• 한국지구과학회:학술대회논문집
• /
• 한국지구과학회 2005년도 춘계학술발표회 논문집
• /
• pp.188-188
• /
• 2005

• Archives of Pharmacal Research
• /
• 제23권2호
• /
• pp.121-127
• /
• 2000

Cannabigerol (1, CBG), methyl 4-[(2E)-3,7-dimethyl-2,6-octad ienyl)oxy]-3-methoxybenzoate (2, DTM), 5-fluorouracil (3, FU) as a reference, and cannabidiol (4, CBD) were tested for their growth inhibitory effects against KB(ATCC NO, OCL 17) cell lines using two different assays, the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazoliumbromide (MTT) assay and the sulforhod-amine B protein (SRB) assay. These compounds showed inhibitory activity in vitro in the micromolar range against KB cell lines. In general, the antitumor activities of these compounds (1, 2, 3 and 4) were dose-dependent over the micromolar concentration range of 1 to 100 M. The comparison of $IC_{50}$ values of these compounds in tumor cell lines showed that their susceptibility to these compounds decreases in the following order: DTM > CBD > 5-FU > CBG by MTT assay and DTM = CBD > 5-FU > CBG by SRB assay. CBG 1, DTM 2, 5-FU 3, and CBD 4 were tested for their cytotoxic effects on NIH 3T3 fibroblasts using two different assays, the MTT assay and SRB assay. These compounds exhibited potent cytotoxic activities in vitro in the micromolar range against NIH 3T3 fibroblasts. In general, the cytotoxic acivities of these compounds (1, 2, 3 and 4) were dose-dependent over the micromolar concentraion range of 1 to 100 M. The comparison of $CD_{50}$ values of these compounds in NIH 3T3 fibroblasts shows that their susceptibility to these compounds in decreases the following order(:) CBD > 5-FU > DTM > CBG by MTT assay, CBD > 5-FU > CBG > DTM by SRB assay. These results suggest that DTM 2 has the most growth-inhibitory activity against KB cell lines.

• Nutrition Research and Practice
• /
• 제9권2호
• /
• pp.129-136
• /
• 2015

BACKGROUND/OBJECTIVES: A variety of immunomodulators can improve the efficacy of low-dose chemotherapeutics. Active hexose correlated compound (AHCC), a mushroom mycelia extract, has been shown to be a strong immunomodulator. Whether AHCC could enhance the antitumor effect of low-dose 5-fluorouracil (5-FU) via regulation of host immunity is unknown. MATERIALS/METHODS: In the current study Hepatoma 22 (H22) tumor-bearing mice were treated with PBS, 5-FU ($10mg{\cdot}kg^{-1}{\cdot}d^{-1}$, i.p), or AHCC ($360mg{\cdot}kg^{-1}{\cdot}d^{-1}$, i.g) plus 5-FU, respectively, for 5 d. $CD^{3+}$, $CD^{4+}$, $CD^{8+}$, and NK in peripheral blood were detected by flow cytometry. ALT, AST, BUN, and Cr levels were measured by biochemical assay. IL-2 and $TNF{\alpha}$ in serum were measured using the RIA kit and apoptosis of tumor was detected by TUNEL staining. Bax, Bcl-2, and TS protein levels were measured by immunohistochemical staining and mRNA level was evaluated by RT-PCR. RESULTS: Diet consumption and body weight showed that AHCC had no apparent toxicity. AHCC could reverse liver injury and myelosuppression induced by 5-FU (P < 0.05). Compared to mice treated with 5-FU, mice treated with AHCC plus 5-FU had higher thymus index, percentages of $CD^{3+}$, $CD^{4+}$, and NK cells (P < 0.01), and ratio of $CD^{4+}$/$CD^{8+}$ (P < 0.01) in peripheral blood. Radioimmunoassay showed that mice treated with AHCC plus 5-FU had the highest serum levels of IL-2 and $TNF{\alpha}$ compared with the vehicle group and 5-FU group. More importantly, the combination of AHCC and 5-FU produced a more potent antitumor effect (P < 0.05) and caused more severe apoptosis in tumor tissue (P < 0.05) compared with the 5-FU group. In addition, the combination of AHCC and 5-FU further up-regulated the expression of Bcl-2 associated X protein (Bax) (P < 0.01), while it down-regulated the expression of B cell lymphoma 2 (Bcl-2) (P < 0.01). CONCLUSIONS: These results support the claim that AHCC might be beneficial for cancer patients receiving chemotherapy.

• Advances in Traditional Medicine
• /
• 제1권1호
• /
• pp.45-54
• /
• 2000

Cannabidiol derivatives (1, 2 and 3), and 5-fluorouracil (4, 5-FU) were tested for their growth inhibitory effects against human oral epitheloid carcinoma cell lines (KB) using two different 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and sulforhodamine B protein (SRB) assay. These compounds showed a potent inhibitory activity in vitro in the micromolar range against KB cell lines. In general, the antitumor activity of these compounds (1, 2, 3 and 4) was in a dose-dependent over the micromolar concentration ranges from $1\;{\mu}M\;to\;100\;{\mu}M$. The comparison of $IC_{50}$ values of these compounds in tumor cell lines shows that their susceptibility to these compounds decreases in the following order: CBD > 5-FU > CBDME > CBDDE by the MTT assay and SRB assay. Cannabidiol derivatives (1, 2 and 3), and 5-FU were tested for their cytotoxic effects on NIH 3T3 fibroblasts using two different MTT assay and SRB assay. These compounds exhibited potent cytotoxic activities in vitro in the micromolar range against NIH 3T3 fibroblasts. In general, the cytotoxic activities of these compounds (1, 2, 3 and 4) were in a dose-dependent over the micromolar concentration range $1\;{\mu}M\;to\;100\;{\mu}M$. The comparison of $CD_{50}$ values of these compounds on NIH 3T3 fibroblasts shows that their susceptibility to these compounds decreases in the following order; CBD > 5-FU > CBDDE > CBDME by MTT assay, CBD > 5-FU > CBDME > CBDDE by SRB assay. These results suggest that cannabidiol (1, CBD) retains the most growth-inhibitory activity against KB cell lines.

• 한국고분자학회:학술대회논문집
• /
• 한국고분자학회 2006년도 IUPAC International Symposium on Advanced Polymers for Emerging Technologies
• /
• pp.106-107
• /
• 2006

A series of vinyl monomers and their saturated model compounds containing different chromophores were synthesized. These monomers display strong intra-molecular fluorescence quenching, their fluorescence quantum yields and lifetimes are generally lower than those of their model compounds. It was found that the C=C bonds in these monomers played a key role in the intra-molecular quenching, which was confirmed by intermolecular fluorescence quenching and time-resolved fluorescence studies. On the basis of the intra-molecular quenching, a new fluorescence approach can be developed to monitor the process of the polymerization and curing of bismaleimides, which can directly reflect the C=C bond consumption during polymerization and curing.

• 동양고전연구
• /
• 제37호
• /
• pp.189-216
• /
• 2009

두보(杜甫)의 <음중팔선가(飮中八仙歌)>와 이를 화제(畵題)로 삼아 회화화(繪?化)한 시의도(詩意圖) 3편과, 원텍스트를 패러디한 <음중팔선도(飮中八仙圖)>를 다시 원텍스트로 삼아 패러디한 세편의 서발(序跋)을 살펴보면서 시라는 양식의 원텍스트가 그림이라는 양식으로, 다시 산문이라는 양식으로 그 모습을 바꾸면서 패러디되는 모습을 보았다. 개원(開元) 천보(天寶) 연간에 취중기행(醉中奇行)로 유명했던 여덟 명의 기인(奇人)을 노래한 두보(杜甫)의 <음중팔선가(飮中八仙歌)>는 많은 화가들에 의해 사랑받는 화제(畵題)가 되어 송(宋) 원(元) 명(明) 청(淸), 조선(朝鮮), 일본(日本) 등지에서 <음중팔선도(飮中八仙圖)>로 다시 태어났다. 이처럼 화가가시에 대한 해석을 회화적으로 재창조한 것을 시의도(詩意圖)라고 하는데 시의도(詩意圖)는 이미 존재하는 시를 전제로 하고 있으므로 이 둘 사이에는 원텍스트와 패러디 텍스트라는 관계가 성립한다. 두보의 마음에 공명한 화가들의 그림 3점, 명대(明代) 우구(尤求)의 <음중팔선도(飮中八仙圖)>, 조선(朝鮮) 김홍도(金弘道)의 <두보시의신선도(杜甫詩意神仙圖)>, 일본모모야마(桃山)시대의 가이호유쇼(海北友松)의 <음중팔선도(음중팔선도(飮中八仙圖)>를 살펴보며 원텍스트를 반복하면서도 각 화가의 개성에 따른 차이를 고찰하였다. 언어로 표현된 <음중팔선가(飮中八仙歌)>를 원텍스트로 삼아 패러디한 시의도(詩意圖) <음중팔선도(飮中八仙圖)>는 문인들이 그림에 부친 서문(序文) 및 제발문(題跋文)을 통해 다시 언어화된 텍스트로 패러디되었다. 화가의 마음에 공명한 조선의 세 문인 이덕무(李德懋), 박제가(朴齊家), 이봉환(李鳳煥)의 서발(序跋)을 통해 <음중팔선가(飮中八仙歌)>가 어떻게 변주되는지 살펴보았다. 이를 통해 원텍스트와 연속적인 관계에 의한 반복과 각 작가의 독자적인 해석에 의한 차이를 알 수 있었는데, 이덕무(李德懋)는 집정자에 대한 경계로, 이봉환은 회재불우(懷才不遇)한 현인(賢人)으로, 박제가는 현세의 규율을 초월한 천연(天然)스러운 선계(仙界)를 꿈꾸게 하는 대자유인으로 음중팔선(飮中八仙)을 이해하였음을 알 수 있었다.

• Asian Pacific Journal of Cancer Prevention
• /
• 제15권1호
• /
• pp.407-411
• /
• 2014

Objective: To observe local and systemic toxicity after sustained-release 5-fluorouracil (5-Fu) implantation in canine peritoneum and para-aortic abdominalis and the changes of drug concentration in the local implanted tissue with time. Methods: 300 mg sustained-release 5-Fu was implanted into canine peritoneum and para-aorta abdominalis. Samples were taken 3, 5, 7 and 10 days after implantation for assessment of changes and systemic reactions. High performance liquid chromatography was applied to detect the drug concentrations of peritoneal tissue at different distances from the implanted site, lymphatic tissue of para-aortic abdominalis, peripheral blood and portal venous blood. Results: 10 days after implantation, the drug concentrations in the peritoneum, lymphatic tissue and portal vein remained relatively high within 5 cm of the implanted site. There appeared inflammatory reaction in the local implanted tissue, but no visible pathological changes such as cell degeneration and necrosis, and systemic reaction like anorexia, nausea, vomiting and fever. Conclusions: Sustained-release 5-Fu implantation in canine peritoneum and para-aortic abdominalis can maintain a relatively high tumour-inhibiting concentration for a longer time in the local implanted area and portal vein, and has mild local and systemic reactions. Besides, it is safe and effective to prevent or treat recurrence of gastrointestinal tumours and liver metastasis.

• 한국수학교육학회지시리즈B:순수및응용수학
• /
• 제15권1호
• /
• pp.57-72
• /
• 2008

This paper is concerned with the numerical solutions to steady state nonlinear elliptical partial differential equations (PDE) of the form $u_{xx}+u_{yy}+Du_{x}+Eu_{y}+Fu=G$, where D, E, F are functions of x, y, u, $u_{x}$, and $u_{y}$, and G is a function of x and y. Dirichlet boundary conditions in a rectangular region are considered. We propose alternating direction shooting method for solving such nonlinear PDE. Numerical results show that the alternating direction shooting method performed better than the commonly used linearized iterative method.

• 셀메드
• /
• 제2권2호
• /
• pp.16.1-16.5
• /
• 2012

Fever in cancer patients is often due to the following causes: evil qi and toxity stagnancy, disorders of qi and blood, deficiencies of zang and fu organs, and the disorder of yin and yang. The treatments given to cancer patients with a fever are according to five: (a) Excessive inner heat and toxicants: remove heat and the toxicant, induce purgation. We use Cheng-Qi-Tang plus Qing-Wen-Bai-Du-Yin. (b) Tangle of damp and heat, and qi stagnancy: remove damp and heat, smooth the qi channel. We use Gan-Lu-Xiao-Du-Dan or San-Ren-Tang. (c) Obvious blood and heat stagnancy: remove heat and blood stasis. We use Xue-Fu- Zhu-Yu-Tang. (d) Deficiency of spleen qi, inner heat caused by a yin deficiency: nourish spleen qi and yin to remove the inner heat. We use Bu-Zhong-Yi-Qi-Tang or Xiao-Jian-Zhong-Tang. (e) Prominent yin deficiency and hectic fever: replenish yin and remove inner heat. We use Qing-Hao-Bie-Jia-Tang or Chai- Qian-Mei-Lian-San. The pathogenesis of fever in cancer patients is complicated. We can see both deficiency and excess in one differentiation. Therefore, we must make sure of it, then we can get the most effective treatment.

• Toxicological Research
• /
• 제16권2호
• /
• pp.101-107
• /
• 2000

Cannabidiol derivatives (1, 2 and 3), 5-fluorouracil (4, 5-FU) and adriamycin (5, AM) were tested for their growth inhibitory effects against human tumor cell lines using two different 3-{4,5-dimeth-ylthiazol-2-yl)-2,5-diphenyl-2H-tetrazolium bromide (MTT) assay and sulforhodamine B protein (SRB) assay. The light microscopic study showed morphological changes of the treated cells. Disruptions in cell organelles were determined by colorimetric methods; MTT assay and STB assay. These results suggest that cannabidiol (1, CBD) retains the most growth-inhibitory activity against human tumor cell lines.