• Journal of Microbiology
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• v.44 no.2
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• pp.217-225
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• 2006

Kaposi's sarcoma-associated herpesvirus (KSHV) RTA transcription factor is recruited to its responsive elements through interaction with RBP-Jk that is a downstream transcription factor of the Notch signaling pathway that is important in development and cell fate determination. This suggests that KSHV RTA mimics cellular Notch signal transduction to activate viral lytic gene expression. Here, I demonstrated that unlike other B lymphoma cells, KSHV -infected primary effusion lymphoma BCBL1 cells displayed the constitutive activation of ligand-mediated Notch signal transduction, evidenced by the Jagged ligand expression and the complete proteolytic process of Notch receptor I. In order to investigate the effect of Notch signal transduction on KSHV gene expression, human Notch intracellular (hNIC) domain that constitutively activates RBP-Jk transcription factor activity was expressed in BCBL1 cells, TRExBCBL1-hNIC, in a tetracycline inducible manner. Gene expression profiling showed that like RTA, hNIC robustly induced expression of a number of viral genes including KS immune modulatory gene resulting in downregulation of MHC I and CD54 surface expression. Finally, the genetic analysis of KSHV genome demonstrated that the hNIC-mediated expression of KS during viral latency consequently conferred the downregulation of MHC I and CD54 surface expression. These results indicate that cellular. Notch signal transduction provides a novel expression profiling of KSHV immune deregulatory gene that consequently confers the escape of host immune surveillance during viral latency.

• The Journal of Asian Finance, Economics and Business
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• v.7 no.10
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• pp.171-182
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• 2020

This research focuses on the importance of corporate social responsibility in building the company's reputation. Experts have studied CSR as an antecedent of a company's reputation, but the mechanisms underlying this process are rarely explored. Therefore, to fill this research gap, we demonstrate CSR's implementation combined with organizational justice based on discrepancy and equity theory. This study involved 210 employees in a family company. The study's analysis method uses Structural Equation Model (SEM), SmartPLS, with a five-step measurement and analysis procedure. The variables in this study are CSR implementation, organizational justice, employee trust, firm reputation, organizational objectiveness, organizational commitment, job satisfaction, and job performance. The results found that some of the direct relationships stated were not significant, but all demonstrations of indirect links were substantial. Besides, optimal CSR and organizational justice provide a reliable and positive domino effect in increasing the role and consequences of employee trust and firm reputation. The findings in this study confirm that upstream-downstream job performance causality can be successfully achieved if job satisfaction has been realized, job satisfaction can be achieved if organizational commitment can also be recognized, and organizational commitment can be developed. Reflection and influence, rather than organizational attractiveness becomes essential.

• Wind and Structures
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• v.22 no.6
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• pp.663-684
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• 2016

Conical vortices generated at the corner regions of large-span flat roofs have been investigated by using the Particle Image Velocimetry (PIV) technique. Mean and instantaneous vector fields for velocity, vorticity, and streamlines were measured at three visual planes and for two different flow angles of $15^{\circ}$. The results indicated that conical vortices occur when the wind is not perpendicular to the front edge. The location of the leading edge corresponding to the negative peak vorticity and maximum turbulent kinetic energy was found at the center of the conical vortex. The wind pressure reaches the maximum near the leading edge roof corner, and a triangle of severe suctions zone appears downstream. The mean pressure in uniform flow is greater than that under turbulent flow condition, while a significant increase in the fluctuating wind pressure occurs in turbulent streams. From its emergence to stability, the shape of the vortex cross-section is nearly elliptical, with increasing area. The angle that forms between the vortex axis and the leading edge is much smaller in turbulent streams. The detailed flow structures and characteristics obtained through FLUENT simulation are in agreement with the experimental results. The three dimensional (3-D) structure of the conical vortices is clearly observed from the comprehensive arrangement of several visual planes, and the inner link was established between the vortex evolution process, vortex core position and pressure distribution.

• Journal of the Society of Naval Architects of Korea
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• v.51 no.1
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• pp.88-98
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• 2014

A supercavitation is modern technology that can be used to reduce the frictional resistance of the underwater vehicle. In the process of reaching the supercavity condition which cavity envelops whole vehicle body, a vehicle passes through transition phase from fully-wetted to supercaviting operation. During this phase of flight, unsteady hydrodynamic forces and moments are created by partial cavity. In this paper, analytical and numerical investigations into the dynamics of supercavitating vehicle in transition phase are presented. The ventilated cavity model is used to lead rapid supercavity condition, when the cavitation number is relatively high. Immersion depth of fins and body, which is decided by the cavity profile, is calculated to determine hydrodynamical effects on the body. Additionally, the frictional drag reduction associated by the downstream flow is considered. Numerical simulation for depth tracking control is performed to verify modeling quality using PID controller. Depth command is transformed to attitude control using double loop control structure.

• Journal of the Korean Society of Propulsion Engineers
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• v.14 no.2
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• pp.1-9
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• 2010

LES(Large eddy simulation) of breakup and droplet atomization of a liquid jet into cross turbulent flow was performed. Two phase flow of gas and liquid phases were modeled by the mixed numerical scheme of both Eulerian and Lagrangian methods for gas and liquid droplet respectively. The breakup process of a liquid column and droplets was observed by implementing the blob-KH wave breakup model. The penetration depth into cross flow was comparable with experimental data for several variants of the liquid-gas momentum flux ratio by varying liquid injection velocity. SMD(Sauter Mean Diameter) distribution downstream of jet was analyzed.

• Korean Journal of Air-Conditioning and Refrigeration Engineering
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• v.23 no.12
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• pp.799-804
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• 2011

Radioactivity of high concentrations have existed in the handling nuclear materials in hot cell of PIEF(Post Irradiation Examination Facility). The exhaust filtration system was enabled to process cylindrical filters by using a manipulator in the hot cell. By establishing a double filtration system with two filters, backup protection against leakage or failure of the first is provided by the second filter. Additionally, this a arrangement is arrange intended to increase the total filtration efficiency. The result of the pressure drop changing in the air flow of the cylindrical and HEPA filters is observed by a curved line. A filtering efficiency of more than 99.99% to $0.3{\mu}m$ particle appears in the upstream and downstream during the efficiency test on the HEPA filters. The V-pleats type had a lower pressure drop than the separator type. There was no damage during usage and was found to be suitable with high capacity of air volume. Therefore, by carrying out performance tests of the exhaust filtration system, efficiency and safety can be achieved.

• Transactions of the Korean Society of Mechanical Engineers B
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• v.26 no.2
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• pp.261-268
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• 2002

Turbulent flow past a square cylinder confined in a channel is numerically investigated by Large Eddy Simulation(LES). The main objectives of this study are to verify the experimental results of Nakagawa et al.[Exp. in Fluids, Vol. 27, 3, pp. 284∼294, 1999] by LES and to obtain related flow information in detail. The LES results obtained are in excellent agreement with the experiment both qualitatively and quantitatively. The passive paticles numerically released into the flow field clearly show the barman vortex street. However, the vortices shed from the cylinder are significantly affected by the presence of the plates. Futhermore, periodic and alternating vortex-rollups are observed in the vicinity of the plates. The rolled-up vortex is convected downstream together with the corresponding Karman vortex forming a counter-rotating vortex pair. It is also revealed that the cylinder greatly enhances mixing process of the flow.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2008.03a
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• pp.320-329
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• 2008

The influence of thermodynamic transition associated with transcritical nitrogen injection upon the flow structure was investigated to explore numerical simulation of the injectant dynamics of oxygen/hydrogen coaxial jet in liquid rocket engines. Single and coaxial nitrogen jets were treated by comparing the transcritical and perfect-gaseous conditions, wherein the numerical model was accommodative to the real-fluid thermodynamics and transport properties at supercritical pressures. The model was in the first place validated by comparing the results of transcritical nitrogen injection between calculations and available experiments. For a single jet under the transcritical condition, the nitrogen kept a relatively high density up to its pseudo-critical temperature inside the mixing layer, since it remains less expanding until heated up to its pseudo-critical temperature. Numerical analysis revealed that cryogenic jets exhibit strong dependence of specific enthalpy profile upon the associated density profile that are both dominated by turbulent thermal diffusion. In the numerical model, therefore, exact evaluation of turbulent heat fluxes becomes very important for simulating turbulent cryogenic jets under supercritical pressures. Concerning the coaxial jets due to transcritical/gaseous nitrogen injections, the density profile inside the mixing layer was again affected by the thermodynamic transition of nitrogen. However, hydrodynamic instability modes of the inner jet did not show significant differences by this thermodynamic transition, so that further study is needed for the mixing process downstream of the near injection position.

• Food Engineering Progress
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• v.21 no.3
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• pp.191-200
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• 2017

There have been great efforts to develop a rapid and sensitive detection method to monitor the presence of pathogenic bacteria in food. While a number of methods have been reported for bacterial detection with a detection limit to a single digit, most of them are suitable only for the bacteria in pure culture or buffered solution. On the other hand, foods are composed of highly complicated matrices containing carbohydrate, fat, protein, fibers, and many other components whose composition varies from one food to the other. Furthermore, many components in food interfere with the downstream detection process, which significantly affect the sensitivity and selectivity of the detection. Therefore, isolating and concentrating the target pathogenic bacteria from food matrices are of importance to enhance the detection power of the system. The present review provides an introduction to the representative sample preparation strategies to isolate target pathogenic bacteria from food sample. We further describe the nucleic acid-based detection methods, such as PCR, real-time PCR, NASBA, RCA, LCR, and LAMP. Nucleic acid-based methods are by far the most sensitive and effective for the detection of a low number of target pathogens whose performance is greatly improved by combining with the sample preparation methods.

• Mycobiology
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• v.46 no.4
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• pp.429-439
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• 2018

To develop a convenient promoter analysis system for fungi, a null-pigment mutant (NPG) of Aspergillus nidulans was used with the 4'-phosphopantetheinyl transferase (PPTase) gene, npgA, which restores the normal pigmentation in A. nidulans, as a new reporter gene. The functional organization of serially deleted promoter regions of the A. nidulans trpC gene and the Cryphonectria parasitica crp gene in filamentous fungi was representatively investigated to establish a novel fungal promoter assay system that depends on color complementation of the NPG mutant with the PPTase npgA gene. Several promoter regions of the trpC and crp genes were fused to the npgA gene containing the 1,034-bp open reading frame and the 966-bp 3' downstream region from the TAA, and the constructed fusions were introduced into the NPG mutant in A. nidulans to evaluate color recovery due to the transcriptional activity of the sequence elements. Serial deletion of the trpC and crp promoter regions in this PPTase reporter assay system reaffirmed results in previous reports by using the fungal transformation step without a laborious verification process. This approach suggests a more rapid and convenient system than conventional analyses for fungal gene expression studies.