• BMB Reports
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• v.45 no.6
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• pp.360-364
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• 2012

Uptake of circulating glucose into the cells happens via the insulin-mediated signalling pathway, which translocates the glucose transporter 4 (GLUT4) vesicles from the intracellular compartment to the plasma membrane. Rab GTPases are involved in this vesicle trafficking, where Rab GTPases-activating proteins (RabGAP) enhance the GTP to GDP hydrolysis. TBC1D4 (AS160) and TBC1D1 are functional RabGAPs in the adipocytes and the skeletonal myocytes, respectively. These proteins contain two phosphotyrosine-binding domains (PTBs) at the amino-terminus of the catalytic RabGAP domain. The second PTB has been shown to interact with the cytoplasmic region of the insulin-regulated aminopeptidase (IRAP) of the GLUT4 vesicle. In this study, we quantitatively measured the ${\sim}{\mu}M$ affinity ($K_D$) between TBC1D4 PTB and IRAP using isothermal titration calorimetry, and further showed that IRAP residues 1-49 are the major region mediating this interaction. We also demonstrated that the IRAP residues 1-15 are necessary but not sufficient for the PTB interaction.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 1999.04a
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• pp.267-274
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• 1999

Since the seismic response of dams can be strongly influenced by the dam-reservior interaction in needs to be taken into account in the seismic design of dams. In general a substructure method is employed to solve the dam-reservoir interaction problem in which the dam body is modeled with finite elements and the infinite region of a reservoir using a transmitting boundary. When the water is modeled as a compressible fluid the equation is formulated in frequency domain. But nonlinear behavior of dam body cannot be studied easily in the frequency domain method. In this study time domain formulation of the dam-reservoir-soil interaction is proposed based onthe lumped parameter modeling of the reservoir region, The frequency dependent dynamic-stiffness coefficients of the reservoir are converted into frequency independent lumped-parameters such as masses dampers and springs. The soil-structure interactionis modeled using lumped parameters in similar way. the ground is assumed as a visco-elastic stratum on the rigid bedrock. The dynamic stiffnesses of the rigid surface foundation are calculated using the hyperelement method and are converted into lumped parameters. The application example demonstrated that the lumped parameter model gives almost identical results with the frequency domain formulation.

• Coupled systems mechanics
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• v.8 no.5
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• pp.393-414
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• 2019

This paper presents seismic analysis of concrete gravity dams considering soil-structure-fluid interaction. Displacement based plane strain finite element formulation is considered for the dam and foundation domain whereas pressure based finite element formulation is considered for the reservoir domain. A direct coupling method has been adopted to obtain the interaction effects among the dam, foundation and reservoir domain to obtain the dynamic responses of the dam. An efficient absorbing boundary condition has been implemented at the truncation surfaces of the foundation and reservoir domains. A parametric study has been carried out considering each domain separately and collectively based on natural frequencies, crest displacement and stress at the neck level of the dam body. The combined frequency of the entire coupled system is very less than that of the each individual sub-system. The crest displacement and neck level stresses of the dam shows prominent enhancement when coupling effect is taken into consideration. These outcomes suggest that a complete coupled analysis is necessary to obtain the actual responses of the concrete gravity dam. The developed methodology can easily be implemented in finite element code for analyzing the coupled problem to obtain the desired responses of the individual subdomains.

• Journal of the Computational Structural Engineering Institute of Korea
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• v.34 no.6
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• pp.427-435
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• 2021

An analysis considering the fluid-structure interaction is required to strictly evaluate the seismic behavior of facilities such as, environmental facilities and dams, that store fluids. Specifically, in the case of an infinite domain in the upstream direction, such as a dam-reservoir system, this should be carefully considered. In this study, we proposed a practical numerical model for both wave propagation and fluid-structure interaction analyses of an infinite domain, for a system with a semi-infinite domain such as a dam-reservoir system. This method was applicable to the time domain, and enabled accurate boundary analysis. For an infinite fluid domain, a small number of mid-point integrated acoustic finite elements were applied instead of a general acoustic finite element, and a viscous boundary was imposed on the outermost boundary. The validity and accuracy of the proposed method were secured by comparing analytic solutions of a reservoir having infinite domain, with the parametric analysis results, for the number of elements and the size of the modeling region. Furthermore, the proposed method was compared with other fluid-structure interaction methods using additional mass.

• Child Health Nursing Research
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• v.10 no.2
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• pp.165-172
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• 2004

The main purpose of this study is to examine the concepts appeared on research and provide future research directions in field of child health nursing. 205 studies of the total 318 studies had been analyzed first for the concepts of the client domain and this time 113 studies were analyzed for the practice, the client-nurse and the environmental domain in nursing. The practice domain includes mentalistitic, enactment, knowledge utilization, role related phenomenon, the client-nurse domain includes touch, communication, interaction phenomenon, and the environmental domain includes physical, social, symbolic environment. All were originally published between 1990 and 2000 in Korea. An analysis of concepts for this study was used the metaparadigm framework for nursing proposed by H. S. Kim(2000). 1. 103 studies belonged to the practice domain. Among them, 56(54％) studies used concepts related to enactment phenomenon like education(21.4％), giving information(7.1％), breast feeding(5.4％), caring(5.4％), airway suction(5.4％), nonnutritive sucking(5.4％). 44(43％) studies used concepts related to knowledge utilization like program development and evaluation of smoking, mother-infant interaction, home health nursing, obesity management. And only 3(3％) studies used role related concepts like quality of nursing, direction of health education, contents of child health nursing education. 2. Only 2(0.006％) studies belonged to the client-nurse domain. One concept is empathy in communication phenomenon and the other concept is role conflict in interaction phenomenon. 3. 8(0.02％) studies belonged to the environmental domain. Among them, 3 studies related to physical environment like space, noise and 5 studies related to social environment like social support, home environment. But the concept of symbolic environment was not used. The findings of this study provide the evidence that research related to the client-nurse domain and the environmental domain should be conducted actively to improve the practice of child health nursing. So that the research in field of child health nursing should be dealt with the concepts of four domains to develop knowledge systematically.

• Molecules and Cells
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• v.40 no.5
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• pp.355-362
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• 2017

The ${\beta}2$ integrins are cell surface transmembrane proteins regulating leukocyte functions, such as adhesion and migration. Two members of ${\beta}2$ integrin, ${\alpha}M{\beta}2$ and ${\alpha}X{\beta}2$, share the leukocyte distribution profile and integrin ${\alpha}X{\beta}2$ is involved in antigen presentation in dendritic cells and transendothelial migration of monocytes and macrophages to atherosclerotic lesions. ${\underline{R}}eceptor$ for ${\underline{a}}dvanced$ ${\underline{g}}lycation$ ${\underline{e}}nd$ ${\underline{p}}roducts$ (RAGE), a member of cell adhesion molecules, plays an important role in chronic inflammation and atherosclerosis. Although RAGE and ${\alpha}X{\beta}2$ play an important role in inflammatory response and the pathogenesis of atherosclerosis, the nature of their interaction and structure involved in the binding remain poorly defined. In this study, using I-domain as a ligand binding motif of ${\alpha}X{\beta}2$, we characterize the binding nature and the interacting moieties of ${\alpha}X$ I-domain and RAGE. Their binding requires divalent cations ($Mg^{2+}$ and $Mn^{2+}$) and shows an affinity on the sub-micro molar level: the dissociation constant of ${\alpha}X$ I-domains binding to RAGE being $0.49{\mu}M$. Furthermore, the ${\alpha}X$ I-domains recognize the V-domain, but not the C1 and C2-domains of RAGE. The acidic amino acid substitutions on the ligand binding site of ${\alpha}X$ I-domain significantly reduce the I-domain binding activity to soluble RAGE and the alanine substitutions of basic amino acids on the flat surface of the V-domain prevent the V-domain binding to ${\alpha}X$ I-domain. In conclusion, the main mechanism of ${\alpha}X$ I-domain binding to RAGE is a charge interaction, in which the acidic moieties of ${\alpha}X$ I-domains, including E244, and D249, recognize the basic residues on the RAGE V-domain encompassing K39, K43, K44, R104, and K107.

• Proceedings of the Earthquake Engineering Society of Korea Conference
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• 2000.04a
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• pp.443-450
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• 2000

In this study a numerical method for soil-pile interaction analysis buried in multi-layered half planes is presented in frequency domain using FE-BE coupling. The total soil-pile interaction system is divided into two parts so called far field and near field beam elements are used for modeling a pile and coupled with plain strain elements for soil modeling. Boundary element formulation using the multi-layered dynamic fundamental solution is adopted to the far field and coupled with near field modeled by finite elements. In order to verify the proposed soil-pile interaction analysis method the dynamic responses of a pile on multi-layered dynamic fundamental solution is adopted to the far field and coupled with near field modeled by finite elements. In order to verify the proposed soil-pile interaction analysis method the dynamic responses of a pile on multi-layered half-planes are performed and compared with experiment results. Through this developed method the dynamic response analysis of a pile buried in multi-layered half planes can be calculated effectively in frequency domain.

• Journal of Magnetics
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• v.18 no.4
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• pp.417-421
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• 2013

The dominant magnetization reversal behavior of electrodeposited CoPt samples with various thicknesses deposited at different current densities was the domain wall motion by means of wall pinning. The magnetic interaction mechanism was dipolar interaction for all samples. The dipolar interaction strength was significantly affected by the sample thickness rather than by the current density, while the magnetic properties were closely related to the current density.

• Earthquakes and Structures
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• v.3 no.3_4
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• pp.563-580
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• 2012

We modify the formulation of a recently developed absorbing boundary condition (ABC), the perfectly matched discrete layers (PMDL), to incorporate the excitation coming from the exterior such as earthquake waves. The modified formulation indicates that the effect of the exterior excitation can be incorporated into PMDL ABCs (traditionally designed to treat only interior excitation) simply by applying appropriate forces on the nodes connected to the first PMDL layer. Numerical results are presented to clearly illustrate the effectiveness of the proposed method.

• BMB Reports
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• v.44 no.9
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• pp.572-577
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• 2011

Elevated phospholipase D (PLD) expression prevents cell cycle arrest and apoptosis. However, the roles of PLD isoforms in cell proliferation and apoptosis are incompletely understood. Here, we investigated the physiological significance of the interaction between PLD2 and protein kinase CKII (CKII) in HCT116 human colorectal carcinoma cells. PLD2 interacted with the CKII${\beta}$ subunit in HCT116 cells. The C-terminal domain (residues 578-933) of PLD2 and the N-terminal domain of CKII${\beta}$ were necessary for interaction between the two proteins. PLD2 relocalized CKII${\beta}$ to the plasma membrane area. Overexpression of PLD2 reduced CKII${\beta}$ protein level, whereas knockdown of PLD2 led to an increase in CKII${\beta}$ expression. PLD2-induced CKII${\beta}$ reduction was mediated by ubiquitin-dependent degradation. The C-terminal domain of PLD2 was sufficient for CKII${\beta}$ degradation as the catalytic activity of PLD2 was not required. Taken together, the results indicate that the C-terminal domain of PLD2 can regulate CKII by accelerating CKII${\beta}$ degradation in HCT116 cells.