• Title/Summary/Keyword: Dolichos lablab L.

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Effect of Jebikong(Dolichos lablab L.) Leaves Extract on Enzyme Activities in Mice Blood (제비콩(Dolichos lablab L.) 잎 추출물이 쥐의 혈청효소활성에 미치는 영향)

  • 김용욱;최우철;신미경;한성희;임세진;김명애
    • Korean Journal of Organic Agriculture
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    • v.9 no.3
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    • pp.85-92
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    • 2001
  • The effect of Dolichos lablab leave extract on enzyme activities in mice blood was investigated in this study to clarify the new useful application of the Plant leaves. There were not significant differences in the enzyme activities in mice blood among treatment fed with the leave extract and non fed control. The feeding treatment with the extract showed a tendency to activity compared to the nun fed control. The activities of glutamate oxaloacetate transaminase, glutamate pyruvate transaminase and lactate dehydrogenase in mice blood at the treatment fed with lead and the leave extract were significantly low compared to their activities at the non fed treatment with the extract, respectively. The choline esterase activity was high at the leave extract feeding. The cadmium dietary treatment showed the same result as the lead treatment. In conclusion, the physiological function of the Jebikong leave was significantly in creased when the mice was stressed by the hear metal intake. Therefore, the plant leave extract would consider the reduction of heart metal effect.

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Purification and Identification of Antioxidant Compounds from Dolichos lablab L. Seeds (백편두의 항산화 물질 분리 및 동정)

  • Kwon, Nam Woo;Kim, Jae Yeon;Cho, Yong Beom;Hwang, Bang Yeon;Kim, Jun Gu;Woo, Sun Hee;Lee, Moon Soon
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.6
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    • pp.419-426
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    • 2019
  • Background: This study aimed to identify antioxidant compounds from the seeds of Dolichos lablab L. by bioassay-guided isolation and recrystallization. Methods and Results: The water layer of D. lablab L. seed extract inhibits intracellular reactive oxygen species (ROS) expressing the 2',7'-dichlorofluorescein diacetate (DCF-DA), Cu/Zn superoxide dismutase (SOD) and catalase genes, as determined by quantitative real-time PCR (qRT-PCR). Two compounds were purified from the water layer of the seeds of D. lablab L. using column chromatography and prep-high performance liquid chromatography (HPLC). Using nuclear magnetic resonance (NMR) and electrospray Ionization mass spectrometry (ESI-MS), their chemical structures were identified as 5-[(2-acetyl-2,3-dihydro-1H-indazol-1-yl)carbonyl]-4,5-dihydro-3H-furan-2-one (C14H14N2O4) and stachyose. Conclusions: Two active antioxidant compounds were purified from the seed extract of D. lablab L. seed extract and the structures of these compounds were identified as C14H14O4N2 and stachyose.

Two cases of Improved Diarrhea due to Enteral Nutrition in Oriental Medical Treatment (비위관 급식환자에서의 설사 치험 2례)

  • 임중화;유종민;장선영;한숙영;박석규;홍상선;김진성;윤상협;류봉하
    • The Journal of Korean Medicine
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    • v.24 no.3
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    • pp.192-198
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    • 2003
  • Diarrhea is defined as increased volume and frequency of stools, increased liquidity and decreased consistency of stools. Enteral nutrition is suited to the patient in a state of normal gastro-intestinal function but insufficient oral nutrition due to neurologic injury. Of many problems raised by enteral nutrition, diarrhea is the most common complication and raises severe clinical problems such as dehydration, disproportion of electrolytes, metabolic acidosis, bed sores, etc. We report two cases of diarrhea due to enteral nutrition treated with Dolichos lablab L. preparation added to herb-med prescription in stroke patients. After this treatment, the frequency of diarrhea decreased and general condition improved.

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Effects of Scarification and Water Soaking Treatment on Germination of Hard-Seeded Legumes (두과 작물의 경실종자 발아촉진에 대한 종피연화처리의 효과)

  • Kim, Seok-Hyeon;Chang, Mi-Ha;Chung, Jong-Il;Shim, Sang-In
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.54 no.3
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    • pp.320-326
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    • 2009
  • The crops showing hard seed character have high seed viability after seed storage for long period. The low germination rate due to hard seed coat, however, cause a problem of low seedling establishment in field condition. Three legumes used in the experiment, lablab bean (Dolichos lablab L.), asparagus bean (Vigna sesquipedalis L. Fruwirth), and soybean (Glycine max L. Merr.), showed low germination rate (26, 17, and 5%, respectively) due to thick and hard seed coat. In this study artificial treatment for breaking dormancy was tested in hard seeds. The effect of proper treatment was various depending on species. The germination rate of lablab bean was highly improved up to 94% by soaking into water for 24 hours. In the case of asparagus bean, the rate was increased up to 90% by soaking for eight hours near boiling water until it cools. The germination rate of small hard seed soybean was increase to 96% by soaking into concentrated sulfuric acid for 10 minutes. Ultrastructural change revealed by scanning electron microscope (SEM) reflects that the structure of micropyle was changed and water uptake was facilitated with all treatments tested in the experiment. Especially, sulfuric acid treatment resulted in the degradation of micropylar tissue. These results demonstrate that the artificial treatment including sulfuric acid and (hot) water soaking treatment for promoting water uptake can be applied to improve seed germination in legume seed with thick and hard seed coat.

Research on vasorelaxant effects of 20 Korean native plants (국내 자생식물 20종의 혈관이완 효능에 대한 실험연구)

  • Bumjung Kim
    • The Korea Journal of Herbology
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    • v.38 no.5
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    • pp.39-47
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    • 2023
  • Objectives : The objective of present study was to investigate the vasorelaxant effects of 20 Korean native plants on isolated rat thoracic aorta precontracted with phenylephrine (PE). Methods : Dried 20 plant materials were extracted 3 times with water, ethanol, or methanol for 3h in the reflux apparatus at 70 ± 5℃. Male SD rats were anesthetized by ether inhalation, and their aorta rings were isolated and placed in 10 ㎖ Krebs Henseleit (KH) buffer. While using an isolated organ-chamber technique, the aorta rings were maintained by bubbling with a gas mixture of 95% O2-5% CO2 at 37℃. Changes in isometric tension of aorta rings were recorded via isometric transducers connected to a Powerlab Data Acquisition System. Results : Among the 20 native plants, Chrysanthemum indicum L. flower, Nelumbo nucifera Gaertn. rhizome, Schisandra chinensis (Turcz.) Baill. fruit, Anthriscus sylvestris (L.) Hoffm. root, Corydalis turtschaninovii Besser tuber, Corydalis decumbens (Thunb.) Pers. tuber, and Dolichos lablab L. seed showed significant vasorelaxant effect on the contraction of aorta rings induced by PE. In contrast, Mertensia maritima subsp. asiatica Takeda whole plant, Ajuga decumbens Thunb. whole plant, Trigonotis peduncularis (Trevis.) Benth. ex Baker & S.Moore whole plant, Dioscorea quinquelobate Thunb. rhizome, Allium microdictyon Prokh aerial part, Momordica charantia L. fruit, Carthamus tinctorius L. flower, and Clematis terniflora DC. root constricted more the aorta rings precontracted by PE Conclusion : These results suggest that the possibility as useful herbal resources for the development of functional foods or medicines for hypertension treatment.

A Study on Addition & Subtraction of Formula by Four Seasons (사시(四時)에 따른 처방(處方) 가가(加減)에 관한 고찰(考察))

  • Kim, Jin-Hyun;Eum, Dong-Myung;Kim, Sang-Kyun;Kim, Chul;Jang, Hyun-Chul;Yea, Sang-Jun;Song, Mi-Young
    • Journal of Korean Medical classics
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    • v.22 no.3
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    • pp.133-144
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    • 2009
  • Objective : We try to find out the literature and clinical evidences of seasonal formula through the analysis of the addition and subtraction of medical herbs in the formula according to the season. Method : In this study, we analyzed 122 seasonal prescriptions from "Dong-uibogam(東醫寶鑑)", "Bang-yakhappyeon(方藥合編)", "Jeongchijunsueng(證治準繩)", "Dangyesimbeop(丹溪心法)", "Uihakimmun(醫學入門)", "Uihakjeongjeon(醫學正傳)", "Gyeong-akjeonseo(景岳全書)" and "Taepyeonghyeminhwajegukbang(太平惠民和劑局方)". Results : In spring, pungent-warm herbs such as the dried rhizome of cnidium officinale Makino, the dried root of SEdebouriella divaricata (Turcz.) Schischk. are used. In summer, bitter-cold such as the dried root of Scutellaria baicalensis Georgi, the dried rhizome of Coptis chinensis Franch. or pungent herbs such as the dried aerial part of Elsholtzia splendens Nakai, the dried ripe seed of Dolichos lablab L. are added. In fall and winter, pungent-bitter-hot herbs such as the dried ripe fruit of Evodia rutaecarpa Benth., the dried rhirome of Zingiber officinale Rosc. are used. Conclusions : In this paper, we have shown that several medical herbs could be added and subtracted in the formula according to the season because they have the seasonal characteristic Gi or treat seasonal pathogens.

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