• Journal of Animal Science and Technology
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• 제45권6호
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• pp.901-910
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• 2003

본 연구는 한국재래돼지 염색체의 핵형 제시를 위하여 G-banding, C-banding 및 AgNORs를 분석하였다. 시험에 공시된 공시축은 축산기술연구소에서 선발 육종중인 재래돼지 종모돈 50두를 대상으로 각 개체별 혈액채취로서 혈액배양을 이용한 핵형 분석을 수행하였다. 한국재래돼지의 핵형은 38, XX 또는 XY로서 5쌍의 submetacentric chromosomes(Group I), 짧은 단완을 가진 2쌍의 acrocentric chromosomes(Group II), 5쌍의 metacentric chromosomes(Group III) 및 동원체가 말단부에 있는 6쌍의 acrocentric chromosomes(Group IV)로 구성된 36개의 상 염색체와 metacentric인 XX 또는 XY 성 염색체로 구성되어 있다. 재래돼지의 G-banding은 각 상동 염색체별 고유한 특징적 밴드 양상을 나타내고 있으며, 전체 염색체의 형태적 특징이나 대표적 landmarks는 국제표준핵형과 큰 차이가 없는 양상이다. 그러나 재래돼지의 경우 국제표준핵형에 비하여 보다 많은 band가 출현하였고 특히 1번, 3번, 5번, 6번, 7번, 8번, 13번, 14번, 15번, 16번, 17번, 18번 및 X 염색체에서 sub-bands의 분리를 나타내었다. 재래돼지의 C-banding은 비록 각 염색체들 간 heterochromatin의 양적 다형성이 존재하지만 거의 모든 상염색체의 동원체 부위에 C-bands가 나타나고, Y 염색체는 염색체의 전장에 걸친 heterochromatin의 분포를 보였다. AgNOR 염색에 의한 재래돼지의 NORs는 8번 및 10번 염색체의 동원체 부위에 확인되었고, 세포 당 NORs의 수는 2개에서 4개까지 관찰되었으며, 평균 2.13개로 분석되었다. 10번 염색체의 경우 모든 상동염색체에서 NORs가 나타나나 8번 염색체에서는 수적 다형성뿐만 아니라 양적 다형성을 나타내었다. 품종 간 NORs의 비교 분석에서 재래돼지의 NORs 수가 Yorkshire에 비해 유의적으로 높게 나타나 돼지의 NORs 분포 양상은 특히 8번 염색체에 있어 품종 간, 개체 간 및 세포 간에 다형적 변이 양상이 존재하는 것으로 사료된다.

• 대한수의학회지
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• 제60권4호
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• pp.241-244
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• 2020

Three neonatal pigs from the same litter in a domestic farm were born with skin lesions. Grossly, multiple well-circumscribed, round papules distributed over the skin of the three piglets. Two piglets were submitted for a diagnosis of skin disease. Microscopically, epidermal hyperplasia with ballooning degeneration of stratum spinosum keratinocytes was observed. Some keratinocytes contained eosinophilic intracytoplasmic inclusions and a central nuclear vacuole and chromatin margination. Swinepox (SWP) virus was detected by polymerase chain reaction and nucleotide sequencing, and Staphylococcus hyicus was isolated in skin lesions. Based on the gross findings and laboratory results, these piglets were diagnosed with congenital SWP with a secondary staphylococcal infection.

• 한국축산식품학회지
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• 제40권5호
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• pp.852-859
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• 2020

The muscle stem cells of domestic animals are of interest to researchers in the food and biotechnology industries for the production of cultured meat. For producing cultured meat, it is crucial for muscle stem cells to be efficiently isolated and stably maintained in vitro on a large scale. In the present study, we aimed to optimize the method for the enrichment of pig muscle stem cells using a magnetic-activated cell sorting (MACS) system. Pig muscle stem cells were collected from the biceps femoris muscles of 14 d-old pigs of three breeds [Landrace×Yorkshire×Duroc (LYD), Berkshire, and Korean native pigs] and cultured in skeletal muscle growth medium-2 (SkGM-2) supplemented with epidermal growth factor (EGF), dexamethasone, and a p38 inhibitor (SB203580). Approximately 30% of total cultured cells were nonmyogenic cells in the absence of purification in our system, as determined by immunostaining for cluster of differentiation 56 (CD56) and CD29, which are known markers of muscle stem cells. Interestingly, following MACS isolation using the CD29 antibody, the proportion of CD56⁺/CD29⁺ muscle stem cells was significantly increased (91.5±2.40%), and the proportion of CD56 single-positive nonmyogenic cells was dramatically decreased. Furthermore, we verified that this method worked well for purifying muscle stem cells in the three pig breeds. Accordingly, we found that CD29 is a valuable candidate among the various marker genes for the isolation of pig muscle stem cells and developed a simple sorting method based on a single antibody to this protein.

• 한국지반신소재학회논문집
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• 제23권2호
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• pp.63-75
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• 2024

마시는 물은 인간의 기본적인 생활권 보장을 위한 필수적 요소로 깨끗한 물에 대한 품질은 항상 확보되어야 한다. 하지만 국내 수도시설을 집중 설치하였던 2000년 초반을 기점으로 현재 노후 상수도 시설이 증가하고 있으며, 색도 및 탁도와 같은 물의 변색, 이물질 누출 등의 사고가 빈번히 발생하고 있다. 실제로 모든 관의 교체는 불가능하기 때문에 2021년 제정된 상수관망시설 유지관리업무 세부기준에서는 상수관 세척을 의무화 하였으며, 다양한 세척 공법들을 통해 유지관리가 이루어지고 있는 실정이다. 상수관 세척 공법 중 스왑피그 공법은 관 내 이물질을 물리적으로 제거하는 방법으로 세척 효율이 가장 높은 것으로 평가 받고 있다. 하지만, 세척과정에서 스왑피그의 파손 및 변형에 따라 세척 효율은 변화하며, 분실의 가능성이 존재한다. 따라서 본 연구에서는 피그 자체의 재료를 변경하여, 압축력 및 관 내벽과의 밀착력을 향상시키고자 하며, 세척 효율 극대화를 위해 회전 블레이드를 삽입된 회전식 스왑피그를 개발하였다. 또한, 분실 가능성을 제거하고, 피그의 위치 파악을 위해 고강도 와이어 및 권취 장치를 추가적으로 개발하여, 투입구부터 토출구까지를 연결하였다. 각 세부 기술의 성능 검증 이후에는 30m 구간의 테스트베드에서 본 기술을 적용하였으며, 현장 적용성 평가와 더불어 공정 시간을 측정하여 기술의 성능을 검증하였다.

• Journal of Microbiology and Biotechnology
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• 제18권10호
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• pp.1735-1740
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• 2008

Clone PERV-C (A3) env was isolated from the genomic DNA of domestic pig (Sus scrofa domesticus) in Korea to investigate the molecular properties of PERV-C. The nucleic acid homologies between the PERV-MSL (type C) reference and the PERV-C(A3) clone was 99% for env, but a single base pair deletion was found in the transmembrane (TM) region of the env open reading frame. To examine the functional characteristics of truncated PERV-C env, we constructed a replication-incompetent retroviral vector by replacing the env gene of the pCL-Eco retrovirus vector with PERV-C env. A retroviral vector bearing PERV-C/A chimeric envelopes was also created to complement the TM defect. Our results indicated that truncated PERV-C env was not infectious in human cells as expected. Interestingly, however, the vector with the PERV-C/A envelope was able to infect 293 cells. This observation suggests that recombination within PERV-C TM could render PERV-C infectious in humans. To further characterize PERV-C/A envelopes, we constructed an infectious molecular clone by using a PCR-based technique. This infectious molecular clone will be useful to examine more specific regions that are critical for human cell tropism.

• 한국동물생명공학회지
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• 제36권4호
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• pp.261-269
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• 2021

Because sows are industrially vital for swine production, monitoring for their health or disorder status is important to ensure high reproductive performance. Especially, ambient temperature changes in different season, especially during summer, are directly influenced to the reproductive performance of sows. Although the serum biochemical parameters are widely applied in the veterinary medicine with wide ranges for the physiological process, the values are also influenced by several factors such as age, breed, gender, and stress. In addition, domestic sows in Korea-specific reference interval (RI) for serum biochemistry has not been established yet. Therefore, the present study was aimed to evaluate seasonal variation of RIs in the serum biochemistry in domestic sows in Korea at different seasons and to establish normal RIs using a RI finding program (Reference Value Advisor). Significant difference (p < 0.05) on the different seasons were identified in several serum biochemical parameters including BUN, CRE, GGT, GLU, ALB, TP, LDH and Na in sows. Therefore, we further established RIs, specific in domestic sows in Korea regardless of season. The established RIs based on the serum biochemical values provide a baseline for interpreting biochemical results in the domestic sows in Korea, regardless of seasonal effect. It may contribute to develop a strategy for better reproductive performance by improving breeding management practice and evaluating health of pig herds, which facilitate to avert the economic loss in summer infertility in sows.

• 한국축산식품학회지
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• 제38권4호
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• pp.794-805
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• 2018

To identify correlation between fresh meat and processed meat products, we performed canonical correlation analysis (CCA) to predict the relationship between pork supply and meat product production in Korea. Results of CCA showed a canonical correlation of 0.8576 in the first canonical pair (p<0.01). The production of meat products showed the highest correlation with pork import but the lowest correlation with the production of domestic pork. Although Korean consumer preferred meat products produced by fresh domestic pork, inexpensively imported pork with high share in meat products was supplied in the market. Therefore, securing domestically produced raw meat is important for expanding consumption of domestic meat products. Results of this study suggest that meat processor and pig producer can achieve the $6^{th}$ industrialization by combining the production of raw pork materials, meat processing, and sales service.

• 농업과학연구
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• 제37권2호
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• pp.267-270
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• 2010

This study was carried out to manufacture black charcoal board from domestic wood waste by using serum protein adhesive which is natural, environment-friendly and human-friendly. For the preparation of the serum protein adhesive, pig blood from slaughterhouse was centrifuged and serum was separated from corpuscles and concentrated to 30% by dry weight basis. The particle size of charcoal from domestic wood waste for this study was #6-60. Hot pressing schedule was $170^{\circ}C$ and 40kgf/$cm^2$ (1 min)-10kgf/$cm^2$ (2.5 min)-40kgf/$cm^2$ (5 min). The black charcoal board made by the addition of 13% serum protein adhesive on dry weight basis gave 41.76kgf/$cm^2$ of bending strength, 8.12kgf/$cm^2$ of internal bonding strength, and excellent gas adsorption and workability.

• 한국동물위생학회지
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• 제45권1호
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• pp.13-18
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• 2022

African swine fever (ASF) is fatal to domestic pigs and wild boars (Sus scrofa) and affects the domestic pig industry. ASF is transmitted directly through the secretions of infected domestic pigs or wild boars, an essential source of infection in disease transmission. ASFV is also very stable in the environment. Thus, the virus is detected in the surrounding environment where ASF-infected carcasses are found. In this study, ASF infection monitoring was conducted on the swab and whole blood samples from wild animals, various hematopoietic arthropod samples that could access infected wild boar carcasses or habitats to cause maintenance and spread of disease, and soil samples of wild boar habitats. ASF viral DNA detection was confirmed negative in 317 wildlife and environmental samples through a real-time polymerase chain reaction. However, ASF occurs in the wild boars and spreads throughout the Korean peninsula. Therefore, it is necessary to trace the route of ASF virus infection by a continuous vector. Additional monitoring of various samples with potential ASF infection is needed to help the epidemiologic investigation and disease prevention.

• 대한수의학회지
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• 제45권1호
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• pp.63-70
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• 2005

African swine fever (ASF) is an infectious disease of domestic and wild pigs for which there is no vaccine in the world. A proper surveillance of viral activity and a timely response to ASF outbreaks depend upon the rapid diagnosis of ASF viral infection. Internationally prescribed enzyme-linked immunosorbent assay (ELISA) is a fast, sensitive test routinely used in the diagnosis of the ASF. However, inactivated whole ASF virus antigen used in this test is a tedious to prepare and has a risk of outside exposure of infectious virus by laboratory accident during the preparation. An ASF virus noninfectious recombinant antigen is a safe and easily produced alternative antigen for use in diagnostic assay. We have cloned the ORF O61R gene of the ASF virus to generate a recombinant baculovirus producing the p12 protein in insect cells under control of the polyhedrin promoter as non-fusion protein. When used in an indirect ELISA, the p12 antigen showed reactivity with all known ASF positive pig sera but not with negative pig sera. Our results indicated that the p12 protein would be one of alternative antigens for diagnosis of the ASF.