• Journal of Microbiology and Biotechnology
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• v.33 no.9
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• pp.1228-1237
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• 2023

The CRISPR-Cas system has emerged as the most efficient genome editing technique for a wide range of cells. Delivery of the Cas9-sgRNA ribonucleoprotein complex (Cas9 RNP) has gained popularity. The objective of this study was to develop a quantitative polymerase chain reaction (qPCR)-based assay to quantify the double-strand break reaction mediated by Cas9 RNP. To accomplish this, the dextransucrase gene (dsr) from Leuconostoc citreum was selected as the target DNA. The Cas9 protein was produced using recombinant Escherichia coli BL21, and two sgRNAs were synthesized through in vitro transcription to facilitate binding with the dsr gene. Under optimized in vitro conditions, the 2.6 kb dsr DNA was specifically cleaved into 1.1 and 1.5 kb fragments by both Cas9-sgRNA365 and Cas9-sgRNA433. By monitoring changes in dsr concentration using qPCR, the endonuclease activities of the two Cas9 RNPs were measured, and their efficiencies were compared. Specifically, the specific activities of dsr365RNP and dsr433RNP were 28.74 and 34.48 (unit/㎍ RNP), respectively. The versatility of this method was also verified using different target genes, uracil phosphoribosyl transferase (upp) gene, of Bifidobacterium bifidum and specific sgRNAs. The assay method was also utilized to determine the impact of high electrical field on Cas9 RNP activity during an efficient electroporation process. Overall, the results demonstrated that the qPCR-based method is an effective tool for measuring the endonuclease activity of Cas9 RNP.

• Proceedings of the Korean Information Science Society Conference
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• 2004.10c
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• pp.286-288
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• 2004

소스 호스트와 목적지 호스트간 경유 경로를 패킷 헤더에 모두 포함하는 DSR(Dynamic Source Routing)은 경유 호스트 수 증가에 비례 칠서 패킷 헤더와 개별 호스트들이 유지하는 라우트 캐쉬의 크기가 커져야 한다. 따라서 경로 크기와 선형적 관계로 유발되는 확장성 문제로 DSR을 수천 개 이상의 호스트로 구성 되는 대규모 애드-혹 망에 적용하기 어렵다. IETF(Internet Engineering Task Force)는 이러한 단점의 회피 방법으로 Flow State 메카니즘을 제안 하고 있다. 그러나 이 방법은 Route Discovery를 위한 Route Request 와 Route Response 패킷에 전체 경로를 포함 해야 하는 것은 물론 Route Cache에도 전체 경로를 저장 해야 하기 때문에 DSR 확장성 문제를 해결 할 수 없다. 본 논문에서는 경로 크기에 비례해 증가하는 DSR의 오버헤드를 패킷 헤더는 6-hoP, 라우트 캐쉬는 12-hoP 크기로 한정 하는 경로 분할 라우팅 방법을 제안한다.

• Proceedings of the Korean Information Science Society Conference
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• 2003.10c
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• pp.469-471
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• 2003

본 논문에서는 이동 애드­혹 네트워크(mobile ad­hoc networks)의 대표적인 경로구축 방법인 DSR(dynamic source routing)을 확장한 R­DSR(robust DSR)을 제안한다. 제안된 R­DSR에서는 기존의 단일 경로 상의 2­흡 간격의 모든 노드들이 대체 노드를 경유하여 복수 개의 라우팅 경로를 유지하도록 구성되기 때문에, 경로 상의 일부 노드들이 이동하더라도 경로가 유지될 확률이 높아 메시지 전달률이 향상된다. 수학적 분석을 통하여 관련 연구 중 가장 우수한 것으로 알려진 Das의 다중경로 프로토콜보다 더 높은 라우팅 경로 유지 확률을 나타냄을 보였다. 아울러, 대체경로의 위상적 특성에 의해 빠른 대체경로로의 복구도 가능하다는 결론을 얻었다.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.12 no.6
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• pp.2785-2792
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• 2011

Such problems as delay, congestion, and crosstalk in the proxy system degrade not only QoS (Quality of Service) but responsiveness and reliability of the streaming media service. To solve this problem this paper proposed a FRTP (Fuzzy Relevance-based Transcoding Proxy) mechanism. The proposed FRTP mechanism analyzes fuzzy similarity for partitioned segment versions of media objects to create a FRTG (Fuzzy Relevance-based Transcoding Graph). Created FRTG determines the transcoding for partitioned media object segment versions. Determined transcoding improves DSR (Delay Saving Ratios), CHPR (Cache Hit Precision Ratio), and CHRR (Cache Hit Recall Ratio). The proposed mechanism is simulated to evaluate such performance parameters as DSR, CHPR, and CHRR. Simulation results shows that the proposed mechanism outperforms in DSR, CHPR and CHRR compared with the other existing mechanisms.

• 한국생물공학회:학술대회논문집
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• 2003.04a
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• pp.545-549
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• 2003

After irradiation of a cloned dextransucrase gene (dsrB742) with ultrasoft X-ray, an E. coli transformant (pDSRB742CK) was first developed for the expression of an extracellular dextransucrase, having increased activity and the synthesis of a highly branched dextran. Seven nucleotides of the parent gene (dsrB742) were changed in the nucleotide sequences of dsrB742ck. Among them, four nucleotides were changed at the ORF of dsrB742, resulting in a 30 amino acids deletion in the N-terminal of DSRB742 dextransucrase. The activity of DSRB742CK dextransucrase in culture supernatant was approximately 2.6 times higher (0.035 IU/ml) than that of the DSRB742 clone. The pDSRB742CK clone produced DSRB742CK dextransucrase when grown both on a sucrose medium (inducibly) and on a glucose medium (constitutively). The DSRB742 clone did not produce dextran constitutively on a glucose medium. DSRB742CK dextran had 15.6% branching and 2.7-times higher resistance to dextranase hydrolysis compared to DSRB742 dextran. $^{13}C-NMR$ showed that DSRB742CK dextran contained ${\alpha}-(1{\rightarrow}3)$ branch linkages that were not present in DSRB742 dextran.

• Journal of IKEEE
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• v.7 no.1 s.12
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• pp.63-71
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• 2003

In this paper, we propose an DSR(Dynamic Source Routing) protocol to support QoS for reliable data transmission in the mobile ad-hoc network. The proposed algorithm uses DSR protocol to support QoS as its basic routing protocol, and uses the nodes which are between source and destination nodes as key QoS support. Because of moving nodes there is some problem that is restricted reliable data transmission. For solve this problem, source node set up the QoS link with destination node. The nodes that are located at QoS link and find out loss of transmission path save the transmitting data packets. Those search a new transmission path to destination node and transmit the saved data packet to destination node. As the result of evaluation, we found the proposed QoS network guaranteed reliable data transmission with almost 100% data reception rate for slowly moving mobile ad-hoc network and with more 96% data reception rate, which is improvement of 3.7737% reception rate compared with none QoS network, for continuously fast moving mobile ad-hoc network.

• Textile Coloration and Finishing
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• v.8 no.1
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• pp.1-7
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• 1996

In discharge printing of cotton fabrics dyed with C.I. Reactive Orange 16(O-16), C.I. Reactive Blue 19(B-19) and C.I. Reactive Black 5(Bl-5), when the dyes were discharged by some chemicals, such as $K_{2}CO_{3}$, BASB, DSR, sarcosine and GSB, the single use of those chemicals made a very poor discharge, but mixing them with $K_{2}CO_{3}$ resulted to the outstanding improvement of discharge. Especially the dischargeability of $K_{2}CO_{3}$ BASB or $K_{2}CO_{3}$+DSR was very gratifying. For O―16, $K_{2}CO_{3}$+ DSR was slightly more effective than $K_{2}$CO$_{3}$+BASB, but for B―19 and Bl―5 $K_{2}CO_{3}$+BASB and $K_{2}CO_{3}$+DSR showed similar good results. In discharging of O―16 and B―19 by $K_{2}CO_{3}$+BASB or $K_{2}CO_{3}$ +DSR, the dischargeabilities of them increased as the time of 102$^{\circ}C$ steaming increased under the condition of 102$^{\circ}C$ steaming and no baking, but not under the condition of 102$^{\circ}C$ steaming and 16$0^{\circ}C$ baking. However for Bl-5, without regard to baking, the 102$^{\circ}C$ steaming of more than 15 minutes caused the discharge to be much more remarkable than that of 8 minutes did. Generally baking elevated the dischargeability, and this was sure in discharging by $K_{2}CO_{3}$+BASB or $K_{2}CO_{3}$+DSR. And it was confirmed that the structure of vinylsulfonyl reactive dyes could effect on the dischargeability because the three dyes, though little, showed different discharge behaviors.

• The KIPS Transactions:PartC
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• v.9C no.5
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• pp.647-654
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• 2002

Ad-hoc networks consist of a set of mobile hosts that communicate using wireless links, without the use of other supporting communication facilities (such as base stations, etc.). Therefore, the topology of an Ad-hoc network frequently changes due to the movement of mobile host, which nay lead to sudden packet loss. Recently, the large amount of research has focused on the routing protocols needed in such an environment. In this paper, TCP Reno, Sack, and Tahoe versions are analysed using DSR protocol which is the representative On-Demand routing protocol in Ad-hoc wireless network. As the result of this simulation, we know that TCP Reno relatively has higher throughput than that of Sack and Tahoe, and TCP Reno has more stable performance than that of TCP Tahoe and Sack, regardless of the speed of mobile node and the size of topology.

• Journal of the Korean Recycled Construction Resources Institute
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• v.9 no.3
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• pp.348-355
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• 2021

Asphalt, widely used for road pavement, has different required physical properties depending on the environment to which the road is exposed. Therefore, it is essential to maximize the life of asphalt roads by evaluating the physical properties of asphalt according to additives and selecting an appropriate formulation considering road traffic and climatic environment. Dynamic shear rheometer(DSR) test is mainly used to measure resistance to rutting among various physical properties of asphalt. However, the DSR test has limitations in that the results are different depending on the experimental setting and can only be measured within a specific temperature range. Therefore, in this study, to overcome the limitations of the DSR test, the rheological characteristics were predicted by learning the images collected from atomic force microscopy. Images and rheology properties were trained through EfficientNet, one of the deep learning architectures, and transfer learning was used to overcome the limitation of the deep learning model, which require many data. The trained model predicted the rheological properties of the asphalt binder with high accuracy even though different types of additives were used. In particular, it was possible to train faster than when transfer learning was not used.

• International Journal of Computer Science & Network Security
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• v.22 no.7
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• pp.13-20
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• 2022

Basically Mobile Ad Hoc Network (MANET) is an autonomous system with the collection of mobile nodes, these nodes are connected to each other by using wireless networks. A mobile ad hoc network poses this quality which makes topology in dynamic manner. As this type of network is Ad Hoc in nature hence it doesn't have fixed infrastructure. If a node wishes to transfer data from source node to a sink node in the network, the data must be passed through intermediate nodes to reach the destination node, hence in this process data packet loss occurs in various MANET protocols. This research study gives a comparison of various Mobile Ad Hoc Network routing protocols like proactive (DSDV) and reactive (AODV, DSR) by using random topology with more intermediate nodes using CBR traffic. Our simulation used 50, 100, and 150 nodes variations to examine the performance of the MANET routing protocols. We compared the performance of DSDV, AODV and DSR, MANET routing protocols with the result of existing protocol using NS-2 environment, on the basis of different performance parameters like Packet Delivery Ratio, average throughput and average end to end delay. Finally we found that our results are better in terms of throughput and packet delivery ratio along with low data loss.