• IMMUNE NETWORK
• /
• v.2 no.4
• /
• pp.217-222
• /
• 2002

Background: Scid.adh is a recently developed murine thymic lymphoma cell line, which has been used as in vitro model for the study of double negative stage III thymocytes. In this study, we compared the expression profile of a number of genes and proteins, which are tightly related to T cell development and apoptosis, in thymic lymphoma cell lines, R1.1, EL-4, and Scid.adh for the developmental staging. Methods: We examined the expression of development marker genes and proteins in three lymphoma cell lines by flow cytometry and RT-PCR. In addition, the expression of apoptosis-related molecules including bcl-2, bax and Fas was also investigated. Results: As previously reported, Scid.adh cell line expressed CD8 and CD25 but not TCR ${\alpha}$ chain, while R1.1 cells expressed TCR ${\alpha}$ chain and both CD4 and CD8 transcripts. These suggest that R1.1 might be in double positive stage, and low level of CD44 expression and the absence of CD25 support this suggestion. In contrast, EL-4 cells showed high level of TCR ${\alpha}$ chain transcript, and low-level of CD4 expression, suggesting that EL-4 is in more mature stage than R1.1. Further, this suggestion was supported by the lack of mT-20 in EL-4 cells, which is expressed in the immature thymocytes, and Scid.adh and R1.1 cell lines, but not in the terminally differentiated thymocytes and peripheral T cells. Among the apoptosis-related gene, transcripts of bcl-2 gene were detected in both R1.1 and EL-4 but not in Scid.adh cells, while bax was expressed in all cell lines. Fas expression was the highest in EL-4 cells and low in Scid.adh cell line. Conclusion: R1.1 cell may represent double positive stage, and EL-4 is more differentiated cell line. In addition, Scid.adh and EL-4 cell lines are suspected to be useful for the study of function of bcl-2 family and Fas during the thymocyte development, respectively.

• KOREAN JOURNAL OF CROP SCIENCE
• /
• v.44 no.4
• /
• pp.321-329
• /
• 1999

The effect of water deficits on soybean [Glycine max (L.) Merr.] could appear on seed quality through changes of morphological plant characteristics. Two Korean genotypes, Hwangkeum (determinate growth habit) and Muhan (indeterminate growth habit), were used to examine the influences of treatment stage and method of water deficit during reproductive growth period on yield and seed quality of soybean. Water deficit at R5 or R6 stages was as damaging to seed quality as double water-deficit treatments at R2+R5 or R2+R6. However, seed from double water-deficit treatment tended to have lower oxidation-reduction potential compare to the corresponding single water-deficit treatment. In comparison with Muhan, Hwangkeum had significantly greater oxidation-reduction potential value. Seed yield per plant in both genotypes depended greatly on seed yield of branches. However, the proportion of number of branch seed to total seed umber in Hwangkeum was increased as the water deficit was applied during later reproductive stage, whereas, in Muhan the proportion was lower. Water-deficit treatments including the single and double water-deficit treatments and non-stressed treatment were able to be classified into five groups for Hwangkeum and four groups for Muhan based on the influences on yield components, number of pod, number of seed, and single seed weight, using principal component analysis. In both genotypes, R2+R5 water-deficit treatment decreased number of pod and seed, but increased single seed weight. On the contrary, R6 or R2+R6 stress increased the pod and seed number, but decreased single seed weight.

• Elastomers and Composites
• /
• v.55 no.1
• /
• pp.51-58
• /
• 2020

In this study, the thermal oxidation of raw natural rubber (NR) was investigated under controlled conditions by optical image and fourier transform infrared (FT-IR) analysis. The thermal oxidation was performed on a transparent thin film of raw NR coated on a KBr window in a dark chamber at 80℃ under low humidity conditions to completely exclude moisture and restrict light oxidation. Images of the thin film of raw NR were obtained before and after thermal oxidation. FT-IR absorption spectra were measured in the transmission mode at different thermal exposure times. The thermal oxidation of NR was examined by the changes in the absorption peaks at 3449, 1736, 1447, 1377, 1242, 1072, and 833 cm^-1, which corresponded to a hydroxyl group (-OH), a carbonyl group (-C=O) from an aldehyde and a ketone, a methylene group (-CH₂-), a methyl group (-CH₃), a carbon-oxygen single bond (-C-O) from an epoxide, a carbon-oxygen bond (-C-O) from an ether, an alcohol, a peroxide, or a cyclic peroxide, and a cis-methine group (cis-CCH₃=CH-), respectively. In the initial stage of thermal oxidation, two different types of free radicals were produced quickly and randomly by the homolytic cleavage of a double bond and allylic hydrogen abstraction. Aldehydes and ketones were formed from chain scissions of the double bonds and alcohols were produced from allylic hydrogen abstraction at the methylene or methyl groups. Two reactions seemed to proceed competitively with each other. At a later stage, oxidative crosslinks seemed to dominate through the combination of free radicals such as an allyl radical (CH=CHCH₂·), alkoxy radical (RO·), and peroxy radical (ROO·) and the reaction of a hydroperoxide (-ROOH) with a double bond. The image obtained after thermal oxidation showed hardening without cracks. Based on these observations, a plausible two-step mechanism was suggested for chain hardening caused by the thermal oxidation.

• Advances in concrete construction
• /
• v.1 no.3
• /
• pp.239-252
• /
• 2013

Mortar microstructure is considered as a three-phase composite material, which is cement paste, fine aggregate and interfacial transition zone. Interfacial transition zone is the weakest link between the cement paste and fine aggregate, so it has a significant role to determine the properties of cementitious composites. In this study, specimens (w/c = 0.35, 0.45, 0.55) with various volume fractions of fine aggregate ($V_f$ = 0, 0.1, 0.2, 0.3 and 0.4) were cast and tested. To predict the equivalent migration coefficient ($M_e$) and migration coefficient of interfacial transition zone ($M_{itz}$), double-inclusion method and Mori-Tanaka theory were used to estimate. There are two stages to estimate and calculate the thickness of interfacial transition zone (h) and migration coefficient of interfacial transition zone ($M_{itz}$). The first stage, the data of experimental chloride ion migration coefficient ($M_s$) was used to calculate the equivalent migration coefficient of fine aggregate with interfacial transition zone ($M_e$) by Mori-Tanaka theory. The second stage, the thickness of interfacial transition zone (h) and migration coefficient of interfacial transition zone ($M_{itz}$) was calculated by Hori and Nemat-Nasser's double inclusion model. Between the theoretical and experimental data a comparison was conducted to investigate the behavior of interfacial transition zone in mortar and the effect of interfacial transition zone on the chloride migration coefficient, the results indicated that the numerical simulations is derived to the $M_{itz}/M_m$ ratio is 2.11~8.28. Additionally, thickness of interfacial transition zone is predicted from $10{\mu}m$, 60 to $80{\mu}m$, 70 to $100{\mu}m$ and 90 to $130{\mu}m$ for SM30, M35, M45 and M55, respectively.

• Korean Journal of Soil Science and Fertilizer
• /
• v.48 no.5
• /
• pp.542-548
• /
• 2015

This study investigated methane productions and a degradation rate of organic matters by German standard method, VDI4630 test. In this study, 4 livestock byproducts from livestock farm were selected for the investigation. The objective of this study was to estimate a distribution of organic matters by using the double first-order kinetics model in order to calculate the rate of biodegradable organic matters which degrade rapidly in the initial stage and the persistently biodegradable organic matters which degrade slowly later. As a result, all the byproducts applied in this study showed rapid decomposition in the initial stage. Then the decomposition rate began to slow down for a certain period and the rate became 5 times slower than the initial decomposition rate. This trend of decomposition rate changes is typical conditions of organic matter decompositions. The easily degradable factors ($k_1$) ranged between $0.145{\sim}0.257day^{-1}$ and persistent degradable factors ($k_2$) were $0.027{\sim}0.080day^{-1}$. Among these results, greater organic matter decomposition rates from VDI4630 had greater $k_1$ values (0.257, $0.211day^{-1}$) and smaller $k_2$ values (0.027, $0.030day^{-1}$) for dairy wastewater and forage byproduct, respectively.

• Membrane Journal
• /
• v.32 no.2
• /
• pp.140-149
• /
• 2022

In this study, the conditions of low temperature and high pressure of biogas upgrading process using polysulfone membrane have been designed and tested to achieve the high recovery and efficiency corresponding to those of the highly selective polymeric materials. Polysulfone hollow fiber membrane with 4-component dope solution was spun via non-solvent induced phase separation. The hollow fiber membrane was mounted into a 1.5 inch housing. The effective area was 1.6 m², and its performance was examined in various operation temperatures and pressures. CO₂ and CH₄ permeances were 412 and 12.7 GPU at 20℃, and 280 and 3.6 GPU at -20℃, respectively, while the CO₂/CH₄ selectivity increased from 32.4 to 77.8. Single gas test was followed by the mixed gas experiments using single-stage and double stage where the membrane area ratio varied from 1:1 to 1:3. At the single-stage, CH₄ purity increased and the recovery decreased as the stage-cut increased. At the double stage, the area ratio of 1:3 showed the higher CH₄ recovery as decreasing the operation temperature at the same purity of CH₄ 97%. Finally, polysulfone hollow fiber membranes have yielded of both CH₄ purity and recovery of 97% at -20℃ and 16 barg.

• Korean Journal of Soil Science and Fertilizer
• /
• v.45 no.4
• /
• pp.503-510
• /
• 2012

Microwave radar can penetrate cloud cover regardless of weather conditions and can be used day and night. Especially a A ground-based polarimetric scatterometer operating at multiple frequencies can continuously monitor the crop conditions. We analyzed scattering characteristics of rice and soybean using pauli decomposition method. Surface scattering (${\alpha}$) is the dominant component over the entire stages for all bands and pauli decomposition value was the highest for L-band. Double bounce scattering (${\beta}$) and volume scattering (${\gamma}$) were approximately equal for C-band and volume scattering was higher than double bounce scattering for X-band in rice field. In soybean, double bounce scattering becomes higher than volume scattering during the R2 stage (DOY 224) and there was a significant difference between the two components after the R4 stage (DOY 242) for L-band. The maximum growth stage of soybean can also be detected using L-band double bounce scattering. The peak of double bounce effect coincides with the peak of growth biophysical variables on DOY 271. We found that pauli decomposition can provide insight on the relative magnitude of different scattering mechanisms during the rice and soybean growth cycle.

• Proceedings of the KIEE Conference
• /
• 1998.07g
• /
• pp.2515-2517
• /
• 1998

The micro fresnel lens(MFL) was modeled and fabricated on a XY-stage electrostatically driven by comb actuator. The modeled MFL was approximated as a step shape with 4-phase and 4-zone plate. The focal length and diameter of the MFL is 20mm and 912${\mu}m$, respectively. The XY-stage suspending the MFL is designed to generate a large static displacement up to about 20${\mu}m$. On SOI substrates, we first fabricated MFL using the RIE(reactive Ion etching) technology and then patterned and etched bulk silicon to make XY-stage. After the fabrication of all structures on top side of the SOI substrates. $Si_3N_4$ was deposited for passivation of all structures using PECVD(plasma enhanced chemical vapor deposition). All the MFL systems width comb drive actuator were released by KOH etching from the bottom side of the SOI wafer using double-sided alignment technique. In fabrication of MFL, a dry etching conditions is established in order to improve surface roughness and to control the etched depth.

• Journal of Sensor Science and Technology
• /
• v.11 no.5
• /
• pp.255-262
• /
• 2002

Human retina is able to detect the edge of an object effectively. We designed a CMOS vision chip by modeling cells of the retina as hardwares involved in edge detection. There are several fluctuation factors which affect characteristics of MOSFETs during CMOS fabrication process and this effect appears as output offset of the vision chip which is composed of pixel arrays and readout circuits. The vision chip detecting edge information from input image is used for input stage of other systems. Therefore, the output offset of a vision chip determine the efficiency of the entire performance of a system. In order to eliminate the offset at the output stage, we designed a vision chip by using CDS(Correlated Double Sampling) technique. Using standard CMOS process, it is possible to integrate with other circuits. Having reliable output characteristics, this chip can be used at the input stage for many applications, like targe tracking system, fingerprint recognition system, human-friendly robot system and etc.

• Journal of the Korea Institute of Information and Communication Engineering
• /
• v.8 no.4
• /
• pp.826-832
• /
• 2004

A CMOS 8 bit folding and interpolating ADC for an embedded system inside VLSI is presented in this paper. This folding ADC uses the 2 stage architecture for improving of nonlinearity. repeating the folding and interpolating twice. At a proposed structure, a transistor differential pair operates on the second folder. A ADC with 2 stage architecture reduces the number of comparators and resisters. So it is possible to provide small chip size, low power consumption and high operating speed. The design technology is based on fully standard 0.25m double-Poly 2 metal n-well CMOS Process. The simulated Power consumption is 45mW with an applied voltage of 2.5V and sampling frequency of 250MHz. The INL and DNL are within <ㅆㄸㅌ>$\pm$0.2LSB, respectively. The SNDR is approximately 45dB for input frequency of 10MHz.