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Use of In Vivo and In Vitro Systems to Select Leishmania amazonensis Expressing Green Fluorescent Protein

  • Costa, Solange Dos Santos;Golim, Marjorie De Assis;Bergmann, Bartira Rossi;Costa, Fabio Trindade Maranhao;Giorgio, Selma
    • Parasites, Hosts and Diseases
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    • v.49 no.4
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    • pp.357-364
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    • 2011
  • Various Leishmania species were engineered with green fluorescent protein (GFP) using episomal vectors that encoded an antibiotic resistance gene, such as aminoglycoside geneticin sulphate (G418). Most reports of GFP-Leishmania have used the flagellated extracellular promastigote, the stage of parasite detected in the midgut of the sandfly vector; fewer studies have been performed with amastigotes, the stage of parasite detected in mammals. In this study, comparisons were made regarding the efficiency for in vitro G418 selection of GFP-Leishmania amazonensis promastigotes and amastigotes and the use of in vivo G418 selection. The GFP-promastigotes retained episomal plasmid for a prolonged period and G418 treatment was necessary and efficient for in vitro selection. In contrast, GFP-amastigotes showed low retention of the episomal plasmid in the absence of G418 selection and low sensitivity to antibiotics in vitro. The use of protocols for G418 selection using infected BALB/c mice also indicated low sensitivity to antibiotics against amastigotes in cutaneous lesions.

Daily Profiles of Blood Insulin, Insulin-Like Growth Factor-I, Thyroxine and Triiodothyronine in Ewes Under Three Levels of Feed Intake

  • Caldeira, R.M.;Vasques, M.I.;Vaz Portugal, A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.13 no.8
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    • pp.1121-1126
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    • 2000
  • Daily variation in the serum concentrations of insulin and insulin-like growth factor-I and in the plasma concentrations of thyroxine and triiodothyronine were evaluated in ewes fed 30%, 100% and 200% of theoretical maintenance energy requirements. The single daily meal has had significant effects (p<0.05) on almost all profiles. In general, serum or plasma hormone concentrations have increased after the meal, in particular at the two higher levels of energy intake. In the group submitted to the lowest level of energy intake, the consequences of the meal on circulating levels were almost imperceptible. The effects of feeding levels on serum or plasma concentrations have widely varied among hormones, not showing any objective pattern or relationship. Because these variations may affect the interpretation of these blood indicators, knowledge of daily profiles and of the effect of feed level must be considered. In order to maximize the diagnostic value of those indicators, the most suitable times for blood collection seem to be 16 h after the meal and (or) just before the meal. The collection 16 h after the meal apparently allows the characterization of a relatively steady metabolic state, intermediate between the close effects of food intake and the final phase of the intensification of body reserves mobilization. The collection just before the meal will give a good indication of the level of activity of those mobilization mechanisms.

Sweet Basil (Ocimum basilicum): much more than a condiment

  • Barbalho, Sandra Maria;Machado, Flavia Maria Vasques Farinazzi;Rodrigues, Jaqueline Dos Santos;Silva, Tiago Henrique Pereira Da;Goulart, Ricardo De Alvares
    • CELLMED
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    • v.2 no.1
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    • pp.3.1-3.5
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    • 2012
  • Ocimum basilicum belongs to the family Lamiaceae and can be found in tropical Asia, Africa, Central America and South America. It is widely cultivated commercially as a condiment or for the production of essential oil. In this review, this plant is accredited with important effects on health, in addition to being a condiment. In folk medicine it is used to treat vomiting, intestinal colic and diarrhea, menstrual pains and to improve kidney function. Some studies have demonstrated that it can be used for the treatment of hyperlipidemia and also that it has antioxidant, neuroprotective, anti-inflammatory and vasodilator properties as well as hepatoprotective effects. In view of its broad range of effects, further studies should be conducted to ascertain the possible use of this plant in clinical trials, not only for prevention but also as an adjuvant in the treatment of different diseases.

Effects of nursing intervention program on reducing acute confusion in hospitalized older adults (입원노인 환자를 위한 급성혼돈 간호중재 프로그램 효과)

  • Hwang, Hea-Jeong;Shin, Yeonghee
    • Korean Journal of Adult Nursing
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    • v.26 no.1
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    • pp.89-97
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    • 2014
  • Purpose: During hospitalization, confusion occurs in 15~20% of older adults and persists in 5~10% of them. This study was designed to investigate effects of a five-day nursing intervention program designed to reduce confusion in hospitalized older adults. Subjects were inpatients over the age of 60 years old and were admitted in a surgical care unit. Methods: Data were collected from 111 patients. Subjects divided into one of two groups, 58 patients of the control group which received the usual nursing care; and 53 patients of the intervention group which received the usual nursing care plus nursing intervention program for reducing acute confusion. The Delirium Observation Screening Scale (DOS) and the Korean Mini-Mental Screening Examination (MMSE-K) were utilized. Results: 1) Acute delirium was significantly reduced in the intervention group compared to the control group ($x^2$=4.22, p=.034) as well as the duration of the delirious state was significantly shortened (F=56.62, p<.001). Cognitive function of the intervention group was improved (F=21.14, p<.001). Conclusion: The nursing intervention program reduced the incidence and duration of acute delirium of the elderly inpatients, as well as it helps them keep better cognitive function than the control group.

Design and Implementation of C Program Detranslator from Symbol Table for Program Declaration Part (프로그램 선언부를 위한 심벌테이블에서 C 프로그램 역번역기의 설계 및 구현)

  • Kwon, Hyeok-Ju;Kim, Young-Koun;Lee, Yang-Sun;Oh, Se-Man
    • Proceedings of the Korea Information Processing Society Conference
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    • 2005.05a
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    • pp.553-556
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    • 2005
  • ANSI C 언어는 UNIX 시스템에서 뿐만 아니라 DOS 환경에서 수행되는 C 컴파일러와 각종 지원 도구가 개발되어 보급됨으로써 오늘날 널리 사용되는 범용 프로그래밍 언어 중 하나이다. EVM(Embedded Virtual Machine)은 ANSI C 언어와 SUN사의 Java 언어 등을 모두 수용할 수 있는 임베디드 시스템을 위한 가상 기계이며, SIL(Standard Intermediate Language)은 EVM에서 실행되는 중간언어로 다양한 프로그래밍 언어를 수용하기 위해서 객체지향 언어와 순차적 언어를 모두 수용하기 위한 연산 코드 집합을 갖고 있다. EVM을 위한 ANSI C 컴파일러는 ANSI C 언어를 받아 들여 EVM의 중간 언어인 SIL 코드를 출력한다. ANSI C 컴파일러에서 어휘 분석과 구문 분석 과정에서 인식되는 명칭에 대해서 그 속성들을 수집하고 이용한다. 이 속성들은 명칭이 명시적으로 혹은 묵시적으로 정의되는 곳에서 심벌 테이블에 수집된다. 본 논문에서는 수집된 정보가 올바르게 되었는지 확인하기 위하여 심벌 테이블에 있는 정보를 다시 ANSI C 언어로 복원시키는 역변역기(detranslator)를 구현하였다.

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Opportunistic Interference Management for Interfering Multiple-Access Channels (간섭 다중 접속 채널에서의 기회적 간섭 관리 기술)

  • Shin, Won-Yong;Park, Dohyung
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.37B no.10
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    • pp.929-937
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    • 2012
  • In this paper, we introduce three types of opportunistic interference management strategies in multi-cell uplink networks with time-invariant channel coefficients. First, we propose two types of opportunistic interference mitigation techniques, where each base station (BS) opportunistically selects a set of users who generate the minimum interference to the other BSs, and then their performance is analyzed in terms of degrees-of-freedom (DoF). Second, we propose a distributed opportunistic scheduling, where each BS opportunistically select a user using a scheduler designed based on two threshold, and then its performance is analyzed in terms of throughput scaling law. Finally, numerical evaluation is performed to verify our result.

Development of English Stress and Intonation Training System and Program for the Korean Learners of English Using Personal Computer (P.C.) (퍼스컴을 이용한 영어 강세 및 억양 교육 프로그램의 개발 연구)

  • Jeon, B.M.;Pae, D.B.;Lee, C.H.;Yu, C.K.
    • Speech Sciences
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    • v.5 no.2
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    • pp.57-75
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    • 1999
  • The purpose of this paper is to develop an English prosody training system using PC for Korean learners of English. The program is called Intonation Training Tool (ITT). It operates on DOS 5.0. The hardware for this program requires over IBM PC 386 with 4 MBytes main memory, SVGA (1 MByte or more) for graphic, soundblaster 16 and over 14 inch monitor size. The ITT program operates this way: the learners can listen as well as see the English teacher's stress and intonation patterns on the monitor. The learner practices the same patterns with a microphone. This program facilitates the learner's stress and intonation patterns to overlap the teacher's patterns. The learner can find his/her stress and intonation errors and correct these independently. This program is expected to be a highly efficient learning tool for Korean learners of English in their English prosody training in the English class without the aid of a native English speaker in the classroom.

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The Potentiometric Performance of Mercury (II) Ion-Selective Electrode Based on Tetracycline Antibiotics

  • Baek, Jong-Gyu;Rhee Paeng, In-Sook
    • Journal of the Korean Electrochemical Society
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    • v.11 no.1
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    • pp.59-63
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    • 2008
  • Poly (vinylchloride) (PVC) membrane electrodes based on neutral carrier, tetracycline was prepared as an active sensor for Hg(II) ion, and tested in different contents of the potassium tetrakis (4-chlorophenyl) borate (KTpClPB) as lipophilic salt. Bis (2-ethylhexyl) sebacate (DOS), bis(l-butylpentyl) adipate (BBPA), 2-nitrophenyl octyl ether (NPOE) and dibutyl phthalate (DBP) were used as diverse plasticizing solvent mediators. This electrode shows excellent potentiometric response characteristics and display good linearity with log $[Hg^{+2}]$ versus EMF response, over a range of concentrations between $10^{-7}$ and $10^{-3}M$. With 30.8mV/decade Nernstian slope, the detection limit was $6.9{\times}10^{-9}M$ and the response time was less than 20s. The proposed electrode yields very good selectivity for mercury (II) ion over many cations such as alkali, alkaline earth, transition and heavy metal ions. And it shows a very stable potential values in a wide pH range. This reliable electrode prepared was kept at least a month without considerable alteration in their response to Hg (II) ion.

Atmospheric Correction Problems with Multi-Temporal High Spatial Resolution Images from Different Satellite Sensors

  • Lee, Hwa-Seon;Lee, Kyu-Sung
    • Korean Journal of Remote Sensing
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    • v.31 no.4
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    • pp.321-330
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    • 2015
  • Atmospheric correction is an essential part in time-series analysis on biophysical parameters of surface features. In this study, we tried to examine possible problems in atmospheric correction of multitemporal High Spatial Resolution (HSR) images obtained from two different sensor systems. Three KOMPSAT-2 and two IKONOS-2 multispectral images were used. Three atmospheric correction methods were applied to derive surface reflectance: (1) Radiative Transfer (RT) - based absolute atmospheric correction method, (2) the Dark Object Subtraction (DOS) method, and (3) the Cosine Of the Uun zeniTh angle (COST) method. Atmospheric correction results were evaluated by comparing spectral reflectance values extracted from invariant targets and vegetation cover types. In overall, multi-temporal reflectance from five images obtained from January to December did not show consistent pattern in invariant targets and did not follow a typical profile of vegetation growth in forests and rice field. The multi-temporal reflectance values were different by sensor type and atmospheric correction methods. The inconsistent atmospheric correction results from these multi-temporal HSR images may be explained by several factors including unstable radiometric calibration coefficients for each sensor and wide range of sun and sensor geometry with the off-nadir viewing HSR images.

An Effective Orientation-based Method and Parameter Space Discretization for Defined Object Segmentation

  • Nguyen, Huy Hoang;Lee, GueeSang;Kim, SooHyung;Yang, HyungJeong
    • KSII Transactions on Internet and Information Systems (TIIS)
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    • v.7 no.12
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    • pp.3180-3199
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    • 2013
  • While non-predefined object segmentation (NDOS) distinguishes an arbitrary self-assumed object from its background, predefined object segmentation (DOS) pre-specifies the target object. In this paper, a new and novel method to segment predefined objects is presented, by globally optimizing an orientation-based objective function that measures the fitness of the object boundary, in a discretized parameter space. A specific object is explicitly described by normalized discrete sets of boundary points and corresponding normal vectors with respect to its plane shape. The orientation factor provides robust distinctness for target objects. By considering the order of transformation elements, and their dependency on the derived over-segmentation outcome, the domain of translations and scales is efficiently discretized. A branch and bound algorithm is used to determine the transformation parameters of a shape model corresponding to a target object in an image. The results tested on the PASCAL dataset show a considerable achievement in solving complex backgrounds and unclear boundary images.