• 한국응용약물학회:학술대회논문집
• /
• 한국응용약물학회 1992년도 제1회 신약개발 연구발표회 초록집
• /
• pp.42-42
• /
• 1992

ras oncogene은 암조직이나 transformed human cell line에서 거의 공통적으로 발견되는 oncogene으로서 그 product인 p2l 단백질은 C-terminal 25개의 아미노산 외에는 거의 동일한 배열을 가지고 있는 매우 conservative한 단백질이며 C-terminal cysteine이 carboxy methylation되어 있고 또한 palmitic acid와 같은 long chain fatty acid도 결합되어 있다. 보고된 바에 의하면 p21 protein의 palmitation은 ras protein의 세포막에 대한 친화력을 유지시키며 이와 같은 친화력은 cell transforming activity의 기본요건으로 알려져 있다. 이와 같은 관점에서 볼때 p21 단백질의 C-terminal processing현상을 new drug target으로, 즉 p2l 단백질의 C-terminal processing을 억제하므로서 cell transforming activity를 저해 할 수 있을 것이므로 생체내에 존재하는 p21 단백질 C-terminal processing 억제제의 identification 및 purification은 항암제 연구와 밀접한 관계가 있다. 구체적으로 farnesyl-protein transferase inhibitor 혹은 carboxyl methyl inhibitor의 identification 및 purification은 이같은 목적을 달성 할 수 있는 가능성이 크다.

• 혜화의학회지
• /
• 제4권2호
• /
• pp.309-329
• /
• 1996

There are the Seven Effect of Drugs and unique processing methods in Chinese traditional medicine. The Seven Effects are single effrect(單行), additive effect(相加, 相須), synergic effect(上乘, 相使), antagonistic effect(相畏, 拮抗), inhibitory effect(相惡), neutralizing effect(相殺) and opposite effect(相反). We are interested in synergic effects of some drugs and components ; Addition of OLDENLANDIA DIFFUSA to Kilkyungtang combanation enhanced the cytotoxic activity of Kilkyungtang against A549 and B16-Fo eells by 20% and 50%, respectively. The Oldenlandia-added kilkyungtang also potentiated the cytotoxicities of mitomycine Cand 5-fluorouracil. ar-tunnerone. isolated from the root of Curcuma longa, potentiated the cytotoxic activity of sesquiphellandrene(isolated from the same root), aurapten(isolated from Aurantii semen)or cyclophosphamide by 10 times. The purpose of the processing(修治) of Chinese grugs is to remove unusable parts of plants and to eliminate toxicities as well as to produse new active components in drugs. On a occasion of study on the anthelmintic drugs against the chinese fluke(Clonorchis sinesis, (肝디스토마), we have observed that the processed mume fruit(鳥梅) possessed a very very potent clonorchicidal effect, while the methanol extract of the non-processed fruit inactive. The active component was isolated from the processed mume and identified as 5-hydroxymethylfurfuryl aldehyde. This substance dose not occur in the immature fruit and was found only in the processed one. Wehave heated the immature fruit in an oven at $90^{\circ}C$ for 52 hrs and found that the heated fruit eame clonorchidal. As demonstrated in these and other example cited in this presentation, the natural products chemistry is contributory to univeiling the drug effect ensued from the processing and the synergic effect of Oriental medical drug combinations, and to rationalization or modernization of the traditional medicine.

• 한국식품위생안전성학회지
• /
• 제25권4호
• /
• pp.281-288
• /
• 2010

식품 중 아플라톡신 ($B_1+B_2+G_1+G_2$)의 기준은 곡류, 두류, 땅콩, 견과류 및 그 단순가공품 (분쇄, 절단 등), 곡류 가공품 및 두류가공품, 과자류 (땅콩 또는 견과류 함유식품), 장류, 고춧가루, 팝콘용 옥수수가공품, 기타 식품류 (찐쌀)에 대하여 15 ($B_1$은 10)${\mu}g/kg$ 이하로, 원유 및 우유류 중 아플라톡신의 기준은 $M_1$으로서 0.5 ${\mu}g/kg$ 이하로 설정되었다. 사과주스, 사과주스 농축액 중 파튤린의 기준은 50 ${\mu}g/kg$ 이하로 설정되어 있으며, 오크라톡신 A의 기준은 밀, 호밀, 보리, 커피콩, 북은 커피에 5 ${\mu}g/kg$, 인스턴트커피, 건포도 중 10 ${\mu}g/kg$, 포도주스, 포도주스 농축액, 포도주 중 2 ${\mu}g/kg$ 이하로 설정되었고, 푸모니신($B_1+B_2$)은 옥수수 중 4 mg/kg, 옥수수 단순가공품 및 옥수수 가루 중 2 mg/kg, 옥수수 가공품 중 1 mg/kg 이하로 설정되었다. 현재 식품 중 곰팡이독소에 대한 안전관리는 노출량 조사와 위해평가를 통해 합리적이고 과학적으로 설정되어 관리하고 있다.

• 생약학회지
• /
• 제27권3호
• /
• pp.196-206
• /
• 1996

In order to characterize the chemical change, of the constituents and pharmacological transformation of Glycyrrhizae Radix(GR) which may occur during its processing, processed GR has been investigated in comparison with unprocessed GR. It was found that glycyrrhizin(GL) and other several constituents contained in GR were remarkably changed by processing, and the decrease of contents of GL was identified by making use of thin layer chromatography-chromatoscanner. And also, it was found that glycyrrhetic acid (GA) was identified from processed GR. We recognized that commonly processed GR showed less hemolytic effect than unprocessed GR and processed GR at $190{\circ}c$. It was also found that commonly processed GR showed more effective analgesic effect and prolonged the duration of hypnosis induced by pentobarbital-Na in mice as compared with corresponding unprocessed GR and processed GR at $190{\circ}c$. And then, commonly processed GR significantly suppressed the increases of s-GOT, s-GPT and s-LDH activities in injured mice induced by $CCl_4$.

• 분석과학
• /
• 제18권5호
• /
• pp.403-409
• /
• 2005

최근 내분비계장애물질로 알려진 PAHs 화합물 중 하나인 벤조피렌은 대표적인 PAHs 화합물로 음식을 조리, 가공할 때 식품의 주성분인 탄수화물, 단백질, 지방 등이 분해되어 생성되는 열분해 산물이며 토양, 공기, 물, 식품 등 전 환경매체에서 검출되고 있다. 본 연구에서는 PAHs[benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, benzo(g,h,i)perylene, indeno(1,2,3-c,d)pyrene]에 대하여 어류 중 지방의 알칼리 분해시간에 따른 분해효율, 추출 용매에 따른 추출효율, 정제컬럼의 용출량에 따른 정제효율 등을 비교 실험하여 알칼리분해를 이용한 어류 중 PAHs의 전처리방법을 확립하고자 하였다. 균질화된 시료를 알칼리 분해하여 n-hexane으로 추출하고 증류수로 세척한 후 Sep-Pak florisil cartridge로 정제하여 HPLC/FLD(고속액체크로마토그래피/형광검출기) 로 동시 정량 분석하였으며 각각의 PAHs에 대한 회수율은 약 90~106% 수준이었다.

• Archives of Pharmacal Research
• /
• 제26권11호
• /
• pp.929-936
• /
• 2003

Methanol extract (MeOH), n-hexane (Hx), chloroform ($CHCl_3$), ethyl acetate (EA), butanol (BuOH) and aqueous ($H_2O$) fractions of Eucommiae Cortex including geniposidic acid (GA), geniposide (GP) and aucubin (AU) were tested for their therapeutic efficacy on osteoporosis. The contents of GA, GP and AU in the cortex and leaf of Eucommia ulmoides Oliver were quantified by HPLC. The effect of Eucommiae Cortex on the induction of growth hormone (GH) release was studied by using rat pituitary cells. The proliferation of osteoblast-like cells increased by herbal extracts was assayed using a tetrazolium (MTT), alkaline phosphatase (ALP) activity, and [$^3H$]-proline incorporation assays. The inhibition of osteoclast was studied by using the coculture of mouse bone marrow cells and ST-2 cells. As a result, the GA, GP and AU were present in the cortex more than in the leaf of E. ulmoides Oliver. The MeOH (1mg/mL), Hx, $CHCl_3$ and EA fractions (each 20 $\mu$ g/mL) had potent induction of GH release. The $CHCl_3$ exhibited the potent proliferation of osteoblasts. The AU, GP and GA were increased proliferation of osteoblasts. In addition, GA ($IC_{50}: 4.43{\times}10^{-7}$M), AU and GP were significantly inhibited proliferation of osteoclast. In summary, it is thought that the components in a part of the fractions of Eucommiae Cortex participate in each step of mechanism for activating osteoblast to facilitate osteogenesis, and suppress osteoclast activity to inhibit osteolysis.

• 생약학회지
• /
• 제36권2호통권141호
• /
• pp.75-80
• /
• 2005

This study is concerning to identify the hygienic problems occurring in processing, storage, and distribution of Rehmanniae radix crude, and to investigate the possibility of application of safe and hygienic ${\gamma}-irradiation$ techniques. The results are as follows. To compare the contents of catapol, index compound of Rehmanniae Radix crude, between before and after ${\gamma}-irradiated$,0,2.5,5,7.5,10,12.5,15,20, and 30 kGy of ${\gamma}-irradiation$ was applied to standard catapol and Rehmanniae radix crude. The contents of catapol in standard material and Rehmanniae radix crude were decreased with the increase of ${\gamma}-irradiation$ level. However, active components of Rehmanniae Radix crude were not changed with ${\gamma}-irradiation$. These results indicate that active components of Rehmanniae Radix crude were preserved after ${\gamma}-irradiation$ and those of Rehmanniae Radix crude did not showed significant change after irradiation of ${\gamma}-ray$ up to 20 kGy.

• 한국축산식품학회지
• /
• 제31권1호
• /
• pp.40-46
• /
• 2011

The growth profile of Bacillus cereus in ready-to-eat (RTE) food products of animal origin was examined under different temperature and incubation conditions. In sandwiches and Kimbab, B. cereus did not grow or exhibited only minimal growth at 4 and $10^{\circ}C$, but it grew rapidly at ambient temperature. In sandwiches, B. cereus did not grow efficiently at $25^{\circ}C$, however, in ham, the main ingredient of sandwiches, B. cereus growth was observed at the same temperature, with bacterial levels reaching 7.94 Log CFU/g after incubation for 24 h at $25^{\circ}C$. Toxigenicity of B. cereus was observed only at temperatures above $25^{\circ}C$. In Kimbab, B. cereus produced toxin after 9 h at $30^{\circ}C$ and after 12 h at $25^{\circ}C$. Ingredients of sandwiches and Kimbab were collected from 3 different Korean food-processing companies to investigate the source of contamination by B. cereus. Among the 13 tested food items, 6 items including ham were found to be contaminated with B. cereus. Of these ingredients, B. cereus isolates from 3 items produced enterotoxins. None of these isolates harbored the emetic toxin-producing gene. The findings of the present study can be used for risk assessments of food products, including ham and cheese, contaminated with B. cereus.

• 대한예방한의학회지
• /
• 제4권2호
• /
• pp.258-272
• /
• 2000

The cytotoxic activities of Pinellia ternate, Aconitum carmichaeli, Arisaema amurense, Aconitum kusnezoffii and Scutellaria baicalensis on monkey kidney cell (Vero) and human liver cell (WRL68) were evaluated by Sulforhodamine B Protein (SRB) and Tetrazolium-based (MTT) colorimetric assay methods The results were as fellows : 1 The Pinellia ternate and Arisaema amurense did not show the cytotoxic activities at any concentration without processing or natural drugs. 2 The extracts of Aconitum carmichaeli, Aconitum kusnezoffii and Scutellaria baicalensis showed cytotoxic activities. However, the cytotoxic activities of processing drugs were less effective than the natural drugs. 3. The cytotoxic activities on monkey kidney cell (Vero) and human liver cell (WRL68) of Aconitum carmichaeli was determined by MTT assay. The Kyungpo(京?) of Aconitum carmichaeli showed less concentrate than that of the Dangpo(唐?). The $IC_{50}$ value on monkey kidney cell (Vero) and human liver cell (WRL68) of Kyungpo was $937{\pm}29\;and\;731{\pm}31{\mu}g/ml$, respectively 4. The cytotoxicity on monkey kidney cell (Vero) and human liver cell (WRL68) of Scutellaria baicalensis showed strong activities without processing or natural drugs.

• 한국식품과학회지
• /
• 제45권5호
• /
• pp.545-549
• /
• 2013

본 연구에서는 GC-FID를 이용하여 식품첨가물공전에 사용량이 정해져 있는 빵류, 캡슐류, 건조과실류 채소류 및 과실류 채소류 중 유동파라핀의 함량을 측정하였다. 시료에서 n-hexane으로 유동파라핀을 추출하고 $KMnO_4$ 산화반응을 통해 추출물 중 불포화탄화수소의 극성을 증대시킨 다음 aluminium oxide를 충진한 SPE 카트리지로 정제하여 GC-FID로 측정하였다. 검출한계와 정량한계는 각각 10 mg/kg과 20 mg/kg으로 개별 n-alkane으로 정량하는 방법이 아닌 넓은 봉우리로 나타나는 유동파라핀의 면적의 합으로 정량하는 문헌들(15,17)의 값과 유사하게 나타났다. 유동파라핀은 빵류, 건조과실류 채소류 및 캡슐류에서 이형제 등으로 사용되고 있으나 최종 완제품에서 사용기준 이하로 검출되었고, 과실류 채소류에서 모두 불검출로 나타남에 따라 안전한 수준으로 관리되고 있음을 알 수 있었다.