• Journal of Animal Science and Technology
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• v.51 no.1
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• pp.25-32
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• 2009

This study was conducted to determine the effects of probiotics supplementation on growth performance, nutrient digestibility, occurrence of diarrhea and immune response in weaning pigs. Treatments were 1) NC (basal diet), 2) PC (basal diet + 0.12% avilamycin) and 3) A (basal diet + 0.2% Aspergillus oryzae), 4) B (basal diet + 0.2% Lactobacillus casei), 5) C (basal diet + 0.2% Bacillus subtilis), 6) D (basal diet + 0.2% Lactobacillus crispatus). A total of 120 pigs ($7.16\pm0.01$ kg average body weight, weaned at $23\pm3$days of age) were assigned to 6 treatments, 5 replicates and 4 pigs per pen in a randomized complete block (RCB) design. During the whole experimental period, body weight (P<0.01), average daily gain (ADG; P<0.01), and average daily feed intake (ADFI; P<0.05) of treatment PC were higher than other treatments. However, the probiotics treatments tended to increase ADG and G:F ratio compared to treatment NC. The G:F ratio in treatment A (Aspergillus oryzae) was similar to treatment PC during the whole experimental period (P<0.05). The supplementation of probiotics in the diet of weaning pig did not change nutrient digestibility (dry matter, crude protein, crude fat and ash) and nitrogen retention of weaning pigs. In blood urea nitrogen (BUN) concentration, treatment B had lower value than other treatments at 2 and 4 weeks (P<0.05). Treatments PC and C tended to decrease diarrhea score than other treatments (P=0.18). At 3h after lipopolysaccharide (LPS) injection, treatments NC and PC had higher count of $CD^{4+}$ T-cells than probiotics treatments, and treatment C showed the lowest value (P<0.01). There were no differences on count of $CD^{8+}$ T-cells and $CD^{4+}:CD^{8+}$ ratio among all treatments (P>0.10). These results suggest that the dietary probiotics are likely able to improve the growth performance, occurrence of diarrhea and immune response although they do not have similar effects like antibiotics in weaning pigs.

• Journal of The Korean Society of Grassland and Forage Science
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• v.30 no.1
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• pp.35-42
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• 2010

The study was conducted to investigate the effect of dietary energy and protein levels on milk production in Saanen (Capra hircus) dairy goats fed the diets with the different ratio of forage and concentrate. Four lactating goats in mid lactation ($57.5{\pm}10.8\;kg$ BW) were used in a $4{\times}4$ Latin square with 21-d periods. Animals were randomly allocated to one of the four groups; 10.59 MJ ME/kg and 11.90% CP (TJ), 10.14 MJ ME/kg and 12.73% CP (T2), 9.45 MJ ME/kg and 15.19% CP (T3), 8.75 MJ ME/kg and 16.60% CP DM (T4). Also ratio of forage to concentrate was 49: 51 (Tl), 59: 41 (T2), 74: 26 (T3) and 90: 10 (T4). Intakes of forage and concentrate were linearly increased (p<0.05) with their increasing energy levels in the diets. No significant differences were found in total dry matter intake and body weight between treatments. Milk yield in T3 was the highest among treatments (p<0.05). Relative to other treatments, T4 significantly (p

• Proceedings of the Korea Society of Poultry Science Conference
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• 2002.11a
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• pp.64-84
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• 2002

These studies were conducted to evaluate the Properties of lactic acid Producing bacteria(LAB), isolated from broiler and laying hens cecum and select the optimum strains to improve the performance, environment of poultry house, immunity, and intestinal microflora of broiler and laying hens. In experiment I , 23 LAB strains were isolated from broiler and laying hens cecum as a colony form. Six strains were selected by acid tolerance, bile salt tolerance, viability, enzyme release, antagonism, and antibiotics susceptibility. In Experiment II, selected LABs from Ex. 1 were conducted to investigate the effects of feeding various Lactobacillus on performance, nutrients digestibility, intestinal microflora, villi development and observation of epithelium surface, blood chemicals and fecal noxious gas of broiler chicks. One thousand eighty one day old broiler chicks were fed into Lactobacillus crispatus avibrol(LCB), Lactobacillus reuteri avibro2(LRB), Lactobacillus crispatus avihen1(LCH), and Lactobacillus vaginalis avihen2(LVH) at the level of 10$^4$ and 10$\^$7/cfu/g diet. Weight gam of chicks fed Lactobacillus tended to increase from the first week and was higher from 50 to 100g in Lactobacillus treatments than control. Feed intake and feed conversion were not statistically different of all treatments. Dry Matter digestibility of Lactobacillus treatments was prone to improve compared to that of control, but was not significantly different. Protein and Ca digestibility were also tended to improve in Lactobacillus treatments relative that of control. Lactobacillus treatments showed improved tendency in crude ash and fat compared to those of control, whereas phosphorus digestibility was not consistency. Nutrients digestibilities of bird fed LCH were superior to those of other treatments, It showed significantly higher in Ca and P digestibility than control(P〈0.05). Total Lactobacillus spp. of birds fed various Lactobacillus was significantly higher in illeum for five weeks(P〈0.05), but was not different at cecum. Yeast was thought to be not completely attached to intestinal lumen for one week. However, total number of yeast was significantly increased in cecum and illeum of three weeks old chicks (P〈0.05). The number of anaerobes exhibited to tendency the increase in Lactobacillus treatments from one week old of age at both ileum and cecum.

• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.10
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• pp.1606-1610
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• 2004

This study was. conducted to investigate the effects of $\delta$-aminolevulinic acid supplementation on growth. performance, nutrient digestibility and hematological changes in weaned pigs. Seventy five Duroc${\times}$Yorkshire${\times}$ Landrace pigs (7.21$\pm$0.02 kg average BW) were used in a 20 d growth assay. Dietary treatments included 1) NC (negative control; basal diet), 2) PC (positive control; NC diet + 0.1% Apramycin + 0.1% Oxytetracycline), 3) ALA0.l (NC diet+0.l% $\delta$-aminolevulinic acid), 4) ALA0.2 (NC diet+0.2% $\delta$-aminolevulinic acid) and 5) ALA + AB (PC diet+0.2% $\delta$-aminolevulinic acid). In entire experimental period, ADG (Average Daily Gain) was greater in pigs fed ALA + AB diet than in pigs fed NC diet (p<0.05). However, there was no significant difference in ADG for pigs fed PC, ALA0.l or ALA0.2 diets. Also, ADFI (Average Daily Feed Intake) and G/F (Gain/Feed) were not affected by treatments. For 20 d, fecal digestibilities of DM (Dry Matter) and N (Nitrogen) were higher (p<0.05) for pigs fed ALA + AB diet than for pigs fed NC and ALA0.l diets. Total protein concentration of serum was increased in ALA + AB treatment compared to NC and PC treatments (p<0.05). In iron concentration of serum, pigs fed $\delta$-aminolevulinic acid supplementation diets were greater than for pigs fed NC and PC diets (p<0.05). TIBC (Total Iron Binding Capacity) concentration of serum was increased in ALA + AB treatment compared to NC, PC and ALA0.l treatments (p<0.05). Pigs fed ALA + AB and ALA0.2 diets were higher hemoglobin (Hb) and hematocrit (HCT) concentration of blood than pigs fed NC and PC diets (p<0.05). RBC and WEC concentrations of blood were significantly higher (p<0.05) for the pigs fed ALA0.2 and ALA + AB diets than for pigs fed NC diet. Lymphocyte concentration of blood was increased in $\delta$-aminolevulinic acid supplementation treatment compared to NC treatment (p<0.05). In conclusion, $\delta$-aminolevulinic acid supplementation for weanling pigs had improved growth performance and nutrient digestibilities and had increased total protein, iron, hemoglobin and lymphocyte concentration of blood. Also, feeding for dietary $\delta$-aminolevulinic acid and antibiotic mixture supplementation may have more higher effective growth performance in weanling pigs.

• Journal of Animal Science and Technology
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• v.45 no.4
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• pp.529-536
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• 2003

A total of 72 finishing pigs(L${\times}$Y${\times}$D; 80kg of initial body weight) were employed for 5 weeks to investigate the effects of feeding mushroom substrate waste(MSW) treated with pleurotus ostreatus and probiotics on productivity, carcass traits, nutrient digestibility and emissions of harmful gases and malodor in manure. Treatments were Control(C: basal diet), T1(3% MSW) and T2(3% MSW+ 0.1% probiotics). Average daily gain(ADG) was lower(p<0.05) in pigs fed a T1 diet than those fed a C diet, however, there was no difference in ADG of pigs fed diets between C and T2. Similar trends were found in feed/gain(F/G) among treatments, though feed intake was not different. No differences were found in back fat thickness among treatments, but carcass dressing percentage was significantly(p<0.05) improved in pigs fed a T2 diet compared to C or T1 diets. Nutrient digestibilities including dry matter, crude protein, energy and crude fiber were lower(p<0.05) in T1 than C or T2. $NH_3$ and $H_2S$ gas emissions were reduced(p<0.05) or not produced in pig manure from T2 as compared to C or T1. In conclusion, the present result shows that feeding MSW with probiotics is desirable for finishing pigs in terms of productivity, carcass traits and nutrient digestibility. It also appears that the emission of harmful gases and malodor in manure can be reduced by the inclusion of probiotics in the diet.

• Journal of Animal Science and Technology
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• v.50 no.1
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• pp.79-88
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• 2008

This study was conducted to evaluate effects of dietary Escherichia coli phytase supplementation on growth performance, carcass quality and excretion of copper and zinc concentrations in finishing pigs. The total of seventy two [(Landrace×Yorkshire)×Duroc] pigs(65.43±0.72 kg in average initial body weight) were used in 56 days assay. Dietary treatments included 1) CON (basal diet) 2) P5(basal diet+phytase 0.05%) and 3) P10 (basal diet + phytase 0.1%). There were three dietary treatments with six replicate pens per treatment and four pigs per pen. During the overall periods, ADFI(Average daily feed intake) was increased (P<0.05) in phytase supplementation treatments compared to CON treatment. At the 5th week, dry matter, nitrogen and ash digestibilities were higher in P5 treatment than in CON and P10 treatments(P<0.05) and CON treatment showed the lowest effect on nutrition digestibility(P<0.05). However, phosphorus digestibility was higher in both phytase supplementation treatments than in CON treatment(P<0.05). The pH of M. logissimus dorsi was significantly higher in CON treatment than phytase supplementation treatments(P<0.05). L* value of M. logissimus dorsi muscle color was significantly increased(P<0.05) in P10 treatment compared to CON and P5 treatments. Also, a* value was increased (P<0.05) in CON treatment compared with phytase supplementation treatments. However, excretion of copper and zinc concentrations were no significant difference among the treatments. In conclusion, the effect of Escherichia coli phytase showed in ADFI, digestibilities, pH and color of meat in finishing pigs.

• Journal of Animal Science and Technology
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• v.55 no.1
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• pp.51-59
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• 2013

This study was conducted to investigate the dietary effect of total mixed rations (TMR) containing brewers grain, soybean curd and rice straw, respectively, on growth performance and carcass characteristics of Hanwoo steers. Twenty four Hanwoo steers (average body weight 168 kg) in 6 months age were randomly allocated to 4 experimental groups, which contained 6 animals per group during 22-months feeding trial. The treatment was composed of the group fed concentrate and rice straw separately (T1), the group fed TMR containing brewers grain (T2), the group fed TMR containing soybean curd (T3) and the group fed TMR containing rice straw (T4). Body weight (BW) was not different among treatment groups during the growing and middle fattening period. However, it was significantly greater in T4 group than in T2 group on the final BW (p<0.05). Average daily gain was significantly greater in TMR groups compared to T1 group on the growing and late fattening (p<0.05). However, it was not different in total average. Dry matter intake tended to be increased in TMR groups. The feed conversion ratio was significantly lower in T4 group compare to T2 group (p<0.05). According to the yield traits, carcass weight of T1, T2, T3 and T4 were 389.0, 368.4, 387.5 and 384.3 kg, respectively. The back fat thickness, rib-eye area, and yield index were not different among the groups. Meat color, fat color, texture and maturity were not significantly different among groups. However, marbling score and appearance rate of meat quality grade of over $1^+$ were greater in T2 and T3 groups compare to other groups. Therefore, these results indicated that TMR with brewers grain and soybean curd may be ameliorated the growth performance and carcass characteristics of Hanwoo steers.

• Asian-Australasian Journal of Animal Sciences
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• v.28 no.8
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• pp.1150-1154
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• 2015

This experiment was conducted to investigate the effects of palm kernel expellers on productive performance, nutrient digestibility, and changes in white blood cells (WBC) of lactating sows. A total of 14 sows ($200{\pm}12kg$ of average body weight [BW]; 2.5 of average parity) were used and moved from gestation room to farrowing room on d 109 of gestation. Sows were randomly assigned to 2 dietary treatments in a completely randomized design. The treatments were a diet based on corn and soybean meal (CON) and CON added with 20% of palm kernel expellers (PKE). Sows were fed the treatments for 28 days (weaning) after farrowing. Blood was collected from each sow and 4 randomly selected piglets from each sow before farrowing or on d 3, 7, or 14 of lactation. Sows were fed respective treatments containing 0.2% chromic oxide from d 15 to 21 of lactation. Fecal samples were collected daily for the last 3 days after the 4-d adjustment period. Measurements were performances and WBC changes of sows and litter, nutrient digestibility of sows, and daily diarrhea of litter. Sows fed PKE had greater average daily feed intake (7.38 vs 7.10 kg/d; p<0.05) and lost less BW (-6.85 vs -8.54 kg; p<0.05) and backfat depth (-0.42 vs -0.71 mm; p<0.05) than those fed CON. However, there were no differences on digestibility of dry matter, nitrogen, and energy and weaning to estrus interval of sows fed either CON or PKE. Piglets from sows fed PKE gained more BW (203 vs 181 g/d; p = 0.08) and had less frequency of diarrhea (6.80 vs 8.56%; p = 0.07) than those from sows fed CON. On the other hand, no difference was found on preweaning mortality of piglets from sows fed either CON or PKE. Sows fed PKE had lower number of WBC (9.57 vs $11.82{\times}10^3/{\mu}L$; p = 0.09) before farrowing than those fed CON, but no difference on d 3 and 7. Similarly, piglets from sows fed PKE had also lower number of WBC (7.86 vs $9.80{\times}10^3/{\mu}L$; p<0.05) on d 14 of lactation than those from sows fed CON, but no difference on d 3 and 7. In conclusion, addition of 20% palm kernel expellers to lactation diet based on corn and soybean meal had no negative effects on productive performance, nutrient digestibility, and WBC changes of lactating sows.

• Journal of Animal Science and Technology
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• v.48 no.4
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• pp.555-562
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• 2006

The objective of this study was to evaluate the effects of dietary cation-anion difference (DCAD) with or without ruminally protected fat and niacin on the thermoregulatory ability, milk yield and milk composition of lactating dairy cows during summer in Korea. Thirty mid-lactating Holstein cows (134±12.4 DIM and 23.4±2.3kg/d of milk yield) were divided into three groups (10 animals/group). Cows were housed in a free-stall barn and were provided with forced- air ventilation (wind velocity = 4 m/s) using 41 cm diameter fans. Diet one was formulated to contain low DCAD (+15 DCAD) while the remaining two diets were higher in DCAD (+30 DCAD). One higher DCAD diet was formulated to contain by-pass fat and the second higher DCAD diet contained the niacin along with by-pass fat. The maximum ambient temperature during July was 28.5℃ which could be seen as a period of mild heat stress. As summer progressed, August was characterized as a severe heat stress condition with maximum ambient temperature (32.4℃) and THI (74.0). Dry matter, crude protein and total digestible nutrients intake was not affected by the DCAD level and supplementation of ruminally protected fat or niacin. Milk production was higher in cows fed diets supplemented with fat and niacin than those fed un-supplemented diet. No difference in milk yield was observed in cows fed diets supplemented with fat or niacin plus fat. Milk fat and rectal temperature were not affected by the DCAD level and supplementation of ruminally protected fat or niacin. However, respiration rate was decreased in cows fed diets supplemented with either fat or fat and niacin compared to those fed. The results of the present study indicated that higher DCAD (+30) and supplementation of fat along with niacin can somehow mitigate the negative effects of heat stress on milk yield and physiology of lactating Holsteins during July and August in Korea. In present study reduced respiration rate and increased milk yield in lactating cows may be attributed to the cooling effect of supplemented fat along with vasodilatory functions of niacin. (Key Words: DCAD, Heat stress, THI, milk yield, Milk fat, Holstein)

• Journal of the Korea Academia-Industrial cooperation Society
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• v.21 no.5
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• pp.266-273
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• 2020

This study was undertaken to determine the effects of diet supplementation with complex probiotics (CPB), on growth performance, nutrient digestibility, blood metabolites, noxious gas, and fecal microflora in weaning pigs. On the basis of body weight, a total of 234 weaned pigs (Landrace×Yorkshire×Duroc, 6.14±0.78kg) were randomly allotted to 3 treatments and 6 replicates (13 pigs per pen). The experimental diets were fed in a meal form for 28 days (days 0-14, PhaseI, and days 15-28, PhaseII). The dietary treatment groups were as follows: T1 (basal diet), T2 (T1+0.13% CPB) and T3 (T1+0.25% CPB). The CPB supplement contained Bacillus subtilis 1.0×10⁶ CFU/g, Enterococcus faecium 1.0×10⁶ CFU/g, Saccharomyces cerevisiae 1.0×10⁶ CFU/g, Bacillus licheniformis 3.0×10⁸ CFU/g, and Bacillus polyfermenticus 3.0×10⁸ CFU/g. Pigs fed the T3 diet showed an increase (p<0.05) in the overall average daily gain and average daily feed intake, increased (p<0.05) crude protein digestibility in PhaseI, and greater (p<0.05) dry matter and gross energy digestibility in PhaseII. Supplementation of CPB had no effect on the blood profile. Furthermore, pigs fed the T3 diet had lower (p<0.05) NH₃ emission and overall count of fecal Clostridium spp. In conclusion, we believe that CPB supplementation has a beneficial effect on growth performance, nutrient digestibility, noxious gas, and fecal microflora in weaning pigs.