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Pretreatment of Fish for the determination of polycyclic aromatic hydrocarbons using alkali digestion  

Hu, Soojung (Food & Drug Exposure Assessment Division, National Institute of Toxicological Research, Korea Food & Drug Administration)
Lee, Hyomin (Food & Drug Exposure Assessment Division, National Institute of Toxicological Research, Korea Food & Drug Administration)
Chae, Youngzoo (Seoul Metropolitan Government Research Institute of Public Health & Environment Gangnam Agrocultural Products Inspection Team)
Yoo, Eun-Ah (Department of Chemistry, Sungshin Women's University)
Publication Information
Analytical Science and Technology / v.18, no.5, 2005 , pp. 403-409 More about this Journal
Abstract
Polycyclic Aromatic Hydrocarbons(PAHs) contamination arises from several sources including processing of food(smoking, direct drying, cooking) and environmental contamination of air, water, or soil, the later being considered as the most important. In this study, to establish the analytical method for some PAHs[benzo(a)anthracene, chrysene, benzo(b)fluoranthene, benzo(k)fluoranthene, benzo(a)pyrene, dibenzo(a,h)anthracene, benzo(g,h,i)perylene, indeno(1,2,3-c,d)pyrene] in fish, alkali digestion time, extraction solvents, elution volume of florisil cartridge for clean-up have been optimized. The methodology involved saponification and extraction with n-hexane, clean-up on Sep-Pak florisil cartridges and determination by HPLC/FLD(High Performance Liquid Chromatography/Fluorescence Detector). Overall method recoveries for 8 PAHs spiked into these products ranged from 90 to 106%.
Keywords
PAHs; benzo(a)pyrene; alkali digestion; fish; HPLC/FLD;
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