Browse > Article

Comparison of Antioxidant, Anticancer and Immunomodulating Activities of Extracts from DongChongXiaCao  

Heo, Jin-Chul (Department of Food Science & Technology, Kyungpook National University)
Nam, Sung-Hee (Department of Agricutural Biology, National Advanced Institute of Science & Technology, RDA)
Kang, Seok-Woo (Department of Agricutural Biology, National Advanced Institute of Science & Technology, RDA)
Hong, In-Pyo (Department of Agricutural Biology, National Advanced Institute of Science & Technology, RDA)
Lee, Kwang-Kil (Department of Agricutural Biology, National Advanced Institute of Science & Technology, RDA)
Park, Ja-Young (Daegu Research Institute of Public Health & Environment)
Kim, Kyung-Hae (Department of Food Science & Technology, Kyungpook National University)
Han, Song-Yi (Department of Food Science & Technology, Kyungpook National University)
Lee, Sang-Han (Department of Food Science & Technology, Kyungpook National University)
Publication Information
Food Science and Preservation / v.14, no.6, 2007 , pp. 681-687 More about this Journal
Abstract
Biological properties of DongChongXiaCao extracts and culture supernatants were evaluated using DPPH and FRAP (antioxidants), Raw 264.7 (NO production), B16-F1 cells (cell migration activity) and HUVECs (angiogenesis activity). We found that antioxidant activity was higher in mycelium culture supernatants than in mycelial extracts. Mycelial extracts and culture supernatants inhibited or increased cyclooxygenase-2 transcription activity and NO production. Various extracts and culture supernatants inhibited B16 cell migration and motility, and inhibited HUVEC tube formation. These findings indicate that DongChongXiaCao extracts and products of mycelium could be a useful biological resource for anti-oxidant and anti-cancer purposes.
Keywords
DongChongXiaCao; anti-oxidant; anti-tumor; Cox-2; nitric oxide; angiogenesis; DPPH; FRAP;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Nadeem, A., Chhabra, S.K., Masood, A., and Raj, H.G. (2003) Increased oxidative stress and altered levels of antioxidants in asthma. J. Allergy Clin. Immunol., 111, 72-78   DOI   ScienceOn
2 Varshavskii, B., Trubnikov, G.V., Galaktipmpva, L.P., Koreniak, N.A., Koledeznaia, I. L. and Oberemok, A.N. (2003) Oxidant-antioxidant status of patients with bronchial asthma during inhalation and systemic glucocorticoid therapy. Ter. Arkh., 75, 21-24
3 Du, C., Guan, Q., Diao, H., Yin, Z. and Jevnikar, A.M. (2006) Nitric oxide induces apoptosis in renal tubular epithelial cells through activation of caspase-8. Am. J. Physiol. Renal Physiol., 290, 1044-1054   DOI
4 Dreher, D. and Junod, A.F. (1996) Role of oxygen free radicals in cancer development. Eur. J. Cancer, 32A, 30-38
5 Yoshitake, J., Akaike, T., Akuta, T., Tamura, F., Ogura, T., Esumi, H. and Maeda, H. (2004) Nitric oxide as an endogenous mutagen for Sendai virus without antiviral activity. J. Virol., 78, 8709-8719   DOI   ScienceOn
6 Heo, J.C., Park, J.Y., Lee, J.M., Kwon, T.K., Kim, S.U., Chung, S.K. and Lee, S.H. (2005) Wisteria floribunda gall extract inhibits cell migration in mouse B16F1 melanoma cells by regulating CD44 expression and GTP-RhoA activity. J. Ethnopharmacol., 102, 10-14   DOI   ScienceOn
7 Akaike, T., Okamoto, S., Sawa, T., Yoshitake, J., Tamura, F., Ichimori, K., Miyazaki, K., Sasamoto, K. and Maeda, H. (2003) 8-nitroguanosine formation in viral pneumonia and its implication for pathogenesis. Proc. Natl. Acad. Sci. U.S.A., 100, 685-690
8 Fang, J., Sawa, T. and Maeda, H. (2003a) Factors and mechanism of 'EPR' effect and the enhanced antitumor effects of macromolecular drugs including SMANCS. Adv. Exp. Med. Biol., 519, 29-49
9 MacNee, W. (2001) Oxidative stress and lung inflammation in airways disease. Eur. J. Pharmacol., 429, 195-207   DOI   ScienceOn
10 Rao, Y.K., Fang, S.H. and Tzeng, Y.M. (2007) Evaluation of the anti-inflammatory and anti-proliferation tumoral cells activities of Antrodia camphorata, Cordyceps sinensis, and Cinnamomum osmophloeum bark extracts. J. Ethnopharmacol., 114, 78-85   DOI   ScienceOn
11 Benzie, I.F. and Strain, J.J. (1996) The ferric reducing ability of plasma(FRAP) as a measure of 'antioxidant power': the FRAP assay. Anal. Biochem., 239, 70-76   DOI   ScienceOn
12 Droge, W. (2002) Free radicals in the physiological control of cell function. Physiol. Rev., 82, 47-95   DOI
13 van Wetering, S., van den Berk, N., van Buul, J.D., Mul, F.P., Lommerse, I., Mous, R., ten Klooster, J.P., Zwaginga, J.J. and Hordijk, P.L. (2003) VCAM-1- mediated Rac signaling controls endothelial cell-cell contacts and leukocyte transmigration. Am. J. Physiol. Cell Physiol., 285, 343-352   DOI
14 Kaltschmidt, B., Linker, R.A., Deng, J. and Kaltschmidt, C. (2002) Cyclooxygenase-2 is a neuronal target gene of NF-kappaB. BMC Mol. Biol., 4, 3-16
15 Del Rio, L.A., Corpas, F.J., Sandalio, L.M., Palma, J.M., Gomez, M. and Barroso, J.B. (2002) Reactive oxygen species, antioxidant systems and nitric oxide in peroxisomes. J. Exp. Bot., 53, 1255-1272   DOI   ScienceOn
16 Bussolati, B., Dunk, C., Grohman M., Kontos, C.D., Mason, J. and Ahmed, A. (2001) Vascular endothelial growth factor receptor-1 modulates vascular endothelial growth factor-mediated angiogenesis via nitric oxide. Am. J. Pathol., 159, 993-1008   DOI   ScienceOn
17 Nomura, T., Kikuchi, M., Kubodera, A. and Kawakami, Y. (1997) Proton-donative antioxidant activity of fucoxanthin with 1,1-diphenyl-2-picrylhydrazyl(DPPH). Biochem Mol. Biol. Int., 42, 361-370
18 Chung-man Ho, J., Zheng, S., Comhair, S.A.A., Farver, C. and Erzurum, S.C. (2001) Differential expression of manganese superoxide dismutase and catalase in lung cancer. Cancer Res., 61, 8578-8585
19 Al-Gayyar, M.M., Eissa, L.A., Rabie, A.M. and El-Gayar, A.M. (2007) Measurements of oxidative stress status and antioxidant activity in chronic leukaemia patients. J. Pharm. Pharmacol., 59, 409-417   DOI   ScienceOn
20 Huang, H., Wang, H. and Luo, R.C. (2007) Inhibitory effects of cordyceps extract on growth of colon cancer cells. Zhong Yao Cai., 30, 310-313
21 Lin, X.X., Xie, Q.M., Shen, W.H. and Chen, Y. (2001) Effects of fermented Cordyceps powder on pulmonary function in sensitized guinea pigs and airway inflammation in sensitized rats. Zhongguo Zhong Yao Za Zhi., 26, 622-625
22 Fang, J., Sawa, T., Akaike, T., Akuta, T., Sahoo, S.K, Greish, K., Hamada, A. and Maeda, H. (2003b) In vivo antitumor activity of pegylated zinc protoporphyrin: Targeted inhibition of heme oxygenase in solid tumor. Cancer Res., 63, 3567-3574
23 Sundaresan, M., Yu, Z.X., Ferrans, V.J., Irani, K. and Finkel, T. (1995) Requirement for generation of $H_{2}O_{2}$ for platelet-derived growth factor signal transduction. Science, 270, 296-299   DOI   ScienceOn
24 Fang, J., Nakamura, H. and Iyer, A.K. (2007) Tumor-targeted induction of oxystress for cancer therapy. J. Drug Target, 15, 475-486   DOI   ScienceOn
25 Afonso, V., Champy, R., Mitrovic, D., Collin, P. and Lomri, A. (2007) Reactive oxygen species and superoxide dismutases: role in joint diseases. Joint Bone Spine, 74, 324-329   DOI   ScienceOn
26 Lee, H.M., Kim, Y.J., Kim, H.W., Lee, D.H., Sung, M.K. and Park, T.S. (2006) Induction of apoptosis by Cordyceps militaris through activation of caspase-3 in leukemia HL-60 cells. Biol. Pharm. Bull., 29, 670-674   DOI   ScienceOn
27 Huang, P., Feng, L., Oldham, E.A., Keating, M.J. and Plunkett, W. (2000) Superoxide dismutase as a target for the selective killing of cancer cells. Nature, 407, 390-395   DOI   ScienceOn
28 Matsuyama, T., Ihaku, D., Tanimukai, T., Uyama, O. and Kitada, O. (1993) Superoxide dismutase suppressed asthmatic response with inhibition of manganese superoxide induction in rat lung. Nippon Kyobu Shikkan Gakkai Zasshi., 31, 139-145
29 Kuo, Y.C., Tsai, W.J., Wang, J.Y., Chang, S.C., Lin, C.Y. and Shiao, M.S. (2001) Regulation of bronchoalveolar lavage fluids cell function by the immunomodulatory agents from Cordyceps sinensis. Life Sci., 68, 1067-1082   DOI   ScienceOn
30 Fujii, S., Akaike, T. and Maeda, H. (1999) Role of nitric oxide in pathogenesis of herpes simplex virus encephalitis in rats. Virology, 256, 203-212   DOI   ScienceOn
31 Kuwahara, H., Kanazawa, A., Wakamatu, D., Morimura, S., Kida, K., Akaike, T. and Maeda, H. (2004) Antioxidative and antimutagenic activities of 4-vinyl-2,6- dimethoxyphe nol(canolol) isolated from canola oil. J. Agric. Food Chem., 52, 4380-4387   DOI   ScienceOn