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Expression and diagnostic application of p12 protein of African swine fever virus by recombinant baculovirus  

Choi, Kang-Seuk (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Quarantine Service)
Choi, Cheong-up (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Quarantine Service)
Kim, Yong-Joo (National Veterinary Research and Quarantine Service, Ministry of Agriculture and Quarantine Service)
Publication Information
Korean Journal of Veterinary Research / v.45, no.1, 2005 , pp. 63-70 More about this Journal
Abstract
African swine fever (ASF) is an infectious disease of domestic and wild pigs for which there is no vaccine in the world. A proper surveillance of viral activity and a timely response to ASF outbreaks depend upon the rapid diagnosis of ASF viral infection. Internationally prescribed enzyme-linked immunosorbent assay (ELISA) is a fast, sensitive test routinely used in the diagnosis of the ASF. However, inactivated whole ASF virus antigen used in this test is a tedious to prepare and has a risk of outside exposure of infectious virus by laboratory accident during the preparation. An ASF virus noninfectious recombinant antigen is a safe and easily produced alternative antigen for use in diagnostic assay. We have cloned the ORF O61R gene of the ASF virus to generate a recombinant baculovirus producing the p12 protein in insect cells under control of the polyhedrin promoter as non-fusion protein. When used in an indirect ELISA, the p12 antigen showed reactivity with all known ASF positive pig sera but not with negative pig sera. Our results indicated that the p12 protein would be one of alternative antigens for diagnosis of the ASF.
Keywords
African swine fever; p12; recombinant baculovirus; ELISA; diagnosis;
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