• 한국자원식물학회지
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• 제33권6호
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• pp.638-644
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• 2020

Lilium lancifolium (LL) is widely cultivated in East Asia and used to attenuate airway diseases. Our current study aimed to investigate the therapeutic effect of LL on pain level and inflammatory response in a rat model of monosodium iodoacetate (MIA)-induced osteoarthritis (OA). We first examined the effect of LL on inflammatory cytokines and inflammatory mediators in IL-1β-treated HTB-94 cells. The LL extract was found to significantly inhibit the levels of Interleukin-6 (IL-6), Interleukin-8 (IL-8), and prostaglandin-E2 (PGE-2) in Interleukin-1 β (IL-1β)-stimulated HTB-94 cells in a dose-dependent manner. Moreover, chronic oral administration of LL effectively restored the weight-bearing distribution in the rat model of MIA-induced OA. In addition, administration of LL inhibited inflammatory cytokines and inflammatory mediators, such as C-reactive protein (CRP), IL-6, leukotriene B4 (LTB-4), PGE-2, and matrix metalloproteinase-9 (MMP-9). Our findings collectively suggested LL as one of the potential therapeutic agents for OA, owing to its properties of reducing pain and inflammatory responses.

• 대한의생명과학회지
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• 제26권2호
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• pp.66-74
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• 2020

This study was carried out to investigate the protective effect of crude saponin from Platycodon grandiflorum on Clinical chemical parameters in male rats acutely exposed to 2,3,7,8-tetrachlorodibenzo-ρ-dioxin (TCDD). Crude saponin was prepared from Korean Platycodon grandiflorum with Diaion HP-20 adsorption chromatography after extraction of 80% ethanol at 75℃. The crude saponin was confirmed by thin layer chrmatography. When compared with ginseng saponins, the crude saponin had both a few number of saponins and a broad distribution. Forty male rats (200±20 g) were divided into 4 groups. Normal control (NC) group received vehicle and saline; TCDD-treated (TT) group received TCDD (40 ㎍/kg, single dose) intraperitoneally; Platycodon grandiflorum saponin (PG5 and PG10) groups received crude saponin 5 mg/kg and 10 mg/kg (p.o), respectively, for 2 weeks before 1 week of TCDD-exposure. Increase of body weight was retarded greatly by TCDD-exposure. Body weight of animals in TT group was significantly decrease after 2 days of TCDD-exposure. However, body weights of animals in PG groups increased through the experimental perimental period, although the increasing rate was slower than that of NC group. Increases in contents of blood glucose, total cholesterol and triglyceride (TG) and activities of amylase, lipase, AST, ALT and LDH by toxic action of TCDD were significantly attenuated by crude saponin from Platycodon grandiflorum (P<0.05). In conclusion, these results suggest that crude saponin prepared from Korean Platycodon grandiflorum might be a member of useful protective agents against TCDD, which is one of the environmental hormones.

• Animal cells and systems
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• 제4권1호
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• pp.63-70
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• 2000

The distribution of receptor subtypes for endothelin (ET) in the kidney of the freshwater turtle, Amyda japonica, was examined by quantitative in vitro receptor autoradiography using iodinatd mammalian type ET-1 ($^125$/I-ET-1)as a radiolabeled ligand. Specific $^125$/I-ET-1 bindings were localized to renal tubules, renal arteries and ureter with binding densities of 111.21 $\pm$ 19.14, 182.13$\pm$10.57 and 219.46$\pm$12.83 amol/$mm^2$. respectively. Binding dissociation constants in renal tubules, renal arteries and ureter were 1.05 $\pm$ 0.63, 2.03 $\pm$0.56 and 1.70$\pm$0.47nM, respectively. Receptor subtypes for ET in the kidney were characterized by competition with BQ 123 and BQ 788 as specific antagonists for ET receptors, type A (ET$_A$ ), and type B (ET$_B$) subtypes, respectively. Specific $^125$/I-ET-1 bindings in renal arteries and ureter were potently inhibited by BQ 123 in a dose-dependent manner, whereas BQ 788 was not in competing for specific $^125$/I-ET-1 bindings in this structure. However, specific $^125$/I-ET-1 bindings in renal tubules were inhibited more potently by BQ 788. Therefore, these results indicate that specific ET receptors are localized in renal tubules, renal arteries and the ureter of the freshwater turtle. Results also suggest that the predominant ET receptor subtypes are like the ETA receptor in renal arteries and ureter, and like the ET/$_A$ receptor in the renal tubule.

• 디지털융복합연구
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• 제13권1호
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• pp.331-339
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• 2015

사이버나이프치료에서 삽입된 금표지자의 삽입 개수 및 인식의 제한으로 인하여 3D DOF로 치료하는 경우 척추구조물 정렬을 가능하게 하는 Xsight spine tracking system으로 회전방향의 위치오차를 보정함으로서 표적위치오차를 개선하고, 치료방법의 대안을 제시하고자 한다. 실험결과 6D DOF에서 표적위치오차는 $0.214{\pm}0.058mm$, 종양내부에 삽입된 2개 금표지자를 이용한 3D DOF에서 $0.673{\pm}0.142mm$, 종양외부에 삽입된 2개 금표지자를 이용한 3D DOF에서 $1.126{\pm}0.253mm$, Xsight spine tracking system의 적용 한 3D DOF에서 $0.542{\pm}0.103mm$로 나타났다. 실험결과 척추 구조물로 회전방향에 대한 보정을 시행하였을 때 표적위치에 대한 정확성이 약 48% 향상되었다. 또한, 선량분포의 일치성도 약 3%가 향상되어 일치하는 것을 확인할 수 있었다. 따라서 척추구조물 정렬을 병용한 Xsight spine tracking system의 회전방향에 대한 보정은 유용한 것으로 평가 되었다.

• 한방안이비인후피부과학회지
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• 제22권1호
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• pp.62-75
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• 2009

Objective : The purpose of this study is to examine the effects of Gamisipjeontang-gamibang(GT) on an experimental bum elicitation. Method : The absorbance of the photo cell mixed with GT at the Abs 560 nm was measured after irradiation for 1 min. In order to know the antioxidant effect on skin cell of mice after burn elicitation, superoxide dismutase(SOD) activity was measured. Also, in order to know effects on the skin regeneration in the burned mice, we counted the eosinophil in blood from animals via cardiac puncture and observed the histological structure in the epidermal basal layer and the dermal section on the 3rd, 7th and 14th day after burn elicitation. We also studied changes in angiogenesis in the capillary surrounding the basal layer and dermal papilla. The changes of HSP70 distribution and changes of p53 positive reaction were observed to investigate the changes of the stress in the skin as well. Result : The results indicated that GT has a significant impact on the antioxidant effect on skin cells of mice after bum elicitation by increasing SOD activity in vitro test. GT seems to decrease MIP-2 which induces neutrophil infiltration and promotes the angiogenesis dose-dependently. Furthermore, GT decreased HSP70, the expression of which was induced by elevated temperatures, and p53 which induce a apoptosis after stress. Conclusion : GT can be applied to burned skin through its antioxidant effect and skin regeneration property.

• 한국지능시스템학회논문지
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• 제17권4호
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• pp.466-471
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• 2007

Otsu의 임계값 결정법을 포함한 기존의 임계값 결정 기법은 그레이 레벨 빈도수 히스토그램 정보를 이용하여 임계값을 결정한다. 그러나 빈도수 히스토그램은 입력 영상에서 그레이 레벨 빈도수 정보만을 재구성한 것이므로, 입력 영상의 그레이 레벨의 분포 및 그레이 레벨간의 관계성을 포함하지 않는다. 따라서 임계값 결정에 있어서 영상의 정보를 충분히 반영하지 못하여 때때로 부적절한 임계값을 제시한다. 본 논문에서는 빈도수 정보뿐만 아니라 그레이 레벨간의 상관관계함수를 정의하고, 정의된 상관관계함수를 이용하여 공간적 상관관계 정보 추출 및 추출된 정보로부터 임계값을 결정하는 기법을 제안한다. 제안된 기법의 타당성을 빈도수 히스토그램에 기반한 Otsu의 임계값 결정법과의 비교 실험을 통하여 보인다.

• The Korean Journal of Physiology and Pharmacology
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• 제16권3호
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• pp.153-158
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• 2012

Cellular effects of ethanol in YD-15 tongue carcinoma cells were assessed by MTT assay, caspase activity assay, Western blotting and flow cytometry. Ethanol inhibited the growth and proliferation of YD-15 cells in a dose- and time-dependent manner in an MTT assay. The effects of ethanol on cell cycle control at low percent range of ethanol concentration (0 to 1.5%), the condition not inducing YD-15 cell death, was investigated after exposing cells to alcohol for a certain period of time. Western blotting on the expression of cell cycle inhibitors showed that p21 and p27 was up-regulated as ethanol concentration increases from 0 to 1.5% whilst the cell cycle regulators, cdk1, cdk2, and cdk4 as well as Cyclin A, Cyclin B1 and Cyclin E1, were gradually down-regulated. Flow cytometric analysis of cell cycle distribution revealed that YD-15 cells exposed to 1.5% ethanol for 24 h was mainly arrested at G2/M phase. However, ethanol induced apoptosis in YD-15 cells exposed to 2.5% or higher percent of ethanol. The cleaved PARP, a marker of caspase-3 mediated apoptosis, and the activation of caspase-3 and -7 were detected by caspase activity assay or Western blotting. Our results suggest that ethanol elicits inhibitory effect on the growth and proliferation of YD-15 tongue carcinoma cells by mediating cell cycle arrest at G2/M at low concentration range and ultimately induces apoptosis under the condition of high concentration.

• Radiation Oncology Journal
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• 제4권2호
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• pp.165-172
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• 1986

전자선 치료시에 납판이나 저융점 합금이 조사면의 형태의 변형을 위하여 사용되고 있다. 콜리메이팅 장치와 마찬가지로 조사면 변형을 위한 물질도 전자선의 출력에 영향을 미친다. 저자들은 폴리스티렌 팬톰에 삽입된 Farmer형 전리함을 이용하여 Clinac-18의 전자선의 변형된 조사면에 대한 출력율을 측정하고 출력율에 영향을 미치는 요인들에 관해 분석했다. 전자선의 출력율은 전자선의 초기 에너지, 콜리메이팅 장치뿐만 아니라 조사면 크기에도 좌우되었다. 변형된 조사면에 대하여 잔자선의 에너지에 관계없이 X-선 콜리메이터와 전자선 어플리케이터의 조합이 고정되면 조사면의 크기 대 출력율은 A/P로 표시된 등가조사면의 크기에 따라 변하지만 조사면의 형태에 대해서는 무시할 수 있었다. 그러나 개조사면에 대한 출력율은 변형조사면의 출력율로부터 예상될 수 없고, 그것들만의 독립적인 관계를 가지고 있었다. 어플리케이터와 콜리메이터의 조합이 고정된 경우에 한해서 판자선의 변형조사면에 대한 출력율은 A/P로서 표시된 등가 조사면 방법에 의하여 구할 수 있다.

• 방사선산업학회지
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• 제6권2호
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• pp.139-145
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• 2012

Gold nanoparticles (GNPs) have led to the development of a new field in the diagnosis and treatment of diseases such as cancer. An efficient synthesis of gold nanoparticles within the range of 8~57 nm was established by ${\gamma}-ray$ irradiation. The good point of a radiation-based method is the production of gold nanoparticles with a higher concentration and narrower size distribution compared with conventional methods. The size of gold nanoparticles was controlled using two methods. : (i) varying the ${\gamma}-ray$ irradiation dose of 10 to 25 kGy and (ii) varying the concentration of $HAuCl_4$ solution from 4 to 40 mM. In addition, the GNPs were radiolabeled using $[^{125}I]NaI$ in a simple and fast manner with high yields. The produced gold nanoparticles were characterized using a transmission electron microscopy (TEM), a UV-visible spectrophotometer, and a radio-TLC imaging scanner. From these results, these radiolabeled GNPs can be applicable for a radioisotope tag of biomolecules.

• 한국지형학회지
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• 제24권3호
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• pp.149-159
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• 2017

Coastal dunes have many environmental and ecological socio-economic importances; various human activities, however, have impaired their structures and functions over the years. In 2016, environmental survey on coastal sand dune was carried out to report the present status and update the coastal sand dune list in 2001. This research analyzed the survey data in terms of geomorphological and socio-economic aspects. A list of the 189 coastal sand dune and information was updated by province. A 36.5% reduction in coastal sand dune area have been identified for using agricultural land, development area since the approximately 1940-50s. Development area in coastal sand dune covered small area as 5%, the use intensity was relatively hard. Furthermore, the difference by province also showed. Approximately 80% of the development area was concentrated in 20% of total sand dune several sand dune. It is generally accepted that coastal sand dunes were exposed high development pressure. However the result means that the general brief dose not apply for all sand dune. Therefore, characteristics of each sand dune should be analyzed and concerned the diverse management plans.