다. 두 개의 방사선 센서는 검색 대상체의 정류된 X-선 영상을 얻도록 설계 및 제작되었다. 스테레오 X-선 영상을 이용하여 두 영상간의 대응점을 찾는 매칭 알고리즘을 구현하고, 대상체의 실제 3차원 형상을 복원과정을 연구하였다. 대상체는 컨테이너 화물을 모사하기 위해 평형 철재 상자 내부에 넣은 다음 시험하였다. 3가지 대상체에 대해 스캔시험과 3차원 복원과정을 차례로 진행하였으며, 방사선 영상의 정보부족으로 인한 한계를 극복하기 위해 에지정보를 기반으로한 영상복원을 시도하였다. 시험결과는 제안한 기술이 컨테이너 화물 검색에서 보다 효율적인 정보를 제공할 수 있음을 확인시켜 주었다.

• International Journal of Industrial Entomology and Biomaterials
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• 제24권2호
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• pp.49-55
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• 2012

We previously isolated 9 clones that show stronger signal compared to B. mori cytoplasmic actin gene (BmA3) by using a dot blot hybridization. In this study, we focused on one clone among these clones which has high amino acid homology with elongation factor ${\alpha}$ gene of B. mori. This clone, named $bEF1{\alpha}$ (B. mori elongation factor ${\alpha}$) was ubiquitously expressed in all tissues and developmental stage of B. mori. As result of promoter assay using dual luciferase assay system, we found the highest transcription activity region (-702/+38) in the 5'-flanking region of $bEF1{\alpha}$ gene, which has about 20 fold more intensive promoter activity than BmA3 promoter. Moreover, the $bEF1{\alpha}$ promoter was normally regulated in Bm5, Sf9, and S2 cells. Therefore, we suggest that $bEF1{\alpha}$ promoter may be used more powerful and effectively for transgene expression in various insects containing B. mori as a universal promoter.

• Asian Pacific Journal of Cancer Prevention
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• 제15권21호
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• pp.9355-9360
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• 2014

Background: Oral squamous cell carcinoma (OSCC) remains as one of the most difficult malignancies to control because of its high propensity for local invasion and cervical lymph node dissemination. In this study, we evaluate the efficacy of our novel pH and temperature sensitive doxorubicin-methotrexate-loaded nanoparticles (DOX-MTX NP) in affecting HER2 expression profile in OSCC model in rat. Results: DOX-MTX- nanoparticle complexes caused significant decrease in mRNA level of HER2 compared to untreated cancers (p<0.05) and this finding was more pronounced with the IV mode (p<0.000). Surprisingly, HER2 mRNA was not affected in DOX treated as compared to the control group (p>0.05). On the other hand, in the DOX-MTX NP treated group, fewer tumors characterized with advanced stage and decreased HER2 paralleled improved clinical outcome (P<0.05). Moreover, the effectiveness of the oral route in the group treated with nanodrug accounted for the enhanced bioavailability of nanoparticulated DOX-MTX compared to free DOX. Furthermore, there was no significant difference in mRNA level of HER2 (p>0.05). Conclusions: Influence of HER2 gene expression is a new feature and mechanism of action observed only in dual action DOX-MTX-NPs treated groups. Down-regulation of HER2 mRNA as a promising marker and prognosticator of OSCC adds to the cytotoxic benefits of DOX in its new formulation. Both oral and IV application of this nanodrug could be used, with no preferences in term of their safety or toxicity. As HER2 is expressed abundantly by a wide spectrum of tumors, i DOX-MTX NPs may be useful for a wide-spectrum of lesions. However, molecular mechanisms underlying HER2 down regulation induced by DOX-MTX NPs remain to be addressed.

• 가정과삶의질연구
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• 제8권2호
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• pp.49-68
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• 1990

Family Life Education(FLE) is an educational specialty which was originated in reaction to changing social conditions, industrialization, and urbanization It deals with the perceived inadequacies of families to cope with these changes, thereby reducing social problems involved with and improving family life. With an assumption that the studies about FLE have not been so active in Korea, the present thesis examines the FLE in Korea; i.e. the definition, the objectives, the scope, the approximation of family life education, and the necessity of marital education in Korea based upon the published theories of FLE in th United Stated of America. Also it attempts to formulate a tentative plan for the promotion of a FLE program in Korea. The concept of FLE in Korea was manifested in the social educational law and life-span education, specified as the 4th among ten presidential regulations; and it has drawn an institutional, governmental attention It is defined as " the life-span developmental education to enhance the quality of human life, to solve family problem, to develop an individual's potentiality, and to strengthen the family correlations." of the FLE programs, the marital education is considered one of the crucial subjects because it is the core of the family life. With this premise, FLE tries to support the healthy marital relations, , subsequently helping to explore the family potentiality and to strengthen family ties, Considering the seemingly dual characteristics of the Korean family types, the contents of marital education program should be consisted of the following; marital communication, role cooperation, and effective expressions of mutual affection, In addition, reciprocal understanding and cooperation of the married couples to overcome the differences of value, personality, hobby, and religion, the educational methods in raising kids, and the effective management of home economics should be included. The objects of the FLE program are unmarried, pre-married, and married persons. Fro the married persons, the FLE program should be arranged in accordance with their marital status divided by the family life cycle so that they may prevent possible family problems at each stage of the family life. Also, to prevent the problems incurred in the curse of carrying out family functions, the FLE program should be provided with on the basis of a family unit, thereby improving the quality of the family functions.

• 전자공학회논문지C
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• 제34C권11호
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• pp.1-11
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• 1997

The transient current components of the dRAM are analyzed and the sensing current, data path operation current and DC leakage current are revealed to be the major curretn components. It is expected that the supply voltage of less than 1.5V with low VT MOS witll be used in multi-giga bit dRAM. A low voltage dual VT self-timed CMOS logic in which the subthreshold leakage current path is blocked by a large high-VT MOS is proposed. An active signal at each node of the nature speeds up the signal propagation and enables the synchronous DRAM to adopt a fast pipelining scheme. The sensing current can be reduced by adopting 8 bit prefetch scheme with 1.2V VDD. Although the total cycle time for the sequential 8 bit read is the same as that of the 3.3V conventional DRAM, the sensing current is loered to 0.7mA or less than 2.3% of the current of 3.3V conventional DRAM. 4 stage pipeline scheme is used to rduce the power consumption in the 4 giga bit DRAM data path of which length and RC delay amount to 3 cm and 23.3ns, respectively. A simple wave pipeline scheme is used in the data path where 4 sequential data pulses of 5 ns width are concurrently transferred. With the reduction of the supply voltage from 3.3V to 1.2V, the operation current is lowered from 22mA to 2.5mA while the operation speed is enhanced more than 4 times with 6 ns cycle time.

• 한국인터넷방송통신학회논문지
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• 제15권2호
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• pp.175-180
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• 2015

본 논문에서는 GPS/GLONASS 수신용 소형 액티브 안테나를 제안하였다. GPS(1.575.42MHz)와 GLONASS(1.602MHz) 듀얼 대역을 지원하는 마이크로스트립 패치 안테나를 최적화하였고, 안테나의 크기는 $13{\times}13{\times}3.6mm$이다. 제안한 안테나 특성 확인을 위하여 지그 크기 변화를 주었고, 패치안테나의 급전 간격을 조정하였고, LNA 쉴드 케이스 유무에 따른 변화로 확인하였다. 안테나 지그의 크기는 $65.6{\times}13{\times}0.8mm$이다. GPS 대역의 최대 이득은 3.78dBi이고, GLONASS 대역의 최대 이득은 4dBi이다. 위성 수신레벨을 증폭하기 위한 저잡음 증폭기는 1단 LNA를 설계하였다. LNA 칩은 BGA715N7를 이용하였고, LNA 이득은 19.9dB이다. 시뮬레이션과 측정 데이터를 비교 분석한 결과 GPS/GLONASS 수신용 소형 액티브 안테나의 실용화 가능성을 확인할 수 있었다.

• 대한전자공학회논문지TC
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• 제49권2호
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• pp.72-75
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• 2012

본 논문에서는 무선전력전송을 위한 고효율 DC-AC 변환 회로를 구현하였다. DC-AC 변환 회로는 발진기와 전력증폭기를 결합시켜 구현하였다. 전력증폭기의 전력 효율은 무선전력전송 송신 시스템의 효율에 크게 영향을 주기 때문에 Class-E 증폭구조를 이용하여 고효율 전력증폭기를 구현하였다. 또한, 전력증폭기의 입력 단에 연결되는 발진기의 출력 전력이 작기 때문에 높은 출력의 DC-AC 변환 회로를 구현하기 위하여 구동 증폭기를 이용한 고이득 이단 전력증폭기를 구현하였다. 고이득 고효율 이단 Class-E 전력증폭기의 입력 단에 발진기를 연결하여 고효율 DC-AC 변환 회로를 구현하였다. 13.56MHz의 2차, 3차 고조파 성분을 억제하기 위해 이중대역 저지 필터를 설계하여 결합하였다. DC-AC 변환 회로의 출력 전력과 변환 효율은 13.56 MHz에서 40 dBm과 80.2 %이다.

• Asian Pacific Journal of Cancer Prevention
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• 제15권5호
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• pp.1925-1929
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• 2014

Background: Vascular endothelial growth factor-C (VEGF-C), which contributes to lymphatic metastasis (LM) in malignant disease, is one of the most important factors involved in physical and pathological lymphangiogenesis. Some VEGF-C related factors such as sine oculis homeobox homolog (SIX) 1, contactin (CNTN) 1 and dual specificity phosphatase (DUSP) 6 have been extensively studied in malignancies, but their expression levels and associations have still to be elucidated in stomach cancer. Methods: We detected their expression levels in 30 paired stomach cancer tissues using quantitative real-time reverse transcription-PCR (qRT-PCR). The expression and clinical significance of each factor was analyzed using Wilcoxon signed rank sum test. The correlation among all the factors was performed by Spearman rank correlation analysis. Results: The results suggest that VEGF-C and CNTN1 are significantly correlated with tumor size, SIX1 with the age and CNTN1 also with the cTNM stage. There are significant correlations of expression levels among VEGF-C, SIX1, CNTN1 and DUSP6. Conclusions: There exists an important regulatory crosstalk involving SIX1, VEGF-C, CNTN1 and DUSP6 in stomach cancer.