Browse > Article

Isolation and Characterization of PERV-C env from Domestic Pig in Korea  

Park, Sung-Han (Department of Microbiology and Institute of Basic Science, Dankook University)
Bae, Eun-Hye (Department of Microbiology and Institute of Basic Science, Dankook University)
Park, Sang-Min (Department of Microbiology and Institute of Basic Science, Dankook University)
Park, Jin-Woo (Department of Microbiology and Institute of Basic Science, Dankook University)
Lim, Mi-Suk (Department of Microbiology and Institute of Basic Science, Dankook University)
Jung, Yong-Tae (Department of Microbiology and Institute of Basic Science, Dankook University)
Publication Information
Journal of Microbiology and Biotechnology / v.18, no.10, 2008 , pp. 1735-1740 More about this Journal
Abstract
Clone PERV-C (A3) env was isolated from the genomic DNA of domestic pig (Sus scrofa domesticus) in Korea to investigate the molecular properties of PERV-C. The nucleic acid homologies between the PERV-MSL (type C) reference and the PERV-C(A3) clone was 99% for env, but a single base pair deletion was found in the transmembrane (TM) region of the env open reading frame. To examine the functional characteristics of truncated PERV-C env, we constructed a replication-incompetent retroviral vector by replacing the env gene of the pCL-Eco retrovirus vector with PERV-C env. A retroviral vector bearing PERV-C/A chimeric envelopes was also created to complement the TM defect. Our results indicated that truncated PERV-C env was not infectious in human cells as expected. Interestingly, however, the vector with the PERV-C/A envelope was able to infect 293 cells. This observation suggests that recombination within PERV-C TM could render PERV-C infectious in humans. To further characterize PERV-C/A envelopes, we constructed an infectious molecular clone by using a PCR-based technique. This infectious molecular clone will be useful to examine more specific regions that are critical for human cell tropism.
Keywords
PERV-C; pseudotype assay; xenotransplantation; envelope;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
Times Cited By Web Of Science : 0  (Related Records In Web of Science)
연도 인용수 순위
1 Aguilar, H. C., W. F. Anderson, and P. M. Cannon. 2003. Cytoplasmic tail of Moloney murine leukemia virus envelope protein influences the conformation of the extracellular domain: Implication for mechanism of action of the R peptide. J. Virol. 77: 1281-1291   DOI   ScienceOn
2 Bartosch, B., R. A. Weiss, and Y. Takeuchi. 2002. PCR-based cloning and immunocytological titration of infectious porcine endogenous retrovirus subgroup A and B. J. Gen. Virol. 83: 2231-2240   DOI
3 Bobkova, M., J. Stitz, M. Engelstadter, K. Cichutek, and C. J. Buchholz. 2002. Identification of R-peptides in envelope proteins of C-type retroviruses. J. Gen. Virol. 83: 2241-2246   DOI
4 Fishman, J. A. 1998. Infection and xenotransplantation. Developing strategies to minimize risk. Ann. N. Y. Acad. Sci. 862: 52-66   DOI
5 Le Tissier, P., J. P. Stoye, Y. Takeuchi, C. Patience, and R. A. Weiss. 1997. Two sets of human-tropic pig retrovirus. Nature 389: 681-682   DOI   ScienceOn
6 Preuss, T., N. Fischer, K. Boller, and R. R. Tonjes. 2006. Isolation and characterization of an infectious replication-competent molecular clone of ecotropic porcine endogenous retrovirus class C. J. Virol. 80: 10258-10261   DOI   ScienceOn
7 Pyra, H., J. Böni, and J. Schüpbach. 1994. Ultrasensitive retrovirus detection by a reverse transcriptase assay based on product enhancement. Proc. Natl. Acad. Sci. USA 51: 1544-1548
8 Sahs, D. H., M. Sykes, S. C. Robson, and D. K. Cooper. 2001. Xenotransplantation. Adv. Immunol. 79: 129-223   DOI
9 Song, M. S., Y. H. Joo, E. H. Lee, J. Y. Shin, C. J. Kim, K. S. Shin, M. H. Sung, and Y. K. Choi. 2006. Genetic characterization of encephalomyocarditis virus isolated from aborted swine fetus in Korea. J. Microbiol. Biotechnol. 16: 1570-1576   과학기술학회마을
10 Takeuchi, Y., C. Patience, S. Magre, R. A. Weiss, P. T. Banerjee, P. Le Tissier, and J. P. Stoye. 1998. Host range and interference studies of three classes of pig endogenous retrovirus. J. Virol. 72: 9986-9991
11 Chiang, C. Y., Y. R. Pan, L. F. Chou, C. Y. Fang, S. R. Wang, C. Y. Yang, and H. Y. Chang. 2007. Functional epitopes on porcine endogenous retrovirus envelope protein interacting with neutralizing antibody combining sites. Virology 10: 361: 364-371   DOI   ScienceOn
12 Gemeniano, M., O. Mpanju, D. R. Salomon, M. V. Eiden, and C. A. Wilson. 2006. The infectivity and host range of the ecotropic porcine endogenous retrovirus, PERV-C, is modulated by residues in the C-terminal region of its surface envelope protein. Virology 346: 108-117   DOI   ScienceOn
13 Jung, Y., T. Wu, and C. A. Kozak. 2004. Novel host range and cytopathic variant of ecotropic friend murine leukemia virus. J. Virol. 78: 12189-12197   DOI   ScienceOn
14 Wilson, C. A., S. Wong, M. Vanbrocklin, and M. J. Federspiel. 2000. Extended analysis of the in vitro tropism of porcine endogenous retrovirus. J. Virol. 74: 49-56   DOI   ScienceOn
15 Akiyoshi, D. E., M. Denaro, H. Zhu, J. L. Greenstein, P. Banerjee, and J. A. Fishman. 1998. Identification of a fulllength cDNA for an endogenous retrovirus of miniature swine. J. Virol. 72: 4503-4507
16 Specke, V., S. J. Tacke, K. Boller, J. Schwendemann, and J. Denner. 2001. Porcine endogenous retroviruses: In vitro host range and attempts to establish small animal models. J. Gen. Virol. 82: 837-844   DOI
17 Ericsson, T. A., Y. Takeuchi, C. Templin, G. Quinn, S. F. Farhadian, J. C. Wood, B. A. Oldmixon, K. M. Suling, J. K. Ishii, Y. Kitagawa, T. Miyazawa, D. R. Salomon, R. A. Weiss, and C. Patience. 2003. Identification of receptors for pig endogenous retrovirus. Proc. Natl. Acad. Sci. USA 100: 6759-6764
18 Wilson, C. A., S. Wong, J. Muller, C. E. Davidson, T. M. Rose, and P. Burd. 1998. Type C retrovirus released from porcine primary peripheral blood mononuclear cells infects human cells. J. Virol. 72: 3082-3087
19 Bach, F. H., J. A. Fishman, N. Daniels, J. Proimos, B. Anderson, C. B. Carpenter, L. Forrow, S. C. Robson, and H. V. Fineberg. 1998. Uncertainty in xenotransplantation: Individual benefit versus collective risk. Nat. Med. 4: 141-144   DOI   ScienceOn
20 Chapman, L. E., T. M. Folks, D. R. Salomon, A. P. Pattersom, T. E. Eggerman, and P. D. Noguchi. 1995. Xenotransplantation and xenogeneic infections. N. Engl. J. Med. 333: 1498-1501   DOI   ScienceOn
21 Kim, E., H. R. Poo, M. H. Sung, and C. J. Kim. 2005. Mature HIV-like particles produced from single semliki forest virus-derived expression vector. J. Microbiol. Biotechnol. 15: 1229-1239   과학기술학회마을
22 Patience, C., Y. Takeuchi, and R. A. Weiss. 1997. Infection of human cells by an endogenous retrovirus of pigs. Nat. Med. 3: 282-286   DOI   ScienceOn
23 Harrison, I., Y. Takeuchi, B. Bartosch, and J. P. Stoye. 2004. Determinants of high titer in recombinant porcine endogenous retroviruses. J. Virol. 78: 13871-13879   DOI   ScienceOn
24 Todaro, G. J., R. E. Benveniste, M. M. Lieber, and C. J. Sherr. 1974. Characterization of a type C virus released from the porcine cell line PK(15). Virology 58: 65-74   DOI   ScienceOn