• 한국임상수의학회지
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• 제19권3호
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• pp.328-332
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• 2002

Cordyceps is reputed for its broad biological activities and as a tonic for replenishing vital function in Chinese traditional medicines. As an attempt to obtain fundamental data for the development of new type Cordyceps, the effects of the fruiting bodies of cultivated fungus of Paecilomyces japonica grown on silkworm larvae on fatigue and hematotoxicity were investigated. The antifatigue activity of dongchongxiacao (Paecilomyces japonica) was studied in mice using weight loaded forced swimming performance method. The water extract of dongchongxiacao(25 mg/kg of body weight, intraperitoneal injection at 12 and 36 hours before irradiation and 30 minute and 24 hours after irradiation) showed a significant antifatigue effect in normal mice and radiation-induced fatigue mice (p<0.0005). In the experiment on the improvement of hematotoxicity, a significant difference from the irradiated controls was seen at day 3 of the group treated with dongchongxiacao and substantial differences from the irradiated controls at day 7 was also detected.

• 대한수의학회지
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• 제43권2호
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• pp.181-188
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• 2003

Cordyceps has a reputation for its broad biological activities and as a tonic which replenishs vital function in Chinese traditional medicines. As an attempt to obtain fundamental data for the development of new type Cordyceps, the effects of the fruiting bodies of cultivated fungus of Paecilomyces japonica grown on silkworm larvae on radiationinduced damages were investigated. We performed this study to determine the effect of Dongchongxiacao (Paecilomyces japonica) on jejunal crypt survival, endogenous spleen colony formation, and apoptosis in jejunal crypt cells and hair follicles of mice irradiated with high and low dose of gamma-radiation. Treatment with Dongchongxiacao showed no significant modifying effects on the jejunal crypt survival and endogenous spleen colony formation. The frequency of radiationinduced apoptosis was reduced by pretreatment of Dongchongxiacao (i.p.: 50 mg/kg of body weight, at 12 and 36 hours before irradiation, p<0.01). The spontaneous levels of apoptotic cells are $0.082{\pm}0.041$ in intestinal crypts and $0.231{\pm}0.084$ per hair follicle section of skin. Pretreatment of Dongchongxiacao was associated with decreases of 26.86% in intestinal crypt and 66.36% in hair follicle decrease in the number of cells with nuclei positively stained for apoptosis compared with the irradiation control group. We demonstrated for the first time that Dongchongxiacao administration could reduce the extent of apoptosis produced by radiation in the hair follicle. The results presented herein that Dongchongxiacao given before irradiation is capable of reducing the severity of cell loss as a result of apoptosis.

• Journal of Radiation Protection and Research
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• 제37권1호
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• pp.50-55
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• 2012

자외선에 의한 만성 피부손상에 대한 누에동충하초($Paecilomyces$ $japonica$)추출물(PJE)의 방어효과를 피부주름 및 암발생을 지표로 관찰하였다. 자외선 단독 조사마우스에서 피부노화의 지표인 피부주름이 형성되었으며, PJE투여에 따라 주름등급은 낮게 나타났다. 실험 22주에 자외선 단독조사군에서 부형제 복강내주사군은 88.9%, 연고기재 피부도포군에서는 44.4%의 동물에서 1개 이상의 피부암이 발생되었다. PJE 복강내 주사의 경우 암발생 개시시간이 지연되었으며, 발생율은 PJE 복강내 주사군 및 피부도포군에서 공히 감소하였고, PJE 복강내 주사군의 경우 마리당 발생암의 수도 감소하였다. 실험 22주에 PJE 복강내 주사군에서는 80.0%, 피부도포군에서는 75.0%의 동물에서 암은 발생되지 않았다. 결론적으로 자외선 만성 노출에 의해 피부의 광노화 및 광발암이 나타났고 PJE에 의해 피부만성 손상이 완화됨을 알 수 있었다.

• Journal of Radiation Protection and Research
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• 제26권1호
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• pp.27-33
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• 2001

Apoptosis의 유발을 억제하는 한약제를 파악하여 apoptosis와 관련된 정상적 또는 질병에 대한 연구에 자료를 제공하고자 방사선에 의해 유도된 apoptosis를 지표로 한의학적 처방에서 많이 사용되는 대표적 한약제(24종)의 효과를 검증하였다. 용안육, 산조인, 원지, 인삼, 복령 목향, 천궁 백작약, 승마, 시호 및 눈꽃동충하초 투여군에서 apoptosis는 감소되었으며, 이들 생약제는 apoptosis와 관련된 질병의 예방 및 치료에 적용할 수 있을 것이다. 추후 이들 생약의 작용 기전에 대한 연구가 요구된다.

• International Journal of Industrial Entomology and Biomaterials
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• 제20권1호
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• pp.29-35
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• 2010

To compare the quality of manufactured goods distributed in the domestic markets, 6 isolates of Dongchongxiacao products, namely, 4 Paecilomyces tenuipes specimens (J2P, 901A, 901B, and 901C), 1 Cordyceps militaris specimen (901D), and 1 Cordyceps sinensis specimen (CI-1), were analyzed for their physicochemical properties and sequence-structure relationships. P. tenuipes (J2P), a kind of Dongchongxiacao, was successfully inoculated on silkworms by percutaneous infection of Rural Development AdminstraionNam et al., 1999); fruiting bodies were then formed on the complete surface of the pupa. Since P. tenuipes (J2P) from silkworm larva was also proved to have remarkable pharmacological activities, it has been produced in bulk and has been successfully sold to buyers in the Korean market. Additionally, imitation products such as 901A, 901B, 901C, 901D, and CI-1 were sold simultaneously, resulting in deterioration of product quality. This research focuses on establishing quality standards to discriminate between the original and imitation products circulating in the market. The products obtained for the experiments included J2P, 901A, 901B, 901C, 901D, and CI-1; proximate analysis was performed for these products. The hosts and methods of conidia inoculation for proliferation of mycelia differed among the products. P. tenuipes (J2P) was proliferated in live silkworm larvae, and dead silkworm pupae were used to produce 901A, 901B, and 901C. On the other hand, 901D was produced on hulled rice medium. Quality analysis of C. sinensis revealed that CI-1, which was imported from China, smelled bad and proved to be a counterfeit with the fruiting body glued to the insect by twigs. The results of the proximate analysis of 901A, 901B, 901C, and 901D were similar to those of J2P with respect to the moisture content. Otherwise, J2P contained higher crude protein than 901A, 901B, 901C, and 901D, but contained very low fat. C. militaris (901D) and C. sinensis (CI-1) had low crude protein content-12.79% and 9.78% respectively-as compared to that of J2P, which was 62.38%. In contrast to the crude ash content of 6.4% in J2P, the crude ash content of CI-1 was 18.51% and this specimen was found to contain many impurities. phylogenetic analysis of P. tenuipes revealed that the sequence similarity of J2P, 901B, and 901C was in the range of 92.3~92.7%. Additionally, differences in the sequences were found at the positions 65 bp, 436 bp, 441 bp, 463 bp, etc.