• 미생물학회지
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• 제35권4호
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• pp.307-314
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• 1999

본 연구는 동치미에서 유산균을 분리하여 동치미의 가능성이 강화된 무 주스를 제조하기 위한 starter로써의 가능성을 검토하기 위하여 유산균의 발효특성을 조사하였다. 동치미에서 분리 동정된 40 균주는 당 발효능과 양상에 따라 5개의 group으로 분류되었다. Species별 분포를 살펴본 결과는 Leuconostoc mesenteroides, Lactobacillus brevis, Lactobacillus sake, Lactobacillus plantarum, Lactobacillus fermentum의 5개 균종이었으며, 유산균의 분포는 Leuconostoc mesenteroides가 52.5%로 최 우점종으로 나타났다. 분리된 5개의 group에서 가장 생육능이 왕성한 1개 균주씩 Leuconostoc mesenteroides J-9, Lactobacillus brevis J-12, Lactobacillus plantarum J-39, Lactobacillus fermentum J-7 등 5균주를 선정하여 한외 여과한 무즙에 각각 0.3($10^6$ cfu/㎖)%씩 접종하여 25${\circ}C$에서 9일간 숙성하면서 pH 및 총 산도, 비휘발성유기산함량의 변화 등을 조사하였다. 각 접종 균주 중 Lactobacilus plantarum의 생육능이 가장 빠르게 나타났으며 Lactobacillus sake가 가장 느리게 성장하였다. 발효 개시 전의 한외 여과한 무즙의 pH 및 총 산도는 6.30~6.36, 0.09~1.0%이었으나 9일째 pH 3.2~4.3, 0.85~1.2%로 나타났다. 각 접종 균주의 pH, 산도의 변화를 비교해보면 미생물의 생육능과 상관성이 높아 유산균수의 증가속도가 빠를수록 pH는 빠르게 감소하고, 산도는 빠르게 증가하였다. 비휘발성 유기산의 변화를 보면 모든 접종구에서 시트르산, 말산, 말론산, 숙신산은 감소하는 경향으로 나타났으며 락트산은 발효가 진행됨에 따라 증가하였다. 관능적으로 맛에 좋은 영향을 주는 유기산으로 알려진 숙신산과 락트산의 함량을 보면 L. mesenteroides J-9가 가장 높게 나타났다. 락트산 단독의 경우는 L. plantarum J-39m, L. brevis J-12 접종구의 순으로 높게 나타났다. 이 ${\cdot}$ 화학적 검사와 향미에 대한 관능검사결과 L. mesenteroides J-9, L. brevis J-12, L. fermentum J-7이 무쥬스의 젖산발효를 위한 starter로써 적합한 것으로 나타났다.

• 한국식품위생안전성학회지
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• 제22권3호
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• pp.151-158
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• 2007

동치미로부터 분리한 유산균 (68 균주) 중 Escherichia coli O157에 대한 항균 효과를 나타내는 균주는 Lactobacillus plantarum K11로 동정되었다. 분리균주 La. piantarum K11이 생산한 박테리오신의 항균 활성은 대수증식기 후반부에 12,800 BU/mL로 최대 활성에 이르렀다. 항균 활성은 pepsin, protease, proteinase K, papain, chymotrypsin 및 trypsin 처리에 의해 완전히 소실되었으나, catalase, ${\alpha}-amylase$, lysozyme 및 lipase에 의해서는 영향을 받지 않았으므로 단백질성 물질임을 확인하였다. 게다가, 이 활성은 pH 3.0-9.0의 조건하에서나 -20, 4 및 $25^{\circ}C$에서 30일간의 저장 동안에도 안정하였다. 또한 $100^{\circ}C$에서 30분간 가열처리에도 비교적 안정한 편이었고, chloroform이나 hexane 처리에도 활성에 변함이 없었다. 분리 균주의 박테리오신은 Bacillus spp., Listeria spp. 및 Staphylococcus spp. 등의 일부 식중독균의 억제효과는 나타나지 않았으나, Enterobacter aerogenes와 E. coli 등의 장내세균의 억제에는 효과적이었으며, 특히 640 BU/mL의 박테리오신 처리에 의해서 10시간 배양만에 E. coli O157이 완전하게 사멸되었다.

• 한국식생활문화학회지
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• 제6권2호
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• pp.155-173
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• 1991

This study was conducted to research on the observing state of Korean traditional holidays and the traditional foods related to those days through the questionnaires completed by 578 housewives who live in Kangreung, Daejeon, Jeonju, and Daegu area. For data processing, SAS program was employed, and all data was analyzed by frequency, percentage, and $x^2-square$. The results are as follows; 1. In observing the traditional holidays, more than 90% of housewives who answered the inquiry keep up SEOLNAL, DAEBOREUM, and CHUSEOK, whithout regarding regions. There are larger number of people who keep up IPCHUN, JUNGWHAJEAL, and DANOH in Kangreung and SAMBOK in Daegu than that of other regions (p<.001). Compared to general circumstances, CHOPAIL is more commonly celebrated by older class as well as lower educated class (p<.001) Also, the consequence shows that Buddhists observe IPCHUN, SAMJITANL, CHOPAIL, DANOH, SAMBOK, and DONGJI more commonly compared with other classes (p<.001), IPCHUN, SAMJITNAL, (p<.01). DAEBOREUM, YUDU, SAMBOK, JUNGYANGJEOL, OHIL, and DONGJI (p<.001) are celebrated more generally among extended. 2. On inheritance of the traditional holidays, most of the housewives answered that the SEOLNAL, DAEBOREUM, CHUSSEOK, and DONGJI are to be inherited. Compared to general circumstance CHOPAIL is suggested to be inherited by older people (p<.001). The IPCHUN, CHOPAIL, SAMBOK (p<.001), and DANCH (p<.01) are suggested by Buddhists that YUDU and SAMBOK are by nuclear. 3. In preparaing festive foods, DEOKGUK on SEOLNAL, OHKOKBAP and MUKEUNNAMUL on DAEBOREUM, SONGPYEON and GATEUNNAMUL on CHUSEOK, and PATJUK on DONGJI are being made very generally. KANGJEONG on SEOLNAL, BUREUM on DAEBOREUM, KALGUKSU and SAMGYAETANG on SAMBOK, DARKJIM on CHUSEOK, SUJEONGGWA and DONGCHIMI on DONGJI appeared comparatively high rated of making. 4. In normal times, DEOKGUK, MANDU, INJEOLMI, SIKHYAE, SUJEEONGGWA, PYEONYUK, JEONYUEO, SIRUDDEOK, BOKSSAM, MUKEUNNAMUL, SONGPYUN, MINARINAMUL, YUKGAEJANGGUK, KALGUKSU, SAMGYAETANG, HOBAKJIJIM, TORANTANG, GATEUNNAMUL, NUREUMJEOK, DAKJIM, KALBIJIM, PATJJUK, and DONGCHIMI is usully made. 5. The source to learn about traditional foods is mostly by her mother and the rest orders are husband's mother, cooking books, mass media (including T.V), school education, and cooking instituse, etc.

• 생명과학회지
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• 제11권5호
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• pp.439-446
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• 2001

동치미에서 분리한 Leu. mesenteroides subsp. cremoris DLAB19 균주의 MNNG와 4-NQO에 대한 항돌연변이원성물질 생산을 위한 최적 조건을 조사한 결과, 탄소원으로 glucos들 첨가시 가장 높은 항돌연변이 효과를 나타내었으며, 질소원으로서 yeast extract와 bactopeptone 첨가시 항돌연변이 효과가 우수하였다. 탄소원으로 glucose의 농도를 2% 첨가시 MNNG와 4-NQO에 대한 항돌연변이 효과가 가장 우수하였으며, 질소원으로서 yeast extract와 bac-topetone의 농도를 1% 첨가시 가장 우수한 항돌연변이효과를 나타내었다. 항돌연변이원성 물질 생산을 위한 최적 배양 조건은 pH, 배양 온도, 배양 속도가 각각 7.0, 3$0^{\circ}C$ 및 150 rpm이었다. 상기의 최적 조건에서 36시간 배양시 가장 높은 항돌연변이 효과를 나타내었는데 S. enterica serovar typhimurium TA100과 TA98을 이용한 겨우 항돌연변이 효과가 각각 96.4%와 53.8%이었다.

• 한국식품과학회지
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• 제34권3호
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• pp.472-479
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• 2002

식중독 미생물의 증식을 억제하며, probiotic으로 작용할 수 있는 균주로서 가능성이 있는 젖산균을 신생아 분변과 동치미서 젖산균을 분리(0.15%의 담즙산염과 pH 3.0인 산성조건)하였고, 항균활성이 우수한 젖산균을 선발하였다. D2와 F35-2 균주는 Lactobacillus plantarum, F20-3 균주는 L. fermentum으로 동정되었다. 젖산균 대사산물의 특성과 항균 기작을 알아보기 위해 몇 종의 식중독 미생물(Listeria monocytogenes, Bacillus cereus, Staphylococcus aureus, Salmonlla Typhimurium, Escherichia, coli O157:H7, Salmonella Enteritdis)을 사용하였으며, 항균활성 Bioscreen C로 측정하였다. 젖산균의 대사산물의 특성을 확인한 결과 pH가 중성으로 갈수록 항균효과가 감소하는 경향을 나타내었다. Catalase 효소처리 후에는 F20-3 균주만 항균활성이 소실되어 항균활성의 원인으로 $H_2O_2$의 영향이 있을 것으로 추정되었다. 단백질 분해 효소(trypsin, pepsin)처리에는 항균활성에 영향이 없었고, $121^{\circ}C$ 15분간 열처리에도 안정한 물질로 추정되었다. 분리균주 대사산물은 유사한 pH와 젖산 농도보다 식중독 미생물의 증식 억제가 우수하여 분리된 균주의 증식억제 물질은 유기산 및 대사산물과 관계가 있을 것으로 추정되었다. 분리균주가 생성하는 유기산을 HPLC로 정량 한 결과, D2와 F35-2는 발효 24시간에 각각 1.84, 1.85%의 젖산만이 생성되었고, F20-3은 발효 24시간에 0.91%의 젖산과 0.22%의 초산을 생성하였다. 이것으로 볼 때 젖산발효형태는 D2와 F35-2는 homo형이고, F20-3은 hetero형의 발효를 하는 것으로 확인되었다.

• 한국식품조리과학회지
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• 제18권6호
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• pp.684-691
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• 2002

The objectives of this study were to determine the consumption pattern and importance of Kimchi in our meals, and the preference of Kimchi. The 77.0% of the housewife respondents were preparing Kimchi at home. The reasons for not making Kimchi were ‘don't have time’(39.4%), ‘don't know how to make Kimchi’(18.2%) and ‘don't eat much’(18.2%). The amounts of daily Kimchi intake of the respondents were ‘less than 50g’(42.3%) or ‘50-100g’(29.8%). They are Kimchi because it was ‘delicious’(48.4%) or ‘habitual’(39.5%). The 70.0% of the respondents answered that Kimchi was important in each meal. The most respondents favored ‘moderate’ in the degree of fermentation(48.1%), hot taste(65.0%) and salty taste(72.4%). As the age of the respondents increased, they favored less sour, less hot and less salty taste. Baechu Kimchi(4.15), Chonggak Kimchi(3.89), Dongchimi(3.85), Kkaktugi(3.74), water Kimchi(3.67) were preferred by the respondents. The 73.97% of the respondents preferred Kimchi Jjigye. The 10- to 19-years old respondents preferred Kimchi Jjigye(52.8%), Kimchi pan cake(24.5%) and fried rice with Kimchi(17.0%).

• Journal of Microbiology and Biotechnology
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• 제19권2호
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• pp.178-186
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• 2009

To examine their potential as probiotics, acid and bile tolerance, antibiotics resistance, adhesion capacity to Caco-2 and HT-29, and antibacterial activity, of LAB isolated from Korean fermented foods such. as dongchimi, kimchi, Meju, and doenjang were assayed against foodborne pathogenic bacteria. DC 55, DC 136, DC 222, KC 21, KC 24, KC 34, KC 43, KC 117, MJ 54, MJ 301, SP 33, and SP 170 strains were resistant to acid and bile conditions. In particular, DC 55, DC 136, KC 24, KC 43, and MJ 301 strains were highly resistant to higher than 20 ${\mu}g/ml$ concentrations of vancomycin, streptomycin sulfate, or amoxicillin, whereas, DC 222, KC 21, KC 34, KC 117, MJ 54, and SP 33 strains were susceptible to lower than 2 ${\mu}g/ml$ concentrations of those antibiotics. The adhesion to HT-29 and Caco-2 cells varied with the strains tested in a strain-dependent manner. The highest level of adhesion was observed with DC 55, KC 21, KC 24, and MJ 301 strains, having higher than 50% of adhesion to HT-29 or Caco-2 cells. In addition, Staphylococcus aureus was the most sensitive to KC 21, showing an inhibition of about 70%, and the antibacterial activity of KC 21 against S. aureus resulted most likely from both organic acids and bacteriocin. Based on its phenotypic characteristics and utilization of various sugars, the KC 21 strain was identified as Lactobacillus plantarum.

• 한국조리학회지
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• 제13권2호
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• pp.59-68
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• 2007

This research was to investigate the consumers' perception and purchase behavior of Korean rice cake as a meal. The rate of consumers who had eaten rice cake as a meal was 64.7% and that of those who had not was 35.3%.. For breakfast, 49.83% of them ate rice cake and for lunch 21.2% of them did. Reasons for eating rice cake for meal were investigated on 'easy to eat(63.21%), 'the others(13.0%)', 'good taste (12.3%)' and 'healthy food(10.8%)'. Reasons for not eating rice cake for meal were researched on 'snack(40.09%), 'difficult storage(21.9%)' and 'expensive(9.7%)'. After purchasing or making rice cake, 45.4% of consumers ate immediately and 30.9% of them ate within $1{\sim}2\;days$. In thawing methods of frozen rice cake as a meal, 51.3% of consumers used a microwave. Kinds of beverage with which rice cake was eaten were water(35.3%), coffee(14.9%), milk(13.8%) and Kimchi(dongchimi, 13.8%). The complementary points for purchase of rice cake as a meal were 'Small package(23.2%)', 'Good preference'(20.5%), 'Healthy food(15.0%)', 'Affordable price(12.7%)' and 'Low calorie(9.5%). Kinds of rice cake as a meal were Injeolmi(16.0%), Backsulgi(15.4%), Yaksik(9.3%) and Galaetteok(9.0%).

• 한국식생활문화학회지
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• 제22권1호
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• pp.10-21
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• 2007

The purpose of this study was to investigate the housewives’ consumption pattern and preference of the Korean rice cake asa substitute for meal. The rate of housewives who had eaten rice cake as a substitute for meal were 75.30% and those who had not were 20.83%. Usually 62.03% of them ate rice cake for breakfast, and 34.21% did them within one time per a week. Reasons for eating rice cake for meal were investigated on ‘easy to eat(54.51%)’, ‘good taste (24.44%)’ and ‘healthy food(14.29%)’. The older housewives wanted healthy rice cake for meal and ate with tea and Kimchi(Dongchimi). The younger ones ate rice cake for meal with tea and milk. Thawing methods of frozen rice cake for meal were different between the older and the younger, respectively, steaming and using microwave. Kinds of rice cake as a substitute meal were Injeolmi(50.75%), Backsulgi(49.62%), Chaltteok (47.74%) and Yaksik(46.44).

• 한국식품영양학회지
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• 제28권5호
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• pp.894-909
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• 2015

The purpose of this research aims to reveal how radish kimchi (Jong-ga) differs in Jong-ga recipes and old cookbooks. To accomplish this, old cookbooks ("Soowoonjabbang", "Jusiksiui", "Eumsikbangmunnira", "Siuejunsuh", "Banchandeungsok", and "Buinpilji") were reviewed and 8 Jong-ga recipes (Seogye Park Se-dang from the Bannam Park clan Jong-ga, Myungsukgong from the Changnyeong Jo clan Jong-ga, Nampa Park Jae-gyu from the Milyang Park clan Jong-ga, Geunggudang Kim Joong-jeung from the Gwangsan Kim clan Jong-ga, Dongchundang Song Jun-gil from the Eunjin Song clan Jong-ga, Myeongjae Yun Jung from the Papyung Yun clan Jong-ga, Daeseunggong Ryu Cha-dal from the Munhwa Ryu clan Jong-ga, Inmukjae Son Sung-jeung from the Milseong Son clan Jong-ga) from five areas were reviewed. We classified the radish kimchi into five categories, radish kimchi, Dongchimi, kkakdugi, Seokbakji and Nabak kimchi and other kimchi. According to old cookbooks, most kimchi was made with radish, cabbage, cucumber, pear, yuju, fish meat, and salt. Modern Jong-ga is made of seasoned radish, sticky rice paste, seafood, sugar, powdered pepper, fish sauce and salt. This study helps to understand notable clans' cultures via their recipes for kimchi.