• Biomedical Science Letters
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• v.19 no.4
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• pp.348-352
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• 2013

microRNAs (miRNAs) play pivotal roles in controlling cell proliferation and differentiation. miRNA expression in human is becoming recognized as a new molecular mechanism of carcinogenesis. microRNA-34a (miR-34a), a member of the p53 network, was found to be regulated in multiple types of tumor. The purpose of this study was to define roles of miR-34a expression in cervical intraepithelial neoplasia with human papillomavirus infection, and its relationship with p53 protein expression. This study was performed to analyze expression of miR-34a by using qRT-PCR, and to evaluate p53 protein expression by using immunohistochemistry in 40 cases. Down-regulation of miR-34a expression was detected in 27 (67.5%) out of 40 cases and Immunoreactivity for p53 was found in 17 (42.5%) out of 40 cases. Nineteen (82.6%) of the 23 cases with a negative p53 expression showed a down-regulation miR-34a expression, there was a significant associations between miR-34a and p53 protein expression (P=0.04). These results suggest that miRNA-34a expression tend to be reduced depending on the advanced histologic grade, and down-regulation of miR-34a expression might be associated with inactivation of p53 protein expression by human papillomavirus infection.

• International Journal of Highway Engineering
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• v.10 no.4
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• pp.171-180
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• 2008

This study was conducted to analyze the curling behavior of concrete slabs on grade under temperature loading through the laboratory tests and to investigate the effects of the support conditions of underlying layers on the curling behavior of the slab on grade systems. For the laboratory tests, the concrete slabs were fabricated and the temperature measurement sensors were installed. The temperature loading was applied intentionally and the time histories of the vertical displacements of the slab at several different positions were measured. One-layered underlying layers were made using different materials and different thicknesses. Two-layered underlying layers were also made to have the same composite stiffness each other, but the material of the upper layer was designed to be different The experimental analysis results showed that the trend of the displacement time histories of the slab was basically the same as that of the temperature gradient time histories. The characteristics of the curling behavior of the slab were analyzed by separating the regions of curling up and curling down. The difference in the vertical displacements at the center and comer decreased as the stiffness of the underlying layer became larger, and the upper layer's material of the underlying layers affected the curling behavior of the slab.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.5
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• pp.1757-1760
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• 2012

Breast cancer is a major cause of death in women worldwide. Mammary tissue expressing xenobiotic metabolizing enzymes metabolically activate or detoxify potential genotoxic breast carcinogens. Deregulation of these xenobiotic metabolizing enzymes is considered to be a major contributory factor to breast cancer. The present study is focused on the expression of the xenobiotic metabolizing gene, CYP1A1, in breast cancer and its possible relationships with different risk factors. Twenty five tumors and twenty five control breast tissue samples were collected from patients undergoing planned surgery or biopsy from different hospitals. Semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR) and western-blotting were used to investigate the expression of CYP1A1 in breast cancer control and disease samples. mRNA expression of CYP1A1 was down-regulated in 40% of breast tumor samples. Down-regulation was also observed at the protein level. Significnat relations were noted with marital status and tumour grade but not histopathological type. In conclusion, CYP1A1 protein expression was markedly reduced in tumor breast tissues samples as compared to paired control tissue samples.

• Journal of the Korean Society of Radiology
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• v.6 no.5
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• pp.427-433
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• 2012

This study aimed at identifying changes in the acromiohumeral distance (AHD) and arm when the caudal gliding gradeII and III of Kaltenborn-Evjenth Concept$^{(R)}$ are applied to the right glenohumeral joint. The humeral head moved down about 5mm from the initial position when the gliding gradeII was applied, and about 8mm from the initial position when the gliding gradeIII was applied. Although men showed a higher acromiohumeral distance per grade than women in comparisons by gender for the acromiohumeral distance, there was no significant difference in statistics. The Abduction angle improved about $10^{\circ}$ from the initial angle when the gliding gradeII was applied, and about $12^{\circ}$ from the initial angle when the gliding gradeIII was applied. Although women showed the abduction angle greater than men for every grade in comparisons by gender for the abduction angle, there was no significant difference in statistics. Based on the aforesaid findings, the extent of kinematic changes in the humeral head could be identified when the gliding grades were applied. Accordingly, it is considered that more scientific evidence based treatments could be expected if influences on the surrounding structures by these changes could be learned through more studies in the future.

• Journal of the Korean Society of Safety
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• v.27 no.4
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• pp.1-6
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• 2012

Recently the government declared an all-out war on bogus fuel in order to crack down on tax evasion and ensure fuel safety. The move came after four people were killed in explosion at the two gas station. Illegally processed gasoline is the only one of low grade fuels. The problems are induced by relatively high vapor pressure of bogus fuel.

• Proceedings of the KIEE Conference
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• 1999.07b
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• pp.671-673
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• 1999

In order to cope with the occurrence of abnormal operation, rotatory condition monitoring system for guide roller of finishing train in wire rolling has been developed. In this study, proximity sensor and its holding devices that could overcome severe in-situ measurement conditions was designed and MMI software using C++ was programmed. Performance of the developed system turned out to be good enough as results of in-line test for POSCO #3 wire rolling mill. It is expected to contribute to prevent the cobble and the grade-down of products caused by the abnornormal operation of guide rollers.

• Asian Pacific Journal of Cancer Prevention
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• v.17 no.7
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• pp.3437-3445
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• 2016

Background: There is an increasing concern in the role of microRNA (miRNA) in the pathogenesis of bone metastasis (BM) secondary to prostate cancer (CaP). In this exploratory study, we hypothesized that the expression of vinculin (VCL) and chemokine X3C ligand 1 (CX3CL1) might be down-regulated in clinical samples, most likely due to the post-transcriptional modification by microRNAs. Targeted genes would be up-regulated upon transfection of the bone metastatic prostate cancer cell line, PC3, with specific microRNA inhibitors. Materials and Methods: MicroRNA software predicted that miR-21 targets VCL while miR-29a targets CX3CL1. Twenty benign prostatic hyperplasia (BPH) and 16 high grade CaP formalin-fixed paraffin embedded (FFPE) specimens were analysed. From the bone scan results, high grade CaP samples were further classified into CaP with no BM and CaP with BM. Transient transfection with respective microRNA inhibitors was done in both RWPE-1 (normal) and PC3 cell lines. QPCR was performed in all FFPE samples and transfected cell lines to measure VCL and CX3CL1 levels. Results: QPCR confirmed that VCL messenger RNA (mRNA) was significantly down-regulated while CX3CL1 was up-regulated in all FFPE specimens. Transient transfection with microRNA inhibitors in PC3 cells followed by qPCR of the targeted genes showed that VCL mRNA was significantly upregulated while CX3CL1 mRNA was significantly down-regulated compared to the RWPE-1 case. Conclusions: The down-regulation of VCL in FFPE specimens is most likely regulated by miR-21 based on the in vitro evidence but the exact mechanism of how miR-21 can regulate VCL is unclear. Up-regulated in CaP, CX3CL1 was found not regulated by miR-29a. More microRNA screening is required to understand the regulation of this chemokine in CaP with bone metastasis. Understanding miRNA-mRNA interactions may provide additional knowledge for individualized study of cancers.

• Journal of Powder Materials
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• v.5 no.4
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• pp.265-272
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• 1998

For the purpose of investigating the effect of sintering atmosphere and carbon addition on sintered density and microstructural characteristics, the M3/2 grade high speed steel powders with the addition of carbon are sintered in vacuum and $20%H_2/79%N_2/l%CH_4$ gas atmosphere. With the addition of 0 wt%C, 0.45wt%C and 1.15 wt%C the optimum sintering temperatures decrease down to $1260^{\circ}C$, $1210^{\circ}C$ and $1150^{\circ}C$ respectively for the vacuum sintered specimen, and also decrease down to $1130^{\circ}C$, $1120^{\circ}C$ and $1115^{\circ}C$ for the gas sintered specimen. The threshold temperatures for full densification decrease steeply with increasing carbon content of the sintered specimen, while this temperatures are slowly decreased at high carbon content. The vacuum sintered specimen shows the primary carbides of MC and $M_6C$ type at the optimum sintering temperature, and eutectic carbides of $M_2C$ and Fe-Cr type are produced in the oversintered specimen. The gas sintered specimen exhibits M6C and Fe-Cr type primary carbides at the optimum sintering temperature. The eutectic carbides of $M_6C$ and Fe-Cr type and MX type carbonitride are shown for the oversintered specimen in the gas atmosphere. The hardness of gas sintered specimen shows high value of 830-860 Hv due to the increment of carbide precipitation.

• Physical Therapy Korea
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• v.14 no.1
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• pp.55-63
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• 2007

This study was designed to examine the relationship between clinical symptoms, self-efficacy, and performance of women with osteoarthritis. It is a survey study of 60 women who were diagnosed as osteoarthritis and given medical treatments from September, 2005 to October, 2005 in hospital 'H' located in Yongin-si. For clinical symptoms, radiographs of the subjects' knees were taken and evaluated the pathology grade by the Kellgren-Lawrence grade. Pain and stiffness was measured by the measure of WOMAC (Western Ontario and McMaster Universities Osteoarthritis Index), and functional reach test was measured in order to examine balancing abilities. Self-efficacy was measured by a tool which has revised the ASES (Arthritis Self-Efficacy Scale), and performance was measured by recording the difficulty of the WOMAC measurements themselves, and the time taken for 20 m walking, going up and down 9 stairs, and 5 sit-down and stand-up repetitions. The resulting differences in the other variables according to performance and the relationship between performance with variables are the following. First, an increase in pain in women with osteoarthritis led to decreased functional ability. Second, an increase in stiffness in women with osteoarthritis led to a decrease in functional ability. Third, a decrease in balance in women with osteoarthritis led to a decrease in functional ability. Fourth, a decrease in self-efficacy in women with osteoarthritis led to a decrease in functional ability. Fifth, the variables for estimating the performance by self-report were pain and self-efficacy. The variables for estimating the performance by recording the time taken was balance and self-efficacy. As a result factors such as pain, balance and self-efficacy in women with osteoarthritis were closely related to performance. Based on the results, it seems that physical therapy programs to decrease pain and to increase the balance in women with osteoarthritis, and psychological approaches to increasing self-efficacy are needed. I hope that the results of this study will be useful data for clinical management and intervention for women with osteoarthritis.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.14 no.1
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• pp.184-193
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• 2013

This study examined a correspondence of children's anticipated vocations, perceived competencies, and interests. For the purpose, this study asked 659 primary school students what activities they liked, those they thought they were good at, and what job they expected when they grew up. The responses were coded into RIASEC Holland codes and agreement across the three questions was examined using kappa statistics. Results indicated that there was a correspondence of children's anticipated vocations, perceived competencies, and interests. When broken down into separate examinations by gender, males and females had significant kappas for agreement between vocation and competence. And the correspondence of competence and interest was significant for males too. We couldn't examine females' case because they didn't answer C-type of Holland. Examinations of the results by grade demonstrated that 5th-grade and 6th-grade had significant kappas for agreement between vocation and competence. However the correspondence of vocation and interest was significant for 5th-grade. the correspondence of competence and interest was significant for 5th-grade too. We couldn't examine 6th-grade's case because they didn't answer C-type of Holland. The result obtained from this study indicate that there is a correspondence of children's anticipated vocations, perceived competencies, and interests. Such a result means that children, 5th-grade and 6th-grade, can consider their competencies and interests with their vocations. The findings are especially relevant for counselors and teachers working with children.