• Proceedings of the Botanical Society of Korea Conference
• /
• 2002.04a
• /
• pp.62-72
• /
• 2002

This study has investigated the biosynthesis and function of the heavy metal binding peptides, the phytochelatins, in plants. PCs are synthesised enzymatically from glutathione by the enzyme PC synthase in the presence of heavy metal ions. Using Arabidopsis thaliana as a model organism cadmium-sensitive, phytochelatin-deficient mutants have been isolated and characterised in previous studies. The cadl mutants have wildtype levels of glutathione, are PC deficient and lack PC synthase activity. Thus, the CADl gene has been proposed to encode PC synthase. The CADl gene was isolated by a positional cloning strategy The gene was mapped and a candidate identified. Each of four cadl mutants had a single base pair change in the candidate gene and the cadmium-sensitive, cadl phenotype was complemented by the candidate gene. This demonstrated the CADl gene had been cloned. A homologous gene in the fission yeast, Schizosaccharomyces pombe was identified through database searches. A targeted-deletion mutation of this gene was constructed and the mutant, like cadl mutants of Arabidopsis, was cadmium-sensitive and PC-deficient. A comparison of the redicted amino acid sequences reveals a highly conserved N-terminal region Presumed to be the catalytic domain and a variable C-terminal region containing multiple Cys residues proposed to be involved in activation of the enzyme by metal ions. Similar genes were also identified in animal species. The Arabidopsis CADl/AtPCSl and S. pombe SpbPCS genes were expressed in E. coli and were shown to be sufficient for glutathione-dependent, heavy metal activate PC synthesis in vitro, thus demonstrating these genes encode PC synthase enzymes. Using RT-PCR, AtPCSl expression appeared to be independent of Cd exposure. However, at higher levels of Cd exposure a AtPCSl-CUS reporter gene construct appeared to be more highly expressed. Using the reporter gene construct, AtPCSl was expressed most tissues. Expression appeared to be greater in younger tissues and same higher levels of expression was observed in some regions, including carpels and the base of siliques. AtPCS2 was a functional gene encoding an active PC synthase. However, its Pattern of expression and the phenotype of a mutant (or antisense line) have not been determined. Assuming the gene is functional then it has clearly been maintained through evolution and must provide some selective advantage. This implies that, at least in some cells or tissue, it is likely to be the dominant PC synthase expressed. This remains to be determined

• Geophysics and Geophysical Exploration
• /
• v.4 no.2
• /
• pp.25-33
• /
• 2001

Loop-loop electromagnetic (EM) survey in frequency domain has been carried out in order to provide basic solution to geotechnical applications. Source and receiver configuration may be horizontal co-planar (HCP) and/or vertical co-planar (VCP). Three quadrature components of mutual impedance ratio for each configuration are used to construct the subsurface image. For the purpose of obtaining the model response and validating the reasonable performance of the inversion, we obtained each responses of two-layered and three-layered earth models and two-dimensional (2-D) isolated anomalous body. The response of 2-D isolated anomalous body has been calculated using extended Born approximation for the solution of 2.5-D integral equation describing EM scattering problem. As a result of the least-squares inversion with variable Lagrangian multiplier, we could construct more resolvable image from HCP data than VCP data. Furthermore, joint inversion of HCP and VCP data made better stability and resolution of the inversion. Resistivity values, however, did not exactly match the true ones. Loop-loop EM field data was obtained with EM34-3XL system manufactured by Geonics Ltd. (Canada). Electrical resistivity survey was conducted on the same line for the comparison in advance. Since the constructed image from loop-loop EM data by 2-D inversion algorithm showed almost similar resistivity distribution to that from electrical resistivity one, we expect the developed 2.5-D loop-loop EM inversion program can be applied for the reconnaissance site survey.

• Journal of Family Resource Management and Policy Review
• /
• v.16 no.4
• /
• pp.171-194
• /
• 2012

This study focuses on immigrant women who are married to Korean men and who live in a multicultural family situation in Gyeonggi-do. These women experience acculturative stress and marital conflict and this study aims to determine how social support seeks to mediate the effects of those stressors. The women in this study participated in activities and received services from one of Gyeonggi-do Province's civic organizations, religious organizations, or social service organizations, such as the Social Welfare Agency and the Multicultural Family Support Center. In order to verify the study's hypothesis, the researchers used the following statistical analytical methods : t-test, two-way ANOVA and multi-regression analysis. Analysis of the study's results showed that the highest degree of marital conflict was found in the sub-zones and variables that were personal. Those variable were : the difference in mindset and values (personal domain), economic problems (communal living area), a child's upbringing and education issues (third party area), and the participant's sex life (in the marital relationship). The hypothesis was tested using the research model validation and the results are summarized as follows. First, in order to analyze the relationship between the marriage migration females' acculturative stress and the impact of that stress on marriage conflict, the hierarchical regression analysis was used. It identified that a direct correlation existed between acculturative stress and marriage conflict; where a higher degree of acculturative stress was present, a higher degree of marriage conflict was found. Second, the study found a statistical significance in the correlation and relationship (${\beta}$=.208, p<0.05) between the acculturative stress of the marriage migration female and material support. In other words, the material support can be seen as having a moderating effect on the acculturative stress, which is the independent variable, and the marital conflict, which is the dependent variable.

• Journal of Radiation Protection and Research
• /
• v.40 no.1
• /
• pp.55-64
• /
• 2015

Dose calculations which are a crucial requirement for radiotherapy treatment planning systems require accuracy and rapid calculations. The conventional radiotherapy treatment planning dose algorithms are rapid but lack precision. Monte Carlo methods are time consuming but the most accurate. The new combined system that Monte Carlo methods calculate part of interesting domain and the rest is calculated by neural can calculate the dose distribution rapidly and accurately. The preliminary study showed that neural networks can map functions which contain discontinuous points and inflection points which the dose distributions in inhomogeneous media also have. Performance results between scaled conjugated gradient algorithm and Levenberg-Marquardt algorithm which are used for training the neural network with a different number of neurons were compared. Finally, the dose distributions of homogeneous phantom calculated by a commercialized treatment planning system were used as training data of the neural network. In the case of homogeneous phantom;the mean squared error of percent depth dose was 0.00214. Further works are programmed to develop the neural network model for 3-dimensinal dose calculations in homogeneous phantoms and inhomogeneous phantoms.

• Journal of Intelligence and Information Systems
• /
• v.16 no.4
• /
• pp.173-193
• /
• 2010

The World Wide Web is transitioning from being a mere collection of documents that contain useful information toward providing a collection of services that perform useful tasks. The emerging Web service technology has been envisioned as the next technological wave and is expected to play an important role in this recent transformation of the Web. By providing interoperable interface standards for application-to-application communication, Web services can be combined with component-based software development to promote application interaction and integration within and across enterprises. To make Web services for service-oriented computing operational, it is important that Web services repositories not only be well-structured but also provide efficient tools for an environment supporting reusable software components for both service providers and consumers. As the potential of Web services for service-oriented computing is becoming widely recognized, the demand for an integrated framework that facilitates service discovery and publishing is concomitantly growing. In our research, we propose a framework that facilitates Web service discovery and publishing by combining clustering techniques and leveraging the semantics of the XML-based service specification in WSDL files. We believe that this is one of the first attempts at applying unsupervised artificial neural network-based machine-learning techniques in the Web service domain. We have developed a Web service discovery tool based on the proposed approach using an unsupervised artificial neural network and empirically evaluated the proposed approach and tool using real Web service descriptions drawn from operational Web services repositories. We believe that both service providers and consumers in a service-oriented computing environment can benefit from our Web service discovery approach.

• The KIPS Transactions:PartD
• /
• v.11D no.3
• /
• pp.671-682
• /
• 2004

As new and diverse information technologies are being introduced and software complexity is increased, software development cost and efforts are also sharply increased. Component-Based Development (CBD) technology is appealing as a new way to reduce the cost and effort by increasing reusability and maintainability. Component in CBD has variability infernally which enables customization of the component within the specific domain. A component user can easily set up internally variability parts though Required interface which is provided by the component. Enterprise JavaBeans (EJB) is utilized as a commercial standard to implement Java-based components. However, EJB constructs are limited in directly implementing Required interfaces of coarse-grained components［8］. In this paper, we define Required interface and propose interface-storage technique, class-storage technique, interface-generation technique, and Plug -in technique for implementing required interface of component. Interface-storage technique stores variable value in Required interface and class-storage technique take the Bean containg variability as Required interface without modification of component model. Interface-generation technique generates new Bean which takes the role of Required interface for component variability and Plug-in technique sets up component variability that component user plugged-in variable part externally The proposed four techniques conform to the semantics of CBD component interface and enable the implementation of high quality components.

• BMB Reports
• /
• v.53 no.6
• /
• pp.329-334
• /
• 2020

Inflammasomes are cytosolic, multiprotein complexes that act at the frontline of the immune responses by recognizing pathogen- or danger-associated molecular patterns or abnormal host molecules. Mesenchymal stem cells (MSCs) have been reported to possess multipotency to differentiate into various cell types and immunoregulatory effects. In this study, we investigated the expression and functional regulation of NLR Family Pyrin Domain Containing 3 (NLRP3) inflammasome in human umbilical cord blood-derived MSCs (hUCB-MSCs). hUCB-MSCs expressed inflammasome components that are necessary for its complex assembly. Interestingly, NLRP3 inflammasome activation suppressed the differentiation of hUCB-MSCs into osteoblasts, which was restored when the expression of adaptor proteins for inflammasome assembly was inhibited. Moreover, the suppressive effects of MSCs on T cell responses and the macrophage activation were augmented in response to NLRP3 activation. In vivo studies using colitic mice revealed that the protective abilities of hUCB-MSCs increased after NLRP3 stimulation. In conclusion, our findings suggest that the NLRP3 inflammasome components are expressed in hUCB-MSCs and its activation can regulate the differentiation capability and the immunomodulatory effects of hUCB-MSCs.

• Geophysics and Geophysical Exploration
• /
• v.18 no.1
• /
• pp.1-8
• /
• 2015

To estimate the shear-wave velocity (${\nu}_s$ beneath the OTVZ seismic station on Ngauruhoe volcano in New Zealand, we calculated receiver functions (RFs) using 127 teleseismic data ($Mw{\geq}5.5$) with high signal-to-noise ratios recorded during November 11, 2011 to September 11, 2013. The genetic inversion algorithms was applied to 21 RFs calculated by the iterative time-domain deconvolution method. In the 1-D ${\nu}_s$ model derived by the inversion, the Moho is observed at a 14 km depth, marked by a ${\nu}_s$ transition from 3.7 km/s to 4.7 km/s. The average ${\nu}_s$ of the overlying crust is 3.4 km/s, and the average ${\nu}_s$ of a greater than 9-km thick low-velocity layer (LVL) in the lower crust is 3.1 km/s. The LVL becomes thinner with increasing distance from the station. Another LVL thicker than 10 km with ${\nu}_s$ less than 4.3 km/s is found in the upper mantle. Those LVLs in the lower crust and the upper mantle and the relatively thin crust might be related to the magma activity caused by the subducting Pacific plate.

• Journal of Digital Convergence
• /
• v.11 no.10
• /
• pp.59-69
• /
• 2013

20s country employment rate to 57% in the last four years, is staying low. College graduates in the 25-29 year old crowd real unemployment rate is over 20% youth unemployment, the analysis problem is serious enough. Accordingly, activation of Cooperation, which addresses the issues of these countries now being recognized as the primary means, and in the future, government policy and national indicators to be given more attention and support seems clear. Government from 2012 'Cooperation Development Project leading universities' (LINC: Leaders in INdustry-university Cooperation) by promoting companies that require manpower, actual research and development and technology transfer to enable the University - Industry - between research institutions based on organic correlated with local universities and industrial growth model for the creation of a variety of leading industry cooperation. has to diffuse. This background, the aim of this study at the University of excavation site close-type talent. Fostering curriculum development are essential to the field on the form will close. In this study, major department (Department) conducted by major research based on the results of the curriculum needs to spread the field for Cooperation closely analyze the curriculum and Annual type, domain-specific promotion measures proposed.

• Journal of the Korean Chemical Society
• /
• v.57 no.6
• /
• pp.821-829
• /
• 2013

The purpose of this study was to develop 'Science History Based Chemist Inquiry Program' for the science-gifted in high school and investigated the application effects on the understanding toward the nature of science, scientific attitudes, and science career orientation. This study was conducted with one class of 17 scientifically gifted high school students, and the 'Science History Based Chemist Inquiry Program' was applied during 22 class hours. The results of this study were as follows: First, the science gifted students' understanding toward the nature of science showed a statistically significant improvement (approximate significance probability .005). Property of the scientific model, sub-domain of the nature of science, is significantly improved (approximate significance probability .029). This result show that 'Science History Based Chemist Inquiry Program' can improve the scientifically gifted high school students' perception about nature of science. Second, the scientifically gifted students' scientific attitude wasn't changed significantly. However, the openness and creativity in the sub-domains of the scientific attitude showed a statistically significant improvement (p<.05). Third, science career orientation of science-gifted did not show a statistically significant change. But the value of the level of awareness about the science career has been significantly improved (p<.05).