• 한국식품저장유통학회지
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• 제14권6호
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• pp.681-687
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• 2007

동충하초 20여종의 균사와 배양 배지를 이용하여 생물학적 활성 효과를 항산화와 항암 실험을 통하여 알아보았다. 항산화 활성 실험 DPPH와 FRAP실험을 실시하였는데 다수의 동충하초 추출물에서 항산화 활성을 확인할 수 있었으며, 시간에 따른 FRAP활성에서는 배양배지에서 균사체 추출물보다 높은 활성을 나타내었다. NOS와 관련성을 확인하기 위하여 NO활성 실험을 한 결과 NO활성은 추출물의 종류에 따라 증가 또는 감소시키는 것을 알 수 있었다. 염증과의 관련성을 확인하기 위하여 Cox-2 promoter활성실험을 한 결과, 종에 따라 활성의 증가 또는 감소는 다양하게 나타났다. Wound healing assay를 이용하여 항암효과를 알아본 결과 많은 종에서 세포의 운동성을 억제하는 효과를 가지는 것으로 나타났다. 또한 혈관생성 억제효과를 알아보기 위하여 HUVECs를 이용하여 tube formation을 확인해 본 결과 다수의 추출물에서 혈관생성을 억제하는 것으로 나타났다.

• Mycobiology
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• 제45권1호
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• pp.31-38
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• 2017

Cordyceps militaris, known as Dong-Chong-Xia-Cao, produces the most cordycepin among Cordyceps species and can be cultured artificially. For these reasons, C. militaris is widely used as herb or functional food in the East Asia. In this study, we developed a new strain of C. militaris that produces higher cordycepin content than parent strains through mating-based sexual reproduction. Twenty parent strains were collected and identified as C. militaris based on internal trasncrived spacer and rDNA sequences. Seven single spores of MAT 1-1 idiomorph and five single spores of MAT 1-2 idiomorph were isolated from 12 parent strains. When 35 combinations were mated on the brown rice medium with the isolated single spores, eight combinations formed a stroma with a normal perithecia and confirmed mated strains. High pressure liquid chromatography analysis showed that mated strain KSP8 produced the most cordycepin in all the media among all the tested strains. This result showed due to genetic recombination occurring during the sexual reproduction of C. militaris. The development of C. militaris strain with increased cordycepin content by this approach can help not only to generate new C. militaris strains, but also to contribute to the health food or medicine industry.

• 한국생명과학회:학술대회논문집
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• 한국생명과학회 2006년도 제47회 학술심포지움 및 추계국제학술대회
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• pp.102.1-102.1
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• 2006

• International Journal of Industrial Entomology and Biomaterials
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• 제18권1호
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• pp.13-17
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• 2009

The caterpillar-shaped Chinese medicinal mushroom (DongChongXiaCao) looks like a worm in the winter and like a grass in the summer. The fruiting body has been regarded as popular folk or effective medicines used to treat human diseases such as asthma, bronchial and lung inflammation, and kidney disease. The fruiting bodies of Cordyceps militaris, C. pruinosa and Paecilomyces tenuipes that formed on the living silkworm (Bombyx mori) host were used in this examination. This study was carried out to investigate the soluble sugar, amino acid and fatty acid profiles in the fruiting-bodies. Soluble sugars such as glycerol, glucose, mannitol and sucrose were mainly found in the fruiting bodies of C. militaris, C. pruinosa and P. tenuipes. Total soluble sugar content was 29.23 mg/g in C. militaris, 8.61 mg/g in C. pruinosa and 24.00 mg/g 1in P. tenuipes on dry weight basis. Total free amino acid content was 14.09 mg/g 1in C. militaris, 34.60 mg/g in C. pruinosa and 17.09 mg/g in P. tenuipes. The content of oleic acid in fatty acids was above high more than 30% regardless of species.

• Mycobiology
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• 제35권4호
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• pp.215-218
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• 2007

The caterpillar-shaped Chinese medicinal mushroom (DongChongXiaCao) looks like a worm in the winter and like a grass in the summer. The fruiting body has been regarded as popular folk or effective medicines used to treat human diseases such as asthma, bronchial and lung inflammation, and kidney disease. The fruiting bodies of Paecilomyces tenuipes that formed on the living silkworm (Bombyx mon) host were used in this examination. This study was carried out to investigate the proximate composition, soluble sugar, amino acid and fatty acid profiles, and contents of the bioactive ingredient including adenosine and D-mannitol in the fruiting-bodies. The moisture content was 57.56%. Soluble sugars found were glycerol, glucose, mannitol and sucrose, and the contents exceeded $24\;mgg^{-1}dry$ weight. Total free amino acid content was $17.09\;mgg^{-1}dry$ weight. Arginine, glycine, proline and tyrosine were main amino acids. The content of oleic acid in fatty acids was high. Adenosine was more abundant in fruiting bodies than corpus.