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Description of Vishniacozyma terrae sp. nov. and Dioszegia terrae sp. nov., Two Novel Basidiomycetous Yeast Species Isolated from Soil in Korea

  • Soohyun Maeng;Yuna Park;Gi-Ho Sung;Hyang Burm Lee;Myung Kyum Kim;Sathiyaraj Srinivasan
    • Mycobiology
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    • v.50 no.6
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    • pp.439-447
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    • 2022
  • Two strains, YP344 and YP579 were isolated from soil samples in Pocheon City, Gyeonggi Province, South Korea. The strains YP344 and YP579 belong to the genus Vishniacozyma and Dioszegia, respectively. The molecular phylogenetic analysis showed that the strain YP344 was closely related to Vishniacozyma peneaus. Strain YP344T differed by four nucleotide substitutions with no gap (0.70%) in the D1/D2 domain of the LSU rRNA gene and 16 nucleotide substitutions with 8 gaps (5.76%) in the ITS region. On the other hand, the strain YP579T varied from the type strain of the most closely related species, Dioszegia zsoltii var. zsoltii, by 6 nucleotide substitutions with four gaps (1.64%) in the D1/D2 domain of LSU rRNA gene and 26 nucleotide substitutions with 14 gaps (8.16%) in the ITS region. Therefore, the name Vishniacozyma terrae sp. nov. and Dioszegia terrae sp. nov. are proposed, with type strains YP344T (KCTC27988T) and YP579T (KCTC 27998T), respectively.

Neodothiora pruni sp. nov., a Biosurfactant-Producing Ascomycetous Yeast Species Isolated from Flower of Prunus mume

  • Jeong-Seon Kim;Miran Lee;Jun Heo;Soon-Wo Kwon;Bong-Sik Yun;Yiseul Kim
    • Mycobiology
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    • v.51 no.6
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    • pp.388-392
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    • 2023
  • A yeast strain, designated as JAF-11T, was isolated from flower of Prunus mume Sieb. et Zucc. in Gwangyang, Republic of Korea. Phylogenetic analysis showed that strain JAF-11T was closely related to Neodothiora populina CPC 39399T with 2.07 % sequence divergence (12 nucleotide substitutions and three gaps in 581 nucleotides) in the D1/D2 domain of the large subunit (LSU) rRNA gene, and Rhizosphaera macrospora CBS 208.79T with 4.66 % sequence divergence (25 nucleotide substitutions and five gaps in 535 nucleotides) in the internal transcribed spacer (ITS) region. Further analysis based on the concatenated sequences of the D1/D2 domain of the LSU rRNA gene and the ITS region confirmed that strain JAF-11T was well-separated from Neodothiora populina CPC 39399T. In addition to the phylogenetic differences, strain JAF-11T was distinguished from its closest species, Neodothiora populina CPC 39399T and Rhizosphaera macrospora CBS 208.79T belonging to the family Dothioraceae by its phenotypic characteristics, such as assimilation of carbon sources. Hence, the name Neodothiora pruni sp. nov. is proposed with type strain JAF-11T (KACC 48808T; MB 850034).

A Proposal for the Online ADR Model Building on Electronic Commerce Dispute Resolution (전자상거래 분쟁해결을 위한 온라인 ADR 모델구축에 관한 연구)

  • Kim, Sun-Kwang
    • International Commerce and Information Review
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    • v.8 no.2
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    • pp.101-117
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    • 2006
  • "Online Alternative Dispute Resolution" can refer to the use of online methods of dispute resolution to resolve disputes arising either online or offline. The range of disputes covered by online ADR has been broad : from family law to internet domain name disputes : from small transaction to insurance disputes. Online and offline consumer disputes have been a major focus of online ADR sites. This article propsed that the mediator should explain the process and the mediator's role so as to forestall misunderstanding on that score. And mediators should consider including in either usual mediation agreements additional provisions applicable to communications by email. Online ADR sites should be designed 1) to provide a simple, easily understandable process, 2) to provide detailed information on process, cost and speed, 3) to enable users to move between online and offline processes, 4) to have authentication processes for parties and documents, 5) to have automatic translation system for language barriers. And Government should play an important role in assisting people to adapt technically and emotionally to new technology through information, training and ongoing support. The days of live online television-quality videoconferencing have not yet arrived. Until then, we must hone our skills with the written word.

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Genotyping of HLA-A by Polymerase Chain Reaction-Sequence Specific Primer (Polymerase Chain Reaction-Sequence Specific Primer를 이용한 HLA-A 유전자의 DNA 다형성 조사)

  • Jang, Soon-Mo
    • Korean Journal of Clinical Laboratory Science
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    • v.40 no.2
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    • pp.94-97
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    • 2008
  • The human leukocyte antigen (HLA) is the name of the major histocompatibility complex (MCH) in humans. The superlocus contains a large number of genes related to immune system function in humans. This group of genes resides on chromosome 6. and encode cell surface antigen-presenting proteins and many other genes. HLA class I antigen (A, B & C) present peptides from inside the cell. These peptides are produced from digested proteins that are broken down in the lysozymes. Most expressed HLA loci exhibit a remarkable degree of allelic polymorphism, which derives from sequence differences predominantly localized to discrete hypervariable regions of the amino terminal domain of the molecule. In this sutdy, the HLA-A genotypes were determined in twenty students unrelated koreans using the PCR-SSP (Polymerase Chain Reaction-Sequence Specific Primer) technique. Several specific primer pairs in assigning the HLA-A gene were used (A*0201, A*33, A*2401). The results of PCR-SSP, the HLA-A*0201 primer was detected eleven (55%), the HLA-A*33 were detected seven (35%) and the HLA-A*2401 were detected seven (35%). This study shows that the PCR-SSP technique is relatively simple, fast and a practical tool for the determination of the HLA-A genotypes.

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A Service Discovery Scheme Based on NAPTR Resource Record (NAPTR 자원레코드 기반의 서비스 탐색기법)

  • 권성호;김희철
    • Journal of Korea Society of Industrial Information Systems
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    • v.8 no.3
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    • pp.69-75
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    • 2003
  • NAPRT(Naming Authority Pointer) is a type of resource record specified in IETF RFC 2915. NAPTR enables to register various services in the Domain Name Systems and thus provides a way to discover services available on specific hosts. This paper describes the design and implementation of a Proxy DNS system aimed at supporting NAPTRs. The goal of this work is to study on the feasibility of the service discovery registered in DNS via NAPTR records and provides the result for simplicity and extensibility of implementation through the implementation of a actual Test-bed system This research result can be applied to service discovery in the resource information management for high performance GRE environments as well as to the implementation of DNS infrastructure for the ENUM.

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Development of Web Game and Contents Support System Using the Integrated Point Engine (포인트 통합 엔진을 이용한 웹 게임 및 컨텐츠 제공 시스템의 개발)

  • Kang, Sung-Kwan;Ahn, Tae-Hong
    • Journal of Korea Game Society
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    • v.4 no.4
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    • pp.25-30
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    • 2004
  • Recently, it is increasing a users requiring actually and a variety of service due to a growth of wet based technique and network technique. In services, the interest in web and mobile contents domain is increasing rapidly. The most contents supported point or a E-Money as result of contents's usage. But, the point system be in name only due to a lack of a contents using a point or a related service. As a solution for this problem, this paper designed a supported system that integrated a point system supporting others contents and can associated to a game using that point. we made a simple game and supporting contents in web for simulating the designed system. Also, we realized the mobile system for communicating a data causing in web.

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A Scheme to Improve QoS in a Multi-Virtual-Hosting Server (다중 Virtual Hosting Server의 QoS 향상 기법에 관한 연구)

  • Ryou, Sang-Woo;Ko, Soung-Jun;Lee, Sang-Moon;Kim, Hag-Bae;Park, Jin-Bae;Jang, Whie
    • Journal of Institute of Control, Robotics and Systems
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    • v.8 no.4
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    • pp.303-307
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    • 2002
  • Virtual hosting is a typical service to connect each directory of site and domain name. If traffic amounts may increase at one site present in the server, then it affects traffic amounts of other sites as well (including the sites which have flew requests). To overcome this problem, we suggest a simple feedback-control concept for the system by periodically monitoring the traffic and properly actuating traffic dispersions by investigating the log file. Specifically, large files are to be served in a backup server (to reduce the workload of the main server) by changing their own URL's in html format. In other words, it automatically redistributes the workload by using the URL. Furthermore, we also use the redirecting method by just adding html tags to html header. This method efficiently handles the workload and maintains the capability of the server effectively to the varying workload.

A study on content structure of literature search methodology (문헌조사법의 내용구조에 관한 고찰)

  • 김정소;박준식
    • Journal of Korean Library and Information Science Society
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    • v.10
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    • pp.27-58
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    • 1983
  • The purpose of this study is to clarify the educational literature search methodology objectives and the content structure of a course in bibliography. The content of this study can be summarized as follows: 1. The concept and domain of literature search methodology, which has traditionally gone by the name of a guide to use of literature and library, are described. 2. The need of incorporating the methodology into regular college curriculum was investigated in view of the ideology of college education. 3. The background, process, and current status of offering a course in the methodology as a part of regular college curriculum were examined. 4. The educational objective of a course in the methodology was set at the successful activities in self study, life-long-education, and special topic research. 5. Considering the fact that technical knowledge and propositional knowledge should be realized simultaneously, that educational experience should go hand in hand with materials and library, and that knowledge should be constructed systematically and logically, the content structure of the methodology were divided into the following six categories: a) the significance of the methodology of literature search b) the college library and the activities of literature search c) types and characteristics of materials d) literature search by catalog e) explanation of basic bibliography f) research and literature search

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The QoS Guarantees of Multimedia Traffic using MIP-DUDN with RSVP in Mobile Computing Environments (이동 컴퓨팅 환경에서 RSVP가 적용된 MIP-DUDN을 이용한 멀티미디어 트래픽의 QoS 보장)

  • Han, Seung-Jin;Lee, Jeong-Hyeon
    • The KIPS Transactions:PartC
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    • v.8C no.5
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    • pp.667-676
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    • 2001
  • We have solved a problem occurring in case that RSVP is applied to existing wired network in order to guarantee a QoS of multimedia traffic in mobile computing environment in this paper. If MN moves to another area when Mobile IP is applied to RSVP, HA makes new tunnel to FA because SESSION ID of MN is changed. However, we suggest MIP-DUDN method, which applied RSVP. Although MN moves to another area in this method, it does not make new tunnel because it eliminates the problem of existing Triangle Routing Problem. We design the new protocol of RMIP-DUDN and compare with the method of existing Mobile IP applied to RSVP.

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NAT-PT Operation for IPv4 and IPv6 Nodes to Coexist (IPv4와 IPv6의 연동과 호환을 위한 NAT-PT에 관한 연구)

  • 이승민;민상원;김용진;박수홍
    • Proceedings of the Korean Information Science Society Conference
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    • 2000.10c
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    • pp.532-534
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    • 2000
  • IPv4(Internet Protocol version 4)의 차세대 버전인 IPv6는 기존 IPv4의 문제점인 주소부족 및 새로운 부가기능 등의 필요성 때문에 IETF(Internet Engineering Task Force)에서 IPv4를 대체하기 위해 개발한 프로토콜이다. 하지만 IPv4를 어느 한순간에 IPv6로 대체하는 것은 불가능하기 때문에 기존 IPv4와의 호환 및 연동을 위한 터널링 및 기타 여러 가지 transition mechanism들이 고려되고 있다. 이러한 mechanism들 중에서 최근 표준으로 발표된 NAT-PT(Network Address Translation $\square$ Protocol Translation)는 DNS-ALG (Domain Name System $\square$ Application Level Gateway)라는 개념을 도입하여 IPv4와 IPv6간의 양방향 주소변환 및 프로토콜 변환기능을 수행한다. 각각의 기능에서 주소 변환은 주소크기와 형식이 다른 IPv4와 Pv6간의 서로 상대 노드를 엑세스할 수 있도록 하고 프로토콜 변환은 다른 버전의 프로토콜을 사용하는 노드들이 상대노드가 보낸 패킷의 헤더정보를 이해할 수 있도록 한다. 그리고 이러한 기본적인 변환 외에 헤더정보의 정확한 전달을 위해서는 기존의 path MTU(Maximum Transgfer Unit) discovery 그리고 pseudo header checksum 등도 고려되어야 한다.

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