• The Research Journal of the Costume Culture
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• v.13 no.4 s.57
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• pp.564-575
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• 2005

The purposes of this study were to investigate the effects of shopping orientation on fashion shopping mall environments and purchase intention in internet fashion shopping mall. Data were obtained from 423 internet fashion shopping mall consumers who have experiences of buying products or visiting to internet fashion shopping mall in Busan, and were analyzed using by factor analysis, Cronbach's alpha, cluster analysis, ANOVA and Duncan test. The results showed shopping orientation perceived by Internet fashion shopping mall consumers were consisted five factors: Brand loyalty orientation, Economical orientation, Fashion orientation, Time saving orientation and Internet shopping orientation. Internet fashion shopping mall environments were composed of Visual information, Loading speed, Space composition, Product assortment, Checkout service, and Help desk. Consumers were classified by the shopping orientation into the Economical shopper, Fashion/brand shopper, and Convenience shopper. Economical shopper and Fashion/brand shopper rise in the estimation of Visual information, Product assortment, and Checkout service of shopping mall environment. Additionally, they were likely to have more intentions to purchase than the other types of shoppers in internet fashion shopping mall.

• Journal of Sensor Science and Technology
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• v.30 no.2
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• pp.109-113
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• 2021

In this study, an automated culture media replacement system was developed to analyze changes in the contraction characteristics of cardiomyocytes according to the state of the culture media. For the long-term storage of culture media, a Peltier refrigerator with a temperature of 5 to 8℃ was provided and a pH of 7.4 was maintained. The cell culture media of the cardiomyocytes was continuously replaced using interlocking pumps at a flow rate of 0.83 μl/h. The cardiomyocytes in which the culture media was replaced automatically demonstrated lower heartbeats per minute compared to samples in which there was no replacement. However, these cardiomyocytes moved more uniformly and produced greater displacement in one heartbeat cycle. It was observed that the sarcomere length of the cardiomyocytes increased due to the automated culture media replacement system. These cardiomyocytes were found to demonstrate better maturation compared to the control group. The maturation of cardiomyocytes was verified through staining images. The proposed automated culture media replacement system generates a uniform heart rate and improvements in contraction force. Based on the study, patient-specific drug toxicity assessments can be conducted using differentiated cardiomyocytes in induced pluripotent stem cells.

• Proceedings of the Korean Society of Computer Information Conference
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• 2017.07a
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• pp.62-65
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• 2017

본 논문에서는 <오버워치>나 <리그 오브 레전드> 등의 이용자끼리 실력을 겨루는 형태의 경쟁적인 온라인 게임들에서 이용되어지고 있는 게임 핵의 종류와 사용 실태를 조사하고 이에 따른 개선 방안을 제시한다. 여러 가지 종류의 온라인 게임 핵에 대해서 알아보고 해당 핵이 어떤 게임에서 어떤 식으로 적용 되는지 설명하고, 유저들이 핵으로 인하여 개발사, 대회 주관사 등의 여러 기관에서 입는 피해 사례를 조사하고 이에 따른 현재 사용되고 있는 각 개발사들의 조치 방안 및 유저 커뮤니티의 의견 등을 통해 실태에 대해서 연구한다. 결론으로는 위의 조사한 자료를 바탕으로 어떻게 하면 현재 성행하고 있는 게임 핵사용을 방지하고 차단하는지에 대한 개선 방안을 제시하고 나아가 개발 및 운영 차원에서는 어떤 식으로 지원해야 하는지 등의 내용을 제시한다.

• Journal of Microbiology and Biotechnology
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• v.10 no.1
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• pp.69-74
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• 2000

The medium for erythritol production by Torula sp. in a 500-ml baffled flask was optimized to be 300 g/I sucrose, 10 g/I yeast extract, 3 g/I $KH_2PO_4$, and 10 mg/I $CuSO_4{\cdot}5H_2O{\;}at{\;}34^{\circ}C$ with initial pH of 5.5. Using this optimal medium, erythritol of 166 g/I was obtained after 140 h of cultivation, corresponding to 55.3% of the erythritol yield from sucrose with a productivity of 1.11 g/I/h. Optimal concentrations of carbbon and nitrogen sources in a fermentor were higher than that in a flask due to the higher oxygen supply of the fermentor. Employing the medium containing 300 g/I or 400 g/I sucrose for the determination of optimal C/N ratio, the C/N ratio was found to be more important than the nitrogen concentration for effective erythritol production, The optimal ratio of yeast extract to sucrose (g/g) was 20. The yield and productivity of erythritol were maximal in the medium containing 400 g/I sucrose and 20 g/I yeast extract. when dissolved oxygen in the culture was increased, the cell mass increased but the erythritol production was manimal in the range of 5 to 10% of dissolved oxygen. Under the optimal the rane of 5 to 10% of dissolved oxygen. Under the optimal culture condition of the fermentor, a final erythritol concentration of 200 gI was obtained after 120 h with a yield of 50% and the productivity was 1.67 g/I/h. The yield was the highest among erythritol-producting microorganisms

• The Journal of the Convergence on Culture Technology
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• v.8 no.4
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• pp.213-217
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• 2022

It is a time when a sustainable Korean Culture Festival strategy is needed. The Korean Culture Festival is a Hallyu Culture Festival that comprehensively introduces various Korean cultures such as Korean food, beauty, and fashion with the focus on K content, which has been leading the global craze since 2020. As the core project of the promotion of Neo Hallyu by the Ministry of Culture and Gymnasium, the 2021 Korean Culture Festival, which was based on the World K-Pop concert and the K-Culture Fan Fair, including holding the first face-to-face concert that applied the stage of real-world content after Covid-19, as well as conducting and exhibiting various fan participation challenges, must now make the leap to the global Hallyu Culture Festival. To this end, it can consist of drama, K-pop, K-Culture Fan Fair, K-Meetup, K-Culture Parade, and Awards. This distinction shows a classic festival program centered around the prosumer content that drives the Korean Wave, and in order for this philosophy to be effectively linked to its contemporaries, a Business to Business (B2B) and Business to Consumer (B2C) 'Techtainment Strategy' is needed to acquire potential customers through learned playfulness.

• Journal of Microbiology
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• v.44 no.6
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• pp.680-684
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• 2006

Twenty isolates resistant to seven quinolones were isolated from major rivers in Korea. All isolates had three mutations, Ser83$\rightarrow$Leu and Asp87$\rightarrow$Asn in GyrA and Ser80$\rightarrow$Ile or Ser80$\rightarrow$Arg in ParC and three isolates had an additional mutation Glu84$\rightarrow$Gly or Glu84$\rightarrow$Val in ParC. In addition, a clonal spread was not found in these isolates.

• Journal of Information Technology Applications and Management
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• v.18 no.4
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• pp.131-148
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• 2011

Our work empirically investigates the cultural differences of Social Networking Service (SNS) users in China, Korea and U.S. We construct a survey questionnaire from existing literature and test it for reliability, validity, and model fit. Then we collect data and validate the cultural differences of SNS users in three nations. Our results show different rankings from existing literature in cultural dimensions about three nations. In terms of masculinity, we find China > U.S. > Korea, similar to Hofstede. In individualism, we find U.S. > Korea > China, different from Hofstede (U.S. > China > Korea). In power distance, it is shown that Korea > China > U.S., different from Hofstede (China > Korea > U.S.). Uncertainty avoidance is found that U.S. > Korea > China, lowered ranking of Korea from the top among three nations in Hofstede. We find that these outcomes would be useful in updating national culture of the three nations and for future research about cultural impacts on SNS adoption.

• Journal of Microbiology and Biotechnology
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• v.1 no.1
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• pp.79-83
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• 1991

The effects of phosphate concentration in the medium, feeding of biosynthetic precursor, and the addition of exogenous berberine on cell growth and berberine production were studied in cell suspension cultures of Thalictrum rugosum. The depletion of phosphate in the medium enhanced the specific productivity up to twofold with significant release of berberine into the medium. Extracellular berberine was 19% of the total in the culture without phosphate while it was 2-5% of total berberine in the culture with even low amounts of phosphate. Precursor feeding was not effective in enhancing alkaloid formation. Initial presence of exogenous berberine did not have much effect on cell growth and alkaloid production. It was found that the cells have the capacity to take up large quantities of berberine. When $500{\;}mg{\cdot}l^{-1}$ of berberine was added exogenously at the beginning, 81% of total berberine was found in the cells.

• Proceedings of the KSME Conference
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• 2008.11a
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• pp.1411-1414
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• 2008

It is reported that mechanical stimulation takes a role in improving cell growth in skeletal system. And various research groups have showed that developed bioreactor to stimulate cell-seeded and threedimensional scaffold. In this study, we designed a custom-made bioreactor capable of applying controlled compression to cell-seeded agarose gel. This device consisted of a circulation system and compression system. In circular system, culture chamber was sealed for prohibiting contamination and media solution was circulated by pump. In compression system, mechanical stimuli were controlled by LabVIEW software and mechanical transfer system. Cell-encapsulated agarose gels were cultured for up to 7 days. There were significant differences between the number of cells grown in dynamic cell culture and in static cell culture from 3 days to 7 days.

• 한국생물공학회:학술대회논문집
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• 2003.10a
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• pp.536-539
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• 2003

A strong constitutive $P_{JH}$ promoter from Bacillus sp. was applied to overexpress the endoxylanase gene in Bacillus subtilis. The expression plasmid, pJHKJ4, was designed to contain the $P_{JH}$ promoter and open reading frame of endoxylanase including its own promoter. The plasmid was introduced into B. subtilis DB431 and the resulting transformant was grown on LB glucose medium. The endoxylanase activity in the culture supernatant reached about 140 unit/ml. The enzyme in the supernatant was efficiently concentrated to 70% by two-step treatments of ammonium sulfate saturation and ultrafiltration. The stabilization of concentrated enzyme solution at different storage temperatures was examined with various stabilizers such as NaCl, $CaCl_2$, sucrose, sorbitol, polyethylene glycol, and Tween-80.