• 대한생식의학회:학술대회논문집
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• 2004.06a
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• pp.70-71
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• 2004

• 대한생식의학회:학술대회논문집
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• 2005.06a
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• pp.116.2-117
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• 2005

• Proceedings of the Korea Society for Energy Engineering kosee Conference
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• 2010.04a
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• pp.194-194
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• 2010

• The Journal of Korean Obstetrics and Gynecology
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• v.19 no.4
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• pp.47-60
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• 2006

Purpose : This study was conducted to investigate the inhibitory effects of Scutellaria barbata D. D on on the cell proliferation of HeLa Cells. Methods : Human uterine cervical carcinoma HeLa cells were cultured in the 1%, 5% and 10% concentration of Scutellaria barbata D. D on solution for 24, 48 and 72 hours for the direct inhibitory effects of Scutellaria barbata D. D on. Then we examined the effect of Scutellaria barbata D. D on solution on the cell proliferation inhibition by XTT assay. DNA fragmentation, MAP kinase activity and caspase activity by FACS analysis in HeLa cells. Results : We found that the proliferation of HeLa cells was significantly decreased in Scutellaria barbata D. D on solution containing groups comparing with a control group in a concentration-dependant manner. When HeLa cells were cultivated for 24 hours with 5% Scutellaria barbata D. D on solution containing group, the percentage of HeLa cells with activated caspase was the highest. Scutellaria barbata D. D on solution reduced the MAP kinase activity of HeLa cells comparing with the control group. By the XTT assay, the cell's activity was decreased in 5% and 10% Scutellaria barbata D. D on solution containing groups in 24 and 72 hours cultivation and 10% group in 48 hours. DNA fragmentation and caspase-3 activity of HeLa cells, however, were changed insignificantly. Conclusion : From this study we could suggest that Scutellaria barbata D. D on is available to the inhibition and apoptosis of human cervical carcinoma cell line, HeLa cells in vitro.

• Proceedings of the Microbiological Society of Korea Conference
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• 2005.05a
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• pp.138-139
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• 2005

• Asian-Australasian Journal of Animal Sciences
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• v.27 no.4
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• pp.580-586
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• 2014

Intestinal epithelial cells (IECs) forming the barrier for the first-line of protection are interconnected by tight junction (TJ) proteins. TJ alteration results in impaired barrier function, which causes potentially excessive inflammation leading to intestinal disorders. It has been suggested that toll-like receptor (TLR) 2 ligands and some bacteria enhance epithelial barrier function in humans and mice. However, no such study has yet to be claimed in swine. The aim of the present study was to examine whether Bacillus subtilis could improve barrier integrity and protection against deoxynivalenol (DON)-induced barrier disruption in porcine intestinal epithelial cell line (IPEC-J2). We found that B. subtilis decreased permeability of TJ and improved the expression of zonula occludens (ZO)-1 and occludin during the process of forming TJ. In addition, ZO-1 expression of IPEC-J2 cells treated with B. subtilis was up-regulated against DON-induced damage. In conclusion, B. subtilis may have potential to enhance epithelial barrier function and to prevent the cells from DON-induced barrier dysfunction.

• Journal of the Korean Institute of Telematics and Electronics B
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• v.31B no.10
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• pp.140-148
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• 1994

In this paepr, the modified neocognitron, which has capability of recognizing multi-patterns in input image in one pass, is proposed. It is the hierarchical neural network composed of S and V layer which are able to extract features and of C layer with little effect from deformation, changes in size, shifts in position. S and V cells extract the features of all patterns in input image by applying DCC(don't care condition) to those cells. S and C cells also have position informations of extracted patterns. Position information is used in extracting good features without extracted features beting interfered one another. The proposed method is shorter in recognition time than the selective attention method with backward connection, because of recognizing multi-patterns in one passe. The modified neocognitron can recognizze attached multi-patterns because of using DCC and position informations.

• Parasites, Hosts and Diseases
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• v.38 no.4
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• pp.257-261
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• 2000

This study was focused on the effects of microfilament inhibitor, Cytochalasin D (CD) on the invasiveness of sporozoites of Cryptosporidiun spp. into the host cells. MDCK and AGS cell lines were used as host cells for C. parvum and C. muris, respectively. When MDCK cells were pretreated with CD for 1 hr before inoculation of the sporozoites, C. parvum infection was significantly inhibited when compared to the control cells. These inhibitory effects of CD on the rate of infection were dose-dependent. In addition, C. muris infection was hampered when AGS cell lines were pretreated with CD. However, the capability of invasiveness of the sporozoites into the host cells was not greatly influenced by the pretreatment of sporozoites with CD before infection. These results suggest that microfilaments of host cells, rather than parasites, play an important role for the invasion of Cryptosporidium spp.

• Proceedings of the KSAR Conference
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• 2004.06a
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• pp.272-272
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• 2004

The objective of this study is to test whether human embryonic stem cells expressing Nurr1 (Nurr1-transfected hES cells) could be expressed TH according to neuronal differentiation. As an effort to direct differentiation of hES (MB03 registered in NIH) cells to dopamine-producing neuronal cells, Nurr1 was transfected using conventional transfection protocol into MB03 cell and examined the expression of tyrosine hydroxylase (TH) after differentiation induced by retinoic acid (RA) and ascorbic acid (AA). (omitted)

• The Transactions of the Korean Institute of Electrical Engineers D
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• v.49 no.2
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• pp.81-87
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• 2000

This paper presents a new approach to the synthesis of two-dimensional arrays such as Atmel 6000 series FPGAs using internal don't cares. Basically complex terms which fits to the linear array of cells without further routing wires are generated and they are collected by OR/XOR operations. In previous methods, complex terms are collected only by XOR operations, which may not be effective for nearly unate functions. In this paper, we allow complex terms to be collected by OR operations in addition to XOR operations. First, complex terms that lies in the ON-set of the function are generated and collected by OR operations. The sub-function realized by the first stage becomes an internal don't cares and they are exploited in the second stage which generates complex terms collectable by XOR operation. Experimental results shows the efficacy of the proposed method compared to the previous methods.