Proceedings of the KSAR Conference (한국동물번식학회:학술대회논문집)
- 2004.06a
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- Pages.272-272
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- 2004
Genetically Modified Human Embryonic Stem Cells Expressing Nurr1 and Their Differentiation into Tyrosine Hydroxylase Positive Cells In Vitro
- Cho, Hwang-Yun (Maria Infertility Hospital Medical Institute/Maria Biotech) ;
- Lee, Chang-Hyun (Maria Infertility Hospital Medical Institute/Maria Biotec) ;
- Kim, Eun-Young (Maria Infertility Hospital Medical Institute/Maria Biotec) ;
- Lee, Won-Don (Maria Infertility Hospita) ;
-
Park, Sepill
(Maria Infertility Hospital Medical Institute/Maria Biotec)
;
- Lim, Jin-Ho (Maria Infertility Hospital)
- Published : 2004.06.01
Abstract
The objective of this study is to test whether human embryonic stem cells expressing Nurr1 (Nurr1-transfected hES cells) could be expressed TH according to neuronal differentiation. As an effort to direct differentiation of hES (MB03 registered in NIH) cells to dopamine-producing neuronal cells, Nurr1 was transfected using conventional transfection protocol into MB03 cell and examined the expression of tyrosine hydroxylase (TH) after differentiation induced by retinoic acid (RA) and ascorbic acid (AA). (omitted)